Report on Marine Mammal Unusual Mortality Event UMESE0501Sp: Multispecies Mass Stranding of Pilot Whales (Globicephala macrorhynchus), Minke Whale (Balaenoptera acutorostrata), and Dwarf Sperm Whales (Kogia sima) in North Carolina on 15-16 January 2005

On 15-16 January 2005, three offshore species of cetaceans (33 short-finned pilot whales, Globicephala macrorhynchus, one minke whale, Balaenoptera acutorostrata, and two dwarf sperm whales, Kogia sima) stranded alive on the beaches of North Carolina. The pilot whales stranded near Oregon Inlet, the minke whale in northern North Carolina, and the dwarf sperm whales near Cape Hatteras. Live strandings of three species in one weekend was unique in North Carolina and qualified as an Unusual Mortality Event. Gross necropsies were conducted on 16-17 January 2005 on 27 pilot whales, two dwarf sperm whales, and the minke whale. Samples were collected for clinical pathology, parasitology, gross pathology, histopathology, microbiology and serology. There was variation in the number of animals sampled for each collection type, however, due to carcasses washing off the beach or degradation in carcass condition during the course of the response. Comprehensive histologic examination was conducted on 16 pilot whales, both dwarf sperm whales, and the minke whale. Limited organ or only head tissue suites were obtained from nine pilot whales. Histologic examination of tissues began in February 2005 and concluded in December 2005 when final sampling was concluded. Neither the pilot whales nor dwarf sperm whales were emaciated although none had recently ingested prey in their stomachs. The minke whale was emaciated; it was likely a dependent calf that became separated from the female. Most serum biochemistry abnormalities appear to have resulted from the stranding and indicated deteriorating condition from being on land for an extended period. Three pilot whales had clinical evidence of pre-existing systemic inflammation, which was supported by histopathologic findings. Although gross and histologic lesions involving all organ systems were noted, consistent lesions were not observed across species. Verminous pterygoid sinusitis and healed fishery interactions were seen in pilot whales but neither of these changes were causes of debilitation or death. In three pilot whales and one dwarf sperm whale there was evidence of clinically significant disease in postcranial tissues which led to chronic debilitation. Cardiovascular disease was present in one pilot whale and one dwarf sperm whale; musculoskeletal disease and intra-abdominal granulomas were present in two pilot whales. These lesions were possible, but not definitive, causal factors in the stranding. Remaining lesions were incidental or post-stranding. The minke whale and three of five tested pilot whales had positive morbillivirus titers (≥1:8 with one at >1:256), but there was no histologic evidence of active viral infection. Parasites (nematodes, cestodes, and trematodes) were collected from 26 pilot whales and two dwarf sperm whales. Sites of collection included stomach, nasal/pterygoid, peribullar sinuses, blubber, and abdominal cavity. Parasite species, locations and loads were within normal limits for free-ranging cetaceans and were not considered causative for the stranding event. Gas emboli lesions which were considered consistent with or diagnostic of sonarassociated strandings of beaked whales or small cetaceans were not found in the whales stranded as part of UMESE0501Sp. Twenty-five heads were examined with nine specific anatomic locations of interest: extramandibular fat, intramandibular fat, auditory meatus, peribullar acoustic fat, peribullar soft tissue, peribullar sinus, pterygoid sinus, melon, and brain. The common finding in all examined heads was verminous pterygoid sinusitis. Intramandibular adipose tissue reddening, typically adjacent to the vascular plexus, was observed in some individuals and could represent localized hemorrhage resulting from vascular rete rupture, hypostatic congestion, or erythrocyte rupture during the freeze/thaw cycle. One cetacean had peracute to acute subdural hemorrhage that likely occurred from thrashing on the beach post-stranding, although its occurrence prior to stranding cannot be excluded. Information provided to NMFS by the U.S. Navy indicated routine tactical mid-frequency sonar operations from individual surface vessels over relatively short durations and small spatial scales within the area and time period investigated. No marine mammals were detected by marine mammal observers on operational vessels; standard operating procedure for surface naval vessels operating mid-frequency sonar is the use of trained visual lookouts using high-powered binoculars. Sound propagation modeling using information provided to NMFS indicated that acoustic conditions in the vicinity likely depended heavily on position of the receivers (e.g., range, bearing, depth) relative to that of the sources. Absent explicit information on the location of animals meant that it was not possible to estimate received acoustic exposures from active sonar transmissions. Nonetheless, the event was associated in time and space with naval activity using mid-frequency active sonar. It also had a number of features in common (e.g., the “atypical” distribution of strandings involving multiple offshore species, all stranding alive, and without evidence of common infectious or other disease process) with other sonar-related cetacean mass stranding events. Given that this event was the only stranding of offshore species to occur within a 2-3 day period in the region on record (i.e., a very rare event), and given the occurrence of the event simultaneously in time and space with a naval exercise using active sonar, the association between the naval sonar activity and the location and timing of the event could be a causal rather than a coincidental relationship. However, evidence supporting a definitive association is lacking, and, in particular, there are differences in operational/environmental characteristics between this event and previous events where sonar has apparently played a role in marine mammal strandings. This does not preclude behavorial avoidance of noise exposure. No harmful algal blooms were present along the Atlantic coast south of the Chesapeake Bay during the months prior to the event. Environmental conditions, including strong winds, changes in upwelling- to downwelling-favorable conditions, and gently sloping bathymetry, were consistent with conditions which have been correlated with other mass strandings. In summary, we did not find commonality in gross and histologic lesions that would indicate a single cause for this stranding event. Three pilot whales and one dwarf sperm whale had debilitating conditions identified that could have contributed to stranding, one pilot whale had a debilitating condition (subdural hemorrhage) that could have been present prior to or resulting from stranding. While the pilot and dwarf sperm whale strandings may have had a common cause, the minke whale stranding was probably just coincidental. On the basis of examination of physical evidence in the affected whales, however, we cannot definitively conclude that there was or was not a causal link between anthropogenic sonar activity or environmental conditions (or a combination of these factors) and the strandings. Overall, the cause of UMESE0501Sp in North Carolina is not and likely will not be definitively known. (PDF contains 240 pages)

Tunas and tuna fisheries of the World: an annotated bibliography, 1930-1953

This bibliography attempts to list, with descriptive annotations and a subject index, important literature published between 1930 and 1953 dealing with the tunas and their fisheries in all parts of the world. It is thus a continuation of Corwin's (1930) work, which extended with similar scope through 1929, and an extension of Shimada's (1951), which was limited to the biology of Pacific tunas. The tunas with which it deals are those fishes customarily so-called in commercial parlance and usually classified in the genera Thunnus, Neothunnus, Parathunnus, Germo, Katsuwonus, Euthynnus and Auxis and their various synonyms. All aspects of the biology of the tunas are dealt with, as are descriptions and histories of all types of tuna fisheries, commercial and exploratory tuna fishing methods and results, fishing gear, catch statistics, and fishery management, but processing technology, economics and marketing, folklore, and purely literary references have been excluded.

Falsche Fische – ein Bericht über die Schwierigkeiten der Identifizierung einer „Seezunge“

In the last years German food control laboratories have established proof of a significant number of cases of incorrectly labelled flatfish on the German market. A flatfish offered as sole (Solea vulgaris) in Southern Germany served as an example for mislabelled flatfish and for the difficulties food control laboratories may encounter and to identify products of unknown origin. Morphometric and meristic examination, as well as isoelectric focusing of sarcoplasmic proteins, PCR-based DNA-analysis failed to identify the fish. By using these methods, it only could be excluded that the fish belonged to the species of Solea vulgaris or another described flatfish species. DNA sequencing of an amplicon gave a sequence identical to a sequence in GenBank, which, however, turned out to be incorrectly assigned to Solea vulgaris. More research about characterization and identification of tropical flatfish is recommended, because of the growing importance of these fishes for the European market.

An annotated bibliography on the biology and fishery of the skipjack tuna, Katsuwonis pelamis, of the Pacific Ocean

ENGLISH: The skipjack tuna, Katsuwonus pelamis is an important resource of the tropical and subtropical waters of the world ocean. Fishermen of many countries exploit this resource; at the present time, the annual world catch is approximately 200 thousand metric tons. Many fishery experts believe that the skipjack is not being fully utilized while stocks of other tunas are being fished, in some areas, at levels exceeding their maximum sustainable yields. In addition to the importance of skipjack as a commercial fish and as a source of food, there is a small but expanding recreational fishery in some countries bordering the Pacific. This bibliography provides a list of publications pertaining to the biology and fishery of the Pacific skipjack tuna. Papers concerned with food technology, food chemistry, radio-chemistry, and certain other subjects are excluded. The main sources for our publication have been the existing bibliographies of tunas, which are listed and indexed accordingly. In addition, reports of various marine laboratories and other scientific organizations have been checked; these are too numerous to list. We are fairly confident that all major works pertaining to skipjack tuna in the Pacific, printed prior to the end of 1966, appear in this bibliography. Only reports considered to be in permanent form are included. Annotations are based on actual examination of each of the entries listed here. The annotations do not evaluate a paper but serve rather to give a more precise idea of its contents if not revealed by the title alone. If the title sufficed in this respect, no annotation was prepared. A relatively small number of works believed to contain information pertinent to our bibliography could not be examined, but a list of such papers is provided. SPANISH: El atún barrilete, Katsuwonus pelamis, es un recurso importante de las aguas tropicales y subtropicales del océano mundial. Los pescadores de varios países explotan este recurso; actualmente, la captura mundial anual es aproximadamente de 200,000 toneladas métricas. Muchos expertos en la pesquería creen que el barrilete no es utilizado completamente, mientras los stocks de otros atunes son pescados en algunas áreas a niveles que exceden su rendimiento máximo sostenible. Además de la importancia del barrilete como pez comercial y como fuente de alimento, existe una pesquería pequeña recreativa que se está desarrollando en algunos países colindantes con el Pacífico. Esta bibliografía suministra una lista de publicaciones correspondientes a la biología y pesquería del atún barrilete en el Pacífico. Estudios referentes a la tecnología alimenticia, química alimenticia, radioquímica y ciertos otros sujetos son excluídos. Las fuentes principales correspondientes a nuestra publicación han sido las bibliografías existentes sobre atunes, las cuales están enumeradas y catalogadas de acuerdo. Además, se han examinado los informes de varios laboratorios marítimos y los de otras organizaciones científicas; éstos son demasiado numerosos para enumerar. Estamos bastante seguros de que todos los trabajos principales correspondientes al atún barrilete del Pacífico, editados antes de terminar el año de 1966, aparecen en esta bibliografía. Se incluyen únicamente los informes que se consideran permanentes. Las anotaciones se basan en el examen actual de cada una de las entradas aquí referidas. Las anotaciones no evaluan un estudio, pero sirven más bien para dar una idea más precisa de su contenido si el título por sí mismo no lo explica. No se preparó ninguna anotación si el título a este respecto era suficiente. Un número relativamente pequeño de trabajos que se cree tengan información pertinente a nuestra bibliografía no pudo ser examinado, pero se suministra una lista de tales estudios. (PDF contains 227 pages.)

Entwicklung einer einfachen und schnellen Analysenmethode für Kohlenmonoxid in Fisch und Durchführung orientierender Untersuchungen

Treatment with gases containing CO, misleadingly called “tasteless smoke” or “clear smoke”, prior to freezing of fish like tuna, tilapia and swordfish (to name only a few of these species that are characterised by an intensive coloration of flesh) stabilise the fresh red colour of muscle also after thawing and suggest consumers non-existing freshness. In the European Union, carbon monoxide is excluded from being a permitted additive and therefore this handling is not allowed. Notwithstanding of the clear legal position, producers and traders are trying to establish CO treated fish on the market. In the case of taking legal measures the food control laboratories have to provide evidence that fish has been treated by CO and therefore a respective method is necessary. The method of determination of carbon monoxide in fish flesh presently applied requires considerable material and mechanistic effort to detect CO by GC after catalytic transformation into methane. The aim of our work was a direct detection of CO using suitable sensor technology. Mechanistic requirements and results of preliminary investigations to detect carbon monoxide in fish flesh will be described.

Experiments on downstream movement of recently emerged trout and salmon parr in Grassholme channels, 1983-1985

A number of authors have described the manner in which young salmonids, soon after emergence from the gravel, set up and defend territories. This leads to mortality or downstream displacement of the individuals which are unable to acquire territories and is widely accepted as the main method of population regulation amongst young salmonids. In some field experiments the fish were constrained in screened reaches and the option of downstream dispersal for the surplus fry was thus excluded. In order to explore some aspects of downstream dispersal more closely under conditions which gave more control than is obtained in a natural stream, four experimental channels were set up at Grassholme reservoir in Teesdale. The report describes the results of investigations on the timing and rate of downstream movement of young brown trout (Salmo trutta L.) and Atlantic salmon (Salmo salar L.) out of experimental channels, with special reference to the effect of water velocity on the rate of ”emigration”.

The kingdom of the shore: achievement of good ecological potential in reservoirs

The European Water Framework Directive requires member states to restore aquatic habitats to good ecological status (quality) by 2015. Good ecological status is defined as slightly different from high status, which, according to the Directive, means negligible human influence. This poses problems enough for restoration of natural habitats but artificial reservoirs are not excluded from the Directive. They must be restored to good ecological potential. The meaning of good ecological status is linked to that of 'high' ecological status, the pristine reference condition for aquatic habitats under the Directive. From the point of view of an ecologist, this is taken to mean the presence of four fundamental characteristics: nutrient parsimony, characteristic biological and physical structure, connectivity within a wider system and adequate size to give resilience of the biological communities to environmental change. These characteristics are strongly interrelated. Ecological potential must bear some relationship to ecological status but since the reference state for ecological quality is near absence of human impact, it is difficult to see how the criteria for ecological status can be applied to a completely man-made entity where the purpose of the dam is deliberately to interfere with the natural characteristics of a river or former natural lake. Rservoirs are disabled lakes, ususally lakcing the diversity and function provided by a littoral zone. Nonetheless, pragmatic approaches to increasing the biodiversity of reservours are reviewed and conclusions drawn as to the likely effectivemess of the legislation.

Comparing the identification of southern California juvenile rockfishes (genus Sebastes spp.) by restriction site analysis of the mitochondrial ND3/ND4 region and by morphological characteristics

Rockfish (Sebastes spp.) juveniles are often difficult to identify by using morphological characters. This study independently applies morphological characters and a key based on mitochondrial restriction site variation to identify juvenile rockf ishes collected in southern California during juvenile rockfish surveys. Twenty-four specimens of Sebastes were examined genetically without knowledge of the morphological assignment. Seventeen fish were identified genetically as S. semicinctus, S. goodei, S. auriculatus, S. jordani, S. levis, S. rastrelliger, and S. saxicola. Identities for the remaining fish were narrowed to two or three species: 1) three fish were either S. carnatus or S. chrysomelas; 2) one fish was either S. chlorosticus, S. eos, or S. rosenblatti; and 3) three fish could have been either S. hopkinsi or S. ovalis, the latter for which we now have distinguishing mitochondrial markers. The genetic and morphological assignments concurred except for the identity of one fish that could only be narrowed down to S. hopkinsi or S. semicinctus by using morphological characters. Genetics excluded more species from multispecies groupings than did the morphological approach, especially species within the subgenus Sebastomus. Species in the genetically unresolvable groups may be similar because of recent divergence or because of interspecies introgression.

Occurrence and distribution of fish species off Yemen (Gulf of Aden and Arabian Sea)

The species list is drawn from an analysis of catches taken by Sumalian and Russian trawlers in the Gulf of Aden and the Arabian Sea between 1985 and 1990. The southern coastline of the Republic of Yemen has been divided into 7 areas, including waters around Socotra Island. The average depth of each trawl was recorded in 50 m increments. Non-appearance of the species in the area does not mean that the species do not occur in that area or depth, merely that it was not recorded in any of the samples analyzed. Specimens that could not be identified to species level have been excluded. A total of 195 species from 75 families was recorded and is summarized. Most of the identification of species was from FAO species identification literature. Confirmation of some species and usage of common names is from ICLARM's FishBase and Al Sedfy, et al. (1982).

Transforming aquatic agricultural systems towards gender equality: a five country review

Aquatic agricultural systems (AAS) are systems in which the annual production dynamics of freshwater and/or coastal ecosystems contribute significantly to total household income. Improving the livelihood security and wellbeing of the estimated 250 million poor people dependent on AAS in Bangladesh, Cambodia, the Philippines, the Solomon Islands and Zambia is the goal of the Worldfish Center-led Consortium Research Program (CRP), “Harnessing the development potential of aquatic agricultural systems for development.” One component expected to contribute to sustainably achieving this goal is enhancing the gender and wider social equity of the social, economic and political systems within which the AAS function. The CRP’s focus on social equity, and particularly gender equity, responds to the limited progress to date in enhancing the inclusiveness of development outcomes through interventions that offer improved availability of resources and technologies without addressing the wider social constraints that marginalized populations face in making use of them. The CRP aims to both offer improved availability and address the wider social constraints in order to determine whether a multi-level approach that engages with individuals, households and communities, as well as the wider social, economic and political contexts in which they function, is more successful in extending development’s benefits to women and other excluded groups. Designing the research in development initiatives to test this hypothesis requires a solid understanding of each CRP country’s social, cultural and economic contexts and of the variations across them. This paper provides an initial input into developing this knowledge, based on a review of literature on agriculture, aquaculture and gender relations within the five focal countries. Before delving into the findings of the literature review, the paper first justifies the expectation that successfully achieving lasting wellbeing improvements for poor women and men dependent on AAS rests in part on advances in gender equity, and in light of this justification, presents the AAS CRP’s conceptual framew

Die vertikale Verteilung von Heringslarven im Greifswalder Bodden

Kurzfassung Im Rahmen einer Bachelorarbeit wurde untersucht, ob die Larven des frühjahrslaichenden Herings der westlichen Ostsee im Greifswalder Bodden eine inhomogene vertikale Verteilung aufweisen, da in bisherigen Studien aufgrund der niedrigen Gewässertiefe des Boddens von hydrografischer Durchmischung und somit homogener Ichthyoplanktonverteilung ausgegangen wurde. Dazu wurden zwei Nullhypothesen überprüft, eine, die annimmt, dass Heringslarven allgemein im Greifswalder Bodden homogen verteilt sind, eine zweite, nach der auch verschiedenen Längenklassen der Heringe homogen verteilt sind. Die Proben, die Mitte April an drei verschiedenen Stationen im Greifswalder Bodden genommen wurden, zeigten signifikante Unterschiede der Larvenabundanzen allgemein zwischen den beprobten Tiefenstufen als auch der Längenklassen zwischen den Tiefenstufen, sodass beide Nullhypothesen abzulehnen sind. Eine homogene Verteilung der Heringslarven kann ausgeschlossen werden. Abstract Larvae of the Western Baltic Spring Spawning Herring were sampled in the Greifswalder Bodden in the course of a Bachelor Thesis to investigate whether they are inhomogeneously vertically distributed. Previous research assumed homogeneous vertical distribution because of the shallowness of the Greifswalder Bodden due to hydrographical mixing of the water and the ichthyoplankton. Two null hypotheses were tested, one which presumes even vertical distribution of the herring larvae and another which presumes even vertical distribution within different length classes in the Greifswalder Bodden. Sampling took place at three different stations in the Greifswalder Bodden during April and results showed significant differences of the larvae abundances between the sampled depths and also significant differences of the length classes between the sampled depths. Therefore both null hypotheses can be rejected and a homogeneous vertical distribution of the herring larvae in the Greifswalder Bodden can be excluded.

An integrated biogeographic assessment of reef fish populations and fisheries in Dry Tortugas: Effects of No-take Reserves

The Tortugas Integrated Biogeographic Assessment presents a unique analysis of demographic changes in living resource populations, as well as societal and socioeconomic benefits that resulted from the Tortugas Ecological Reserves during the first five years after their implementation. In 2001, state and federal agencies established two no-take reserves within the region as part of the Florida Keys National Marine Sanctuary. The northern reserve (Tortugas Ecological Reserve North) was established adjacent to the Dry Tortugas National Park, which was first declared a national monument in 1935. The reserves were designed to protect a healthy coral reef ecosystem that supports diverse faunal assemblages and fisheries, serves as important spawning grounds for groupers and snappers, and includes essential feeding and breeding habitats for seabirds. The unique ecological qualities of the Tortugas region were recognized as far back as 1850, and it remains an important ecosystem and research area today. The two main goals of the Tortugas Ecological Reserve Integrated Ecological Assessment were: 1) to determine if demographic changes such as increases in abundance, average size and spawning potential of exploited populations occurred in the Tortugas region after reserve implementation; and 2) whether short-term economic losses occurred to fishers displaced by the reserve. This project utilized a biogeographic approach in which information on the physical features (i.e., habitat) and oceanographic patterns were first used to determine the spatial distribution of selected fish populations within and outside the Tortugas Ecological Reserve. Before-and-after reserve implementation comparisons of selected fish populations were then conducted to determine if demographic changes occurred in reef fish assemblages. These comparisons were done for the Tortugas region and also for a subset of available habitats within the Tortugas Ecological Reserve Study Area. Social and economic impacts of the reserves were determined through: 1) analyses of commercial landings and revenues from fishers, operating in the Tortugas region before and after reserve implementation and 2) surveys of recreational tour guides. Analyses of the commercial landings and revenues excluded areas inside Dry Tortugas National Park because commercial fishing has been prohibited within park boundaries since 1992. Key findings and outcomes of this integrated ecological assessment are organized by chapter and listed below.

Assessment of the performance of beach management units (BMUs) on Lakes Victoria, Albert, Kyoga and George

Until the late 1990s the fisheries of Ugandan lakes had been managed by government where stakeholders were excluded from the decision-making process. In order to involve other stakeholders, co-management was adopted. Operationalising Co-management on landing sites has led to the formation of BMUs at gazetted landing sites. A BMU is made up of a BMU assembly and the BMU committee that it elects. A BMU committee should be: 30% boat owners; 30% boat barias 30% including fish processors, boat makers, local gear makers and repairers, fishing input dealers and managers and 10% fish mongers/traders; and if possible, 30% women. To operate at a particular landing site, one must be registered with the BMU. The BMU assembly is the supreme organ of a BMU empowered to elect, approve and remove the BMU committee

Marine Vertebrates and Low Frequency Sound: Technical Report for LFA EIS

Over the past 50 years, economic and technological developments have dramatically increased the human contribution to ambient noise in the ocean. The dominant frequencies of most human-made noise in the ocean is in the low-frequency range (defined as sound energy below 1000Hz), and low-frequency sound (LFS) may travel great distances in the ocean due to the unique propagation characteristics of the deep ocean (Munk et al. 1989). For example, in the Northern Hemisphere oceans low-frequency ambient noise levels have increased by as much as 10 dB during the period from 1950 to 1975 (Urick 1986; review by NRC 1994). Shipping is the overwhelmingly dominant source of low-frequency manmade noise in the ocean, but other sources of manmade LFS including sounds from oil and gas industrial development and production activities (seismic exploration, construction work, drilling, production platforms), and scientific research (e.g., acoustic tomography and thermography, underwater communication). The SURTASS LFA system is an additional source of human-produced LFS in the ocean, contributing sound energy in the 100-500 Hz band. When considering a document that addresses the potential effects of a low-frequency sound source on the marine environment, it is important to focus upon those species that are the most likely to be affected. Important criteria are: 1) the physics of sound as it relates to biological organisms; 2) the nature of the exposure (i.e. duration, frequency, and intensity); and 3) the geographic region in which the sound source will be operated (which, when considered with the distribution of the organisms will determine which species will be exposed). The goal in this section of the LFA/EIS is to examine the status, distribution, abundance, reproduction, foraging behavior, vocal behavior, and known impacts of human activity of those species may be impacted by LFA operations. To focus our efforts, we have examined species that may be physically affected and are found in the region where the LFA source will be operated. The large-scale geographic location of species in relation to the sound source can be determined from the distribution of each species. However, the physical ability for the organism to be impacted depends upon the nature of the sound source (i.e. explosive, impulsive, or non-impulsive); and the acoustic properties of the medium (i.e. seawater) and the organism. Non-impulsive sound is comprised of the movement of particles in a medium. Motion is imparted by a vibrating object (diaphragm of a speaker, vocal chords, etc.). Due to the proximity of the particles in the medium, this motion is transmitted from particle to particle in waves away from the sound source. Because the particle motion is along the same axis as the propagating wave, the waves are longitudinal. Particles move away from then back towards the vibrating source, creating areas of compression (high pressure) and areas of rarefaction (low pressure). As the motion is transferred from one particle to the next, the sound propagates away from the sound source. Wavelength is the distance from one pressure peak to the next. Frequency is the number of waves passing per unit time (Hz). Sound velocity (not to be confused with particle velocity) is the impedance is loosely equivalent to the resistance of a medium to the passage of sound waves (technically it is the ratio of acoustic pressure to particle velocity). A high impedance means that acoustic particle velocity is small for a given pressure (low impedance the opposite). When a sound strikes a boundary between media of different impedances, both reflection and refraction, and a transfer of energy can occur. The intensity of the reflection is a function of the intensity of the sound wave and the impedances of the two media. Two key factors in determining the potential for damage due to a sound source are the intensity of the sound wave and the impedance difference between the two media (impedance mis-match). The bodies of the vast majority of organisms in the ocean (particularly phytoplankton and zooplankton) have similar sound impedence values to that of seawater. As a result, the potential for sound damage is low; organisms are effectively transparent to the sound – it passes through them without transferring damage-causing energy. Due to the considerations above, we have undertaken a detailed analysis of species which met the following criteria: 1) Is the species capable of being physically affected by LFS? Are acoustic impedence mis-matches large enough to enable LFS to have a physical affect or allow the species to sense LFS? 2) Does the proposed SURTASS LFA geographical sphere of acoustic influence overlap the distribution of the species? Species that did not meet the above criteria were excluded from consideration. For example, phytoplankton and zooplankton species lack acoustic impedance mis-matches at low frequencies to expect them to be physically affected SURTASS LFA. Vertebrates are the organisms that fit these criteria and we have accordingly focused our analysis of the affected environment on these vertebrate groups in the world’s oceans: fishes, reptiles, seabirds, pinnipeds, cetaceans, pinnipeds, mustelids, sirenians (Table 1).