Role of Aeromonas hydrophila in bacterial septicemia of cultured carps in Khouzestan Province and investigation on protectivity of bacterin (s) prepared from acute isolate(s) of it

Motile Aeromonas are the most common bacteria of freshwater in the world that cause disease in fish and other cold-blooded and warm-blooded hosts. Among this group of bacteria, Aeromonas hydrophila is important in causing complications such as fin rot, skin ulcers and lethal hemorrhagic septicemia in fish. Several virulence factors involved in the pathogenesis of Aeromonas hydrophila, including extracellular enzymes (protease, lipase, elastase, gelatinase and nuclease) and toxins. From the exotoxins, hemolysin, aerolysin and cytolytic enterotoxin play an important role in pathogenesis. Detection of virulence markers by PCR as a key component of determining the pathogenesis of the bacteria and using indigenous vaccines for better immunization against this disease is important. In this study, a total of 200 fanned carps (126 common carp. 39 silver carp and 35 of grass carp) with symptoms suspected aeromonas septicemia were isolated from Khouzestan province farms. 125 bacteria belong to Aeromonas genus detected by biochemical and PCR methods. 31 of all isolates recognized as Aeromonas hydrophila with biochemical methods, I6srRNA detection and Lipase genes. Results showed that the role of Aeromonas sp. and Aeromonas hydrophila in fish with disease symptoms were 62.5% and 15.5% respectively. By using specific primers, three virulence genes including hemolysin, aerolysine and cytolytic enterotoxin were detected in these confirmed isolates, that 18 isolates (58/06%) hemolysin positive (hlyA +), 16 isolates (51/61%) aerolysine positive (aerA+) and 23 isolates (74/19%) for cytolytic enterotoxin gene (act+) were positive. The result of present study showed that most of the confirmed isolates genotype was hlyA+ act- with frequency equal to 51/61%. For investigating the protection effect of acut strain of bacteria, UV inactivated bacterin was used.

Annual report 2003 - North Pacific Marine Science Organization (PICES). Twelfth meeting, Seoul, Republic of Korea, October 9-18, 2003

Report of Opening Session (pdf 58 KB) Report of Governing Council Meeting (pdf 244 KB) Report of 2003 interim Governing Council meeting Tenth Anniversary PICES Organization Review Report of the Finance and Administration Committee (pdf 102 KB) 2002 Auditor's report to the Organization Review of PICES Publication Program Reports of Science Board and Committees: Science Board/Governing Council interim meeting (pdf 81 KB) Science Board (pdf 95 KB) Study Group on PICES Capacity Building Biological Oceanography Committee (pdf 65 KB) Advisory Panel on Micronekton sampling gear intercalibration experiment Advisory Panel on Marine birds and mammals Fishery Science Committee (pdf 41 KB) Working Group 16 on Climate change, shifts to fish production, and fisheries management Marine Environmental Quality Committee (pdf 76 KB) Working Group 15 on Ecology of Harmful Algal Blooms (HABs) in the North Pacific Physical Oceanography and Climate Committee (pdf 70 KB) Working Group 17 on Biogeochemical data integration and synthesis Advisory Panel on North Pacific Data Buoy Technical Committee on Data Exchange (pdf 32 KB) Implementation Panel on the CCCC Program (pdf 64 KB) Nemuro Experimental Planning Team (NEXT) BASS Task Team (pdf 35 KB) Advisory Panel on Iron Fertilization Experiment MODEL Task Team (pdf 29 KB) MONITOR Task Team (pdf 30KB) REX Task Team (pdf 25 KB) Documenting Scientific Sessions (pdf 164 KB) List of Participants (pdf 60 KB) List of Acronyms (pdf 21 KB)

Characterization of Navassa National Wildlife Refuge: A preliminary report for NF-06-05 (NOAA ship "Nancy Foster", April 18-30, 2006)

Navassa is a small, undeveloped island in the Windward Passage between Jamaica and Haiti. It was designated a National Wildlife Refuge under the jurisdiction of the U.S. Fish and Wildlife Service in 1999, but the remote location makes management and enforcement challenging, and the area is regularly fished by artisanal fishermen from Haiti. In April 2006, the NOAA Center for Coastal Fisheries and Habitat Research conducted a research cruise to Navassa. The cruise produced the first high-resolution multibeam bathymetry for the area, which will facilitate habitat mapping and assist in refuge management. A major emphasis of the cruise was to study the impact of Haitian fishing gear on benthic habitats and fish communities; however, in 10 days on station only one small boat was observed with five fishermen and seven traps. Fifteen monitoring stations were established to characterize fish and benthic communities along the deep (28-34 m) shelf, as these areas have been largely unstudied by previous cruises. The fish communities included numerous squirrelfishes, triggerfishes, and parrotfishes. Snappers and grouper were also present but no small individuals were observed. Similarly, conch surveys indicated the population was in low abundance and was heavily skewed towards adults. Analysis of the benthic photoquadrats is currently underway. Other cruise activities included installation of a temperature logger network, sample collection for stable isotope analyses to examine trophic structure, and drop camera surveys to ground-truth habitat maps and overhead imagery. (PDF contains 58 pages)