Assessing the quality of water quality assessments: An analytical quality control protocol for benthic diatoms

In this paper, the background to the development of an analytical quality control procedure for the Trophic Diatom Index (TDI) is explained, highlighting some of the statistical and taxonomic problems encountered, and going on to demonstrate how the system works in practice. Most diatom-based pollution indices, including the TDI, use changes in the relative proportions of different taxa to indicate changing environmental conditions. The techniques involved are therefore much simpler than those involved in many studies of phytoplankton, for example, where absolute numbers are required.

The diatoms of Longans lake-vegetation. Fossil diatom flora. [Translation from: Acta Phytogeographica Suecica 36 73-86, 1955. ]

Sediment samples were taken from Lake Langans in Sweden and fossilised diatoms analysed. Sample methods and environmental factors are discussed. Species with a characteristic occurrence are described. The article discusses diatom-thanatocoenoses as indicators of environment.

'The highly nervous system of the English Lakes': aquatic ecosystem sensitivity to external changes, as demonstrated by diatoms.

The author reviews the stratigraphic diatom profile of Cumbrian lakes since the last glaciation. Knowledge of both present and previous interglacials suggests that a natural cycle of change is imposed on all lakes. The nature of inwashed material is dependant on climatic and natural soilcycles and this affects the water quality and sensitive aquatic biota. Anthropogenic effects are superimposed upon this with forest clearance and pollution. Whilst some Cumbrian diatom profiles extend over the entire post glacial, others cover only detailed sections relating to particular problems. Causes and effect of recent changes in lakes can be studied using indicator species but palaeocology contributes greatly to understanding of long term changes.

Monoculture of marine planktonic diatoms and the effects of different media on their growth and survival

Diatoms are the preferred live food of the protozoea stages of prawns. Due to seasonal variations, it is difficult to obtain continuous supply of diatoms and other algae throughout the year. Therefore mass culture of diatoms is necessary. At attempt was made to culture planktonic diatoms and to study the effect of Allen and Nelson media, Simon media, Vitamin B12 and treated sewage water media on their growth and survival. Navicula sp showed better growth in Allen and Nelson media, Coscinodiscus and Chaetoceros grew better in Simon media while Navicula sp and Coscinodiscus sp showed better growth in the combination of Allen and Nelson + Vitamin B12.

Some planktonic diatoms from the Indian Ocean

Collections of phytoplankton were made by Mr. Durairatnamat various stations between latitude 5°S and 25°S and longitude 78° E and 101° E (Fig. 1) from December 1962 to January 1963 during a cruise of the research vessel "Umitaka Maru" belonging to the Tokyo University of Fisheries. This vessel was engaged in work in connection with the International Indian Ocean Expedition (I.I.O.E.). The collections made from these stations (T.G.) were examined at the Fisheries Research Station, Colombo, for the various diatoms present and the findings are reported in this paper.

Identification and culture of common diatoms as possible feed for Penaeus monodon

Diatoms were collected from Buyuan Bay, and from the hatchery tanks at Tigbauan, to determine the commonly occurring species, the feasibility of culturing these species, and the potential of these selected species as food for larval P. monodon. The commonly occurring diatoms were identified as Chaetoceros calcitrans, Navicula grimmei, Nitzchia seriata, Nitzchia closterium and Amphiprora sp. These diatoms were isolated and unialgal cultures prepared. Protein content analysis using the micro-Kjildahl method gave the following result: C. calcitrans, 11 . 78%; Nitzchia seriata, 25%; Nitzchia closterium, 30 . 5%; Navicula grimmei, 9 . 06% and Amphiprora sp. 8 . 96%. Feeding experiments were conducted to determine acceptability of the different diatom species and percentage survival of larval stages Z SUB-1 -M SUB-2 . Larvae were placed in 4-l capacity plastic containers with a stocking density of 10/l. The results of several feeding trials using the different mass-produced diatoms are summarized. From the data gathered, C. calcitrans appears to be the most promising candidate as feed for zoea and mysis stages of P. monodon. The average percentage survival of C. calcitrans was 63 . 76% for the 3 trials, and as high as 82 . 22% in the third trial. Comparatively high percentage survival of larvae was also recorded when Nitzchia seriata (48 . 17%) and Nitzchia closterium (67 . 6%) were given as feed, while both Amphiprora sp. and Navicula grimmei gave 0% survival. The poor results with Amphiprora sp. and Navicula grimmei may be due to their low protein content (8 . 96% and 9 . 06%, respectively) and the inability of the larvae to ingest them. Navicula and Amphiprora were observed to cling to the appendages of the larvae and to settle down in the medium making them unavailable to the larvae. Low survival was also noted when frozen C. calcitrans was used (14 . 25%). This may be due partly to the effect of the floculating agent (ALSO SUB-4 . 25 g/l) used in concentrating the diatoms. When protein contents of C. calcitrans, N. seriata and N. closterium are compared, the 2 Nitzchia species have relatively higher protein contents than C. calcitrans and, therefore, could be the more desirable feed candidates. However, few feeding trials were made using Nitzchia so that additional investigations will have to be done on this aspect.

Report of the 2004 Workshop on In Situ Iron Enrichment Experiments in the Eastern and Western Subarctic Pacific

Foreword 1. BACKGROUND AND OBJECTIVES (pdf, 0.1 Mb) 2. 2004 WORKSHOP SUMMARY (pdf, < 0.1 Mb) 2.1. What have we learned from the enrichment experiments? 2.2 What are the outstanding questions? 2.3 Recommendations for SEEDS-II 3. EXTENDED ABSTRACTS OF THE 2004 WORKSHOP 3.1 Synthesis of the Iron Enrichment Experiments: SEEDS and SERIES (pdf, 0.5 Mb) Iron fertilization experiment in the western subarctic Pacific (SEEDS) by Atsushi Tsuda The response of N and Si to iron enrichment in the Northeast Pacific Ocean: Results from SERIES by David Timothy, C.S. Wong, Yukihiro Nojiri, Frank A. Whitney, W. Keith Johnson and Janet Barwell-Clarke 3.2 Biological and Physiological Responses (pdf, 0.2 Mb) Zooplankton responses during SEEDS by Hiroaki Saito Phytoplankton community response to iron and temperature gradient in the NW and NE subarctic Pacific Ocean by Isao Kudo, Yoshifumi Noiri, Jun Nishioka, Hiroshi Kiyosawa and Atsushi Tsuda SERIES: Copepod grazing on diatoms by Frank A. Whitney, Moira Galbraith, Janet Barwell-Clarke and Akash Sastri The Southern Ocean Iron Enrichment Experiment: The nitrogen uptake response by William P. Cochlan and Raphael M. Kudela 3.3 Biogeochemical Responses (pdf, 0.5 Mb) What have we learned regarding iron biogeochemistry from iron enrichment experiments? by Jun Nishioka, Shigenobu Takeda and W. Keith Johnson Iron dynamics and temporal changes of iron speciation in SERIES by W. Keith Johnson, C.S. Wong, Nes Sutherland and Jun Nishioka Dissolved organic matter dynamics during SEEDS and SERIES experiments by Takeshi Yoshimura and Hiroshi Ogawa Formation of transparent exopolymer particles during the in-situ iron enrichment experiment in the western subarctic Pacific (SEEDS) by Shigenobu Takeda, Neelam Ramaiah, Ken Furuya and Takeshi Yoshimura Atmospheric measurement by Mitsuo Uematsu 3.4 Prediction from Models (pdf, 0.3 Mb) Modelling iron limitation in the North Pacific by Kenneth L. Denman and M. Angelica Peña A proposed model of the SERIES iron fertilization patch by Debby Ianson, Christoph Voelker and Kenneth L. Denman 4. LIST OF PARTICIPANTS FOR THE 2004 WORKSHOP (pdf, < 0.1 Mb) APPENDIX 1 Report of the 2000 Planning Workshop on Designing the Iron Fertilization Experiment in the Subarctic Pacific (pdf, 1 Mb) APPENDIX 2 Terms of Reference for the Advisory Panel on Iron fertilization experiment in the subarctic Pacific Ocean (pdf, < 0.1 Mb) APPENDIX 3 Historical List of Advisory Panel Members on Iron fertilization experiment in the subarctic Pacific Ocean (pdf, < 0.1 Mb) APPENDIX 4 IFEP-AP Annual Reports (pdf, 0.1 Mb) APPENDIX 5 PICES Press Articles (pdf, 0.6 Mb) (194 page document)

Lithology and palynology of cave floor sediment cores from Wakulla Spring, Wakulla County, Florida

Five short bottom sediment cores taken in Wakulla Spring Wakulla County, Florida, were described lithologically and sampled for palynological study. Four of the cores were recoveredfrom sediments at the spring cave entrance (130 feet water depth). One core was taken in a fossil vertebrate bone bed, 280 feet distance into the main spring cave at a water depth of 240 feet. Sediments in the cores are composed of alternating intervals of quartz sand and calcilitite, containing freshwater diatoms, freshwater mollusk shells and plant remains. The predominant pollen present in all cores consists of a periporate variety typical of the herb families Chenopodiaceae and Amaranthaceae. Arboreal flora, typical of the area surrounding the spring today, represent a very low percentage of thle pollen assemblage in the cores. Clustered Chenopod-Amaranth type pollen observed in one core suggest minimal transport prior to deposition, and indicate that the bottom sediments in the cave may be essentially In situ. An absence of exotic flora suggests a Quaternary age for the sediments. (PDF contains 11 pages.)

Biological survey of the Panama Canal Zone (1912)

At first it was intended to confine the collections to the Canal Zone proper, but as the faunal and floral areas extended to the north and south of this region, it was decided to carry the work into the Republic of Panama, a step which met with the hearty approval of that Republic. The work accomplished has been very valuable to science, including collections and observations of vertebrate animals, land and fresh water mollusks, and plants, including flowering plants, grasses and ferns. Special attention will be given during the coming season to vertebrate animals, insects, crustaceans, rotifers and other minute freshwater animals, and also to the microscopic plants known as diatoms. Includes appendix of papers that resulted.

A quantitative analysis of the phytoplankton of the Gulf of Panama: I. Results of the regional phytoplankton surveys during July and November 1957 and March 1958

ENGLISH: 1. Quantitative phytoplankton samples were collected by the Inter-American Tropical Tuna Commission at the surface and ten meters in the Gulf of Panama, as follows: a) 18-21 March, 1958 (31 stations)-during the height of the upwelling season, b) 10-12 July, 1957 (10 stations)-during the transition to the rainy season at a time when mild upwelling winds reappear, c) 7-8 November, 1957 (15 stations)-during the height of the rainy season. 2. Maximum phytoplankton populations occurred during the upwelling season, followed by a considerable decline during July, and a further Subsidence during November. 3. A remarkable regional uniformity in species composition was observed during the surveys despite regional differences in growth conditions. Diatoms overwhelmingly dominated the communities. 4. During all surveys, the innermost regions, generally north of 8°30'N, were the most productive. The least productive areas were in the offing of San Miguel Bay and Parita Bay, suggesting that nutrient accretion via runoff is inadequate to sustain sizeable autotrophic plant populations in those regions. 5. During all surveys, phytoplankton growth appeared to be limited by nutrient availability. 6. During all surveys, phytoplankton growth appeared to be related to depth of the water column. 7. Although below average rainfall contributed to unusually favorable growth conditions (reduced stability, increased transparency and, presumably, nutrient reserves) during the November survey relative to November 1955 and 1956 at 8°45'N, 79°23'W, the anticipated heightened phytoplankton response was not observed. 8. During the November survey, the local diatom responses and their regional fluctuations could be satisfactorily related to the accompanying surface salinity conditions. However, this correspondence is undoubtedly attributable to factors associated with the observed salinity levels, probably nutrients, rather than salinity directly. 9. Unusually warm conditions occurred during the March survey, attributable to considerably weaker upwelling winds than normally occurring then, which contributed to a considerably lower standing crop and a retardation in succession of three to five weeks relative to that observed during 1955-1957 at 8°45'N, 79°23'W in the Gulf of Panama. 10. During the March survey, a well defined inverse relationship existed between mean temperature and mean diatom abundance in the upper ten meters, and between transparency and mean diatom abundance. A direct relationship occurred between surface salinity and mean diatom abundance in the upper ten meters. These relationships are interpreted to indicate that diatom abundance primarily reflected the nutrient concentrations associated with a given upwelling intensity, rather than describing casual relationships. 11. The survey results indicate that the phytoplankton dynamics observed at 8°45'N, 79°23'W from November, 1954 through May, 1957 are generally representative of the Gulf of Panama. 12. The following new forms, to be described in a later publication, were observed during the surveys: Actinoptychus undulatus f. catenata n.f., Asterionella japonica f. tropicum n.f., Leptocylindrus maximus n. sp., Skeletonema costatum f. tropicum n.f. SPANISH: 1. La Comisión Interamericana del Atun Tropical recolectó en el Golfo de Panama muestras cuantitativas de fitoplancton en la superficie y a los diez metros, como sigue: a) Del 18 al 21 de marzo de 1958 (31 estaciones)-durante el maximum de la estación de afloramiento. b) Del 10 al 12 de julio de 1957 (10 estaciones)-durante la epóca de transición a la estación lluviosa cuando reaparecen los vientos ligeros que causan el afloramiento. c) Del 7 al 8 de noviembre de 1957 (15 estaciones)-durante el maximum de la estación lluviosa. 2. Las poblaciones maximas de fitoplancton aparecieron durante la estación de afloramiento, seguido por una considerable disminución durante el mes de julio y una calma durante noviembre. 3. Durante la investigación se observó una remarcable uniformidad regional en la composición de las especies a pesar de las diferencias regionales en las condiciones de crecimiento. Las diatomeas predominaban en gran numero en las comunidades. 4. Durante todas las investigaciones, las regiones mas cerca de la costa, generalmente al norte de los 8°30'N, eran las mas productivas. Las areas menos productivas fueron las mar afuera de las Bahias de San Miguel y Parita, lo que sugiere que el aumento en las sales nutritivas causado por las escorrentias es inadecuado para sostener poblaciones grandes de plantas autotróficas en estas regiones. 5. Durante todas las investigaciones, el crecimiento del fitoplancton parecio estar limitado por la disponibilidad de las. sales nutritivas. 6. Durante todas las investigaciones el crecimiento del fitoplancton parecio estar relacionado con la profundidad de la columna de agua. 7. Aunque las precipitacion por debajo del promedio normal contribuyo a condiciones desusadamente favorables de crecimiento (estabilidad reducida, aumento de la transparencia y, presumiblemente, de la reserva de sales nutritivas) durante la investigación de noviembre en relación a noviembre de 1955 y de 1956 en los 8°45'N, 79°23'W, no se observo-la alta reacción de fitoplancton que se esperaba. 8. Durante la investigación de noviembre, las reacciones locales de las diatomeas y sus fluctuaciones regionales pudieron relacionarse en forma satisfactoria con condiciones asociadas con la salinidad de la superficie. Sin embargo, esta correspondencia puede atribuirse sin duda a factores asociados con los niveles observados de salinidad, probablemente con las sales nutritivas, en lugar de directamente con la salinidad. 9. Condiciones calurosas no comunes ocurrieron durante la investigación de marzo, las que pueden atribuirse a que los vientos que ocasionan el afloramiento fueran mas debiles que los normales, lo que contribuyó a que la cosecha estable fuera considerablemente mas baja y a la demora de tres a cinco semanas en la sucecion relativa a la que se observó durante 1955-1957 en los 8°45'N, 8°23'W, en el Golfo de Panama. 10. Durante la investigación de marzo, existió una relación inversa bien definida entre la temperatura y la abundancia media de las diatomeas en los diez metros superiores, y entre la transparencia y la abundancia media de las diatomeas. Una relación directa ocurrio entre la salinidad de superficie y la abundancia media de las diatomeas en los diez metros superiores. Estas relaciones se interpretan como indicadoras de que la abundancia de diatomeas refleja primeramente las concentraciones de las sales nutritivas asociadas con una intensidad de afloramiento dada, en lugar de describir relaciones causales. 11. Los resultados de la investigacion indican que la dinamica del fitoplancton observada en los 8°45'N, 79°23'W, desde noviembre de 1954 a mayo de 1957, es generalmente representativa del Golfo de Panama. 12. Durante las investigaciones se observaron las siguientes formas nuevas, las que seran descritas en una publicación posterior: Actinoptychus undulatus f. catenata n.f., Asterionella japonica f. tropicum n.f., Leptocylindrus maximus n. sp., Skeletonema costatum f. tropicum n.f.

The food and feeding habits of the anchoveta, Cetengraulis mysticetus, in the Gulf of Panama

ENGLISH:The gill rakers of both juvenile and adult anchovetas are long and numerous, with many fine processes which make a very efficient straining apparatus. The stomach is modified into a gizzard. The intestine undergoes heteronomous growth, and attains about eight times the standard length in adults. The stomach contents of 39 samples of juvenile fish and 120 adult fish were examined. Diatoms were the principal food of all the sizes of fish examined, from 29 to 153 millimeters. Silicoflagellates, dinoflagellates, pollen grains, formaniferans, rotifer shells, crustaceans, and eggs, probably of crustaceans, were also found in small amounts. Coscinodiscus, a diatom, was the most important item found in the stomachs of the juvenile fish. No strong differences were observed in the feeding habits of different sizes of juveniles. Even taking into account their smaller size, the juveniles had smaller volumes of material and lesser numbers of organisms in their stomachs than did the adults. The stomachs of the adult fish, unlike those of the juveniles, usually contained considerable quantities of mud. Melosira, Coscinodiscus, and Thalassionema, all diatoms, were the most important organisms found in the stomachs of the adults. The incidence of Melosira was much higher in the stomachs of fish from the areas to the east of the entrance of the Panama Canal than from those to the west. No seasonal differences in the food were observed. The volume of material in the stomachs ranged from almost none to nearly 1.0 milliliter, with an average of a little more than 0.2 milliliter. Twenty-six bottom samples were examined; the organisms found corresponded very closely to those encountered in the stomachs of the adult fish. It is concluded that the juvenile anchovetas are chiefly or entirely filter feeders of the pelagic zone. The adults, however, are mostly iliophagous feeders, but possibly do some feeding upon plankton as well. SPANISH:Las branquispinas de las anchovetas, tanto en las juveniles como en las adultas, son largas y numerosas, can varias protuberancias finas que hacen de ellas un aparato filtrador muy eficiente. El estómago está modificado en una molleja. El intestino está sometido a un crecimiento heterónomo, llega a alcanzar unas oeho veces la longitud estandar en las adultas. Fué examinado el contenido estomacal de 39 ,muestras de peces juveniles y de 120 adultos. Las diatomeas fueron el alimento principal de todos los peces que fueron examinados cuyo tamaño varió entre los 29 y 153 milimetros. Se encontraron también en cantidades silicoflagelados, dinoflagelados, granos de polen, foraminíferos, conchas de rotiferos, crustáceos y huevos, probablemente de crustáceos. Coscinodiscus, una diatomea, fué el alimento más importante encontrado en los estómagos de los peces juveniles. No se observaron mayores diferencias en los hábitos de alimentación en los juveniles de diferentes tamaños. Aún tomando en cuenta su tamaño menor, los juveniles tenian volúmenes más pequeños de material y un número menor de organismos en sus estómagos que los adultos. Los estómagos de los peces adultos, diferentes a los de los juveniles, contenían por lo general considerables cantidades de fango. Melosira, Coscinodiscus, y Thalassionema, todas ellas diatomeas, fueron los organismos más importantes encontrados en los estómagos de los adultos. La contribuciónde Melosira fué mucho más alta en los estómagos de los peces procedentes de las áreas al este de la entrada del Canal de Panamá que la de aquellos provenientes del oeste. No se observaron diferencias estacionales en la alimentacion. El volúmen de material en los estómagos varió de casi cero a cerca de 1.0 mililitros, con un promedio de un poco mas de 0.2 mili1itros. Se examinaron 26 muestras de fonda; los organismos encontrados correspondieron muy cercanamente a los hallados en los estómagos de los peces adultos. Se ha llegado a la conclusión de que las anchovetas juveniles son principalmente ó enteramente filtradoras de alimentos de la zona pelágica. Las adultas, sin embargo, son en su mayoria iliófagas, pero posiblemente se alimentan también de plancton.

The occurrence of two new plankton diatom populations in the English Lake District: Aulacoseira islandica

Samples of planktonic algae from major lakes in the English Lake District have been investigated over many years as part of a long-term monitoring programme set up by the Freshwater Biological Association and continued by the Institute For Ecology. Some lakes have been sampled regularly for long periods, others less frequently or only occasionally. Windermere north and south basins, and the neighbouring lakes Blelham Tarn and Esthwaite Water, have been under observation, mostly on a weekly basis, since the programme began in 1945. During this time diatoms have played a significant role in the population dynamics of these lakes.

A review of current biological and recent environmental research on Lake Baikal from a British perspective

In 1990, "BICER" or the Baikal International Centre for Ecological Research was created to foster collaborative research on Lake Baikal. The British effort in BICER was initiated and is administered by the Royal Society, London. Much of the on-going research effort is now focussed on environmental change, as there is increasing concern about recent changes in the lake's unique ecosystem that could be linked with the effects of water pollution from catchment effluents. Monitoring studies of the phytoplankton in Lake Baikal's southern basin indicate that several species have increased in abundance since the mid-70's. Diatoms in Lake Baikal sediments are also being studied.

Chrysosphaerella and the phytoplankton of the Little Sea, Dorset

The Little Sea is an 80-acre, shallow freshwater lake formed about a hundred years ago by sand-dunes cutting off a sea-inlet at Studland Bay, near Swanage. This work presents a general survey of the phytoplankton in the lake from October 1990 to December 1993. Many species were present throughout the year; others showed seasonal variations. Numerically, the diatoms, Monoraphidium and sometimes Rhodomonas, were the main constituents of the phytoplankton. One species of alga in the lake of particular interest is Chrysosphaerella longispina Lauterb. which, up to 1991, had only been recorded from five localities in Britain.

Habitat use by 0+ cyprinid fish in the River Great Ouse, East Anglia

This study was designed to examine the habitat use of several species of 0+ cyprinid in the regulated River Great Ouse and to determine the reasons for specific habitat use. In general, all fish species were found associated with the marginal zone, with little diel variation. Use of shallow habitats in the presence of macrophytes correlated well with the distribution of zooplankton in the river channel, the preferred food source of 0+ cyprinids. During the early to late larval phase, all species fed upon rotifers and diatoms. Cladocera, particularly Alona spp. and Chydorus spp., and early instar larvae of Chironomidae, then became prevalent in the diet along with small numbers of Copepoda. Models were developed to determine habitat availability over a range of discharges, using the physical habitat simulation (PHABSIM) component of the Instream Flow Incremental Methodology (IFIM). The results of this analysis revealed that habitat suitable for 0+ fishes comprised a relatively small percentage of the main channel and generally decreased with discharge.