Endocrine disruption in fish: An assessment of recent research and results

This report provides an assessment of recent investigations into endocrine disruption in fresh and saltwater species of fish. Most work to date has concen-trated on reproductive endocrine disruption. Laboratory studies have shown a variety of synthetic and natural chemicals including certain industrial intermediates, PAHs, PCBs, pesticides, dioxins, trace elements and plant sterols can interfere with the endocrine system in fish. The potency of most of these chemicals, however, is typically hundreds to thousands of times less than that of endog-enous hormones. Evidence of environmental endocrine disruption ranges from the presence of female egg proteins in males and reduced levels of endogenous hormones in both males and females, to gonadal histopathologies and intersex (presence of ovotestes) fish. Overt endocrine disruption in fish does not appear to be a ubiquitous environmental phenomenon, but rather more likely to occur near sewage treatment plants, pulp and paper mills, and in areas of high organic chemical contamination. However, more wide-spread endocrine disruption can occur in rivers with smaller flows and correspondingly large or numerous wastewater inputs. Some of the most severe examples of endocrine disruption in fish have been found adjacent to sewage treatment plants. Effects are thought to be caused prima-rily by natural and synthetic estrogens and to a lesser extent by the degradation products of alkylphenol poly-ethoxylate surfactants. Effects found in fish near pulp and paper mills include reduced levels of estrogens and androgens as well as masculinization of females, and has been linked to the presence of β-sitosterol, a plant sterol. Effects seen in areas of heavy industrial activity typically include depressed levels of estrogens and androgens as well as reduced gonadal growth, and may be linked to the presence of PAHs, PCBs, and possibly dioxins. At this time, however, there is no clear indication that large populations of fish are being seriously impacted as a result of endocrine disruption, although additional work is needed to address this possibility. (PDF contains 63 pages)

Vertical and seasonal distribution of phytoplankton in the Shen Reservoir Plateau State, Nigeria

The phytoplankton distribution of the Shen Reservoir, Bukuru in the Jos Plateau, Nigeria was monitored at 6 depths. Higher floral abundance occurred within the upper 00-03 meters with highest values at the first 1 meter. Bacillariophyceae and Dinophyceae recorded higher values in March-April with lower values in July and January respectively. Phytoplankton were most abundant in the rainy season. Secchi disc transparency was lowest in the peak of the rainy season (July) due to higher levels of suspended matter resulting from the increased run-off from surrounding farmlands of allochthonous materials as well as higher levels of phytoplankton population arising from the former factor. The low water temperature of December/January 15 degree C plus or minus 2 degree C might have depressed growth among the major groups of plankters but enhanced rapid multiplication of the Chlorophyta, Trachelomonas which showed a bloom at this season

Growth response and survival of F1 hybrid fry of Heterobranchus longifilis and Clarias gariepinus reared in glass aquaria and plastic basins

The growth response of F1 hybrid fry of female Heterobranchus longifilis and male Clarias gariepinus were investigated under laboratory conditions in glass aquaria glass tanks and plastic basins. The larvae were produced artificially after inducement with Ovarptim. The hatching percentage was very high. Weekly mean length and weight were monitored for 6 weeks. The average length increase was higher in aquaria glass tanks than in plastic basins. However, there were depressed and irregular weight increases in both types of rearing troughs while significant weight increase (P<0.05) was recorded at week 6 in the plastic basin. Generally, the growth rate and survival in both containers were not significantly different

Changes in Fish Communities in the Upper Patuxent River from 1966 to 1977

Ten year comparison of fish survey's with respect to diversity evenness and composition of fish communities. The upper Patuxent River was divided into Piedmont Plateau and Coastal Plain regions, not only for geographical purposes, but also because of the clustering of sewage treatment plants in the Coastal Plain region. In the Piedmont Plateau region, the fish species diversity changed very little from 1966 to 1977 ( Little Patuxent -- 2.82 to 2.66; Middle Patuxent -- 2.86 to 2.83; and main stem -- 2.46 to 2.63), except in a section of Little Patuxent River at and below the City of Columbia where the species diversity index showed a significant reduction from 2.97 to 1.99, and in a section of the main stem Patuxent River immediately downstream from the Brighton Dam of the Triadelphia Reservoir where the index increased significantly from 1.66 to 3.20. In the Coastal Plain region, a significant reduction in the fish species diversity index occurred between 1966 and 1977 below the two sewage treatment plant outfalls : Savage -- 2.69 to 0 and Patuxent-Crofton -- 3.06 to 1.33. Also, the substantial reduction in the species diversity index which had already occurred in 1966 below the six other plant: outfalls of Fort Meade No. I, Fort Meade No. 2, Maryland House of Correction, Maryland City , Parkway and Bowie, remained depressed in 1977. On the other hand, below the Horsepen Sewage Treatment Plant (a tertiary plant practicing dechlorination) the species diversity index increased from 1.91 to 2.8. (PDF contains 48 pages)

Effects of stress on the immune system of fish

The effects of stress on the immune system of various fish species including dab Limanda limanda, flounder Platichthys flesus, sea bass Dicentrarchus labrax and gobies Zosterisessor ophiocephalus, were investigated from laboratory and field experiments, using various assays to measure immunocompetence, correlated with histological and ultrastructural observations. Modulation of the immune system was demonstrated at tissue, cellular and biochemical levels following exposure to various stressors. The spleen somatic index was depressed in dab stressed in the laboratory and gobies collected from polluted sites in the Venice Lagoon. Differential blood cell counts consistently showed an increase in phagocytes and decrease in thrombocytes in fish exposed to various stressors. Phagocytic activity from spleen and kidney adherent cells was stimulated in dab stressed by transportation but depressed in fish exposed to chemical pollutants. Respiratory burst activity in phagocytic cells was also stimulated in stressed dab but depressed in sea bass exposed to cadmium. The results are discussed in relation to current concepts on stress in fish and the regulation of the immune system.

Species management in aquatic habitats WRc RD Interim 1997

This is the Species management in aquatic Habitats WRc Interim 1997 document produced by the Environment Agency in 1997. This document reports progress on R&D Project 640, which aims to provide information on species of conservation value of particular relevance to the Environment Agency, in relation to its activities affecting aquatic environments. A range of stand-alone outputs is being produced, comprising Species Action Plans, practical management guidelines for Agency staff and third parties, and various research outputs to improve the knowledge base on the status and ecological requirements of priority species. The species of conservation values are: water shrew, daubenton’s bat, Kingfisher, yellow wagtail, Grey wagtail, sand martin, reed bunting, dipper, marsh warbler, great crested new, spined loach, brook lamprey, river lamprey, sea lamprey, shining rams-horn snail, little whirlpool rams-horn snail, depressed river mussel, a freshwater pea mussel, native crayfish, and triangular club-rush. The process of species selection was altered during the course of the project by the report on biodiversity by the UK Biodiversity Steering Group (1995). Whilst still including species that were not particularly endangered but were greatly influenced by the activities of the Agency, the project addressed species on the ‘short’ and ‘middle’ priority lists of the Biodiversity report, particularly those for which the Agency had specific responsibilities.

Fish consumption by harbor seals (Phoca vitulina) in the San Juan Islands, Washington

The harbor seal (Phoca vitulina) is a large-bodied and abundant predator in the Salish Sea ecosystem, and its population has recovered since the 1970s after passage of the Marine Mammal Protection Act and the cessation of bounties. Little is known about how this large predator population may affect the recovery of fish stocks in the Salish Sea, where candidate marine protected areas are being proposed. We used a bioenergetics model to calculate baseline consumption rates in the San Juan Islands, Washington. Salmonids (Oncorhynchus spp.) and herring (Clupeidae) were the 2 most energetically important prey groups for biomass consumed by harbor seals. Estimated consumption of salmonids was 783 (±380 standard deviation [SD]) metric tons (t) in the breeding season and 675 (±388 SD t in the nonbreeding season. Estimated consumption of herring was 646 (±303 SD) t in the breeding season and 2151 (±706 SD) t in the nonbreeding season. Rockfish, a depressed fish stock currently in need of population recovery, composed one of the minor prey groups consumed by harbor seals (84 [±26 SD] t in the nonbreeding season). The variables of seal body mass and proportion of prey in seal diet explained >80% of the total variation in model outputs. Prey groups, such as rockfish, that are targeted for recovery may still be affected by even low levels of predation. This study highlights the importance of salmonids and herring for the seal population and provides a framework for refining consumption estimates and their confidence intervals with future data.

Snow accumulation time series and their climatic interpretation

EXTRACT (SEE PDF FOR FULL ABSTRACT): Several snow accumulation time series derived from ice cores and extending over 3 to 5 centuries are examined for spatial and temporal climatic information. ... A significant observation is the widespread depression of net snow accumulation during the latter part of the "Little Ice Age". This initially suggests sea surface temperatures were significantly depressed during the same period. However, prior to this, the available core records indicate generally higher than average precipitation rates. This also implies that influences such as shifted storm tracks or a dustier atmosphere may have been involved. Without additional spatial data coverage, these observations should properly be studied using a coupled (global) ocean/atmosphere GCM.

A new deep-sea crab, genus Chaceon, from the Arabian Sea (Crustacea, Decapoda, Geryonidae)

The sixth nominal species of Chaceon to be recorded from the western Indian Ocean is named from a specimen collected off Somalia in 504-506 meters. Chaceon somaliensis, n. sp., resembles C. macphersoni (Manning and Holthuis, 1988) and differs from the other four species known from the area in having the dactylus of the walking legs dorsoventrally depressed. It differs from C. macphersoni in having slenderer legs, a smoother body, and a much deeper, evenly curved orbit.

Field application of anabolic steroids in carp seed production. Pt. 1. Rearing of fry to fingerling stage

Ethylestrenol (17β Hydroxy-17alpha-ethyl-estr-4-en-3-one) and Stanozolal (17β-Hydroxy-17alpha-methyl 1-5 alpha-androstano-(3,2-C)-pyrazole), both synthetic androgenic steroids, were fed via diet at 3ppm to the fry of catla, rohu and silver carp which were reared up to fingerling stage over a period of 167-172 days in earthen ponds. Ethylestrenol enhanced growth in silver carp and rohu but retarded growth in catla. Stanozolal depressed growth in all the 3 species. Length-weight relationship for these fry had been worked out and the relative condition factor in all the cases was very close to or slightly above 1.0.

On statistical comparison of efficiencies of fishing gear

The distribution of fish caught by experimental gill nets has been found to be in the Poisson or Negative binomial form. Using this information, application of Chi-square test as suggested by Mood et al. (1974) has been illustrated, for comparing the efficiencies of gill nets. This test provides an alternative to Anova F-test especially in the context of significance of non-additivity for the two-way model. Based on the present work and the findings by Nair (1982) and Nair & Alagaraja (1982, 1984) an outline approach for statistical comparison of the efficiencies of fishing gear is presented.

Notes on the induced maturation and spawning in four-month-old Penaeus monodon Fabricius by eyestalk ablation

The ablation technique consisted of making an incision across the eyeball to allow free flow of fluids while holding the prawn under water, squeezing the eyeball contents outwards, and pinching hard the eyestalk tissue. The cut area heals completely in about a week; no application of antibiotics is necessary. Spent spawners were tagged with thin brass rings (Rodriguez, 1976) around the unablated eyestalk for a separate experiment on rematuration. Two spawning yielding approximately 277,000 eggs were obtained three weeks after ablation, followed four days later by two more spawnings with 160,000 eggs; all four spawners weighed more than 100 g. With a hatching rate of 98% and 78% for the first and second batch, respectively, the spawnings produced viable nauplii. Water temperatures as low as 23 degree C due to a delayed cold spell in March depressed molting; weakened larvae had to be discharged at the mysis stage. Although ovarian development continued, no further spawnings were obtained due mainly to the onset of bacterial and fungal disease. Infection is initiated in injured portions of the exoskeleton, sometimes penetrating right through the muscles to the ovarian tissues. The non-flowthrough conditions and mussel meat feeding led to fouling of the culture water resulting in consecutive mortalities caused by disease. Female P.monodon held in maturation pens were ablated at the age of 15 months (Santiago, et al., 1976); they averaged only 16 g body weight after four months growth in ponds. In another experiment, pond-reared P.monodon females ranging from 50 to 80 g were ablated at approximately seven months (Aquacop, 1977). The present results show a minimum age of four months from postlarve that P.monodon is capable of ovarian development and spawning upon ablation. However, maturation is probably affected by size as well as age - the four-month old females weighed an average of 100 g in contrast to the smaller animals in the earlier experiments.

Effects of cage fish culture on water quality and selected biological communities in northern Lake Victoria, Uganda

Growing of fish in cages is currently practiced in Uganda and was first introduced in northern Lake Victoria in 2010. An environment monitoring study was undertaken at Source of the Nile, a private cage fish farm, in Napoleon gulf, northern Lake Victoria. In-situ measurements of key environmental (temperature, dissolved oxygen, pH and conductivity) and biological (algae, zooplankton, macro-benthos) variables were made at three transects: Transect 1- the site with fish cages (WC); transect 2- upstream of the fish cages (USC-control) and Transect 3- downstream of the cages (DSC). Upstream and Downstream sites were located approximately 1.0 km from the fish cages. Environment parameters varied spatially and temporally but were generally within safe ranges for freshwater habitats. Higher concentrations of SRP (0.015-0.112 Mg/L) occurred at USC during February, September and at DSC in November; NO2-N (0.217- 0.042 mg/L) at USC and DSC in February and November; NH4-N (0.0054- 0.065 Mg/L) at WC and DSC in February, May and November. Algal bio-volumes were significantly higher at WC (F (2,780)=4.619; P=0.010). Zooplankton species numbers were consistently lower at WC with a significant difference compared to the control site (P=0.032). Macro-benthos abundance was consistently higher at the site with cages where mollusks and low-oxygen and pollution-tolerant chironomids were the dominant group. Higher algal biomass, concentration of low-oxygen/pollution-tolerant macro-benthos and depressed zooplankton diversity at WC suggested impacts from the fish cages on aquatic biota.

Study ectoparasite infestation of 10 common imported freshwater ornamental fish species

In the present study, ectoparasite infestation of common freshwater ornamental fish species imported into Iran including: Dwarf gourami (Colisa lalia), Angelfish (Pterophyllum scalare), Oscar (Astronatus ocellatus), Red eye Tetra ( Moenkhansia Sanctaefilomenae), Barb( Capoeta tetrazona), Arowana (Osteoglossum bicirrhosum), Goldfish(Carassius auratus), Red fin Shark (Lebeo elythrurus, Catfish (Hypostomus plecostomus) and Pangasius sutchi ( Pangasius hypophthalmus) from May until April was investigated.A total of 6 specimens sample of each fish species) randomly were obtained and taken alive to the lab. After observing gill and skin wet smear under the microscope , gill was dissected and examined carefully .Photo and films were taken from the isolated parasites and parasite identification was performed according to Yamaguti AP1), Bychowsky (■Y).From a total of examined fishes 1\ ► sample (YY.V.) were parasitized .Parasite groups that were observed consisting ciliated protozoan myxosporidian , monogenean Digenean metacercaria and crustacean .The only non-parasitized fish was Barb. Monogenea Trianchoratus sp. , Cleidodiscus sp. , Ancylodiscoides sp. , Thaparocleidus sp. , Centrocestus formosanus metacercaria and Myxobolus longisporus report for the first time in ornamental fish which imported from Southeast of Asia to Iran. According to the results, it seems that severe quarantine and sanitary rules are necessary.