Protocols for conducting dolphin capture-release health assessment studies

Marine mammals, such as dolphins, can serve as key indicator species in coastal areas by reflecting the effects of natural and anthropogenic stressors. As such they are often considered sentinels of environmental and ecosystem health (Bossart 2006; Wells et al. 2004; Fair and Becker 2000). The bottlenose dolphin is an apex predator and a key component of many estuarine environments in the southeastern United States (Woodward-Clyde Consultants 1994; SCDNR 2005). Health assessments of dolphins are especially critical in areas where populations are depleted, show signs of epidemic disease and/or high mortality and/or where habitat is being altered or impacted by human activities. Recent assessments of environmental conditions in the Indian River Lagoon, Florida (IRL) and the estuarine waters surrounding Charleston, South Carolina (CHS) highlight the need for studies of the health of local bottlenose dolphins. While the condition of southeastern estuaries was rated as fair in the National Coastal Condition Report (U.S. EPA 2001), it was noted that the IRL was characterized by poorer than expected benthic communities, significant sediment toxicity and increased nutrient concentrations. Similarly, portions of the CHS estuary have sediment concentrations of aliphatic aromatic hydrocarbons, select inorganic metals, and some persistent pesticides far in excess of reported bioeffect levels (Hyland et al. 1998). Long-term trends in water quality monitoring and recent scientific research suggest that waste load assimilation, non-point source runoff impacts, contaminated sediments, and toxic pollutants are key issues in the CHS estuary system. Several ‘hot spots’ with high levels of heavy metals and organic compounds have been identified (Van Dolah et al. 2004). High concentrations of anthropogenic trace metals, polychlorinated biphenyls (PCB’s) and pesticides have been found in the sediments of Charleston Harbor, as well as the Ashley and Cooper Rivers (Long et al. 1998). Two superfund sites are located within the CHS estuary and the key contaminants of concern associated with these sites are: polycyclic aromatic hydrocarbons (PAH), lead, chromium, copper, arsenic, zinc and dioxin. Concerns related to the overall health of IRL dolphins and dermatologic disease observed in many dolphins in the area (Bossart et al. 2003) initiated an investigation of potential factors which may have impacted dolphin health. From May-August 2001, 35 bottlenose dolphins died in the IRL during an unusual mortality event (MMC 2003). Many of these dolphins were diagnosed with a variety of skin lesions including proliferative ulcerative dermatitis due to protozoa and fungi, dolphin pox and a vesicular dermatopathy of unknown etiology (Bossart et al. 2003). Multiple species from fish to dolphins in the IRL system have exhibited skin lesions of various known and unknown etiologies (Kane et al. 2000; Bossart et al. 2003; Reif et al. 2006). On-going photo-identification (photo-ID) studies have documented skin diseases in IRL dolphins (Mazzoil et al. 2005). In addition, up to 70% of green sea turtles in the IRL exhibit fibropapillomas, with the highest rates of occurrence being seen in turtles from the southern IRL (Hirama 2001).

Influence of nitrogen salts on growth and physiological responses of Rhizophora apiculata Blume in non-aerated water culture

Influence of two different forms of nitrogen on growth and physiological aspects of water-cultured seedlings of Rhizophora apiculata was studied. Of the two forms of nitrogen supplied to the growth medium, ammonium nitrogen was better than nitrate nitrogen by exhibiting increased dry matter production, shoot length, leaf area and also enhanced the contents of carotenoids, chlorophylls and their presence in photosystems and light harvesting protein complex.

Nutrient salts in the United Arab Emirates waters (the Arabian Gulf and the Gulf of Oman)

This paper deals with the levels and distributions of nutrient salts in the United Arab Emirates waters. Water samples were collected bimonthly during 1994-1995 from the marine environment of the United Arab Emirates, which extends more than 800km along the Arabian Gulf and the Gulf of Oman. Concentrations of ammonium, nitrite, nitrate, phosphate, silicate, as well as total concentrations of total dissolved nitrogen, phosphorus, and silicon in the area were scattered in the ranges: (ND-6.32; mean: 0.84 µg-at N/l), ND-3.02; mean: 0.42 µg-at N/l), (ND-10.88; mean: 1.18 µg-at N/1), (ND-4.22; mean: 0.62 µg-at P/l), (1.14-28.80; mean: 6.52 µg-at Si/l), (1.52-39.58; mean: 12.28 µg-at N/l), (0.40-4.98; mean: 1.07 µg-at P/l), and (2.77-44.74; mean: 13.02 Si/l) respectively. Of inorganic nitrogen species, ammonium was the highest in the Arabian Gulf waters and nitrate was the highest at the Gulf of Oman. The dissolved inorganic nitrogen total species, phosphate and silicate amounted to 16.4, 47.6, 56.5% respectively, of the concentrations of nitrogen, phosphorus and silicon in the Arabian Gulf and 22.6, 64.4, 44.9% respectively, in the Gulf of Oman, indicating that more than 80% of nitrogen was present in organic forms. Distributions of nutrient in the two regions were higher in the summer season and lower in the winter season due to the oxidation of organic materials. Regional distributions revealed higher values for nitrite (1.3 times), nitrate (2.8 times), phosphate (2.2 times), total dissolved nitrogen (1.3 times), total dissolved phosphorus (1.6 times), and total dissolved silicon (1.3 times) in the Gulf of Oman compared to the Arabian Gulf, indicating more oligotrophic conditions at the Arabian Gulf Whereas no distinct patterns of distribution were observed in the Arabian Gulf waters, an increase in the seaward direction was measured at the Gulf of Oman. Vertical distributions indicated a general increase with depth in the two regions. The mean ratios for total concentrations of phosphorus, nitrogen, and silicon in the Arabian Gulf (1: 11.6: 12.6) and the Gulf of Oman (1: 10.1: 11.8) were lower than the Redfield ratio.