Trophic State Index, morphoedaphic index and fish yield prediction in a sub-tropical lake, Manchar (Sindh), Pakistan

Limnological factors of a sub-tropical lake Manchar were studied on seasonal basis. The mean values of various parameters were: transparency, (secchi disc reading): 90.5 cm, Orthophosphate: 0.257 mg/l, TDS: 3310,5 mg/l, Conductivity: 5232 µs/l, Total Chlorophyll (Planktonic): 31.3 µg/l Planktonic biomass: 5466 µg/l. Trophic state index (TSI) was calculated by using Carlson's (1977) equations. Mean TSI for transparency was 61, while for orthophosphate and chlorophyll, it was 82 and 64 respectively. TSI values indicate advanced eutrophic state of Manchar Lake. Morphoedaphic index (MEI) was also calculated on seasonal basis. The mean values were, TDS: 1103, conductivity: 1744, alkalinity: 60, transparency: 29 and biomass (plankton dry weight): 1746. Fish yield prediction for Manchar Lake (Z =3m, mean area=100 km²) was calculated by using MEI values. The results were quite different among various parameters. Conductivity (89.1mt/y), biomass (67.6 mt/y) and TDS (44.6 mt/y) were found to be good predictors of fish yield. Chlorophyll, transparency and alkalinity values gave very low estimate.

Estimating above-ground biomass of Melaleuca quinquenervia in Florida, USA

One hundred and thirty-eight Melaleuca quinquenervia (Cav.) S. T. Blake (broad-leaved paperbark) trees were harvested from six sites in South Florida to formulate regression equations for estimating tree above-ground dry weight.

The effect of spirulina (fresh and dry) on some biological factors in Penaeus semisulcatus larvae

Spirulina is a filamentous cyanobacteria with many applications in food and drug industries, as a food in human, aquaculture, vet and poultry industries… . Semi and mass culture of Spirulina carries out in different countries. This study was carried out in five phases in order to produce this microalga in Iran. The first phase, Spirulina pure stock was imported from Indonesia. After identification of species, it was cultured in laboratory until we took 20 liters of biomass. The semi-mass culture was carried out in green house. Cell concentration and size of Spirulina were recorded during culture daily and their growth rates were calculated. After two weeks, when the size of Spirulina was suitable, biomass of Spirulina was harvested then accumulated Spirulina weighted and dried in 24 hours in laboratory. In order to microbiological study, the samples of Spirulina (dry and fresh) were cultured on blood agar medium and coliforms were counted. The chemical composition of produced Spirulina was measured by standard methods. Fatty acid and amino acid profiles were acquired by GC and HPLC instruments, respectively. The amount of chlorophyll in Spirulina was determined by spectroscopy method. Also astaxanthin pigment as an important carotenoid was measured by HPLC in Spirulina and Penaeus semisulcatus larvae fed on Spirulina. At final phase of this project, larva fed on produced Spirulina (biomass and dry powder) was compared to Z plus, microencapsulated Spirulina (M.C.F) and Chaetoceros algae as control. This experiment was carried from zoa to early post larvae stage then survival and growth rate of larvae were recorded. The growth rate of larvae was evaluated with ANOVA test and survival rate of treatments was assessed by Log Rank (Mantel –Cox) test. Also during larvae stage, two parameters of water such as nitrate and nitrite were measured in zoa, mysis and post larvae stages. The results of this study were shown that colifom counts were 1.85×106 and 92.3×105 coliform per ml in fresh and dry spirulina, respectively. Protein percent of dry spirulina was 50.93 % (dry weight) and the amount of astaxanthin in spirulina and larvae fed on spirulina were 0.21 and 0.01 mg/kg, respectively. The most survival rate of larvae were observed in zoa III (88.8%) with Z plus supplement treatment, in mysis III (76.5%) combination of Z plus and dry spirulina in comparative between treatments. Larvae growth (4.5mm) of control in early post larvae was the best.

Study on the inhibitory effects of native medicinal plants on growth and aflatoxin production by toxigenic Aspergilli isolated from rainbow trout feed

In the present study, natural occurrence of fungi and aflatoxin B1 (AFB1) in pellet feed and feed ingredients used for rainbow trout was investigated with emphasis to Aspergillus section Flavi members and medicinal plants inhibitory to Aspergillus growth and/or AF production. The feed samples were cultured on the standard isolation media including dichloran rosebengal chloramphenicol agar (DRCA) and Aspergillus flavus/parasiticus agar (AFPA) for 2 weeks at 28 °C. Identification of fungal isolates was implemented based on the macro- and microscopic morphological criteria. AFs were detected using high performance liquid chromatography (HPLC). Based on the results obtained, a total of 109 fungal isolates were identified of which Aspergillus was the prominent genus (57.0%), followed by Penicillium (12.84%), Absidia (11.01%) and Pseudallscheria (10.10%). The most frequent Aspergillus species was A. flavus (60.66%) isolated from all the feed ingredients as well as pellet feed. Among 37 A. flavus isolates, 19 (51.35%) were able to produce AFB1 on yeast extract-sucrose (YES) broth in the range of 10.2 to 612.8 [tg/g fungal dry weight. HPLC analyses of trout feed showed that pellet feed and all feed ingredients tested except gluten were contaminated with different levels of AFB1 in the range of 1.83 to 67.35 lig/kg. In order to finding natural inhibitors of fungal growth and/or AF production, essential oils (EOs) and extracts of 49 medicinal plants were studied against an aflatoxin-producing A. parasiticus using a microbioassay technique. The EOs was analyzed by gas chromatography/mass spectrometry (GC/MS). Based on the results obtained, Achillea millefolium sub sp. elborsensis, Ferula gummosa, Mentha spicata, Azadirachta indica, Conium maculatum and Artemisia dracunculus remarkably inhibited A. parasiticus growth without affecting AF production by the fungus. Besides of Thymus vulgaris and Citrus aurantifolia, the EO of Foeniculum vulgare significantly inhibited both fungal growth (-70.0%) and AFs B1 and G1 (-99.0%) production. The EO of Carum carvi and ethyl acetate extract of Platycladus orientalis suppressed AFs B1 and G1 by more than 90.0%, without any obvious effect on fungal growth. The IC50 values of bioactive plants for AFs B1 and G1 were determined in the ranges of 90.6 to 576.2 and 2.8 to 61.9 µg/ml, respectively. Overall, results of the present study indicate the importance of AF contamination of trout feed as a risk factor for fish farming and thus, an urgent necessity for constant monitoring of trout feed for any unacceptable levels of AF contamination. Likewise, antifungal activities of bioactive plants introduced here would be an important contribution to explain the use of these plants as effective antimicrobial candidates to protect feeds from toxigenic fungus growth and subsequent AF contamination.

Measures of Plant Surface-areas for Eurasian Watermilfoil and Water Stargrass

Plant surface areas were measured from samples of two common submersed aquatics with widely diverging morphologies: Eurasian watermilfoil ( Myriophyllum spicatum L.) and water stargrass ( Heteranthera dubia (Jacq.) MacM.). Measures for the highly dissected leaves of Eurasian watermilfoil involved development of a regression equation relating leaf length to direct measures of a subsample of leaf parts. Measures for the simple leaves of the stargrass were sums of measured triangles. Stem surfaces for both species were calculated as measured cylinders. Though the means of the stem length and leaf length were larger for stargrass samples, their mean surface area was 95 cm 2 which was less than the 108 cm 2 recorded for Eurasian watermilfoil samples. Relating surface area to dry weight for the stargrass was straightforward, with 1 mg of dry weight yielding an average 0.678 cm 2 of surface area. Biomass measures for the water milfoil were confounded by the additional weight of epiphytic algae persisting on cleaned samples. The results suggest that a lesstime consuming method for surface area measures of plants with highly dissected leaves and a caveat for using biomass measures to estimate surface area in such plants.

Influence of Sediment Nutrients on Growth of Emergent Hygrophila

Hygrophila ( Hygrophila polysperma (Roxb.) T. Anderson) is a plants which forms serious aquatic weed problems. Both submerged and emergent growth forms occur. Nutritional studies with a controlled release fertilizer and sediments collected from hygrophila-infested areas were conducted with the emergent growth habit to provide insights into growth of this introduced plant. Plant dry weights for experimental 16- week culture periods with low average temperatures were associated with low amounts of hygrophila biomass as compared to culture periods with high average temperatures. Hygrophila cultured in sand rooting media with the controlled release fertilizer produced as much as 20 times more dry weight than plants cultured in sediments only. First-degree linear regression statistics showed hygrophila dry weights were highly related to ammonia nitrogen, magnesium, sodium, and pH values in the sediments. These findings show the close relationship of the emergent growth habit of hygrophila to sediment nutrients. Analyses for certain sediment characteristics may provide an indication of the potential growth that may be expected for weed infestations of this plant. Hygrophila grows year round in south Florida; however, visual observations of canals and other bodies of water indicate that lower amounts of hygrophila plants occur during the cooler months of year than during the summer season. These findings show the seasonal growth of emergent hygrophila occurs with biomass dependent on both sediment nutrients and temperature.

The significance of sedimentation and sediments to phytoplankton growth in drinking-water reservoirs

In the mesotrophic-eutrophic Saidenbach Reservoir in Saxony, the nanoplankton and cyanobacteria have increased at the expense of diatom dominance, due to a doubling of the external phosphorus load in the last 15 years. However, the phosphorus sedimentation flux is still very high (up to 80% of the input), corresponding to more than 2 g m2 d-1 in terms of dry weight. There is a strong correlation between the abundance of diatoms in the euphotic zone and their sedimentation flux (with a delay of about 2 weeks). Only about 25% of the deposited material could be clearly attributed to plankton biomass; the remainder resulted from flocculation and precipitation processes or directly from the inflow of clay minerals. The ash content of the deposited material was high (73%). Thus the sedimentation flux can be considered to operate as an internal water-treatment/oligotrophication process within the lake. The neighbouring Neunzehnhain Reservoir still has a very clear water with a transparency up to 18 m depth. Though the sediment was not much lower than Saidenbach sediment in total phosphorus and total numbers of bacteria, sulphide was always absent and the ratio of Fe 2+ to Fe 3+ was very low in the upper (0- 5 cm) layer. Thus the external and internal phosphorus loads do not attain the critical level necessary to induce a ”phosphorus - phytoplankton” feedback loop.

Dix ans de mesures de biomasses de zooplancton a la station cotière d'Abidjan: 1969-1979

Graphs of variations of zooplankton biomasses expressed as ash-free dry weight (i.e. organic matter) are presented for the 1969-1979 period. The graph of the average year shows: an enrichment season from mid-July till mid-November in which the biomass is 2.3 times higher than the rest of the year and characterized by a slight decrease of the biomass in late August or early September. The warm season is divided into a period of moderate biomass from November till February, a period of moderate biomass from November till February and a period of steady decline of the biomass till the start of the upwelling at the end of June.

Estimation de la production zooplanctonique sur le plateau continental ivoirien

An attempt was made to calculate zooplankton production from weights and settled volumes and from the life cycle of some copepods. Biomass data were recorded during several years from 24 monthly cruises and from a coastal station sampled biweekly. Dry weight data were directly measured or were calculated from the settled volumes using a linear regression. They range, on an average, from 0.965 to 5.56 g m-2 day-1 from the shore line to the edge of the continental shelf. The mean life-span of the cohorts of 12 species of copepods is about 20 days. It is assumed that only 1 spawn occurs per generation-time and that the standing stock is turned-over during the life span of a cohort. The production ranges from 48.2 to 278 mg dry weight m-2 day-1 or 17.9 to 103 mg C m-2 day-1, according to the depth of the studied areas. One third of carnivorous production occurs among the copepods. So, it is assumed that the herbivorous and omnivorous production is about 2/3 of the total zooplanktonic production. This would be a more accurate estimate of secondary production. The standing stock of zooplankton and fishes are in the same order of magnitude; the ratio zooplanktonic production/total fishery is 0.8%.

The relationship of shell dimensions and shell volume to live weight and soft tissue weight in the mangrove clam, Polymesoda erosa (Solander, 1786) from northern Australia

Shell dimensions (length, height, width) and shell volume were evaluated as estimators of growth for Polymesoda erosa in northern Australia. Each parameter was a good estimator when applied to live weight (r2 values of 76-96 percent), but not to soft tissue weight (wet, dry, or ash-free dry weight) (r2 values of 13-32 percent). The b value for shell volume to weight relationship of clams collected during the dry season (June to October) was signifi cantly different than for those collected in the wet season (February to April).

Fish farm technology. 1 - Brackishwater fish culture based on "aquafeed"

A method has been evolved to enhance the production of natural feed in brackish water fish farms by providing substrates for bio-growth ('aquafeed' production) which is a biomass complex consisting of sedentary and associated organisms of plant and animal species. The seasonal fluctuations of the aquafeed production over different substrates ranged as: 787-1830g/coconut leaf (6m²)/45 days, 16.0-072.9g/glass panel (2x10x10cm²)/30 days, 52-230g/nylon mat (2x25x25cm²)/30 days and 18.6-123.1g/wooden block (6x10x10cm²)/30 days. The average dry weight composition of the major components of aquafeed obtained in the present study was sand-silt-clay 40%, protein 22%, carbohydrate (water soluble) 1.8% and fat 3.35% (water content 85%). Mugil cephalus of 1.85 cm reared in a 0.01ha pond and fed on aquafeed attained a size of 23 cm length and 146.73g weight during one year. Survival rate was 54% at a density of 1000/ha. Salinity and temperature of the pond during the culture period ranged between 1.4 and 32.8‰ and 28.1 and 36.5°C respectively.

Study of density, distribution and yield on benthic fauna and brown trout (Salmo trutta fario) feeding in Laar reservoirs

Lar lake, with the international UTM specification of 39S 579680 3976567 & 39S 589930 3976184 is Situated in Lar national Park with an aerial distance of 55 Km of Tehran along Haraz road. The present research is carried out as part of a comprehensives Plan for assessment of bioresearches of Lar lake & the rivers flowing into it. This research includes examination of there benthic Samplings performed in Lar lake and each of the related rivers including Delichaee, Ab-e-sefid , Alarm & Lar (Kamardasht).Tubifex and Chironomus genus were found to have the highest frequencies of occurrence in the lake with %77.117 & %21.823 respectively followed by Chironomidae and Simulidae from the Diptera order which accounted for %72.328 and %13.812 occurrences in four rivers examined in the Study. The benthic biomass at various examined Sites and the average wet weight of the benthic biomass in station No one in the lake Was 17.397g and the figure for the examined site in Alarm was 20.242 g which were the highest level among Other examination stations the index for the abundance of species in Alarm river was greater than the rest of the examined rivers with 12.57. A sum of 354 Pieces of brown trouts was caught in the course of sampling which were closely investigated in terms of their digestive tract Content. It was identified that Daphniidae and Chironomus constituted the bulk of eaten items from the lake with %17.985 and %63.973 respectively. Meanwhile, Chironomidae and Simuladae were the most frequently eanten benthos by the fish with %81.47 and %7.93 respectively.The index for the relative length of gut was recorded at 0.49± 0.08 which is well indicative of the carnivorous diet of the fish.The index for the feeding intensity amounted to 138  83 showing that the one year old fish were of more feeding intensity.The coefficient of condition (K) was estimated at 1.02  0.142 for all the caught fish. The average wet weight of the benthos was 10.348 g per square meter which if extended to 700ha surface area of the lake, the total macrobenthic production in the lake would amount to 72730Kg of wet weight or 6510 Kg of dry weight. Since the Secondary Production of macrobenthos have always been double that of their biomass, it is reasonable to assume that the Secondary Production of macrobenthos amount to 145640 Kg by their wet weight and Since the energy transfer in the food chain of the lake from benthos to fish is 10 percent, the fish production Capacity Coming from benthic resources of the lake (Lar) would be 14.5 MT, half of which (7000-8000MT) could annually be harvested. Further more, the actual fish Production Capacity might exceed the projected level Since Daphnia, Rotifers and Ostracoda which belong to Zooplanktons, play a part in the natural diet of trout. Meanwhile, rivers Play a major role in fish nutrition and the annual fish production in Delichaee river is about 4481.8Kg while the figures for Ab-e-sefid, Alerm and Lar rivers are 2370.7 4848.7 and 2586.2 Kg respectively, that further increase fish Production in the area and every year half of these resources can be exploitable from the river & the lake.Nevertheless, due to ecological & biological importance of rivers and the probability of environmental Pollution, devastation of natural fish habitats & their nursery grounds, Sport fishing is not recommended at all.

Length-weight relationship of benthic bivalves of the Andoni Flats, Niger Delta, Nigeria

The length weight relationship of three benthic bivalves namely, Senilia (= Anadara) senilis (bloody cockle), Tagelus adansonii (knife clam), Tellina nymphalis (soft shell clam) from the Andoni Flats were determined. The bivalves which are of ecological importance were obtained from the intertidal areas of the Andoni Flats. Shell lengths of the bivalves were measured and corresponding dry weight measurements were also taken. The data obtained were then subjected to regression analysis using the FAO-ICLARM Fish Stock Assessment Tools (FiSAT). The length weight relationships obtained from the FiSAT analysis indicated isometric growth for Senilia (= Anadara) senilis, with slope (b) value of 2.942; positive allometric growth for Tagelus adansonii, with a ‘b’ value of 3.395 and negative allometric growth for Tellina nymphalis with ‘b’ value of 2.633. KEYWORDS: bivalves, length-weight, isometric growth, allometric growth, cockle, clam.

Seasonal Growth of Waterhyacinth in the Sacramento/San Joaquin Delta, California

Waterhyacinth ( Eichhornia crassipes (Mart.) Solms), is a serious problem in the Sacramento/San Joaquin Delta, California. There is little published information on its phenology or seasonal growth in this system. Waterhyacinths were sampled at 2 to 3 week intervals from November, 1995 to July, 1997 and the following measurements were made on individual plants: dry weight, height, number of living leaves, number of dead leaves, and the width of the largest lamina. (PDF has 4 pages.)

Estimating the Energy Density of Fish: The Importance of Ontogeny

Ontogenetic patterns in the percent dry weight (%DW) and energy density (joules per gram of wet weight) were studied in the early life stages of the subtropical estuarine and marine gray snapper Lutjanus griseus and the warmtemperate estuarine and marine spotted seatrout Cynoscion nebulosus. The %DW was variable for individuals of both species but increased significantly through larval to juvenile stages (<20% for fish ,50 mm standard length to 20–30% for fish >50 mm). The lipid percentage, which was determined only for gray snapper, was also variable between individuals but showed significant increase with body size. Strong relationships between percent dry weight and energy density were evident for both species; however, the slopes of regressions were significantly lower than in general multispecies models, demonstrating the need for species- and stagespecific energy density data in bioenergetics models.