Growth response of Heterobranchus longifilis (Valenciennes, 1840) fingerlings fed raw and boiled Mucuna cochinchinensis seed meal

Feeding trial was conducted in static water to assess the growth of H. longifilis fingerlings fed different I inclusion levels of mucuna seed meal (MSM). Raw and boiled MSM were used in the diets at 10%, 20%, 30% and 40% inclusion levels and the performance of fish fed these diets was compared with fish fed a fishmeal-based diet which contained 40% protein. All diets were prepared to be isonitrogenous and isocaloric A two by five factorial experiment with three replicates using ten fish of average initial weight 1 .46g was carried out. Daily fish ration of five percent body weight was administered two times for eight weeks. The specific growth rate in diets 1 (control) and 6(10% boiled MSM) were similar and significantly (P<0.05) higher than the other dietary groups. The significantly lower growth performance of fish fed diets containing higher inclusion levels of both raw and boiled MSM might be due to incomplete elimination of the antinutritional factors present in MSM by boiling. Other methods of processing MSM to improve its nutritive value should be investigated. (7 page document)

Cryopreservation of sperm of common carp, Cyprinus carpio and silver barb, Barbonymus gonionotus for genetically improved seed production

Experiments were conducted to develop and standardize the protocols for cryopreservation of sperm of common carp, Cyprinus carpio and also for using the cryopreserved sperm for fertilization of eggs. Nine extender solutions as Alsever's solution, kurokura-1, kurokura-2, urea egg-yolk, egg-yolk citrate, 0.6% glucose, 0.9% NaCl, Ma and Mb, and five cryoprotectants namely ethanol, methanol, dimethylsulfoxide (DMSO), dimethylamine (DMA) and glycerol were tested. The cryoprotectants were mixed at 10% concentration of the extenders (v/v) to make the cryodiluents. Milt and cryodiluents were mixed at a ratio of 1:9 for Alsever's solution, kurokura-1, kurokura-2, 0.6% glucose and 0.9% NaCl, 1:4 for urea egg-yolk, egg-yolk citrate, Ma and Mb. Among the cryodiluents Alsever's solution mixed with either ethanol or methanol was found to be suitable and it produced more than 90% and 80% spermatozoan motility at equilibrium and post-thaw periods, respectively. Kurokura-1 and kurokura-2 when mixed with the same cryoprotectants showed good spermatozoan motility at equilibrium period (80-90%) but the motility was reduced (30-55%) at post-thaw state. Other extenders did not produce acceptable sperm-motility and in some cases the frozen milt became clotted. Different dilution ratios (1:1, 1:2, 1:4, 1:5, 1:7, 1:9, 1:12, 1:15, 1:20) were formulated for obtaining a suitable milt dilution, the dilution ratio of 1: 9 (milt : cryodiluent) demonstrated the highest post-thaw spermatozoan motility (80%) in Alserver's solution. The optimum concentration of cryoprotectants in the cryodiluents was determined, 10% concentration level was found to be effective to produce the highest number of spermatozoan motility in comparison to the other concentrations (5%, 15%, 20% 30%). Sperm preserved with the cryodiluent Alsever's solution along with either methanol or ethanol was found to be effective to fertilize eggs and produce hatchlings. The hatching rates ranged between 1.48% and 14.76%, compare to control. The fish produced through use of cryopreserved sperm and normal sperm were found to grow well and no significant (P<0.05) growth difference was observed between them. In case of silver barb, Barbonymus gonionotus, sperm tested against six extenders such as egg-yolk citrate, urea-egg-yolk, kurokura-1, kurokura-2, 0.9% NaCl and modified fish ringer (MFR) solution. Cryoprotectants used were the same as those of C. carpio. Milt was diluted with the cryodiluent at a ratio of 1:4 for egg-yolk citrate and urea-egg-yolk, 1:5 for kurokura-1 and 1:9 for 0.9% NaCl, MFR and kurokura-2. The cryoprotectant concentration was maintained at 10% of the extender (v/v) in all the cases. Among the extenders, egg-yolk citrate and urea-egg-yolk mixed with 10% DMSO, methanol and ethanol produced 50% post-thaw spermatozoan motility, whereas DMA and glycerol provided only 10% motility. Trials on milt dilution ratio and cryoprotectant concentration are being conducted. Fertilization trials are also underway.

Investment in fish seed multiplication farms in Bangladesh: Evidences of an attractive business

This study mainly evaluated the profitability of Fish Seed Multiplication Farms (FSMFs) having hatchery, nursery and hatchery-cum-nursery located in the districts of Jessore, Jhenidah and Narail in Bangladesh. The general findings of the study were that the investment in FSMFs with hatchery, nursery and hatchery-cum-nursery was highly profitable business. The results clearly indicated that the investment on hatchery was the most profitable than those of nursery and hatchery-cum-nursery operations from the viewpoints of individual investors. The results of sensitivity analysis suggested that the investment in nursery farm was a risky business with 20 per cent increase in operation and management as well was production costs or 20 per cent reduction in benefits if other things remaining the same. It was also evident from the study that the investors of FSMFs had currently been facing some crucial problems, which among others are: problems of inbreeding, shortage of brood fish, incidence of diseases, unavailability of certain inputs and lack of credit.

Seed production and culture techniques of Genetically Improved Farmed Tilapia (GIFT) in brackishwater environment

Effects of different levels of salinity on survival, growth and gonadal development of Genetically Improved Farmed Tilapia (GIFT) were studied under laboratory conditions in glass aquarium, for a period of ten weeks. The initial individual size of the GIFT was 20.23±4.45 and the salinity levels tested were 0, 5, 10, 15 and 20 ppt. The highest survival of 87.5% was found in 0 ppt and the lowest 60.5% in 20 ppt. Though the survival decreased progressively with increased salinity, there were no significant differences (P>0.05) among 0, 5, and 10 ppt. Similar to what has been observed in survival, the specific growth rate (SGR %/day) also decreased as of 1.30, 1.24, 1.08, 0.90 and 0.71, respectively, with the increased salinity of 0, 5, 10, 15 and 20 ppt. The gonadal development was highest in 0 ppt with a GSI value of 3.75 and lowest of 2.01 in 20 ppt. In the second experiment, gonadal development and seed production performance of GIFT in brackishwater condition were investigated for a period of three months. Each of the three fine meshed hapas of 20 square meters made from nylon net was placed in a freshwater (0 ppt) and in a brackish water (10-15 ppt) pond of the Brackishwater Station (BS). GIFT of 65 g average weight from a single cohort were stocked into three hapas at a rate of 2 per m. The male vs female ratio was 1:3. The development of gonad was faster with the higher gonadosomatic index (GSI %) of 3.85 % in freshwater condition than that of 2.73 % in brackish water. Within three months of the study period, a total of 70,510 and 44,250 GIFT fry were produced respectively, in freshwater and brackishwater conditions. Finally under third experiment, a participatory on-farm trial was carried out to evaluate the production performance of GIFT in monoculture and in polyculture with silver barb in coastal freshwater pond conditions. Nine ponds were selected for three treatment combinations of GIFT monoculture (T1), GIFT and silver barb polyculture (T2), and silver barb monoculture (T3). The ponds have been stocked in April, 05 at a density of 25,000 fry per ha. Fishes were fed with rice bran at the rate of 6% bw per day. In one month culture period, GIFT attained an average weight of 16.27 g in monoculture and 17.23 g in polyculture, against an average stocking weight of 0.37 g. Silver barb reached an average weight of 16.62 g in polyculture with GIFT and 10.01 g in monoculture, against an average stocking weight of 3.79 g.

PICES Press, Vol. 6, No. 2, July 1998

CREAMS, PICES and the exploration of the Japan/East Sea The state of the eastern North Pacific from September 97 to February 98 The state of the western North Pacific in the second half of 1997 The status of the Bering Sea in the second half of 1997 Hyung Tack Huh Report on GOOS Living Marine Resource Panel Meeting Global connections: A report of the GLOBEC International Open Science Meeting Update on U.S. GLOBEC research projects and coordination activities in the Northeast Pacific Institutional framework for oceanographic research in Japan The Kuroshio Edge Exchange Processes (KEEP) Project Report on NPAFC Workshop on Climate Change and Salmon Production A new ocean time series station in the western subarctic Pacific

PICES Press, Vol. 6, No. 1, January 1998

Highlights of PICES VI The state of the eastern North Pacific in the first half of 1997 The state of the western North Pacific in the first half of 1997 The status of the Bering Sea in the first eight month of 1997 Organization of fisheries, environmental and ocean science in Canada Richard James Beamish PICES-GLOBEC Climate Change and Carrying Capacity Program Epipelagic fish production in the open Subarctic Pacific: bottom up or self-regulating control? Activity Report of SCOR Working Group 105 Establishment of Marine Information Research Center: new strategy on oceanographic data management in Japan Bering Sea Ecosystem Biophysical Metadatabase: a collaborative research tool for fisheries-oceanography and ecosystem investigations

Carmel River Lagoon Enhancement Project: Water Quality and Aquatic Wildlife Monitoring, 2005-6

In summer and fall 2004, the California Department of Parks and Recreation (DPR) initiated the Carmel River Lagoon Enhancement Project. The project involved excavation of a dry remnant Arm of the lagoon and adjacent disused farmland to form a significant new lagoon volume. The intention was to provide habitat, in particular, for two Federally threatened species: the California Red-Legged Frog, and the Steelhead Trout (South Central-Coastal California Evolutionary Significant Unit). DPR contracted with the Foundation of California State University Monterey Bay (Central Coast Watershed Studies Team, Watershed Institute) to monitor water quality and aquatic invertebrates in association with the enhancement, and to attempt to monitor steelhead using novel video techniques. The monitoring objective was to assess whether the enhancement was successful in providing habitat with good water quality, adequate invertebrate food for steelhead, and ultimately the presence of steelhead. (Document contains 102 pages)

Tidal oscillations at the head of Monterey Submarine Canyon and their relation to oceanographic sampling and the circulation of water in Monterey Bay. Annual report, Part 6, September 1972

During a 25-hour hydrographic times series at two stations near the head of Monterey Submarine Canyon, an internal tide was observed with an amplitude of 80 to 115 m in water depths of 120 and 220 m respectively. These large oscillations produced daily variations in hydrographic and chemical parameters that were of the same magnitude as seasonal variations in Monterey Bay. Computed velocities associated with the internal tide were on the order of 10 em/sec, and this tidally induced circulation may have a significant role in the exchange of deep water between Monterey Submarine Canyon and the open ocean. (PDF contains 49 pages)

Results of the Sea Grant fishes sampling program for the 1971-1972 Season. Annual Report, Part 6, 1973

(PDF contains 48 pages)

Coastal Pelagic Fishes (Report of Working Group 3); Subarctic Gyre (Report of Working Group 6)

This is a report of PICES Working Group 3 (Coastal Pelagic Fishes) for 1993 and the first Annual Report of the Subarctic Gyre Working Group (WG-6). (PDF contains 131 pages)

Ras Behari’s guide to buying fish seed

Document contains 4 pages.

Seed Longevity of Melaleuca quinquenervia: A Burial Experiment in South Florida

Burial and removal techniques with seed bags were used to examine the viability and longevity of Melaleuca quinquenervia seeds at four field sites representing different soil types and hydrological conditions in South Florida. Seed viability was determined over different burial durations in the soil through a combination of germination tests and 2,3,5-triphenyl- tetrazolium chloride (TTC) treatments. Control seeds kept dry at 25 C in the laboratory maintained same viability of ca. 15% over the 3-year study. In the field, seed viability decreased with increased burial duration.(PDF has 4 pages.)

STREAM Journal, Vol. 6, No. 1, pp 1-21. Jan-March 2007

CONTENTS: Approaches to understanding pond-dike systems in Asia: the POND-LIVE project approach, by Dave Little, Marc Verdegem and Roel Bosma. The contribution of fish ponds to nutrient cycling in integrated farming systems, by P.N. Muendo, J.J. Stoorvogel and Marc Verdegem. Improving the contribution of fishfarming to livelihoods in Northeast Thailand, by Chittra Arjinkit, Roel Bosma, Danai Turongrouang. Benefits of pond-dike systems in Bangladesh, by M.S. Kabir, M.A. Wahab and Marc Verdegem. Common carp increases rohu production in farmers ponds, by Mohammad Mustafizur Rahman, Md. Abdul Wahab and Marc C.J. Verdegem. Improving pond-dike farming systems in the Mekong delta, Vietnam; the Can Tho approach, by Dang Kieu Nhan, Le Thanh Duong, Le Thanh Phong, Roel H. Bosma and Marc C.J. Verdegem. Fuzzy pathways for farm development in Vietnam, by Roel H. Bosma, Le Thanh Phong, and Dang Kieu Nhan.

Litter dynamics and phenology of Melaleuca quinquenervia in south Florida

We monitored litterfall biomass at six different sites of melaleuca (Melaleuca quinquenervia (Cav.) S.T. Blake) forested wetlands in South Florida from July 1997 to June 1999. Annual litterfall of melaleuca varied between sites from 6.5 to 9.9 t dry wt ha(-1) yr(1) over the two-year period. Litterfall was significantly higher (p < 0.0001) in scasonally flooded habitats (9.3 t ha(-1) yr(1)) than in non-flooded (7.5 t ha(-1) yr(1)) and permanently flooded habitats (8.0 t ha(-1) yr(1)). Leaf fall was the major component forming 70% of the total litter, woody material 16%, and reproductive material 11%. Phenology of flowering and leaf flush was investigated by examination of the timing and duration of the fall of different plant parts in the litter traps, coupled with monthly field observations during the two-year study. In both years, flowering began in October and November, with peak flowers production around December, and was essentially completed by February and March. New shoot growth began in mid winter after peak flowering, and extended into the spring. Very little new growth was observed in melaleuca forests during the summer months, from May to August, in South Florida. In contrast, the fall of leaves and small wood was recorded in every month of the year, but generally increased during the dry season with higher levels observed from February to April. Also, no seasonality was recorded in the fall of seed capsules, which apparently resulted from the continual self-thinning of small branches and twigs inside the forest stand. In planning management for perennial weeds, it is important to determine the period during its annual growth cycle when the plant is most susceptible to control measures. These phenological data suggest that the appropriate time for melaleuca control in South Florida might be during late winter and early spring, when the plant is most active.

Cruise Report 69-S-6: Bottomfish Program

(PDF contains 2 pages)