Studies on the egg quality of Penaeus monodon Fabricius based on morphology and hatching rates

Eggs of P. monodon are classified into 5 different types on the basis of morphological criteria and hatching rates: A sub(1) eggs, which undergo normal development with 58% hatching rate; A sub(2) eggs, which show delayed and/or abnormal development with 32% hatching rate; B eggs, which are unfertilized and characterized by irregular cytoplasmic formation; C eggs, which are unfertilized and show no change in appearance; and D eggs, which are unfertilized and show extensive bacterial presence. The establishment of a highly linear relationship between percent A sub(1) eggs and hatching rate provides a useful tool to aid the hatchery technician in determining which spawnings to reject outright and which to rear through the larval and post-larval stages.

Effect of substrate types on fecundity and nauplii production of ablated Penaeus monodon Fabricius

Experiments were undertaken to assess the survival, spawning, fecundity and nauplii production of ablated P. monodon females reared in flow-through broodstock tanks with white coralline and black sand substrate for 62 days. The similar trend observed in mortality rates in both substrates suggests that variation in substrate material for broodstock tanks is not a likely cause of prawn mortality. There were also no significant differences observed between rematurtion rates, i.e. number of spawnings, under the different treatments. Singnificantly higher nauplii production were observed in females in tanks with white substrates. At present, the land-based broodstock tanks in SEAFDEC utilize white coralline substrates due to higher hatching rate of eggs and nauplii production, convenience in siphoning out debris and excess food that tend to accumulte in the tank, and contrast provided by the white substrate during nightly observations of ovaries.

Evaluation of milkfish (Chanos chanos Forskal) and prawn (Penaeus monodon Fabricius) in polyculture systems

The growth, survival and profitability of C. chanos and P. monodon grown in 5 different combinations for 100 days in 500m2 brackish water ponds were assessed. Differences in the growth and production of prawns cultured singly or in combination with milkfish at increasing stocking density strongly suggests that the presence of milkfish exerts some negative effect on prawn. However, growth production and competition index data suggest that the presence of prawn do not significantly affect milkfish. While the maximum production of prawn can be attained in monoculture, its polyculture with 2000 milkfish/ha is also economically feasible.

Growth and survival of Penaeus monodon Fabricius zoeae on different diatom feeds

A study was undertaken to determine the most suitable food for Penaeus monodon zoea out of mixed diatoms, Skeletonema costatum, Chaetoceros calcitrans and C. gracilis. Results indicate that mixed diatoms and C. gracilis are the better food. Addition of zooplankton, e.g.rotifers, is also recommended as early as Z3 since the larvae start to be omnivorous at this stage.

Polyculture of milkfish (Chanos chanos) and tiger prawn (Penaeus monodon Fab.) with and without supplemental feeding

Twelve 1,000 m super(2) earthen ponds were used to compare the growth rates, survival and production of milkfish (Chanos chanos)) and prawn (Penaeus monodon ) in monoculture and polyculture systems in shallow brackishwater ponds and without supplemental feeding. The low production and survival rates obtained were attributed to the lack of natural food; the high salinity during the first month of culture could be one of the causes of the high mortality of prawn observed in both mono and polyculture systems. Although the results of the trial were not ecouraging, it is possible that high yields may be obtained from the combination of the 2 species given enough natural food and favourable water conditions.

Effects of water movement and aeration system on the survival and growth of hatchery bred sugpo (Penaeus monodon Fabricius) in earthen nursery ponds

An experiment was conducted to determine the effects of water movement and airlift aeration on the survival and growth of P. monodon fry reared from P sub(4)P sub(5) to P sub(32)P sub(33) in earthen brackishwater ponds. The high survival rates obtained justify the need for aeration when using the earliest stages of fry (P sub(4)P sub(5)) at higher stocking densities. For older stages regardless of source and at lower stocking densities, nursery operations based on traditional methods could also achieve better survival rates.

In-vitro effect of fungicides on hyphal growth and sporogenesis of Lagenidium sp. isolated from Penaeus monodon larvae and Scylla serrata eggs

The sensitivity of Lagenidium, isolated from Penaeus monodon, Scylla serrata , to 34 antimycotics was determined. Effects on the development of vesicles, zoospores and mycelial growth were evaluated. Although mycoidal levels of the chemicals tested will be ideal for lethal treatment on control of the fungus, the high dose required may be lethal to the host, thus the use of mycostatic concentrations is more practical. Treatments of rearing water containing larvae, adult shrimps or crabs should be done only after preliminary tolerance experiments using at least the mycostatic dose prove to be safe for the hosts. Mycocidal doses can be used for determining disinfection doses of equipment and facilities used in rearing procedures as well as for destroying batches of infected larvae.

Feeding behavior and food preference of Penaeus monodon Fabricius with scrap tilapia

The time of day during which P. monodon feeds at different depth levels in earthen ponds, and its preference for three types of tilapia feeds (dry, fresh and fermented) were determined. It was observed that P. monodon concentrated at the bottom beds during the day and along the periphery of dikes during night-time, with a slight tendency to swim and feed towards the surface as darkness increased. P. monodon showed special preference for dried tilapia compared to fresh and fermented tilapia. P. monodon also showed adaptability to the platform method of feeding, especially during night-time.

Survival, growth and production of Penaeus monodon at different stocking densities in earthen ponds with flow-through system and supplementary feeding

The survival, growth and production of Penaeus monodon in earthen ponds were observed. A significant effect of stocking density on the survival rate of prawns was observed. The final average weight obtained varied inversly with the stocking density and the production varied directly with stocking density and survival rates. The maintenance of a clear water environment is recommended as a preventive measure against Vorticella attack.

Improvement of diet attractability for Penaeus monodon by supplementing various attractants

An experiment was undertaken to test the effect of supplemental krill meal, earthworm meal, glycine, sucrose or mussel water on diet attractibility of Penaeus monodon. Only glycine or mussel water significantly improved diet attractibility

Survival, growth and production of Penaeus monodon and P. indicus at different density combinations with milkfish

Penaeus monodon and P. indicus juveniles were stocked and reared for about 3 months in earthen ponds at different density combinations with Chanos chanos. The presence of either Penaeus species at any density ratio did not affect significantly the C. chanos survival. Survival rates of the penaeids indicated that intraspecific and interspecific competition occurred and were reduced with the reduction in stocking rate. It is concluded that further studies on higher density ratios and feeding and economic consideration would be of help to the development of this kind of fishpond management system.

Survival and changes in the fine structure of selected tissues of Penaeus monodon Fabricius juveniles fed various carbohydrates

Penaeus monodon juveniles were reared on semipurified diets containing various carbohydrates (maltose, sucrose, dextrin, molasses, cassava starch, corn starch or sago palm starch). Significant differences were observed between the type as well as the level of carbohydrate in the diet on the survival of the juveniles. Results indicate that there does not seem to be any correlation between survival and the complexity of the carbohydrates.

The effect of various protein levels on the growth and survival rates of Penaeus monodon Fabricius

Postlarvae of tiger prawns, P. monodon, were fed with various protein levels of 0; 30; 40 and 50%, fish meal and rice bran were combined and pelletized for 16 wks. Prawns used had an average mean length of 1.2 mm and an average mean weight of 5.5 mg. Nitrite, ammonia, dissolved oxygen content, temperature and pH were monitored. After one month of feeding, growth increments of postlarvae fed with 30% protein diet were significantly different from those given 0; 40 and 50%. Survival rate of the group fed 40% protein was higher (81.7%) but was not significantly different from those fed 30% (68.0%). The highest feed efficiency was obtained from the 30% protein pellets (4.6). At the end of 16 weeks, growth increment of 40% protein level was significantly different from the 30% and 50% protein levels, but the survival rate was not significantly different from the 30% protein level. Feed conversion for the 40% protein diet was 2.8.

Bacteria from seawater used in Penaeus monodon larval cultures

Bacteria in the seawater used in P. monodon hatchery operations were isolated on Bachmann's agar. The total plate counts in 25 isolations ranged from 1.0 - 5.0 x 10² to 5.1 -10.0 x 10⁵ cells per ml. Out of 124 isolates, 98 (79 percent) were Gram-positive and 26 (21 percent) were Gram-negative. Micrococcus and Staphylococcus were dominant in the former group, while Acinetobacter, Moraxella, Flavobacterium and Alcaligenes were most numerous in the latter. Twenty-nine of the Gram-positive isolates closely resembled Peptostreptococcus, Planococcus, and Pediococcus.

Effect of temperature on the oxygen consumption of Penaeus monodon postlarvae

The study aimed to determine the oxygen consumption of P. monodon postlarvae at different temperatures. Results suggest dependence of oxygen consumption on both weight of postlarvae and temperature. The relationships appear linear at the temperature range examined. Temperature dependence of oxygen consumption suggests that oxygen requirement (and metabolism) increases with temperature.