Polyculture of gulsha (Mystus cavasius Ham.) with rajpunti (Puntius gonionotus Bleeker) and silver carp (Hypophthalmichthys molitrix Val.) in earthen ponds

Culture of gulsha (Mystus cavasius) with rajpunti (Puntius gonionotus) and silver carp ( Hypophthalmichthys molitrix) was undertaken to assess the growth and production potential of these species under polyculture system. Fingerlings of gulsha, rajpunti and silver carp were stocked at a density of 18.000, 10.000 and 4.000/ha respectively. Two treatments were tested in this experiment. Treatment-I was conducted with rice bran and mustard oil cake and treatment-II with rice bran and duck weed. All the ponds were fertilized with urea and TSP at fortnightly intervals. After six months' rearing, the gross production was estimated to be 3,582 and 3,125 kg/ha from treatment-I and treatment-II respectively. Total yield showed non-significant differences (P>0.05) between the treatments.

Population dynamics of Harpodon nehereus (Ham.-Buch.) from the Kutubdia channel of Bangladesh

ELEFAN-Φ, ELEFAN-I, ELEFAN-II were used to estimate the parameters of population of Harpodon nehereus from length-frequency data collected from Kutubdia channel of Bangladesh coastal water. The Lα and K were found to be 24.48 cm and 1.50/year. The annual rate of natural mortality (M) and fishing mortality (F) were found to be 2.46 and 3.27 respectively. The rate of exploitation (E) was estimated as 0.57. The mean length at first capture (Lc) was estimated as 6.747 cm. This species was recruited in the fishery during March to May, August and October. The Peak recruitment appeared between March to April. Emax. was found to be 0.501. The present investigation clearly showed the over fishing (E>0.50) of H. nehereus in the investigated area of Bangladesh coastal water.

Effect of mola (Amblypharyngodon mola Ham.) on the growth and production of carps in polyculture

The effect of introduction of mola (Amblypharyngodon mala) in polyculture with rohu (Labeo rohita), catla (Catla catla) and mirror carp (Cyprinus carpio var. specularis) was studied in semi-intensive culture system in the pond complex of the Fisheries Faculty, Bangladesh Agricultural University, Mymensingh. Mala, a small indigenous fish was found to exert a negative impact on growth and production of carps. After four months' rearing, significant difference (P<0.05) was observed in the net production between the treatments.

Estimation of maximum sustainable yield (MSY) of hilsa (Tenualosa ilisha Ham.) in the Meghna river of Bangladesh

MSY per recruit of Tenualosa ilisha in the Meghna river was predicted as 112 g per recruit at the F(msy)=0.6/yr and at T(c)=0.6/yr. But Y/R=95 g per recruit was obtained at the existing fishing level, F=1.14/yr and at T(c)=0.6/yr. Existing F level was nearly double than the F(msy) level. Fishing pressure should be reduced immediately from F=1.14/yr to F(msy)=0.6/yr. F(msy)=1.14/yr was the same at first capture, T(c)=1.0, 1.2 and 1.4/yr, and MSY could be obtained as 142 g, 162 g and 176 g per recruit respectively. It is easier to change the first capture age (Tc) rather than changing off level. So, hilsa fishery manager may adopt F(msy)=1.14/yr while age at first capture must be increased from T(c)=0.6/yr (3 cm size group) to T(c)=1.4/yr (25 cm size group), by which 1.8 times production could be increased than the present production. MSY also possible to obtain as 201 g and 210 g per recruit at F(msy)=2.0/yr and 4.0/yr at T(c)=1.7/yr and 1.9/yr respectively. Under both the situations, hilsa production could be increased 2 times than the present production. To obtain the MSY=210 g per recruit the fishing level could be increased up to F=4.0/yr at T(c)=1.9/yr (34 cm size group). Economic point of view, hilsa fishery managers may choose to obtain the economic MSY as 201 g per recruit at F(msy)=2.0/yr and T(c)=1.7yr (31 cm size group) in the Meghna river of Bangladesh.

Studies on the gonadosomatic index and fecundity of chapila (Gudusia chapra Ham.)

The study was conducted in pond to determine the fecundity and gonadosomatic index of chapila, Gudusia chapra. The male fish was found to attain sexual maturity at 7.7 cm and 7.41 g and that of the female at 9.3 cm and 14.65 g by standard-length and body-weight respectively. The investigated fishes were found to be male and female at the ratio of 1:3 and generally female was found to be larger than male. The fish was found to spawn for several months with two spawning peaks, one in April and another in August as indicated by the peaks of gonadosomatic index and ova diameter. Fecundity of the fish ranged from 25,220 to 154,528 with an average value of 72,383 and was found to increase with the increase in length and weight of the fish. The relationships between fecundity and standard-length, body-weight, gonad-length and gonad weight of the fish were found to be linear and significant.

Effects of periphyton grown on bamboo substrates on growth and production of Indian major carp, rohu (Label rohita Ham.)

The effects of periphyton, grown on bamboo substrates, on growth and production of Indian major carp, rohu, Labeo rohita (Hamilton), were studied in 10 ponds during July to October '95 at the Bangladesh Agricultural University, Mymensingh. Five ponds were provided with bamboo substrates (treatment I) and the rests without bamboo substrates (treatment II). It was revealed that there had been no discernible difference in the water quality parameters between treatments. A large number of plankton (30 genera) showed periphytic nature and colonized on the bamboo substrates. The growth and production of fish was significantly (p<0.05) higher in the ponds with bamboo substrates as compared to the ponds without substrates. The net production of rohu in treatment I was about 1.7 times higher than that of treatment II. Fish production was as much as 1899 kg/ha over a culture period of 4 months in the periphyton-based production system.

Study on the intestinal bacteria of Labeo rohita (Ham.)

The quantitative and qualitative aspects of intestinal bacteria of rohu fish (Labeo rohita) showed that total viable count of bacteria ranged from 9.9 x 106 to 1.4 x 107 CFU/g of intestine in different age groups of fish. The bacterial load was highest in the month of July and lowest in January. The genera of the isolates from intestine included Coryneform, Micrococcus, Flavobacterium, Cytophaga, Achromobacter, Aeromonas Enterobacteriaceae and Vibrio. Coryneform was the dominant group throughout the study period followed by Micrococcus and Enterobacteriaceae. Marked variations in the bacterial load and generic composition of intestinal bacteria were evident during the study period in different age groups of rohu fish.

Toxic effects of dimecron to fingerlings of Labeo rohita (Ham.) during acute and chronic exposures

The toxic effects of dimecron on growth, body composition and oxygen consumption of fingerlings of Labeo rohita were studied. Dimecron concentrations of 4 and 8 mg/l were used. Both acute (3-h) and chronic (15- 42 d) exposure schedules were followed. Compared with the control fish, both 4 and 8 mg/l dimecron treatment significantly suppressed weight gained in fish by 9.71% and 30% respectively during a 42 day exposure period. However, the length of fish was suppressed by 11.46% significantly only in fish group exposed to 8 mg/l dimecron. Similarly, the protein content was also significantly reduced in the above group of fish. The oxygen consumption of fish was elevated considerably, but not significantly in both group of treated fish (8.5% and 26.07%) during acute exposure. However, after 15 days of exposure the rate decreased by 18.98% significantly only in fish exposed to 8 mg/l dimecron. The threshold level of DO at low oxygen environment found to be slightly higher in fish at 8 mg/l dimecron. The survival time at the above oxygen condition was reduced during acute exposure (3-h) and that was extended during chronic (15-d) exposure.

Energetics of resting metabolism in an Indian major carp (Catla catla Ham.)

Resting metabolism in Indian major carp, Catla catla Ham. fingerlings were investigated. For this purpose a water recirculatory system in the laboratory was used. The metabolic energy losses were determined by the indirect method of oxygen consumption by the fish and were then multiplied by an oxycalorific coefficient (Q-ox). Five metabolism chambers in the experimental system were used where there were two same treatment runs in quadruplicate of mean total weight of fish fingerlings of 109.5, 110.4, 112.8 and 111.6g/chamber. The water temperature in the system was 28±0.5°C. The mean metabolic rate in the replicates showed no significant variation (p>0.05) and was found to be 151.66, 153.91, 150.25, 152.74 mgO-2/kg/h respectively. This showed an equivalent energy loss 5.40, 5.52, 5.51 and 5.56 KJ/chamber/day (35.60, 35.92, 36.67 and 36.40 KJ/kg/day) respectively. Energetics of resting metabolism in an Indian major carp (Catla catla Ham.)

Pathogenicity of Aeromonas hydrophila artificially infected to Indian major carp (Cirrhinus mrigala Ham.)

Abrasion, feeding, injection and immersion methods were used to evaluate the pathogenicity of five different strains of Aeromonas hydrophila viz. RG (rui gill), ML (mrigal lesion), SG (sharpunti gill), F1K (mrigal kidney), GFL (gold fish lesion) and Ah-19 (Aeromonas hydrophila-19, Ref. Strain) against C. mrigala H. Bacterial suspension containing viable cells of 7.5x 10⁵ per ml was found to be very effective in intramuscular injection and feeding resulting 100% mortality after 96hr of inoculation. The strain RG, ML and F1K produced scale loss with erosion of the skin surface with/without hemorrhagic lesion after 48hr of inoculation following abrasion method. The strains SG and Ah-19 resulting scale loss with erosion of the skin surface with/without hemorrhagic lesion after 72hr of inoculation following abrasion and injection methods. SG and F1K caused reddening in mouth region after 72hr of feeding inoculation, whereas RG resulted frank ulcers from eroded dermal layer exposing underlying musculature which was hemorrhagic after 96hr of inoculation by abrasion method.

Histological distribution and ultrastructure of exocrine pancreas in Indian major carp (Labeo rohita Ham.) and its alteration in aflatoxicosis

The distribution pattern of exocrine pancreas in Labeo rohita besides its general location along the course of intestinal mesentery was studied. It is evenly distributed within the liver around portal vessels and also within the spleen near a blood vessel. On ultrastructure, two cell types of different degrees of staining intensities containing abundant rough endoplasmic reticulum, mitochondria, pre-zymogen and zymogen granules were marked. During aflatoxicosis, the mesenteric pancreas and hepatic pancreas were mostly affected revealing necrotic changes to acini. The zymogen granular activities were markedly reduced. Ultra structurally, the rough endoplasmic reticulum was fully dilated and formed whorled pattern. The damage to the exocrine pancreas might be affecting digestive enzymes' secretion which may be one of the causes of aflatoxin-induced anorexia in fish.

Some aspects of population dynamics of juvenile hilsa shad (Tenualosa ilisha Ham.) from the Meghna river, Bangladesh

Population dynamics of the juvenile hilsa shad (Tenualosa ilisha) in the nursery ground of the Meghna River have been studied on the basis of the length cohort analysis of 8023 specimens. The growth parameters viz; asymptotic length (Lα), curvature character (K) and initial time (t0) were found to be 30.69 cm, 1.2 yrˉ¹ and 0.45 yrˉ¹ respectively. Curvature parameter indicates that jatka is a fast growth performer. The natural, fishing and total mortality were found to be 1.37 yrˉ¹, 1.41 yrˉ¹ and 2.78 yrˉ¹ respectively. Survival rate (S) was found to be 6.2%. A small difference was found between the age at first capture (Tc) and the recruitment age (Tr). Stocks of jatka seem to be overexploited and need to be conserved.

Effects of animal and plant protein diets on growth of Indian major carp (Labeo rohita Ham.) fingerlings

An experiment was conducted with Labeo rohita fingerlings in an indoor static fish rearing water system of glass made aquaria. Five experimental diets A, B, C, D and E were formulated containing 33% dietary protein level in five treatments each having two replicates containing 12 fingerlings of mean total initial weight of 13.00±0.2g. Sixty days of feeding trial in this experiment showed that fish fed on diet 'A' containing fish meal and diet 'E' containing mixed plant sources protein had significantly highest and lowest growth respectively. However, no significant difference of growth was found in fish fed on diets C and D containing meat and bone meal, and mix of animal protein source diets respectively. The result showed that the apparent protein digestibility (APD) of diets 'A' and 'E' had significantly best and least values respectively. Food conversion ratio (FCR) and protein efficiency ratio (PER) ranged between 1.37 to 2.17 and 1.38 to 2.18 respectively. On the basis of observed FCR and PER diets 'A' and 'E' produced significantly highest and lowest growth respectively.

Domestication of an endangered fish species Nandus nandus (Ham.). Pt. 1. Laboratory rearing of young fish up to sexual maturity

An experiment was conducted for rearing of Meni, Nandus nandus in laboratory condition for seven months with the objective to select appropriate feed for the species and to develop a rearing technique of the species up to the stage of sexual maturation. Different trials were conducted using artificial feed (35.5% protein), dead fresh kachki (Carica soborna), dead fresh prawn (Macrobrachium lamarrei) and live prawn (Macrobrachium lamarrei). The provision of bottom sediment did not significantly influence the growth of fish. Between dead fresh kachki and dead fresh prawn, the fish preferred dead fresh prawn. The fish was found to be reluctant to take dead fresh kachki and prawn as food unless they became very hungry. The fish was found actively feeding on live prawn. The FCR of the prawn as food for N. nandus was found to be 2.5. From the study, it was observed that in laboratory rearing N. nandus preferred live prawn as food than artificial feed, dead fresh kachki and dead fresh prawn. The fish fed on live prawn became sexually matured (eggs or white milt extruded by gentle pressure on the abdomen of the fish) in the laboratory at the end of the experiment.

Pathology of mixed infections of saprolegniasis-myxosporidiosis in Indian major carp (Catla catla Ham.)

An outbreak of saprolegniasis in Catla catla in composite carp culture ponds were recorded during winter season. The typical cotton wool growths were observed on whole body surfaces of catla along with sporadic mortality. The fungal invasion was only restricted to skin and no fungal elements were visible in any internal organs after periodic acid schiff staining. On histology, periportal accumulation of mononuclear cells in liver, presence of myxosporidean cysts in antieror kidney, eosinophilic granular cells reaction in submucosa of stomach and intestine, dilated and engorged blood vessels of brain along with sloughing of epidermis and hyperplasia at gill lamellar base were pronounced changes. The possible role of release of Saprolegnia toxin in producing internal organs pathology has been discussed.