Establishment of satellite nucleus of genetically improved farmed tilapia (GIFT strain) in Bangladesh

The aim of this study was to evaluate growth performance of the GIFT (Genetically Improved Farmed Tilapia) strain after one generation of selection for increased body weight at Bangladesh Fisheries Research Institute. Founder stock comprised of 30 families having 300 individuals of the GIFT strain were introduced from Malaysia through \X!orldFish Center in March 2005. The founder stock was reared in 100 m2 hapa for three months and then individually tagged using Passive Integrated Transponder (PIT) at the weight between 30 and 40 g. After tagging, all the fish were communally grown out in pond until harvest. Breeding value for body weight was estimated using SAS and ASREML ranging from 4.17 to 9.70 g for males and 4.24 to 9.36 g for females. The best 40 females and 40 males from the founder stock were then selected to produce progeny of the first generation (F-1). From each family 25 female and 25 male fingerlings were sampled and tagged using PIT. A total of 2,000 tagged fish from 40 families were stocked in a pond (1000 m2) for a continuation of the selection program. In addition, surplus fish after tagging were also reared together with progeny of the founder stock in cistern ecology for growth evaluation. The mean weight of the F-1 generation of GIFT fish was 7.2% greater than that of the founder population (non selected population).

Over-wintering performance of mixed sex and monosex tilapia (Oreochromis niloticus L.) in Bangladesh

An experiment was conducted for six months in 6 experimental ponds (each size 80 of m2) to assess the over-wintering performance between mixed sex and monosex tilapia, Oreochromis niloticus. The experiment was carried out with two treatments each with three replicates. In the first treatment (T1), mixed sex tilapia were stocked in 3 ponds with a mean initial of 4.80±0.18 g. In the second treatment (T2), monosex tilapia were stocked in another 3 ponds with a mean initial weight of 4.81 ±0.20 g. Each pond was stocked with 250 fingerlings. Fish were fed at the rate of 6% of fish body weight at the beginning. The feeding rate was gradually reduced to 2% for the third month and finally increased to 3% for rest of the period. Water quality was monitored fortnightly and the ranges were: temperature17.86-29.10°C, dissolved oxygen 4.25-6.10 mg/1, pH 6.97-7.20 and transparency 24.10-36.50 cm. After 6 months of rearing monosex tilapia attained a significantly (ptilapia. Monosex tilapia also resulted in significantly (Ptilapia. However, there was no significant (ptilapia. The production of monosex tilapia (3723.10 kg/ha) was about 32% higher than that of mixed sex tilapia (2776.28 kg/ha). The net profit/ha generated from the 6 months culture period was calculated as Tk. 43,311.45 and. 69,277.32/- for mixed sex and monosex tilapia respectively. The results of the present study suggested that it is possible to successfully culture tilapia during the winter period and the culture of monosex tilapia is more profitable due to its higher growth rate than that of mixesex tilapia.

Effect of salinity on food consumption and growth of juvenile Nile tilapia (Oreochromi niloticus L.)

The effect of salinity (0, lO and 20%o, water temperature 28 ± l oC) on food consumption and growth of juvenile Nile tilapia, Oreochromis niloticus L. (9.94 ± 0.15 g) were investigated by feeding group of 20 fish at 2% body weight day. Individual food consumption was measured using X-radiography. There were no significant differences in growth or white muscle protein concentrations among groups. During feed deprivation, weight loss was similar for fish held at O%o and 10 %o salinity, but after 7 days over 50% of the fish maintained at 20%o salinity developed lesions covering 5-25% of the body. No significant relationships were observed between individual specific growth rates and food consumption rates within the groups. The fish in all salinity groups showed a negative correlation between specific growth rate and food conversion ratio. The coefficient of variation for wet weight specific food consumption and the mean share of meal for each fish were used as a measure of social hierarchy strength. A negative correlation was observed between coefficient of variation in food consumption and mean share of meal. The social hierarchy structure was similar in all salinities; 25% of the fish were dominant (18.29% above an equal share of meal) and 30% were subordinate (16.19% below an equal share of meal) and the remainder 45% fish fed theoretical share of meal (MSM, 5.26%).

Performance of genetically improved farmed tilapia (GIFT) under mono and mixed cui ture with silver barb (Barbodes gonionotus) in south-west Bangladesh

A participatory on-farm trial was carried out to evaluate the production performance of GIFT (genetically improved farmed tilapia) strain of Oreochmis sp., either alone or with silver barb (Barbodes gonionotus), in six rain-fed freshwater ponds of coastal area. There vvere two treatments; (i) GIFT alone at a stocking density of 24,700/ha (T1) and (ii) 1:1 combination of GIFT and silver barb (T1). Each of the treatments had three replications. A significantly (p

Morphometric characteristics and meristic traits of Tilapia zillii from three major dams of a southwestern state, Nigeria

Tilapia zillii was examined in three major dams in Ekiti State, Nigeria (Ureje dam, Ado, Egbe dam and Ero dam) for their morphometric characteristics and meristic traits. The results showed that the average body weight of the fish sampled were 145.86±50.06g in Ado, 30.19±9.35g in Egbe and 40.58±52.30g in Ero dam. Average total lengths were 21.29±3.20cm, 12.32±1.74cm and 11.24±1.56cm for Ado, Egbe and Ero dam respectively. The relationship between average weight and length of T. zillii showed that Y=15.202X-179.33 (R2=0.7181), Y=4.40049±-24.075 (R2=0.6708) and Y=2.6676X10.596 (R2=0.0063) for Ado, Egbe and Ero dam respectively. The results on headlength, total length, body weight and standard length and pre-pelvic distance are significantly different at 95% among the T.zillii of the three dams. There was no significant difference in the meristic traits of the T. zillii of the three dams. The population size of T.zillii in the three dams was just too small compared to other species of fish hence the need to assess the stock of fish in these dams critically for the encouragement of proper fishing activities in these dams.

Seed production and culture techniques of Genetically Improved Farmed Tilapia (GIFT) in brackishwater environment

Effects of different levels of salinity on survival, growth and gonadal development of Genetically Improved Farmed Tilapia (GIFT) were studied under laboratory conditions in glass aquarium, for a period of ten weeks. The initial individual size of the GIFT was 20.23±4.45 and the salinity levels tested were 0, 5, 10, 15 and 20 ppt. The highest survival of 87.5% was found in 0 ppt and the lowest 60.5% in 20 ppt. Though the survival decreased progressively with increased salinity, there were no significant differences (P>0.05) among 0, 5, and 10 ppt. Similar to what has been observed in survival, the specific growth rate (SGR %/day) also decreased as of 1.30, 1.24, 1.08, 0.90 and 0.71, respectively, with the increased salinity of 0, 5, 10, 15 and 20 ppt. The gonadal development was highest in 0 ppt with a GSI value of 3.75 and lowest of 2.01 in 20 ppt. In the second experiment, gonadal development and seed production performance of GIFT in brackishwater condition were investigated for a period of three months. Each of the three fine meshed hapas of 20 square meters made from nylon net was placed in a freshwater (0 ppt) and in a brackish water (10-15 ppt) pond of the Brackishwater Station (BS). GIFT of 65 g average weight from a single cohort were stocked into three hapas at a rate of 2 per m. The male vs female ratio was 1:3. The development of gonad was faster with the higher gonadosomatic index (GSI %) of 3.85 % in freshwater condition than that of 2.73 % in brackish water. Within three months of the study period, a total of 70,510 and 44,250 GIFT fry were produced respectively, in freshwater and brackishwater conditions. Finally under third experiment, a participatory on-farm trial was carried out to evaluate the production performance of GIFT in monoculture and in polyculture with silver barb in coastal freshwater pond conditions. Nine ponds were selected for three treatment combinations of GIFT monoculture (T1), GIFT and silver barb polyculture (T2), and silver barb monoculture (T3). The ponds have been stocked in April, 05 at a density of 25,000 fry per ha. Fishes were fed with rice bran at the rate of 6% bw per day. In one month culture period, GIFT attained an average weight of 16.27 g in monoculture and 17.23 g in polyculture, against an average stocking weight of 0.37 g. Silver barb reached an average weight of 16.62 g in polyculture with GIFT and 10.01 g in monoculture, against an average stocking weight of 3.79 g.

Effects of black Bengal goat manure on growth and production of Genetically Improved Farmed Tilapia (GIFT) starin [sic], Oreochromis niloticus

In a goat-tilapia integrated farming system, the effect of Black Bengal goat manure on the growth and production of Oreochromis niloticus was studied at the Freshwater Substation, Shantahar, Bogra for 4.5 months. The stocking density used in three treatments were, 200 goats and 15,000 GIFT strain/ha (T1); 300 goats and 15,000 GIFT strain/ha (T2); and only 15,000 GIFT strain/ha (T3). The initial individual total length and weight of stocked tilapia were 7.6 cm and 11.34 g, respectively. Twelve ponds each having an area of 40 square meters were used for this trail [sic]. On one side of each pond goat shed was constructed and the space allocated for each goat was 0.75 m x 1.5 m. Newly weaned black Bengal goats of average weight 8.45 kg was used in the trial. Every morning the goats manure was swiped out in ponds through the fixed opening of bamboo made floor of goat shed. Water depth of the ponds was maintained at 0.75 m. The highest fish production was in treatment 1 (1,750 kg/ha) followed by treatment 2 (1,455 kg/ha) and treatment 3 (621 kg/ha). The difference in individual goat weight was not significant (P>0.05) among treatments 1 and 2.

Freezing characteristics of tropical fishes 2. Tilapia (Tilapia mosambica)

Tilapia from fresh water and brackish water-sources behaved differently during iced and frozen storage. The former showed an ice storage shelf life of about 13 days while the latter showed signs of spoilage beyond 10 days. In their respective freezing characteristics, the samples from the two sources exhibited far more significant variations. The fresh water type iced for 13 days preserved well for over 24 weeks when frozen and kept at a temperature of -18° C, while the brackish water variety held in ice for 10 days and subsequently frozen gave a shelf life of only 8 weeks under similar conditions.

Mesh selectivity for spotted seer, Scomberomorus guttatus (Bloch & Schneider)

An attempt has been made to evolve a suitable mesh size for the commercially significant size group of S. guttatus. To obtain maximum sustainable yield taking biological factors also into consideration, 52 mm mesh bar nets are suggested for exploiting the fishery on both the East coasts of India.

Spoilage of spotted seer (Scomberomorous guttatus) during ice storage

Quality deterioration of seer held directly in contact with ice, in different forms, fillets and chunks, and of chunks held in ice but without direct contact, was studied for a period of 15 days. While the chunks held out of contact with ice were acceptable up to 13 days based on organoleptic evaluations, the chunks and fillets held in direct contact with ice were acceptable only up to 10 days. The order of preference of the samples at any interval of ice storage was chunks held out of contact with ice>chunks held directly in ice>fillets held directly in ice. The changes in the chemical quality of these samples were also in the same order, the deterioration being maximum in fillets and least in chunks kept out of contact with ice.

Freezing characteristics of tropical fishes III. Spotted seer (Scomberomorus guttatus)

Skin-on fillets of spotted seer were frozen individually with different pre-freezing ice storage periods, and stored at -23°C and -l0°C. The frozen storage shelf life was evaluated, with respect to holding time in ice prior to freezing, by examining the extent of oxidative rancidity, protein denaturation, organoleptic changes etc. Fillets with pre-freezing ice storage periods of 0, 3, 5 and 7 days had frozen storage shelf-life of 32, 24, 20 and 16 weeks respectively at -23°C. The fillets stored in ice for more than 7 days are unsuitable for further processing. Storage temperature greatly affected keeping quality of frozen fillets. Freshly frozen fillets stored at -10°C became unpalatable at 16-20 weeks as compared to 28-32 weeks for the fillets stored at -23°C.

Purification and characterisation of phosphorylase from muscle of Tilapia (Tilapia mosambica)

Phosphorylase from muscle of tilapia (Tilapia mosambica) was extracted by water and purified by ammonium sulphate precipitation, centrifugation and repeated recrystallisation. Electro-phorogram of the enzyme preparation showed a single band near origin. The enzyme showed optimum pH and temperature at 6.1 and 37°C respectively. Glucose and glucose-6-phosphate were found to be competitive inhibitors of the enzyme. Maltose and starch acted as primers for the phosphorylase reaction like glycogen.

Influence of sex, spawning, starvation and water temperature on fatty acid composition in Tilapia mossambica

The influence of sex, spawning, starvation and water temperature on the fatty acid composition of Tilapia mossambica has been studied. Tilapia egg lipid was found to have unusually high percentage of C sub(22:6) fatty acids (9.09%) compared to body and intestinal lipids. The C sub(16:1) acid was much less in the egg lipids (3.5%) whereas it was 11% in the body lipids. There was no significant difference in the fatty acid composition of body and intestinal lipids of male and female tilapia. Starvation caused the presence of high content of lower fatty acids (C sub(6), C sub(8), C sub(30), C sub(12) and C sub(33)) in the body lipids. Water temperature also influenced the fatty acid composition of Tilapia; the difference was more significant in body lipids than in intestinal lipids.

Quality deterioration in tilapia during storage in refrigerated brine

During storage of Tilapia nilotica in refrigerated brine at 4°C, the whole (ungutted) fish had higher levels of free fatty acid and spoiled faster than the gutted fish. The shelf life of whole fish was 16 days and that of gutted 28 days; these values are, at least, as good as those reported for ice storage. Flavour of the cooked fish appeared to be the quahty-hm1tmg factor reducing the shelf-life of gutted tilapia to 28 days even though the raw gutted fish was judged acceptable, after 31 days retaining 65-70% freshness.

Length-weight relationship of Tilapia mossambicus of Idukki Reservoir

The results of ten months study (September 1983 to July 1984) of the length-weight relationship of males and females of Tilapia mossambicus of the reservoir waters of Idukki Hydel Project show deviation from the cubic relationship of these two parameters. This is due to the retarded growth pattern of this exotic species in this oligotrophic habitat.