Diet of Bottlenose Dolphins Tursiops truncatus in the Northwest Panhandle and Foraging Behavior Near Savannah, Georgia

The foraging ecology of bottlenose dolphins Tursiops truncatus in the Northwest Florida Panhandle and estuaries in northern Georgia was determined using diet analysis and behavioral surveys. Stomach content analysis was completed on bottlenose dolphins(N = 25) that stranded in the Northwest Florida Panhandle from November 2006 to March 2009. The most abundant prey species were spot Leiostomus xanthurus (20.4%), squid (10.9%), pinfish Lagodon rhombiodes (10.3%), and Atlantic croaker Micropogonias undulatus (8.5%). Dolphins that stranded during months with a red tide Karenia brevis bloom consumed more pinfish, and spot; whereas dolphins that stranded in non-bloom months consumed more squid, Atlantic croaker, and silver perch Bairdiella chrysoura. Differences in diet were also identified for dolphins that stranded inside bays/sound and dolphin that stranded outside of bays along the coast, and male and female dolphins. Surveys were conducted from south of the Savannah River to north of Ossabaw Sound in Georgia where foraging behaviors were classified. Multivariate Generalized Additive Models were used to test correlations of behaviors to dolphin group size, depth, salinity, temperature, creek width, and tide. Sightings with headstands (p = 0.009), hard stops (p = 0.019), chasing (p = 0.004), mudbank whacking (p < 0.001), herding/circling (p = 0.024), and strand feeding (p = 0.006) were correlated with shallow water or small creeks. Sightings with kerplunking (p = 0.031), mudbank whacking (p = 0.001), strand feeding (p = 0.003), and herding/circling (p = 0.026) were significantly correlated with low tide. The results of the Savannah, Georgia study were the first to characterize foraging behaviors in this area and demonstrate how bottlenose dolphins utilize the salt marsh estuary in terms of foraging. Studies like these are important to determine how dolphins forage efficiently and to provide background information on diet and foraging behavior for use in monitoring future impacts to dolphins in the Northwest Florida Panhandle and near Savannah, Georgia.

Cheshire stillwaters. Summary results of 1997 data Oak Mere, Betley Mere and Marbury Big

This is the Cheshire stillwaters. Summary results of 1997 data Oak Mere, Betley Mere and Marbury Big from the National Rivers Authority, June 1998. In May 1997, a Stillwaters meeting was held to discuss the way forward in stillwaters monitoring. It decided upon the establishment of a three year rolling programme, in which three stillwaters would be monitored three times a year, every third year. The stillwaters where chosen due to water quality (i.e potential polluted / sensitive waters), fisheries and ecological interests. The Still waters chosen for the first year (1997) were Oak Mere, Betley Mere and Marbury Big Mere. The surveys were aimed to produce a comprehensive study of the still water through monitoring a variety of parameters. Algal, zooplankton and water chemical samples were taken three times a year, (April, July and September). In addition, fisheries surveys were taken in July and marginal invertebrate surveys taken in September.

Cheshire stillwaters. Summary results of September 1998

This is the Cheshire stillwaters. Summary results of September 1998 from the Environment Agency, 1998. In May 1997, a Stillwaters meeting was held to discuss the way forward in stillwaters monitoring. It decided upon the establishment of a three year rolling programme, in which three stillwaters would be monitored three times a year, every third year. Oak Mere, Tatton Park Mere and Hatch Mere were surveyed between the 29th - 30th September as part of the three year rolling programme. Betley Mere, Petty Pool, Scotman's Flash and Pearson's Flash were also surveyed. Surveys also included algal and zooplankton samples, which are to be analysed by APEM and presented in the end of year report. The section on results contains information about water column profile; and nutrients (chlorophyll a; Phaeophytin; Nitrate and Nitrite; Ammonia; ortho-Phosphate; Silicate; and total Phosphorus).

Stillwaters monitoring programme summary results 2000

This is the Stillwaters monitoring programme summary results 2000 from the Environment Agency. In May 1997, a Stillwaters meeting was held to discuss the way forward in stillwaters monitoring. It decided upon the establishment of a three year rolling programme, in which three stillwaters would be monitored three times a year, every third year. During 2000, stillwaters monitored for the fourth year of the Stillwaters Monitoring Programme were Hatch Mere, Marbury Big Mere, Comber Mere, Tabley Mere, Tatton Mere and Melchett Mere. Algal, zooplankton and water chemical samples were taken on all meres. Surveys of Tabley Mere and Comber Mere continued on from last year when water quality concerns were highlighted. Continuous monitoring in Oak Mere, including water level data continued in 2000. Fish surveys were carried out in Tatton Mere and Comber Mere. Tabley Mere survey was abandoned due to the awkward bathymetry of the mere. No invertebrate samples were taken in 2000 due to lack of resources.

Cheshire stillwaters summary results 1999

This is the Cheshire stillwaters summary results 1999 from the Environment Agency North West. In May 1997, a Stillwaters meeting was held to discuss the way forward in stillwaters monitoring. It decided upon the establishment of a three year rolling programme, in which three stillwaters would be monitored three times a year, every third year. During 1999, stillwaters monitored for the third year of the Stillwaters Monitoring Programme were Tabley Mere, Comber Mere and Norbury Mere. Surveys of Petty Pool and Betley Mere continued for a second and third year respectively after water quality concerns were highlighted in previous end-of-year reports. Oak Mere was also surveyed for the third year running due to its unusual ecology. This year, the variety of parameters monitored was limited to algal, zooplankton and water chemical samples. Fisheries and marginal invertebrate surveys were not completed due to lack of resources.

Stillwaters monitoring programme. Summary results 2001 and 2002

This is the Stillwaters monitoring programme. Summary results 2001 and 2002 from the Environment Agency North West. Until January 2001 the South Area Stillwaters Sampling Programme consisted of a rolling programme where five to six stillwaters were sampled three times a year (spring, summer and autumn). However, this method was not yielding the water quality information required for long term monitoring. Local weather conditions influence short-term water quality events, e.g. algal blooms, nutrient consumption, stratification, super-saturation etc, so results from one day sampling could only be regarded as individual ‘spot’ samples. Therefore year-on-year comparisons could not be made. It was decided that long-term water quality monitoring of the stillwaters would benefit more from sampling nutrient abundance over winter months. This would give an insight into the carry-over of nutrients available for algal growth the following year and so year-on-year productivity could be assessed. Survey results shown in this report were from: The Mere, Rostherne Mere, Melchett Mere, Tabley Mere, Tatton Mere, Hatchmere, Oak Mere, Black Lake, Chapel Mere, Bar Mere, Oss Mere, Marbury Big Mere, Comber Mere and Betley Mere.

Stillwaters monitoring programme.Summary results 2003 and 2004

This is the Stillwaters monitoring programme. Summary results 2003 and 2004 from the Environment Agency North West. This report is focuses in The Winter Monitoring of Stillwaters Programme, which began in January 2001 with the aim of gathering long term data on nutrient abundance over winter months. This allows assessment of nutrient ‘carry-over’ available for algal growth in the following year, plus year-on-year productivity. 14 stillwaters are monitored each year. The environmental issues associated with each Stillwater are summarised in the table below. Bank-side water samples are taken for nutrients (N, P and S) and chlorophyll. A YSI multi-parameter sonde measures temperature, pH, specific conductivity and dissolved oxygen (% saturation). Survey results shown in this report came from: Oak Mere and Bar mere.

Cheshire stillwaters summary results 1999 of different Meres

This is the Cheshire Stillwaters Summary results of 1999 produced by the Environment Agency on February 2000. The report highlights the water quality concerns of those areas surveyed by the Stillwaters Monitoring Programme: Oak Mere, Betley Mere, Petty Pool, Tabley Mere, Comber Mere and Norbury Mere. The report contains divided by area sections on physic-chemical characteristics and water chemistry, Algal and Zooplankton surveys, Discussion, Planned Surveys 2000 and survey maps. 1999 data from Petty Pool, Betley Mere, Comber Mere and Tabley Mere were classified by the trophic status of each stillwater as hyper-eutrophic/ eutrophic, and Oak Mere were classified as mesotrophic/ eutrophic.

Effects of water quality in Bassenthwaite Lake on anglers' catches of salmon and sea-trout in the River Derwent

This is the report on the Effects of Water Quality in the Bassenthwaite Lake on Anglers Catches of Salmon and Sea-trout in the River Derwent April 1993 by the Institute of Freshwater Ecology. An analysis of the catch statistics for salmon and sea-trout in the Rivers Derwent and Cocker was undertaken in relation to available information on the algal water quality in Bassenthwaite Lake to test the hypothesis that poor catch returns were associated with a deterioration of water quality within the lake. Analysis of the catch statistics failed to reveal any correlation between water quality and catch returns for either species of fish and it is concluded that any water deterioration in Bassenthwaite Lake has not caused any major damage to the salmon and sea trout fisheries of the Derwent/Cocker system. This conclusion is supported by the analysis of the Windermere/Leven and Crake system, where no correlation could be found between lake water quality and downstream catches of migratory salmonid fish. However, the possibility still exists and such an effect might be detected by further field work on the macroinvertebrates and on the composition of potential salmonid spawning in the area.

Annual report 2006 - North Pacific Marine Science Organization (PICES). Fifteenth meeting, Yokohama, Japan, October 13-22, 2006

◾ Report of Opening Session (p. 1) ◾ Report of Governing Council (p. 15) ◾ Report of the Finance and Administration Committee (p. 47) ◾ Reports of Science Board and Committees: Science Board Inter-sessional Meeting (p. 63); Science Board (p. 73); Biological Oceanography Committee (p. 87); Fishery Science Committee (p. 95); Marine Environmental Quality Committee (p. 105); MONITOR Technical Committee (p. 115); Physical Oceanography and Climate Committee (p. 125); Technical Committee on Data Exchange (p. 133) ◾ Reports of Sections, Working and Study Groups: Section on Carbon and Climate (p. 139); Section on Ecology of Harmful Algal Blooms in the North Pacific (p. 143); Working Group 18 on Mariculture in the 21st Century - The Intersection Between Ecology, Socio-economics and Production (p. 147); Working Group 19 on Ecosystem-Based Management Science and its Application to the North Pacific (p. 151); Working Group 20 on Evaluations of Climate Change Projections (p. 157); Working Group 21 on Non-indigenous Aquatic Species (p. 159); Study Group to Develop a Strategy for GOOS (p. 165) ◾ Reports of the Climate Change and Carrying Capacity Scientific Program: Implementation Panel on the CCCC Program (p. 169); CFAME Task Team (p. 175); MODEL Task Team (p. 181) ◾ Reports of Advisory Panels: Advisory Panel for a CREAMS/PICES Program in East Asian Marginal Seas (p. 187); Advisory Panel on Continuous Plankton Recorder Survey in the North Pacific (p. 193); Advisory Panel on Iron Fertilization Experiment in the Subarctic Pacific Ocean (p. 197); Advisory Panel on Marine Birds and Mammals (p. 201); Advisory Panel on Micronekton Sampling Inter-calibration Experiment (p. 205) ◾ Summary of Scientific Sessions and Workshops (p. 209) ◾ Membership List (p. 259) ◾ List of Participants (p. 277) ◾ List of PICES Acronyms (p. 301) ◾ List of Acronyms (p. 303)

Annual report 2007 - North Pacific Marine Science Organization (PICES). Sixteenth meeting, Victoria, Canada, October 26-November 5, 2007

Report of Opening Session (p. 1). Report of Governing Council (p. 15). Report of the Finance and Administration Committee (p. 65). Reports of Science Board and Committees: Science Board Inter-Sessional Meeting (p. 83); Science Board (p. 93); Biological Oceanography Committee (p. 105); Fishery Science Committee (p. 117); Marine Environmental Quality Committee (p. 129); Physical Oceanography and Climate Committee (p. 139); Technical Committee on Data Exchange (p. 145); Technical Committee on Monitoring (p. 153). Reports of Sections, Working and Study Groups: Section on Carbon and Climate (p. 161); Section on Ecology of Harmful Algal Blooms in the North Pacific (p. 167); Working Group 19 on Ecosystem-based Management Science and its Application to the North Pacific (p. 173); Working Group 20 on Evaluations of Climate Change Projections (p. 179); Working Group 21 on Non-indigenous Aquatic Species (p. 183); Study Group to Develop a Strategy for GOOS (p. 193); Study Group on Ecosystem Status Reporting (p. 203); Study Group on Marine Aquaculture and Ranching in the PICES Region (p. 213); Study Group on Scientific Cooperation between PICES and Non-member Countries (p. 225). Reports of the Climate Change and Carrying Capacity Program: Implementation Panel on the CCCC Program (p. 229); CFAME Task Team (p. 235); MODEL Task Team (p. 241). Reports of Advisory Panels: Advisory Panel for a CREAMS/PICES Program in East Asian Marginal Seas (p. 249); Advisory Panel on Continuous Plankton Recorder Survey in the North Pacific (p. 253); Advisory Panel on Iron Fertilization Experiment in the Subarctic Pacific Ocean (p. 255); Advisory Panel on Marine Birds and Mammals (p. 261); Advisory Panel on Micronekton Sampling Inter-calibration Experiment (p. 265). 2007 Review of PICES Publication Program (p. 269). Guidelines for PICES Temporary Expert Groups (p. 297). Summary of Scientific Sessions and Workshops (p. 313). Report of the ICES/PICES Conference for Early Career Scientists (p. 355). Membership (p. 367). Participants (p. 387). PICES Acronyms (p. 413). Acronyms (p. 415).

The Southeast Bering Sea Ecosystem: implications for marine resource management (Final Report: Southeast Bering Sea Carrying Capacity).

Southeast Bering Sea Carrying Capacity (SEBSCC, 1996–2002) was a NOAA Coastal Ocean Program project that investigated the marine ecosystem of the southeastern Bering Sea. SEBSCC was co-managed by the University of Alaska Fairbanks, NOAA Alaska Fisheries Science Center, and NOAA Pacific Marine Environmental Laboratory. Project goals were to understand the changing physical environment and its relationship to the biota of the region, to relate that understanding to natural variations in year-class strength of walleye pollock (Theragra chalcogramma), and to improve the flow of ecosystem information to fishery managers. In addition to SEBSCC, the Inner Front study (1997–2000), supported by the National Science Foundation (Prolonged Production and Trophic Transfer to Predators: Processes at the Inner Front of the S.E. Bering Sea), was active in the southeastern Bering Sea from 1997 to 1999. The SEBSCC and Inner Front studies were complementary. SEBSCC focused on the middle and outer shelf. Inner Front worked the middle and inner shelf. Collaboration between investigators in the two programs was strong, and the joint results yielded a substantially increased understanding of the regional ecosystem. SEBSCC focused on four central scientific issues: (1) How does climate variability influence the marine ecosystem of the Bering Sea? (2) What determines the timing, amount, and fate of primary and secondary production? (3) How do oceanographic conditions on the shelf influence distributions of fish and other species? (4) What limits the growth of fish populations on the eastern Bering Sea shelf? Underlying these broad questions was a narrower focus on walleye pollock, particularly a desire to understand ecological factors that affect year-class strength and the ability to predict the potential of a year class at the earliest possible time. The Inner Front program focused on the role of the structural front between the well-mixed waters of the coastal domain and the two-layer system of the middle domain. Of special interest was the potential for prolonged post-spring-bloom production at the front and its role in supporting upper trophic level organisms such as juvenile pollock and seabirds. Of concern to both programs was the role of interannual and longer-term variability in marine climates and their effects on the function of sub-arctic marine ecosystems and their ability to support upper trophic level organisms.

Characterization of Hypoxia: Topic I Report for the Integrated Assessment on Hypoxia in the Gulf of Mexico

Nutrient overenrichment from human activities is one of the major stresses affecting coastal ecosystems. There is increasing concern in many areas around the world that an oversupply of nutrients from multiple sources is having pervasive ecological effects on shallow coastal and estuarine areas. These effects include reduced light penetration, loss of aquatic habitat, harmfid algal blooms, a decrease in dissolved oxygen (or hypoxia), and impacts on living resources. The largest zone of oxygen-depleted coastal waters in the United States, and the entire western Atlantic Ocean, is found in the northern Gulf of Mexico on the Louisiana-Texas continental shelf. This zone is influenced by the freshwater discharge and nutrient flux of the Mississippi River system. This report describes the seasonal, interannual, and long-term variability in hypoxia in the northern Gulf of Mexico and its relationship to nutrient loading. It also documents the relative roles of natural and human-induced factors in determining the size and duration of the hypoxic zone.

Increased toxicity of Karenia brevis during phosphate limited growth: ecological and evolutionary implications

Karenia brevis is the dominant toxic red tide algal species in the Gulf of Mexico. It produces potent neurotoxins (brevetoxins [PbTxs]), which negatively impact human and animal health, local economies, and ecosystem function. Field measurements have shown that cellular brevetoxin contents vary from 1–68 pg/cell but the source of this variability is uncertain. Increases in cellular toxicity caused by nutrient-limitation and inter-strain differences have been observed in many algal species. This study examined the effect of P-limitation of growth rate on cellular toxin concentrations in five Karenia brevis strains from different geographic locations. Phosphorous was selected because of evidence for regional P-limitation of algal growth in the Gulf of Mexico. Depending on the isolate, P-limited cells had 2.3- to 7.3-fold higher PbTx per cell than P-replete cells. The percent of cellular carbon associated with brevetoxins (%C-PbTx) was ~ 0.7 to 2.1% in P-replete cells, but increased to 1.6–5% under P-limitation. Because PbTxs are potent anti-grazing compounds, this increased investment in PbTxs should enhance cellular survival during periods of nutrient-limited growth. The %C-PbTx was inversely related to the specific growth rate in both the nutrient-replete and P-limited cultures of all strains. This inverse relationship is consistent with an evolutionary tradeoff between carbon investment in PbTxs and other grazing defenses, and C investment in growth and reproduction. In aquatic environments where nutrient supply and grazing pressure often vary on different temporal and spatial scales, this tradeoff would be selectively advantageous as it would result in increased net population growth rates. The variation in PbTx/cell values observed in this study can account for the range of values observed in the field, including the highest values, which are not observed under N-limitation. These results suggest P-limitation is an important factor regulating cellular toxicity and adverse impacts during at least some K. brevis blooms.

Global transcriptional responses of the toxic cyanobacterium, Microcystis aeruginosa, to nitrogen stress, phosphorus stress, and growth on organic matter

Whole transcriptome shotgun sequencing (RNA-seq) was used to assess the transcriptomic response of the toxic cyanobacterium Microcystis aeruginosa during growth with low levels of dissolved inorganic nitrogen (low N), low levels of dissolved inorganic phosphorus (low P), and in the presence of high levels of high molecular weight dissolved organic matter (HMWDOM). Under low N, one third of the genome was differentially expressed, with significant increases in transcripts observed among genes within the nir operon, urea transport genes (urtBCDE), and amino acid transporters while significant decreases in transcripts were observed in genes related to photosynthesis. There was also a significant decrease in the transcription of the microcystin synthetase gene set under low N and a significant decrease in microcystin content per Microcystis cell demonstrating that N supply influences cellular toxicity. Under low P, 27% of the genome was differentially expressed. The Pho regulon was induced leading to large increases in transcript levels of the alkaline phosphatase phoX, the Pst transport system (pstABC), and the sphX gene, and transcripts of multiple sulfate transporter were also significantly more abundant. While the transcriptional response to growth on HMWDOM was smaller (5–22% of genes differentially expressed), transcripts of multiple genes specifically associated with the transport and degradation of organic compounds were significantly more abundant within HMWDOM treatments and thus may be recruited by Microcystis to utilize these substrates. Collectively, these findings provide a comprehensive understanding of the nutritional physiology of this toxic, bloom-forming cyanobacterium and the role of N in controlling microcystin synthesis.