Freeze drying of fishery products: Part V - Storage characteristics of ready-to-serve freeze dried foods

Behaviour of freeze dried ready-to-serve fish based food preparations during prolonged storage at room temp has been studied and reported in this paper. Storage life of such products under the conditions employed in this study can be reckoned in years at our ambient temps.

The influence of plating technique and incubation temperature on bacterial count from fish and fishery products

For bacterial sampling of raw unprocessed fish and frozen fishery products, spread plate method is preferable to pour plate method; incubation of plates at 30°C gives a higher count than incubation at 37°C. Analysis of variance of the data shows that sample variation between different types of fishes is highly significant whereas the variations between triplicate plates are not significant at 5 % level.

Pickled products from green mussel

Methods of preparing different types of delicious ready to serve pickled products from green mussel (Perna viridis) and a comparative study of their storage characteristics are reported. Of the three types of products, namely, dried and pickled, fried and pickled and light smoked and pickled, the last one had the best shelf life. The optimum conditions of drying and smoking for preparing such type of pickles are also reported.

Observations on the parasites and associates of wood boring molluscs and crustaceans of the south-west coast of India

An interesting assemblage of commensalic organisms ranging from Protozoa to Arthropoda has been identified from the wood boring animals (molluscs and crustaceans) from the south-west coast of India. Certain aspects of the general biology of the associated ciliates such as the nature of incidence, division in relation to environmental parameters, survival outside the body of the host and reactions related to the general condition of the host are described. Results are also presented of the tolerance of the rare commensalic hydroid Eutima commensalis to different salinities of the medium.

Quality of cured fishery products from Malabar and Kanara coasts

Results of chemical, bacteriological and organoleptic quality studies of cured fishery products of commerce collected from six major fish curing centres on the west coast of India are presented. 77.12% of the samples had moisture above 35%, 97.18% showed salt content below 25% and all samples had acid insoluble ash above 1.5%. 42.32% gave standard plate counts above 10,000 and 45.77% were contaminated with 'Red' halophiles. The major defects in curing were imperfect cleaning, inadequate salting and unhygienic conditions of processing.

Vertical distribution of marine wood boring and fouling organisms from the estuarine areas of the south west coast of India

Vertical distribution of marine wood boring and fouling organisms from three different estuarine areas namely, the Ernakulam channel in the Cochin backwaters, Ayiramthengu in the Kayamkulam Lake and Neendakara in the Asthamudi Lake during the post-monsoon, the pre-monsoon and the monsoon periods is presented. The boring organisms noticed during the present study were Martesia striata, Teredo furcifera, Nausitora hedleyi and Sphaeroma terebrans. The dominant fouling organisms were Balanus amphitrite amphitrite, calcareous worms and Modiolus sp. Algae and diatoms were very common on the sub-tidal panels during the monsoon. The incidence of Teredo, Nausitora and calcareous tube worms were significantly high on the bottom panels. Sphaeroma, Balanus and Modiolus occurred in greater numbers on the intertidal panels.

Sampling plans for pre-packed fish and fish products

An outline is given of a sampling plan for prepacked fish products based on the net weight of the container, which may be used to make the inspection of fish and fish products easier and more effective.

Selection of suitable diluents for bacteriological examination of fishery products

For raw, iced and frozen samples of fish and prawn, significant difference was observed in total plate counts done with various diluents, the significance level ranging from 5% to 0.1%. For raw fish, N-saline, seawater and quarter strength Ringers' solution gave maximum total plate counts. In the case of iced-fish, n-saline yielded highest total plate counts. For frozen samples, however, peptone water and n-saline gave good recoveries. Trials with suitable combinations of diluents showed that though some of them were as good as the control, namely n-saline, none were superior in any way.

Antibiotic sensitivity of Staphylococcus aureus strains isolated from fishery products

During the course of an investigation on the incidence of Staphylococcus aureus contamination in frozen crab meat, frozen prawns and dried fishes from Cochin, 116 strains of S. aureus were isolated. The sensitivity of the isolated strains towards nine antibiotics showed that all the S. aureus strains were sensitive to kanamycin and streptomycin (100%). Sensitivity to other antibiotics like chloramphenicol, polymyxin-B, erythromycin, tetracycline, neomycin, penicillin and ampicillin were shown by 98.28, 93.10, 87.93, 68.10, 67.24, 56.90 and 55.17 percent of the isolates respectively.

Quality of certain ready-to-eat fish products of the domestic trade

Quality of 181 samples of ready-to-eat fish products comprising fried fish, fish curry and fish/prawn pickles collected from Cochin and Calicut were studied. Salmonella was absent in all the samples. V. cholerae was tested in the samples collected at Cochin and was absent in all the cases. Coliforms, E. coli, faecal streptococci and coagulase-positive staphylococci were present in some of the samples studied. The study indicated the necessity to improve the sanitary and hygienic conditions of the hotels engaged in the preparation of these products. The study further indicated that fried fish and fish curry shall not be served after 6 hours of their preparation. Added care is to be exercised in the selection of shrimps and fish for the preparation of pickles.

Occurrence of enterotoxigenic staphylococci in frozen fishery products

Sixty six samples of frozen fishery products consisting of frozen crab meat, frozen prawns, frozen cuttlefish and frozen mussel collected from nine cold stores situated in and around Cochin city were examined for coagulase positive staphylococci. Forty eight samples (72.72%) revealed the presence of coagulase positive staphylococci, which were then tested for enterotoxigenity, employing cellophane-over-agar method for toxin production and optimum-sensitivity-plate (OSP) method for toxin detection. Seventy seven out of one hundred and two (75.49%) coagulase positive staphylococcal isolates from the above products produced enterotoxins A, B, C, D and E either singly or in combinations. Enterotoxin A and D were detected more often than others. The occurrence of enterotoxigenic staphylococci in frozen fishery product is reported for the first time in this country.

Incidence of arthropods in dried fish products

Arthropods have been recorded from various types of insect infested dried fish products stored in the laboratory. They have been identified as Suidesia nesbetti Hughes (Acaridae) infesting dried anchovies and dried mussel, Dermestes ater Dermestidae coleoptera) attacking wet cured sardines and smoked catfish and Stegobium panicium infesting smoked catfish and dried mussel. Incidences of Stegobium panicium in dry fish products and Suidesia nesbetti in dried mussel has been recorded for the first time.

Incidence and low temperature survival of coagulase positive staphylococci in fishery products

Coagulase-positive staphylococci was found to be absent in all the frozen samples of lobsters, cuttle fish, cat fish, seer fish and red snapper examined. Coagulase-positive staphylococci were present in 38% of the cooked frozen shrimps and only 16% of the samples had staphylococci count more than 100/g. In the case of headless, peeled and deveined, peeled undeveined shrimps, the incidence of the organism was 6, 12 and 16% respectively. The study indicated that the incidence of coagulase-positive staphylococci is not a serious problem in frozen fishery products processed in this country. There was remarkable difference in the rate of destruction of coagulase-positive staphylococci in raw and cooked shrimps during freezing and frozen storage.

Levels of cadmium in seafood products

The concentrations of total cadmium were determined in 448 samples of processed fishery products comprising crustaceans, molluscs, marine fish and brackish water fish. Shrimp product, the major export item, contained on average 0.122 ppm cadmium and 20% of crustaceans analysed did not contain cadmium on detectable level. Average content of cadmium in 68 marine fish of 9 species was 0.153 ppm and 35% of the samples did not contain the element. Of the total number of crustaceans analysed only 2% showed a level > 0.5 ppm. All the 174 samples of molluscan products, except 3 canned oysters, showed cadmium concentration far below the limit allowed. The average cadmium content in molluscan products was 0.552 ppm.

Incidence and low temperature survival of Salmonella in fishery products

Salmonella was isolated from 12% of PD shrimps, 10% of HL shrimps, 14% of PUD shrimps, 17% of lobsters, 14% of cuttle fish, 25% of cat fish and 20% of seer fish (all frozen) tested. One percent of the fish meal, 4% of dried non-penaeid prawn and 23% of sea beach sand showed incidence of the organism. Salmonella was also isolated from 2 and 4% of the swab samples of utensil surfaces and the floor surface of the processing hall respectively as well as from 1% of the process water tested. All the serotypes of Salmonella tested were resistant to freezing at -40°C, but during subsequent storage at -20°C, there was some difference between the serotypes with regard to their viability, S. paratyphi B being the most resistant which survived up to 9 months while S. saintpaul the least resistant having survival up to 5 months only.