Monitoring Hawaii’s marine protected areas: examining spatial and temporal trends using a seascape approach

Hawaii’s coastal marine resources have declined dramatically over the past 100 years due to multiple anthropogenic stressors including overfishing, coastal development, pollution, overuse, invasive species and climate change. It is now becoming evident that ecosystem-based management, in the form of marine protected areas (MPAs), is necessary to conserve biodiversity, maintain viable fisheries, and deliver a broad suite of ecosystem services. Over the past four decades, Hawaii has developed a system of MPAs to conserve and replenish marine resources around the state. These Marine Life Conservation Districts (MLCDs) vary in size, habitat quality, and management regimes, providing an excellent opportunity to test hypotheses concerning MPA design and function using multiple discreet sampling units. NOAA/NOS/NCCOS/Center for Coastal Monitoring and Assessment’s Biogeography Branch used digital benthic habitat maps coupled with comprehensive ecological studies between 2002 and 2004 to evaluate the efficacy of all existing MLCDs using a spatially-explicit stratified random sampling design. The results from this work have shown that areas fully protected from fishing had higher fish biomass, larger overall fish size, and higher biodiversity than adjacent areas of similar habitat quality. Other key findings demonstrated that top predators and other important fisheries species were more abundant and larger in the MPAs, illustrating the effectiveness of these closures in conserving these populations. Habitat complexity, protected area size and habitat diversity were the major factors in determining effectiveness among MPAs.

A model for assessing the likelihood of self-sustaining populations resulting from commercial production of triploid Suminoe oysters (Crassostrea ariakensis) in Chesapeake Bay

Culture of a non-native species, such as the Suminoe oyster (Crassostrea ariakensis), could offset the harvest of the declining native eastern oyster (Crassostrea virginica) fishery in Chesapeake Bay. Because of possible ecological impacts from introducing a fertile non-native species, introduction of sterile triploid oysters has been proposed. However, recent data show that a small percentage of triploid individuals progressively revert toward diploidy, introducing the possibility that Suminoe oysters might establish self-sustaining populations. To assess the risk of Suminoe oyster populations becoming established in Chesapeake Bay, a demographic population model was developed. Parameters modeled were salinity, stocking density, reversion rate, reproductive potential, natural and harvest-induced mortality, growth rates, and effects of various management strategies, including harvest strategies. The probability of a Suminoe oyster population becoming self-sustaining decreased in the model when oysters are grown at low salinity sites, certainty of harvest is high, mini-mum shell length-at-harvest is small, and stocking density is low. From the results of the model, we suggest adopting the proposed management strategies shown by the model to decrease the probability of a Suminoe oyster population becoming self-sustaining. Policy makers and fishery managers can use the model to predict potential outcomes of policy decisions, supporting the ability to make science-based policy decisions about the proposed introduction of triploid Suminoe oysters into the Chesapeake Bay.

Intermingling and seasonal migrations of Greenland halibut (Reinhardtius hippoglossoides) populations determined from tagging studies

A total of 7244 Greenland halibut (Reinhardtius hippoglossoides, Walbaum) were tagged in Greenland waters between 1986 and 1998 to increase information on stock delineations, to clarify migration routes, and to describe the seasonal movements of fjord populations. At present 517 recaptured Greenland halibut have been recorded. For Greenland halibut released in Davis Strait, Baffin Bay, and the fjords of southwestern and eastern Greenland, a substantial portion of recovered fish demonstrated migratory behavior, up to 2500 km, primarily to Denmark Strait between Greenland and Iceland. The recaptured fish provided evidence of intermingling between the population in Denmark Strait and the populations in Davis Strait and the southwest Greenland fjords. These observations support those of other studies that indicate that Greenland halibut inhabiting Davis Strait and the fjords of southwestern and eastern Greenland originate in the spawning grounds west of Iceland. The high mobility of offshore Greenland halibut within Baffin Bay and Davis Strait suggests that Greenland halibut migrate extensively between feeding and spawning areas. Greenland halibut in the fjords of northwestern Greenland appear to be resident in behavior and do not intermingle with offshore or more southerly inshore populations. A seasonal pattern in the recovery of these fish indicates that Greenland halibut aggregate in the inner part of fjords during the second half of the year (when inshore waters are not covered with ice).

An examination of spatial and temporal genetic variation in walleye pollock (Theragra chalcogramma) using allozyme, mitochondrial DNA, and microsatellite data

We used allozyme, microsatellite, and mitochondrial DNA (mtDNA) data to test for spatial and interannual genetic diversity in wall-eye pollock (Theragra chalcogramma) from six spawning aggregations representing three geographic regions: Gulf of Alaska, eastern Bering Sea, and eastern Kamchatka. Interpopulation genetic diversity was evident primarily from the mtDNA and two allozyme loci (SOD-2*, MPI*). Permutation tests ˆindicated that FST values for most allozyme and microsatellite loci were not significantly greater than zero. The microsatellite results suggested that high locus polymorphism may not be a reliable indicator of power for detecting population differentiation in walleye pollock. The fact that mtDNA revealed population structure and most nuclear loci did not suggests that the effective size of most walleye pollock populations is large (genetic drift is weak) and migration is a relatively strong homogenizing force. The allozymes and mtDNA provided mostly concordant estimates of patterns of spatial genetic variation. These data showed significant genetic variation between North American and Asian populations. In addition, two spawning aggregations in the Gulf of Alaska, in Prince William Sound, and off Middleton Island, appeared genetically distinct from walleye pollock spawning in the Shelikof Strait and may merit management as a distinct stock. Finally, we found evidence of interannual genetic variation in two of three North American spawning aggregations, similar in magnitude to the spatial variation among North American walleye pol-lock. We suggest that interannual genetic variation in walleye pollock may be indicative of one or more of the following factors: highly variable reproductive success, adult philopatry, source-sink metapopulation structure, and intraannual variation (days) in spawning timing among genetically distinct but spatially identical spawning aggregates.

Offshore coho salmon populations near the Pacific Northwest and large-scale atmospheric events

EXTRACT (SEE PDF FOR FULL ABSTRACT): Catch of coho salmon off the coast of Washington and Oregon since 1925 appears to be related to large-scale events in the atmosphere, which in turn affect ocean currents and coastal upwelling intensities in the northeastern Pacific. At least two time scales of variations can be identified. The first is that of the El Nino/Southern Oscillation phenomenon giving rise to an irregular cycle of between 3 to 7 years. ... The second time scale of variation seems to have a periodicity of about 20 years, although this is based on a limited dataset. ... This paper endeavors to describe how, if real, these atmospheric/oceanic effects are integrated and might affect the salmon catch. The possibility must also be considered that the atmospheric events are symbiotically related to the oceanic events and, further, that both may be enmeshed in even longer-term variability of climate.

On the naturalness of change in marine populations

Based on a talk given before a meeting of the Society of Fisheries Technologists, India.

Allozyme variation in four hatchery populations of Thai pangas, Pangasius hypophthalmus in Bogra, Bangladesh

Genetic variation of four hatchery stocks of Thai pangas, Pangasius hypophthalmu [sic] of Bogra region, Bangladesh was studied from 1 January 2002 to 31 December 2003. Muscle samples were collected for allozyme analysis from four (Bhai-Bhai, Jahangir, Belal and Bhai-Bon) different hatchery populations. For allozyme electrophoresis, eight enzymes were used and 11 loci viz. Adh-1*, Est-1*, GJpdh-1*, Gpi-1*, Gpi-2*, Jdhp-1*, Ldh-1*, Ldh-2*, Mdh-1*, Mdh-2*and Pgm* were identified, of which three loci (Est-1*, Gpi-2*, G3pdh-1 and Pgm*) were polymorphic in all the four populations. The mean proportion of polymorphic loci per population and the mean proportion of heterozygous loci per individual was 36.36% and 13.33, respectively for all the population studied. The highest variability measured by the mean number of alleles per locus was 1.545 in Bhai-Bon hatchery population. Based on Nei's (1972) genetic distance, the dendrogram (UPGMA) shows that four populations have made two clusters by D-value (D=0.043). Bhai-Bhai and Jahangir hatchery populations have made cluster-I, and Belal Uddin and Bhai-Bon hatchery populations formed cluster-II. Among the four populations, BhaiBhai and Jahangir hatchery populations were differentiated from each other by the D-value of 0.013, and Belal Uddin and Bhai-Bon populations were differentiated from each other by the D-value of 0.002, which suggests that the four populations may be fallen into the local population or race.

Estimation of RNA/DNA ratio in two north-Indian natural populations of mahseer (Tor tor) and its relationship with growth and hydrobiology

Samples of Tor tor were collected from Bari Reservoir of Udaipur and Narmada River at Hoshangabad (India), in the months of July and November 2005, respectively. Twenty-five samples were collected from each location. Bari Reservoir samples ranged from 17.0 to 24.5 cm in total length and from 75 to 155 g in weight, while Narmada samples ranged from 20.0 to 42.0 cm in length and 90 to 425 g in weight. The nucleic acid content in body muscle of Tor tor and the RNA/DNA ratio were estimated. The age of fishes was estimated by the scale study method and specimens were classified into four age groups. RNA/DNA ratio showed significant linear increase with increase in weight and age till the age of three years after which, the growth rate reduced. The 1-2 year group was the only one common between the two water bodies and a comparison of RNA/DNA ratios showed higher growth rate in Bari Reservoir. The gross primary productivity was also higher in Bari Reservoir being 551 mg cmˉ³ dˉ¹ compared to 404 mg cmˉ³ dˉ¹ observed for Narmada River. The condition factor (K) was found to be higher (1.21) in the fish from the Bari Reservoir compared to those of Narmada River (1.14). The growth rate was higher in females compared to males in >100 g specimens.

Genetic analysis of east and west coast populations of Penaeus monodon from India based on random amplified polymorphic DNA

The east and west coast populations of wild Penaeus monodon in India were genetically characterized by RAPD analysis using six highly polymorphic primers reported earlier. The average genetic similarities within populations, based on profiles generated by all the six primers, were 0.828 and 0.851 for the east and west coast populations, respectively, values with individual primers ranging from 0.744 to 0.889. The average genetic similarity between populations across all the primers was 0.774. The number of bands found to be polymorphic were 38 (51.35%) and 37 (50.68%) in the east and west coast populations, respectively. Primer 5 yielded the highest level of polymorphism (63.63%) in the east coast population whereas primer 3 yielded the lowest level of polymorphism (36.36%) in the west coast population. The study reveals the existence of genetic variation in P. monodon stocks providing scope for genetic improvement through selective breeding. It also provides baseline data for future work on population structure analysis of P. monodon.

Reduction in Chaetoceros populations by furanace

One of the most promising prophylactic agents being tested to control Penaeus monodon larval diseases is furanace (6-hydroxymethyl-2 2(5-nitro-2-furyl) vinyl pyridine). To evaluate further its suitability as a chemotherapeutic agent, its effects on the population growth of Chaetoceros calcitrans, the diatom used as feed for the zoeal stages, was examined. Chaetoceros populations of uniform density (initial density in all runs: 130-141x10 SUP-3 cells /ml) were placed in nine white, circular (382 sq cm), plastic basins. The physio-chemical characteristics of the culture water were as follows: salinity, 28 . 5-30 . 0 ppt; pH, 8 . 62-8 . 72; temperature, 23-25 . 5 degree C; dissolved oxygen, 7 . 1-9 . 3 ppm; nitrate, 0 . 03-0 . 07 ppm; and ammonia, 0 . 005-0 . 03 ppm. Preweighed furanace granules were dissolved in the culture water, with resulting concentrations of 1 and 2 mg/l (3 replicates each). A set of replicates without furanace served as the control. Population counts of the diatom were taken after 2, 4, 6, 8, and 10 hr exposures. After 4 hr, the population decreased in all three levels. The population in 2 mg/l furanace showed the lowest count and that in control the highest. The population means are not statistically different from one another. The results of the study show that the furanace causes reductions in Chaetoceros population at all durations of exposure.

Species identification of family Lutjanidae and separation of populations of John’s snapper (Lutjanus johnii) in Persian Gulf and Oman Sea based on otolith shape analysis

The anatomical and morphometric (shape indices, contour descriptors and otolith weight) characterizations of sagittal otoliths were investigated in 13 species of Lutjanus spp. inhabiting the Persian Gulf. This is the first study that compares the efficiency of three different image analysis techniques for discriminating species based on the shape of the outer otolith contour, including elliptical Fourier descriptors (EFD), fast Fourier transform (FFT) and wavelet transform (WT). Sagittal otoliths of snappers are morphologically similar with some small specific variations. The use of otolith contour based on wavelets (WT) provided the best results in comparison with the two other methods based on Fourier descriptors, but only the combination of the all three methods (EFD, FFT and WT) was useful to obtain a robust classification of species. The species prediction improved when otolith weight was included. In relation to the shape indices, only the aspect ratio provided a clear grouping of species. Also, another study was carried on to test the possibility of application of shape analysis and comparing otolith contour of otoliths of Lutjanus johnii from Persian Gulf and Oman Sea to identify potential stocks. The results showed the otoliths have differences in contour shape and can be contribute to two different stocks.

The genetic diversity of Acipenser stellatus populations in the Caspian Sea was studied using microsatellite technique

A total of 361 caudal fin samples were collected from adult A. stellatus specimens caught in the north Caspian Sea, including specimens from Kazakhstan (Ural River), Russia (Volga River), Azerbaijan (Kura River), specimens caught in the south Caspian Sea including specimens from Fishery Zone 1 (from Astara to Anzali), Fishery Zone 2 (from Anzali to Ramsar), Fishery Zone 3 (from Nowshahr to Babolsar), Fishery Zone 4 (from Miyankaleh to Gomishan) as well as from specimens caught in Turkmenistan (all specimens were collected during the sturgeon stock assessment survey). About 2 g of fin tissue was removed from each caudal fin sample, stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed using 1% Agarose gel electrophoresis and Polymerase Chain Reaction (PCR) was conducted on the target DNA using 15 paired microsatellite primer. PCR products were electrophoresed on polyacrylamide gels (6%) that were stained using silver nitrate. Electrophoretic patterns and DNA bands were analyzed with BioCapt software. Allele count and frequency, genetic diversity, expected heterozygosity and observed heterozygosity allele number, and the effective allele number, genetic similarity and genetic distance, FST and RST were calculated. The Hardy Wienberg Equilibrium based on X2 and Analysis of Molecular Variance (AMOVA) at 10% confidence level was calculated using the Gene Alex software. Dendrogram for genetic distances and identities were calculated using TFPGA program for any level of the hierarchy. It is evident from the results obtained that the 15 paired primers studied, polymorphism was observed in 10 pairs in 12 loci, while one locus did not produce DNA bands. Mean allele number was 13.6. Mean observed and expected heterozygosity was 0.86 and 0.642, respectively. It was also seen that specimens from all regions were not in Hardy Wienberg Equilibrium in most of the loci (P≤0.001). Highest Fst (0.063) was observed when comparing specimens from Fishery Zone 2 and Fishery Zone 4 (Nm=3.7) and lowest FST (0.028) was observed when comparing specimens from the Volga River and those from the Ural River (8.7). Significant differences (P<0.01) were observed between RST recorded in the specimens studied. Highest genetic distance (0.604) and lowest genetic resemblance (0.547) were observed between specimens from Fishery zones 2 and 4. Lowest genetic distance (0.311) and highest genetic resemblance (0.733) was observed between specimens from Turkmenistan and specimens from Fishery zone 1. Based on the genetic dendrogeram tree derived by applying UPGMA algorithm, A. stellatus specimens from Fishery zone 2 or in other words specimens from the Sepidrud River belong to one cluster which divides into two clusters, one of which includes specimens from Fishery zones 1, 3 and 4 and specimens from Turkmenistan while the other cluster includes specimens from Ural, Volga and Kura Rivers. It is thus evident that the main population of this species belongs to the Sepidrud River. Results obtained from the present study show that at least eight different populations of A. stellatus are found in the north and south Caspian Sea, four of which are known populations including the Ural River population, the Volga River population, the Kura River population and the Sepidrud River populations. The four other populations identified belonging to Fishery zones 1, 3, and 4 and to Turkmenistan are most probably late or early spawners of the spring run and autumn run of each of the major rivers mentioned. Specific markers were also identified for each of the populations identified. The Ural River population can be identified using primers Spl-68, 54b and Spl-104, 163 170, 173, the Volga River population can be identified using primers LS-54b and Spl-104, 170, 173 113a and similarly the population from the Kura River can be identified using primers LS-34, 54b and Spl-163, 173 and that from the Sepidrud River can be identified using primers LS-19, 34, 54b and Spl-105, 113b. This study gives evidence of the presence of different populations of this species and calls for serious measures to be taken to protect the genetic stocks of these populations. Considering that the population of A. stellatus in Fishery zone 2 is an independent population of the Sepidrud River in the Gilan Province, the catch of these fishes in the region needs to be controlled and regulated in order to restore the declining stocks of this species.