Manipulation of chromosomes in fish: review of various techniques and their implications in aquaculture

Human ingenuity has made it possible to advent the chromosome manipulation techniques to produce individuals with differing genomic status in a number of fish using various causal agents such as physical shocks (temperature or hydrostatic pressure), chemical (endomitotics) and anesthetic treatments either to suppress the second meiotic division shortly after fertilization of eggs or to prevent the first mitotic division shortly prior to mitotic cleavage formation. This results in the induction of polyploidy (triploidy and tetraploidy), gynogenesis (both meiotic and mitotic leading to clonal lines) and androgenesis in fish population. The rationale for the induction of such ploidy in fish has been its potential for generating sterile individuals, rapidly inbred lines and masculinized fish, which could be of benefit to fish farming and aquaculture. In this paper, these are critically reviewed and the implication of recently developed chromosome manipulation techniques to various fin fishes is discussed.

Techniques for the transport and maintenance of scleractinian coral

Several small scleractinian coral colonies were collected from a remote reef and transferred [to] the Louisiana Universities Marine Center (LUMCON) for in vitro reproductive and larval studies. The species used here were Porites astreoides and Diploria strigosa. Colony size was ~20 cm in diameter. Colonies were brought to the surface by liftbag and stored in modified ice coolers. They were transported from Freeport, TX to Cocodrie, LA by truck for nearly 15 hours where field conditions were simulated in waiting aquaria. This document describes the techniques and equipment that were used, how to outfit such aquaria, proper handling techniques for coral colonies, and several eventualities that the mariculturist should be prepared for in undertaking this endeavor. It will hopefully prevent many mistakes from being made.

Production of triploid African catfish, Clarias gariepinus (Burchell), using chromosome manipulation techniques

An experiment was conducted to induce triploidy in African catfish, Clarias gariepinus, using heat shock and cold shock techniques. Cold shock at a temperature of 0± 1°C and 5±1°C for a duration of 15, 30, 45 and 60 min and heat shock at a temperature of 40±0.5°C and 41 ±OS C for a duration of 1, 2 and 3 min was given to induce triploidy 5 min after fertilization. Maximum percentage of triploids (91.4%) were obtained in the heat shock at a temperature of 40±0SC for a duration of 1 min whereas cold shock at 0± 1 C for a duration of 60 min yielded 90% of triploids. Chromosome analysis revealed that diploids have 54 chromosomes and triploids have 81 chromosomes. The erythrocyte measurements of the minor axis and major axis were 1.17 times larger in treated fish than in controls. The growth studies showed that the growth rate was not significantly affected in triploids.

Development of artificial breeding techniques for long-whiskered catfish, Sperata aor and giant river catfish, Sperata seenghala of Bangladesh

Sperata aor and S. seenghala are the two important native catfishes of Bangladesh but commercial farming of these species is not possible due to lack of naturally collected or artificially produced seeds for stocking. Attempts were made to develop techniques for seed production by artificial breeding and nursery-rearing of fries of these catfishes. A total of 60 S. seenghala (750-1,500 g) and 10 S. aor (600-1,000 g) broods were collected from the Brahmaputra river-basin and floodplains in Mymensingh region four months prior to their breeding season. The collected brood fishes were reared in separate earthen ponds with supplementary feeds comprising of rice bran (40%), mustard oil cake (29%), fish meal (30%) and vitamin-premix (1 %). Three experiments were conducted to optimize the hormone dose. A total of nine S. seenghala females weighing from 750 to 1,500 g were given an initial and resolving dose of 12-20 and 16-24 mg PG/kg body weight, respectively. The males weighing from 650-950 g were administered a single dose of 18-26 mg PG/kg body weight at the time of the time of administering the resolving dose to the females. The females ovulated partially and the eggs were examined under a compound microscope, but most of them were found to be less ripe or damaged. Collection of milt by stripping the males was not successful. The testes were taken out and sperm were observed to be non-motile and less developed. In view of stimulating natural propagation of S. seenghala, artificial holes (nests) were constructed in the pond bottom. Each hole was 0.7 m in diameter and 0.3 m in depth. A total of 10 holes were made and then 10 pairs of S. seenghala breeders (800-1,200 g) were stocked in the pond. In mid February, 3,000 fry of S. seenghala with a mean length of 4.60 cm and weight of 0.36 g were collected by repeated netting followed by drying of the pond. The fry were then stocked in a nursery pond and fed with commercial feed (SABINCO starter-1). The average length and weight of the fingerlings were 9.01 cm and 3.95 g, respectively and the estimated survival was 60% after two months of rearing. S. aor did not respond to natural spawning. Further study is essential to develop techniques for their successful artificial and natural breeding.

Mapping of the Indian fisheries growth rate and fish consumption through GIS

In the present study, Indian fisheries growth rate and fish consumption have been analyzed through GIS mapping. The analyses were based on the state-level fisheries data of India collected from the secondary sources. Accordingly, the paper contains one thematic map containing two layers. To achieve this, all the data have been brought into a tabular form through Microsoft Excel and then joined to Map Info Professional Version 8.0 GIS software with digitized map of India for further analysis to generate thematic maps. In this thematic map, the first Jayer represents the growth-rate of fish production for the period 1990-2004 and the second layer represents fish consumption for the year 2003. The thematic map represented in graphic form presents inland, marine and total growth rates, and also the rural and urban fish consumption at the state levels. This study will be useful to fish traders, planners, researchers and administrators in fisheries policy formulation for sustainable development.

Seed production and culture techniques of Genetically Improved Farmed Tilapia (GIFT) in brackishwater environment

Effects of different levels of salinity on survival, growth and gonadal development of Genetically Improved Farmed Tilapia (GIFT) were studied under laboratory conditions in glass aquarium, for a period of ten weeks. The initial individual size of the GIFT was 20.23±4.45 and the salinity levels tested were 0, 5, 10, 15 and 20 ppt. The highest survival of 87.5% was found in 0 ppt and the lowest 60.5% in 20 ppt. Though the survival decreased progressively with increased salinity, there were no significant differences (P>0.05) among 0, 5, and 10 ppt. Similar to what has been observed in survival, the specific growth rate (SGR %/day) also decreased as of 1.30, 1.24, 1.08, 0.90 and 0.71, respectively, with the increased salinity of 0, 5, 10, 15 and 20 ppt. The gonadal development was highest in 0 ppt with a GSI value of 3.75 and lowest of 2.01 in 20 ppt. In the second experiment, gonadal development and seed production performance of GIFT in brackishwater condition were investigated for a period of three months. Each of the three fine meshed hapas of 20 square meters made from nylon net was placed in a freshwater (0 ppt) and in a brackish water (10-15 ppt) pond of the Brackishwater Station (BS). GIFT of 65 g average weight from a single cohort were stocked into three hapas at a rate of 2 per m. The male vs female ratio was 1:3. The development of gonad was faster with the higher gonadosomatic index (GSI %) of 3.85 % in freshwater condition than that of 2.73 % in brackish water. Within three months of the study period, a total of 70,510 and 44,250 GIFT fry were produced respectively, in freshwater and brackishwater conditions. Finally under third experiment, a participatory on-farm trial was carried out to evaluate the production performance of GIFT in monoculture and in polyculture with silver barb in coastal freshwater pond conditions. Nine ponds were selected for three treatment combinations of GIFT monoculture (T1), GIFT and silver barb polyculture (T2), and silver barb monoculture (T3). The ponds have been stocked in April, 05 at a density of 25,000 fry per ha. Fishes were fed with rice bran at the rate of 6% bw per day. In one month culture period, GIFT attained an average weight of 16.27 g in monoculture and 17.23 g in polyculture, against an average stocking weight of 0.37 g. Silver barb reached an average weight of 16.62 g in polyculture with GIFT and 10.01 g in monoculture, against an average stocking weight of 3.79 g.

Suitable sites for sustainable aquaculture development in Mymensingh, Bangladesh: A GIS methodological perspective

A GIS study was carried out to find out the sites suitable for aquaculture in Mymensingh region. A number of criteria were selected for GIS modeling followed by the approach of Kapetsky (1994). The criteria were developed from a range of existing data sources such as, surface and underground water level, availability of feed ingredients and animal wastes, sources of fish fry, market facilities, extension support and communication facilities for aquaculture development. The data were located, collected and compiled from different GOs and NGOs located in the region along with primary data from the field survey wherever necessary and then prepared for computer analyses. Using the database, a series of GIS models were developed in order to ascertain and prioritize the most suitable areas for aquaculture development in the region. Out of total 407,528 ha available lands in the region, the study identified 99,415 ha very suitable and 302,754 ha moderately suitable for aquaculture promotion. Result of the study is indicative to the modeling power of GIS for aquaculture application and could be used to refine the models in future, particularly if it is supported with further detail field data. To get a more concrete and complete model, detailed study should be made available on the availability of mustard oil cake, rice bran, wheat bran, and usable animal and urban wastes that could be used as low-cost feed for sustainable aquaculture.

Seed production and culture techniques of climbing perch, Anabas testudineus

Six treatments each with 12 replications designed to optimize the dose of inducing agent PG to achieve fertilization and hatching success of climbing perch, Anabas testudineus were tested. The females were given single injection of 7-12 mg PG/kg body weight and the males were given 4 mg PG/kg body weight. Fertilization and hatching rate varied from 67±4.55% to 66±3.0% and 59±4.88% to 57±6.21% for the doses of 10, 11 and 12 mg PG/kg of body weight, respectively. The hormone dose had significant (P<0.05) effect on fertilization and hatching. Six mini shallow cisterns (570 cm x 105 cm) were used to investigate the efficacy of zooplankton and Artemia nauplii as feed for spawn rearing. Three-day old spawns were stocked in six mini shallow cisterns at a stocking density of 100 individuals/L of water. Two treatments each with three replications were used to develop culture technique of the climbing perch. In case of treatment-1, the spawns were fed with Artemia nauplii three times daily, while in treatment-2, zooplankton were used as feed in the same manner as in treatment-1. After 14 days of rearing, mean final weight of the fry of treatments-1 and 2 were 95.55±6.71 and 57.69±5.40 mg, respectively. In treatment-1, spawn fed with Artemia nauplii showed significantly (P<0.05) higher mean weight than the spawn fed with zooplankton (treatment 2).

Spiny lobsters and their fishing techniques

Scores of publications on spiny lobsters and their fishing techniques are available from various parts of the world. A variety of fishing gears which vary in design and operation are employed for exploiting lobsters. A review of the work carried out on spiny lobsters with special reference to their distribution, fishing gear, fishing methods, baits and crafts in India, Sri Lanka, United States, Australia, South Africa, United Kingdom, Ireland and Portugal based on selected literature are considered and discussed.

Ecological models and GIS maps of macroalgae, as bioindicator for ecological status of Hormozgan rocky shores

In this study a total of 75 species were identified, from which 17 species, 9 genes and 6 families; belonged to Green Algae, 18 species, 7 genes and 4 families; belonged to Brown Algae, and 40 species, 18 genes and 11 families; belonged to Red Algae. From total times spent for sampling, it was determined that at lengeh harbor with 6 species, had the lowest diversity of green algae. The species diversity of brown algae at Michael location with 10 species each; had the highest, and Tahooneh location with 5 species; had the lowest species diversity. Species diversity of red algae at Michael location with 28 species; had the highest, and Sayeh Khosh location with 13 species; had the lowest diversity. From all locations where sampling took place, the highest species diversity regarding Time and Space for all three groups of algae; were associated to Late February (20th. Feb. ), and late March(20th. March). Coverage data of macroalgae and Ecological Evaluation Index indicate a high level of eutrophication for the Saieh khosh, and Bostaneh, They are classified as zones with a bad and poor ecological status. It has been proved that concentrations of biogenic elements and phytoplankton blooming are higher in these zones. The best values of the estimated metrics at Tahooneh and Michaeil could be explained with the good ecological conditions in that zone and the absence of pollution sources close to that transect . The values of abundance of macroalgae and Ecological Evaluation Index indicate a moderate ecological conditions for the Koohin, Lengeh and Chirooieh.

Broodstock management: an integral part of hatchery techniques

The paper presents bighead carp broodstock management, an integral part of hatchery techniques as it will determine the quality of resulting fry and fingerlings. Proper methods on the selection and management of broodstock are discussed.

Fisheries profile mapping of coastal districts in Maharashtra state through GIS

In recent times, GIS is being increasingly used as a decision support system for management of fisheries and aquaculture. It provides new innovative approaches of the dynamic relations that characterize this sector. In this context, a study is conducted based on the secondary data of a major maritime state, Maharashtra, where mapping of fisheries profile of coastal districts in the state is performed through GIS tool having critical geographic dimensions. This paper aims to map information of the state which can be used for the purpose of planning and decision making as each aspect of map has a different component involved. For this purpose, at the core of the system, the data were accessed and integrated from different sources mainly from the five coastal districts of Maharashtra state. Data were brought in tabular form through Microsoft Excel and then joined to Map info Professional version 8.0 GIS software was used with the digitized map of Maharashtra state to enable mapping. This was further synchronized and integrated to generate four thematic maps searchable on several criteria. Map 1 contains the searchable criteria as regards to the fish growth for the year 1997-2004 and fish seed production for the year 2003-04. Map 2 contains fisher population along with their occupation for the year 1992. Map 3 contains brackish water and shrimp farming production and culture area. Map 4 contains infrastructural facilities which include type of boats etc. With this mapping, planners and various stakeholders have accessible information as regards to the various components of fisheries in the state of Maharashtra.