Growth and production performance of red tilapia and Nile tilapia (Oreochromis niloticus Lin.) under low-input culture system

Comparative production potential of red tilapia (a mutant hybrid of Oreochromis mossambicus) and Nile tilapia (Oreochromis niloticus) under low-input aquaculture was studied in six ponds of 360 m² each with an average water depth of 90 cm. Three ponds were stocked with fingerlings of O. niloticus (average weight 11.4±3.48 g) while three other ponds were stocked with red tilapia (average weight 10.72±2.5 g) at a density of 20,000 fingerlings/ha. Supplementary feed consisting of rice bran was given daily at 4-6% of standing biomass. Ponds were fertilized at fortnightly intervals with cattle manure 750 kg/ ha. After six months of rearing, gross fish productions of 3,218 and 3,017 kg/ha were obtained from O. niloticus and red tilapia ponds, respectively. Of this, table size fish (>80 g in size) production amounted to 2,366 and 2,823 kg/ha from O. niloticus and red tilapia culture, respectively. Analysis of cost and benefits showed higher benefit from red tilapia culture.

Food preference and intake pattern of Penaeus monodon (Fab.) in semi-intensive culture system

P. monodon juveniles prefer natural food than artificial feed in their initial stage of rearing. However, the percentage preference towards artificial feed increases as the days of culture/average body weight increases. Consumption of artificial feed was high during evening meal (1700h) and low during night meal (2200h). The index of intestine fullness was high (8.4±0.7) during initial stage of rearing, compared to that of final stage of rearing (4.9±0.4), probably due to low nutritional value of intake material and low water depth at initial stage. At all stage of rearing omnivorous feeding habit was established and no evidence of diet periodicity in feeding activity was observed.

Effect of submerged aquatic vegetation on growth and survival of Penaeus monodon (Fab.)

An experiment was conducted in brackish water ponds with transplanted aquatic weed (Najas sp.) to study the effect of submerged aquatic vegetation on culture of Penaeus monodon. Out of six ponds three were without any aquatic vegetation (T1) while in other three ponds weeds were planted covering 40% of the pond bottom (T2). Hatchery produced post larva of P. monodon (0.006 g) were stocked in all ponds at a density of 40,000/ha. Shrimps were fed twice daily with commercial and formulated feed prepared from locally available ingredients. After 105 days of rearing shrimp of highest average weight (63.18g) was obtained from T2 with a survival rate of 25.90% and the total production was obtained at 654.54 kg/ha. The average weight, survival and total production of shrimp in T1 were 35.0 g, 28.76% and 405.63 kg/ha, respectively.

Effects of animal and plant protein diets on growth of Indian major carp (Labeo rohita Ham.) fingerlings

An experiment was conducted with Labeo rohita fingerlings in an indoor static fish rearing water system of glass made aquaria. Five experimental diets A, B, C, D and E were formulated containing 33% dietary protein level in five treatments each having two replicates containing 12 fingerlings of mean total initial weight of 13.00±0.2g. Sixty days of feeding trial in this experiment showed that fish fed on diet 'A' containing fish meal and diet 'E' containing mixed plant sources protein had significantly highest and lowest growth respectively. However, no significant difference of growth was found in fish fed on diets C and D containing meat and bone meal, and mix of animal protein source diets respectively. The result showed that the apparent protein digestibility (APD) of diets 'A' and 'E' had significantly best and least values respectively. Food conversion ratio (FCR) and protein efficiency ratio (PER) ranged between 1.37 to 2.17 and 1.38 to 2.18 respectively. On the basis of observed FCR and PER diets 'A' and 'E' produced significantly highest and lowest growth respectively.

Rearing of Labeo bata in sewage-fed fish culture pond

Labeo bata is a highly priced fish which commanding good consumer preference, even if the size is very small (15 to 20 g each), especially in West Bengal. With a view to evaluating the culture potential of this fish, repeated experiments were conducted at the Wastewater Aquaculture Division of the Central Institute of Freshwater Aquaculture, Rahara, West Bengal, during 1991-93 in ponds fed with treated domestic sewage effluent. An average production of 1270.63 kg per ha was obtained within 6 to 10 months for a uniform stocking density of 50,000 seed per ha. The average size attained by the fish in the different sets of experiments varied from 23.3 to 37.9 g as against the initial weight of 0.096 to 0.193 g. Details of management, growth, survival, quantity of sewage used and pond nutrient status and productivity are dealt with in this communication.

Field application of anabolic steroids in carp seed production. II. Rearing of spawn to fry stage.

Two synthetic androgenic steroids, Ethylestrenol (17 β - Hydroxy - 17 α ethyl - estr - 4 - en - 3 - one) and Stanozolal (17 β - Hydroxy- 17 α - methyl - 5 a - androstano - 3, 2 - C - pyrazole) were fed via diet at 3 ppm to the spawn of Rohu and Mrigal which were reared up to fry stage over a period of 15 days in earthen carp nurseries. Both hormones enhanced growth of spawn. A maximum of 25.78% increase in length and 25.69% increase in weight as compared to the controls has been recorded. Growth rate was recorded to be 0.8 mm & 2.48 mg/day (control), and 1.13 mm & 2.67 mg/day (Stanozolol treated) in case of Mrigal spawn; and 0.91 mm & 2.39 mg/day (control), 1.12 mm & 2.90 mg/day (Ethylestrenol treated), and 1.10 mm & 2.57 mg/day (Stanozolol treated) in case of Rohu spawn. A decrease in the values of Relative Condition Factor upon hormone administration was also noticed.

Evaluation of some formulated diets for rearing the post-larvae of Macrobrachium rosenbergii

Macrobrachium rosenbergii post-larvae with an average weight of 6.26 ± 0.23 mg and an average length of 10.67 ± 0.13 mm were fed with one of the five experimental diets having graded levels of lipid (5.0, 7.5, 10.0, 12.5 and 15.0%) to satiation two times a day to study their effect on growth, survival and feed utilisation. The highest weight gain was observed in post-larvae fed 7.5% lipid, although there was no significant difference (p>0.05) between groups fed 7.5 and 10.0% lipid. Similar effect was observed in the specific growth rate and protein efficiency ratio of post-larvae fed the diet with 7.5 and 10.0% lipid. The lowest feed conversion ratio was obtained in postlarvae fed the diet with 7.5% lipid. The survival rates of post-larvae fed on various levels of dietary lipid differed significantly (p<0.05) after 15 days of rearing period. Significantly lower survival was observed in the diet with 15.0% lipid level. Dietary lipid did not significantly affect prawn carcass protein, lipid and ash contents. Based on the data analysis (ANOVA – one way analysis), the dietary lipid requirement for 15 days of nursery rearing of Macrobrachium rosenbergii post-larvae was estimated to be 7.5 to 10.0% under experimental conditions in this study.

Effect of combined thyroxine and cortisol treatment on hatching of eggs, post-embryonic growth and survival of larvae of Heteropneustes fossilis

In order to record the effects of thyroxine and cortisol (individual/combined) on hatching, post-embryonic growth and survival of larvae of Heteropneustes fossilis, newly fertilized eggs were given bath immersion treatments of L-thyroxine (T sub(4); 0.05 mg/l), cortisol (0.50 mg/l) and T sub(4)+ cortisol (0.05 mg/l+0.50 mg/l) for 15 days. Hatching of eggs, growth and survival of the larvae improved significantly (P<0.001) in the hormone treated groups as compared to those of control. The frequency of deformities was reduced in the combined hormone treatment group. The present observations suggest that the advanced digestive function probably induced by T sub(4)+cortisol treatment might have resulted in improvement in food utilization during the critical phases of first feeding and promoted vital developmental processes resulting in uniform growth, decreased mortality, better survival and transformation of larvae to juveniles. This combined hormone therapy appears to have practical utility in fish hatchery practice for better success in larval rearing.

Larval rearing of a freshwater prawn Macrobrachium gangeticum

Observations were made on the larval development of a freshwater prawn, Macrobrachium gangeticum, which revealed that hatching occurred in freshwater but the larvae failed to survive after 2nd molting. Salinity was necessary for survival of the larvae after 2nd molting. The complete larval development involved nine larval stages and the 10th stage was considered to be the post-larva which measured between 4.5 and 5.0 mm in length. All the larval stages were completed within 26 days of hatching. Specimens from each larval stage were taken out and examined under a microscope.

Effect of high and low cost brood feeds on gonado-somatic index and fecundity of freshwater prawn Macrobrachium rogenbergii de Man

Studies were conducted to observe the effects of different types of feeds on the gonado-somatic index (GSI) and fecundity of freshwater prawn Macrobrachium rogenbergii. Three different treatments (T1 T2 and T3) were designed with three types of feed as follows: (i) Saudi-Bangla Prawn feed 100% - T1 (ii) SaudiBangla prawn feed 50%+ local feed 50%- T2 and (iii) local feed 100%- T3. The results showed that the average value of gonado-somatic index (GSI) was 14.39, 14.35 and 14.36 and the average fecundity of M. rogenbergiiwas 99,741, 98,125 and 97,911 in T1 T2 and T3 respectively. No significant difference (p>00.5) was between gonado-somatic indices (GSI) and fecundities of M. rogenbergii among different feeding trails. The price of Saudi-Bangla prawn feed was very high (Tk. 23/kg) than the local feed (Tk. 14/kg). So, use of local feed was recommended for M. rogenbergii brood rearing.

Use of crude salt in rearing of freshwater giant prawn, Macrobrachium rosenbergii (de Man) larvae

Three different types of culture media: (i) 100% brine (B 100 ), (ii) 75% brine and 25% crude salt (B 75 CS 25 ), and 50% brine and 50% crude salt (B 50 CS 50) were tested to evaluate the possible use of brackish water reconstituted from the crude salt for the production of M. rosenbergii post-larvae. The production rate of 25.26±0.20 PI/l with a corresponding survival rate of 84.20±0.66% was significantly higher (P<0.05) for the larvae reared on B100 than that of 22.10±0.57 Pl/l with a corresponding survival rate of 73.68±1.89% on B50CS50. Larvae cultured on B75CS25 did not show any significant difference (P<0.05) in production as well as in survival of post-larvae than that on B100. The result shows that, for rearing of prawn larvae, use of brine can be replaced up to 25% without any undue reduction in production of post-larvae. However, the production as well as survival rate of post-larvae with 50% replacement (B50CS50) is also appreciable. It is assumed that the mineral constituents of natural seawater might have some triggering effects on prawn larvae in closing their larval cycle.

Effect of pond aeration on growth and survival of Penaeus monodon Fab.

The effect of paddle wheel aeration on shrimp growth and survival were studied at a commercial farm at Chandipur coast of Orissa, India, at different stocking densities of Penaeus monodon. Four different aeration patterns were adopted and evaluated. Influence of individual aeration pattern on average survival rate was not highly significant (p<0.05) at different stocking densities, while different aeration patterns had significant influence (prearing, while it was reduced to significant level towards the last phase of rearing as number of aerator and hour of operation increased.

Development of artificial breeding techniques for long-whiskered catfish, Sperata aor and giant river catfish, Sperata seenghala of Bangladesh

Sperata aor and S. seenghala are the two important native catfishes of Bangladesh but commercial farming of these species is not possible due to lack of naturally collected or artificially produced seeds for stocking. Attempts were made to develop techniques for seed production by artificial breeding and nursery-rearing of fries of these catfishes. A total of 60 S. seenghala (750-1,500 g) and 10 S. aor (600-1,000 g) broods were collected from the Brahmaputra river-basin and floodplains in Mymensingh region four months prior to their breeding season. The collected brood fishes were reared in separate earthen ponds with supplementary feeds comprising of rice bran (40%), mustard oil cake (29%), fish meal (30%) and vitamin-premix (1 %). Three experiments were conducted to optimize the hormone dose. A total of nine S. seenghala females weighing from 750 to 1,500 g were given an initial and resolving dose of 12-20 and 16-24 mg PG/kg body weight, respectively. The males weighing from 650-950 g were administered a single dose of 18-26 mg PG/kg body weight at the time of the time of administering the resolving dose to the females. The females ovulated partially and the eggs were examined under a compound microscope, but most of them were found to be less ripe or damaged. Collection of milt by stripping the males was not successful. The testes were taken out and sperm were observed to be non-motile and less developed. In view of stimulating natural propagation of S. seenghala, artificial holes (nests) were constructed in the pond bottom. Each hole was 0.7 m in diameter and 0.3 m in depth. A total of 10 holes were made and then 10 pairs of S. seenghala breeders (800-1,200 g) were stocked in the pond. In mid February, 3,000 fry of S. seenghala with a mean length of 4.60 cm and weight of 0.36 g were collected by repeated netting followed by drying of the pond. The fry were then stocked in a nursery pond and fed with commercial feed (SABINCO starter-1). The average length and weight of the fingerlings were 9.01 cm and 3.95 g, respectively and the estimated survival was 60% after two months of rearing. S. aor did not respond to natural spawning. Further study is essential to develop techniques for their successful artificial and natural breeding.

Over-wintering performance of mixed sex and monosex tilapia (Oreochromis niloticus L.) in Bangladesh

An experiment was conducted for six months in 6 experimental ponds (each size 80 of m2) to assess the over-wintering performance between mixed sex and monosex tilapia, Oreochromis niloticus. The experiment was carried out with two treatments each with three replicates. In the first treatment (T1), mixed sex tilapia were stocked in 3 ponds with a mean initial of 4.80±0.18 g. In the second treatment (T2), monosex tilapia were stocked in another 3 ponds with a mean initial weight of 4.81 ±0.20 g. Each pond was stocked with 250 fingerlings. Fish were fed at the rate of 6% of fish body weight at the beginning. The feeding rate was gradually reduced to 2% for the third month and finally increased to 3% for rest of the period. Water quality was monitored fortnightly and the ranges were: temperature17.86-29.10°C, dissolved oxygen 4.25-6.10 mg/1, pH 6.97-7.20 and transparency 24.10-36.50 cm. After 6 months of rearing monosex tilapia attained a significantly (pand survival (%)values between the mixed sex and monosex tilapia. The production of monosex tilapia (3723.10 kg/ha) was about 32% higher than that of mixed sex tilapia (2776.28 kg/ha). The net profit/ha generated from the 6 months culture period was calculated as Tk. 43,311.45 and. 69,277.32/- for mixed sex and monosex tilapia respectively. The results of the present study suggested that it is possible to successfully culture tilapia during the winter period and the culture of monosex tilapia is more profitable due to its higher growth rate than that of mixesex tilapia.

Status of fish broodstock management and seed production in Bangladesh

The study was conducted with the broad objectives to assess the existing situation of broodstock management and fish seed production in private fish seed farms in Bangladesh. The data were collected from 100 private hatcheries and 40 nurseries in seven upazilas under four districts. There was no shed in forty hatcheries and the owners faced many problems. Brood fish ponds were found suitable for rearing brood fish. About 66% of the hatchery owners collected brood fish from their own ponds and ponds of neighboring areas. Activities like pond preparation; manuring and supplementary feeding were done properly but stocking density of brood fish in 76% of the hatcheries was 3,000-7,000 kg/ha. Infection of argulosis was found in brood fish of 87% of the hatcheries. About 67% of the hatchery owners practiced inter-species crossing. Major problems faced by the hatchery owners were argulosis of brood fish, unavailability of pure brood stock, inadequate brood fish pond. The hatchery owners were found interested to find out the preventive measures of argulosis, develop pure brood stock of indigenous carp and import pure strain of exotic carp. According to the nursery operators, they cultivated hybrid fry because of high demand, rapid growth and good taste. Problems of using hatchery spawn as mentioned by the nursery operators were inbreeding, under sized and aged brood stock, stunted growth, physical deformities and high mortality of spawn due to unknown causes.