Determination of population structure and stock composition of chum salmon (Oncorhynchus keta) in Russia determined with microsatellites

Variation at 14 microsatellite loci was examined in 34 chum salmon (Oncorhynchus keta) populations from Russia and evaluated for its use in the determination of population structure and stock composition in simulated mixed-stock fishery samples. The genetic differentiation index (Fst) over all populations and loci was 0.017, and individual locus values ranged from 0.003 to 0.054. Regional population structure was observed, and populations from Primorye, Sakhalin Island, and northeast Russia were the most distinct. Microsatellite variation provided evidence of a more fine-scale population structure than those that had previously been demonstrated with other genetic-based markers. Analysis of simulated mixed-stock samples indicated that accurate and precise regional estimates of stock composition were produced when the microsatellites were used to estimate stock compositions. Microsatellites can be used to determine stock composition in geographically separate Russian coastal chum salmon fisheries and provide a greater resolution of stock composition and population structure than that previously provided with other techniques.

Age composition and distribution of red drum (Sciaenops ocellatus) in offshore waters of the north central Gulf of Mexico: an evaluation of a stock under a federal harvest moratorium

Because of a lack of fishery-dependent data, assessment of the recovery of fish stocks that undergo the most aggressive form of management, namely harvest moratoriums, remains a challenge. Large schools of red drum (Sciaenops ocellatus) were common along the northern Gulf of Mexico until the late 1980s when increased fishing effort quickly depleted the stock. After 24 years of harvest moratorium on red drum in federal waters, the stock is in need of reassessment; however, fisherydependent data are not available in federal waters and fishery-independent data are limited. We document the distribution, age composition, growth, and condition of red drum in coastal waters of the north central Gulf of Mexico, using data collected from a nearshore, randomized, bottom longline survey. Age composition of the fishery-independent catch indicates low mortality of fish age 6 and above and confirms the effectiveness of the federal fishing moratorium. Bottom longline surveys may be a cost-effective method for developing fishery-independent indices for red drum provided additional effort can be added to nearshore waters (<20 m depth). As with most stocks under harvest bans, effective monitoring of the recovery of red drum will require the development of fishery-independent indices. With limited economic incentive to evaluate non-exploited stocks, the most cost-effective approach to developing such monitoring is expansion of existing fishery independent surveys. We examine this possibility for red drum in the Gulf of Mexico and recommend the bottom longline survey conducted by the National Marine Fisheries Service expand effort in nearshore areas to allow for the development of long-term abundance indices for red drum.

Estimating species and size composition of rockfishes to verify targets in acoustic surveys of untrawlable areas

Rockfish (Sebastes spp.) biomass is difficult to assess with standard bottom trawl or acoustic surveys because of their propensity to aggregate near the seafloor in highrelief areas that are inaccessible to sampling by trawling. We compared the ability of a remotely operated vehicle (ROV), a modified bottom trawl, and a stereo drop camera system (SDC) to identify rockfish species and estimate their size composition. The ability to discriminate species was highest for the bottom trawl and lowest for the SDC. Mean lengths and size distributions varied among the gear types, although a larger number of length measurements could be collected with the bottom trawl and SDC than with the ROV. Dusky (S. variabilis), harlequin (S. variegatus), and northern rockfish (S. polyspinis), and Pacific ocean perch (S. alutus) were the species observed in greatest abundance. Only dusky and northern rockfish regularly occurred in trawlable areas, whereas these two species and many more occurred in untrawlable areas. The SDC was able to resolve the height of fish off the seafloor, and some of the rockfish species were observed only near the seafloor in the acoustic dead zone. This finding is important, in that fish found exclusively in the acoustic dead zone cannot be assessed acoustically. For these species, methods such as bottom trawls, long-lines, or optical surveys using line transect or area swept methods will be the only adequate means to estimate the abundance of these fishes. Our results suggest that the selection of appropriate methods for verifying targets will depend on the habitat types and species complexes to be examined.

Parameterizing probabilities for estimating age-composition distributions for mixture models

When estimating parameters that constitute a discrete probability distribution {pj}, it is difficult to determine how constraints should be made to guarantee that the estimated parameters { pˆj} constitute a probability distribution (i.e., pˆj>0, Σ pˆj =1). For age distributions estimated from mixtures of length-at-age distributions, the EM (expectationmaximization) algorithm (Hasselblad, 1966; Hoenig and Heisey, 1987; Kimura and Chikuni, 1987), restricted least squares (Clark, 1981), and weak quasisolutions (Troynikov, 2004) have all been used. Each of these methods appears to guarantee that the estimated distribution will be a true probability distribution with all categories greater than or equal to zero and with individual probabilities that sum to one. In addition, all these methods appear to provide a theoretical basis for solutions that will be either maximum-likelihood estimates or at least convergent to a probability distribut

Diet composition and food habits of demersal and pelagic marine fishes from Terengganu waters, east coast of Peninsular Malaysia

Fish stomachs from 18 demersal and pelagic fishes from the coast of Terengganu in Malaysia were examined. The components of the fishes’ diets varied in number, weight, and their frequency of occurrence. The major food items in the stomachs of each species were determined using an Index of Relative Importance. A conceptual food web structure indicates that fish species in the study area can be classified into three predatory groups: (1) predators on largely planktivorous or pelagic species; (2) predators on largely benthophagous or demersal species; and (3) mixed feeders that consume both pelagic and demersal species.

Diet composition of fish species from the southern continental shelf of Colombia

The diet composition of 30 fish species belonging to 16 families from the Pacific Coast of Colombia is described. Benthic crustaceans (37.5%) and bony fishes (23.7%, chiefly demersal) were the most important food items for the fish species analyzed. Data on diet composition of the fish species are presented for the first time which can be a source of information for trophic modeling.

The evolution of body muscle composition of the African catfish (Clarias gariepinus) (Burchell 1822)

Changes in body muscle composition of Clarias gariepinus were studied in fish reared from 1.08 g to 383 g mean body weight in a 201-day culture period. Changes in the amount of protein content, dry matter and ash free dry matter in the muscle tissue can be described as a function of body weight. The percentage of protein content was observed to be higher in bigger fish. Fat content was low throughout the fingerling stage. Specific growth rate decreased significantly at 400 g mean body weight (P<0.05) while feed conversion rate increased. The conclusion, based on the culture conditions in this study, is that the optimal weight for harvesting C. gariepinus is 400 g.

Diet composition and daily ration estimates of selected trawl-caught fishes in San Miguel Bay, Philippines

The diet composition of fish caught in San Miguel Bay, Philippines, in April and May 1993 was studied. The diets of tiger-tooth croaker (Otolithes ruber), commerson's anchovy (Stolephorus commersonii); and the Indian anchovy (Stolephorus indicus) consisted mainly of zooplankton, primarily crustaceans. The stomach content of orangefin ponyfish (Leiognathus bindus) was found to consist mostly of detritus and unidentified materials. Daily rations estimated were: 1.90 g day super(1) for O. ruber of 17.3 g mean body weight (BW), 0.078 g day super(1) for S. commersonii) of 3.8 g mean BW, 0.062 g day super(1) for S. indicus of 3.9 g mean BW and 0.56 g day super(1) for L. bindus of 7.7 g mean BW.

Improving Shepherd's Length Composition Analysis (SLCA) method for growth parameter estimations

Shepherd's "weekly parametric" method for estimating the parameter L sub( infinity ) and K of the von Bertalanffy growth function from length-frequency data often fails to converge, and usually overestimates K. It is shown that this is due to overcounting of the frequencies associated with large, slow growing fish, and that both of these problems can be completely overcome by a simple change in the way the scoring function is formulated.

Soft Flesh in Sablefish, Anoplopoma fimbria, of Southeastern Alaska: Relationships with Depth, Season, and Biochemistry

The condition of soft-textured flesh in commercially harvested sablefish, Anoplopoma fimbria, from southeastern Alaska was investigated by National Marine Fisheries Service (NMFS) scientists from the Alaska Fisheries Science Center’s Auke Bay Laboratories (ABL) in Alaska and the Northwest Fisheries Science Center in Seattle, Wash. Sablefish were sampled by longline, pot, and trawl at five sites around Chichagof Island at depths of 259–988 m in the summer of 1985 and at depths of 259–913 m in the winter of 1986. At the time of capture and data collection, sablefish were categorized as being “firm” or “soft” by visual and tactile examination, individually weighed, measured for length, and sexed. Subsamples of the fish were analyzed and linear regressions and analyses of variance were performed on both the summer (n = 242) and winter (n = 439) data for combinations of chemical and physical analyses, depth of capture, weight vs. length, flesh condition, gonad condition, and sex. We successfully identified and selected sablefish with firm- and soft-textured flesh by tactile and visual methods. Abundance of firm fish in catches varied by season: 67% in winter and 40% in summer. Winter catches may give a higher yield than summer catches. Abundance of firm fish catches also varied with depth. Firm fish were routinely found shallower than soft fish. The highest percentage of firm fish were found at depths less than 365 m in summer and at 365–730 m in winter, whereas soft fish were usually more abundant at depths greater than 731 m. Catches of firm fish declined with increasing depth. More than 80% of the fish caught during winter at depths between 365 and 730 m had firm flesh, but this declined to 48% at these depths in summer. Longlines and pots caught similar proportions of firm and soft fish with both gears catching more firm than soft fish. Trawls caught a higher proportion of soft fish compared to longlines and pots in winter. Chemical composition of “firm” and “soft” fish differed. On average “soft” fish had 14% less protein, 12% more lipid, and 3% less ash than firm fish. Cooked yields from sablefish with soft-textured flesh were 31% less than cooked yields from firm fish. Sablefish flesh quality (firmness) related significantly to the biochemistry of white muscle with respect to 11 variables. Summer fish of all flesh conditions averaged 6% heavier than winter fish. Regulating depth of fishing could increase the yield from catches, but the feasibility and benefits from this action will require further evaluation and study. Results of this study provide a basis for reducing the harvest of sablefish with soft flesh and may stimulate further research into the cause and effect relationship of the sablefish soft-flesh phenomenon.

Size-composition of Annual Landings in the White Shrimp, Litopenaeus setiferus, Fishery of the Northern Gulf of Mexico, 1960–2006: Its Trend and Relationships with Other Fishery-dependent Variable

The potential for growth overfishing in the white shrimp, Litopenaeus setiferus, fishery of the northern Gulf of Mexico appears to have been of limited concern to Federal or state shrimp management entities, following the cataclysmic drop in white shrimp abundance in the 1940’s. As expected from surplus production theory, a decrease in size of shrimp in the annual landings accompanies increasing fishing effort, and can eventually reduce the value of the landings. Growth overfishing can exacerbate such decline in value of the annual landings. We characterize trends in size-composition of annual landings and other annual fishery-dependent variables in this fishery to determine relationships between selected pairs of these variables and to determine whether growth overfishing occurred during 1960–2006. Signs of growth overfishing were equivocal. For example, as nominal fishing effort increased, the initially upward, decelerating trend in annual yield approached a local maximum in the 1980’s. However, an accelerating upward trend in yield followed as effort continued to increase. Yield then reached its highest point in the time series in 2006, as nominal fishing effort declined due to exogenous factors outside the control of shrimp fishery managers. The quadratic relationship between annual yield and nominal fishing effort exhibited a local maximum of 5.24(107) pounds (≈ MSY) at a nominal fishing effort level of 1.38(105) days fished. However, annual yield showed a continuous increase with decrease in size of shrimp in the landings. Annual inflation-adjusted ex-vessel value of the landings peaked in 1989, preceded by a peak in annual inflation-adjusted ex-vessel value per pound (i.e. price) in 1983. Changes in size composition of shrimp landings and their economic effects should be included among guidelines for future management of this white shrimp

Long-term Trends in Catch Composition from Elasmobranch Derbies in Elkhorn Slough, California

Long-term trends in the elasmobranch assemblage of Elkhorn Slough, Monterey Bay, California, were analyzed by documenting species composition and catch per unit effort (CPUE) from 55 sport fishing derbies that occurred during May, June, and July, from 1951 until 1995. The most abundant species (bat ray, Myliobatis californica; shovelnose guitarfish, Rhinobatos productus; and leopard shark, Triakis semifasciata) were also analyzed for size-weight relationships, trends in size class distribution, stage of maturity, and sex ratios. Changes in species composition over the course of the derbies included the near complete disappearance of shovelnose guitarfish by the 1970’s and a slight increase in the abundance of minor species (mainly smoothhounds, Mustelus spp., and thornback, Platyrhinoidis triseriata) starting in the mid 1960’s. The relative abundance of bat rays in the catch steadily increased over the years while the relative abundance of leopard sharks declined during the last two decades. However the average number of bat rays and leopard sharks caught per derby declined during the last two decades. Fishing effort appeared to increase over the course of the derbies. There were no dramatic shifts in the size class distribution data for bat rays, leopard sharks, or shovelnose guitarfish. The catch of bat rays and leopard sharks was consistently dominated by immature individuals, while the catch of shovelnose guitarfish was heavily dominated by adults. There was evidence of sexual segregation in either immature or mature fish in all the species. Female bat rays and shovelnose guitarfish were larger than their male counterparts and outnumbered males nearly 2:1. Female and male leopard sharks were more nearly equal in size and sex ratio. Changes in species composition are likely due to fishing pressure, shifts in the prevailing oceanographic conditions, and habitat alteration in Elkhorn Slough. The sex ratios, stage of maturity, and size class distributions provide further evidence for the theory that Elkhorn Slough functions as a nursery habitat for bat rays and leopard sha

Age and length composition of Columbia Basin chinook, sockeye, and coho salmon at Bonneville Dam in 2001

In 2001, representative samples of adult Columbia Basin chinook (Oncorhynchus tshawytscha), sockeye (O. nerka), and coho salmon (O. kisutch) populations at Bonneville Dam were collected. Fish were trapped, anesthetized, sampled for scales and biological data, revived, and then released adult migrating salmonids. Scales were examined to estimate age composition; the results contributed to an ongoing database for age class structure of Columbia Basin salmon populations. Based on scale analysis of chinook salmon, four-year-old fish (from brood year [BY] 1997) comprised 88% of the spring chinook, 67% of the summer chinook, and 42% of the Bright fall chinook salmon population. Five-year-old fish (BY 1996) comprised 9% of the spring chinook, 14% of the summer chinook, and 9% of the fall chinook salmon population. The sockeye salmon population at Bonneville was predominantly four-year-old fish (81%), with 18% returning as five-year-olds in 2001. The coho salmon population was 96% three-year-old fish (Age 1.1). Length analysis of the 2001 returns indicated that chinook salmon with a stream-type life history are larger (mean length) than the chinook salmon with an ocean-type life history. Trends in mean length over the sampling period for returning 2001 chinook salmon were analyzed. Chinook salmon of age classes 0.2 and 1.3 show a significant increase in mean length over time. Age classes 0.1, 0.3, 0.4, 1.1, 1.2, and 1.4 show no significant change over time. A year class regression over the past 12 years of data was used to predict spring, summer, and Bright fall chinook salmon population sizes for 2002. Based on three-year-old returns, the relationship predicts four-year-old returns of 132,600 (± 46,300, 90% predictive interval [PI]) spring chinook and 44,200 (± 11,700, 90% PI) summer chinook salmon for the 2002 runs. Based on four-year-old returns, the relationship predicts five-year-old returns of 87,800 (± 54,500, 90% PI) spring, 33,500 (± 11,500, 90% PI) summer, and 77,100 (± 25,800, 90% PI) Bright fall chinook salmon for the 2002 runs. The 2002 run size predictions should be used with caution; some of these predictions are well beyond the range of previously observed data.

Age and length composition of Columbia Basin chinook, sockeye, and coho salmon at Bonneville Dam in 2000

In 2000, representative samples of adult Columbia Basin chinook (Oncorhynchus tshawytscha), sockeye (O. nerka), and coho salmon (O. kisutch), populations were collected at Bonneville Dam. Fish were trapped, anesthetized, sampled for scales and biological data, allowed to revive, and then released. Scales were examined to estimate age composition and the results contribute to an ongoing database for age class structure of Columbia Basin salmon populations. Based on scale analysis, four-year-old fish (from brood year (BY) 1996) were estimated to comprise 83% of the spring chinook, 31% of the summer chinook, and 32% of the upriver bright fall chinook salmon population. Five-year-old fish (BY 1995) were estimated to comprise 2% of the spring chinook, 26% of the summer chinook, and 40% of the fall chinook salmon population. Three-year-old fish (BY 1997) were estimated to comprise 14% of the spring chinook, 42% of the summer chinook, and 17% of the fall chinook salmon population. Two-year-olds accounted for approximately 11% of the fall chinook population. The sockeye salmon population sampled at Bonneville was predominantly four-year-old fish (95%), and the coho salmon population was 99.9% three-year-old fish (Age 1.1). Length analysis of the 2000 returns indicated that chinook salmon with a stream-type life history are larger (mean length) than the chinook salmon with an ocean-type life history. Trends in mean length over the sampling period were also analysis for returning 2000 chinook salmon. Fish of age classes 0.2, 1.1, 1.2, and 1.3 have a significant increase in mean length over time. Age classes 0.3 and 0.4 have no significant change over time and age 0.1 chinook salmon had a significant decrease in mean length over time. A year class regression over the past 11 years of data was used to predict spring and summer chinook salmon population sizes for 2001. Based on three-year-old returns, the relationship predicts four-year-old returns of 325,000 (± 111,600, 90% Predictive Interval [PI]) spring chinook and 27,800 (± 29,750, 90% PI) summer chinook salmon. Based on four-year-old returns, the relationship predicts five-year-old returns of 54,300 (± 40,600, 90% PI) spring chinook and 11,000 (± 3,250, 90% PI) summer chinook salmon. The 2001 run size predictions used in this report should be used with caution, these predictions are well beyond the range of previously observed data.