Guide to the identification of larval and early juvenile pricklebacks (Perciformes: Zoarcoidei: Stichaeidae) in the northeastern Pacific Ocean and Bering Sea

Stichaeidae, commonly referred to as pricklebacks, are intertidal and subtidal fishes primarily of the North Pacific Ocean. Broad distribution in relatively inaccessible and undersampled habitats has contributed to a general lack of information about this family. In this study, descriptions of early life history stages are presented for 25 species representing 18 genera of stichaeid fishes from the northeastern Pacific Ocean, Bering Sea, and Arctic Ocean Basin. Six of these species also occur in the North Atlantic Ocean. Larval stages of 16 species are described for the first time. Additional information or illustrations intended to augment previous descriptions are provided for nine species. For most taxa, we present adult and larval distributions, descriptions of morphometric, meristic, and pigmentation characters, and species comparisons, and we provide illustrations for preflexion through postflexion or transformation stages. New counts of meristic features are reported for several species.

Reproductive dynamics of female spotted seatrout (Cynoscion nebulosus) in South Carolina

We describe reproductive dynamics of female spotted seatrout (Cynoscion nebulosus) in South Carolina (SC). Batch fecundity (BF), spawning frequency (SF), relative fecundity (RF), and annual fecundity (AF) for age classes 1−3 were estimated during the spawning seasons of 1998, 1999, and 2000. Based on histological evidence, spawning of spotted seatrout in SC was determined to take place from late April through early September. Size at first maturity was 248 mm total length (TL); 50% and 100% maturity occurred at 268 mm and 301 mm TL, respectively. Batch fecundity estimates from counts of oocytes in final maturation varied significantly among year classes. One-year-old spotted seatrout spawned an average of 145,452 oocytes per batch, whereas fish aged 2 and 3 had a mean BF of 291,123 and 529,976 oocytes, respectively. We determined monthly SF from the inverse of the proportion of ovaries with postovulatory follicles (POF) less than 24 hours old among mature and developing females. Overall, spotted seatrout spawned every 4.4 days, an average of 28 times during the season. A chronology of POF atresia for water temperature >25°C is presented. Length, weight (ovary-free), and age explained 67%, 65%, and 58% of the variability in BF, respectively. Neither RF (number of oocytes/g ovary-free weight) nor oocyte diameter varied significantly with age. However, RF was significantly greater and oocyte diameter was smaller at the end of the spawning season. Annual fecundity estimates were approximately 3.2, 9.5, and 17.6 million oocytes for each age class, respectively. Spotted seatrout ages 1−3 contributed an average of 29%, 39%, and 21% to the overall reproductive effort according to the relative abundance of each age class. Ages 4 and 5 contributed 7% and 4%, respectively, according to predicted AF values.

Factors influencing the timing and frequency of spawning and fecundity of the goldlined seabream (Rhabdosargus sarba) (Sparidae) in the lower reaches of an estuary

We have studied the reproductive biology of the goldlined seabream (Rhabdosargus sarba) in the lower Swan River Estuary in Western Australia, focusing particularly on elucidating the factors influencing the duration, timing, and frequency of spawning and on determining potential annual fecundity. Our results demonstrate that 1) Rhabdosargus sarba has indeterminate fecundity, 2) oocyte hydration commences soon after dusk (ca. 18:30 h) and is complete by ca. 01:30−04:30 h and 3) fish with ovaries containing migratory nucleus oocytes, hydrated oocytes, or postovulatory follicles were caught between July and November. However, in July and August, their prevalence was low, whereas that of fish with ovaries containing substantial numbers of atretic yolk granule oocytes was high. Thus, spawning activity did not start to peak until September (early spring), when salinities were rising markedly from their winter minima. The prevalence of spawning was positively correlated with tidal height and was greatest on days when the tide changed from flood to ebb at ca. 06:00 h, i.e., just after spawning had ceased. Because our estimate of the average daily prevalence of spawning by females during the spawning season (July to November) was 36.5%, individual females were estimated to spawn, on average, at intervals of about 2.7 days and thus about 45 times during that period. Therefore, because female R. sarba with total lengths of 180, 220, and 260 mm were estimated to have batch fecundities of about 4500, 7700, and 12,400 eggs, respectively, they had potential annual fecundities of about 204,300, 346,100 and 557,500 eggs, respectively. Because spawning occurs just prior to strong ebb tides, the eggs of R. sarba are likely to be transported out of the estuary into coastal waters where salinities remain at ca. 35‰. Such downstream transport would account for the fact that, although R. sarba exhibits substantial spawning activity in the lower Swan River Estuary, few of its early juveniles are recruited into the nearshore shallow waters of this estuary.

The early life history of swordfish (Xiphias gladius) in the western North Atlantic

Lengths and ages of sword-fish (Xiphias gladius) estimated from increments on otoliths of larvae collected in the Caribbean Sea, Florida Straits, and off the southeastern United States, indicated two growth phases. Larvae complete yolk and oil globule absorption 5 to 6 days after hatching (DAH). Larvae <13 mm preserved standard length (PSL) grow slowly (~0.3 mm/d); larvae from 13 to 115 mm PSL grow rapidly (~6 mm/d). The acceleration in growth rate at 13 days follows an abrupt (within 3 days) change in diet, and in jaw and alimentary canal structure. The diet of swordfish larvae is limited. Larvae <8 mm PSL from the Caribbean, Gulf of Mexico, and off the southeastern United States eat exclusively copepods, primarily of one genus, Corycaeus. Larvae 9 to 11 mm eat copepods and chaetognaths; larvae >11 mm eat exclusively neustonic fish larvae. This diet indicates that young larvae <11 mm occupy the near-surface pelagia, whereas, older and longer larvae are neustonic. Spawning dates for larvae collected in various regions of the western North Atlantic, along with the abundance and spatial distribution of the youngest larvae, indicate that spawning peaks in three seasons and in five regions. Swordfish spawn in the Caribbean Sea, or possibly to the east, in winter, and in the western Gulf of Mexico in spring. Elsewhere swordfish spawn year-round, but spawning peaks in the spring in the north-central Gulf of Mexico, in the summer off southern Florida, and in the spring and early summer off the southeastern United States. The western Gulf Stream frontal zone is the focus of spawning off the southeastern coast of the United States, whereas spawning in the Gulf of Mexico seems to be focused in the vicinity of the Gulf Loop Current. Larvae may use the Gulf of Mexico and the outer continental shelf off the east coast of the United States as nursery areas. Some larvae may be transported northward, but trans-Atlantic transport of larvae is unlikely.

Age, growth, and reproduction of permit (Trachinotus falcatus) in Florida waters

We examined 536 permit (Trachinotus falcatus, 65–916 mm FL) collected from the waters of Florida Keys and from the Tampa Bay area on Florida’s Gulf coast to describe their growth and reproduction.Among permit that we sexed, females ranged from 266 to 916 mm in length (mean=617) and males ranged from 274 to 855 mm (mean=601). Ages of 297 permit ranging from 102 to 900 mm FL were estimated from thin-sectioned otoliths (sagittae). The large proportion of otoliths with an annulus on the margin and an otolith from an OTC-injected fish suggested that a single annulus was formed each year during late spring or early summer.Permit reach a maximum age of at least 23 years.Permit grew rapidly until an age of about five years, and then growth slowed considerably. Male and female von Bertalanffy growth models were not significantly different, and the sexes-combined growth model was FL=753.1(1–e –0.348(Age+0.585)). Gonad development was seasonal, and spawning occurred during late spring and summer over artificial and natural reefs at depths of 10–30 m. Ovaries that contained oocytes in the final stages of oocyte maturation or postovulatory follicles were found during May–July. We estimated that 50% of the females in the population had reached sexual maturity by 547 mm and an age of 3.1 years and that 50% of the males in the population had reached sexual maturity by 486 mm and an age of 2.3 years. Because Florida regulations restrict the maximum size of permit caught in recreational and commercial fisheries to 20-inch (508-mm), most fish harvested are sexually immature. With the current size selectivity of the fishery, the spawning stock biomass of permit could decrease quickly in response to moderate levels of fishing mortality; thus, the regulations in place in Florida to restrict harvest levels appear to be justified.

River salinity variations in response to discharge: examples from the western United States during the early 1900s

Major controls on river salinity (total dissolved solids) in the western United States are climate, geology, and human activity. Climate, in general, influences soil-river salinity via salt-balance variations. When climate becomes wetter, river discharge increases and soil-river salinity decreases; when climate becomes drier river discharge decreases and soil-river salinity increases. This study characterizes the river salinity response to discharge using statistical-dynamic methods. An exploratory analysis of river salinity, using early 1900s water quality surveys in the western United States, shows much river salinity variability is in response to storm and annual discharge. Presumably this is because river discharge is largely supported by surface flow.

On the identification of the early developmental stages of clupeiform fishes from Indian waters

Among about 65 species of clupeiform fishes present in the seas around India, most are distributed along both the east and west coasts and have similar spawning seasons and spawning grounds. The most difficult problem experienced with regard to the identification of their early developmental stages is the overlapping sets of diagnostic features among many species within the same genus as well as among species belonging to two or more genera. From studies carried out recently, the present paper sites a few instances wherein certain subtle characters have been used for distinguishing the early developmental stages of a few allied species.

Early and better maturity in carp brood stock with control of environment and feeding

Available of carp breeders in their prim state of maturity is a major constraint in hypophysation. Experiments conducted in a fish farm at Naihati, West Bengal, for two consecutive years, 1983-84 and 1984-85, clearly prove that by manipulation of environmental parameters such as metabolites, dissolved oxygen, running water conditions, as also of stock densities and quality and quantity of feed. Catla catla, Hypophythalmichthys molitrix, Labeo rohita, Cirrhina mrigala and Ctenopharyngodon idella can be made to attain better maturity and spawning stage much earlier than normal i.e. even in summer months and the entire stock spawned during the period from March to September. Percentage of successful breeding, quantities of eggs released and fertilised in relation to the body weight of all the species, were also found to be more in comparison to the brood stock raised through the conventional methods.

Early developmental stages of two Secutor species (Family: Leiognathidae) collected from the Bak-khali river estuary of the Bay of Bengal, Bangladesh

Early developmental stages of two Secutor species, Secutor insidiator (Bloch) (11.9-36.0 mm standard length, SL) and Secutor ruconius (Hamilton-Buchanan) (14.0-33.0 mm SL) collected by ichthyoplankton net from the Bak-khali river estuary of the Bay of Bengal, Cox's Bazar, Bangladesh are described and illustrated. All of the fins with supporting spines and rays were present in the smallest collected sizes of both species. With growth of the specimens, significant changes in melanophore patterns were found. S. insidiator is similar to S. ruconius in having upward protracting mouth parts and body colouration, but can be distinguished easily by its more elongate body shape (body depth 38-47% of SL compared with 46-52% of SL in Secutor ruconius). Both the species occurred round the year from August 1998 to July 1999. The surface water temperature and salinity during the study period varied from 22.0-32) C and 10-37 ppt respectively.

Early developmental stages of Nandus nandus (Ham.)

In order to study the early developmental stages of Nandus nandus an experiment was conducted, where eggs and milt were obtained from the laboratory reared N nandus by stripping after 15 hours of 150 mg/kg body weight of carp PG extract injection. Then the eggs were fertilized in the laboratory and subsequent developmental stages were studied. First cleavage (two cell), four cell, eight cell, sixteen cell and multi cell stages were found 30, 50, 70, 105 and 160 minutes after fertilization respectively. Morula, early gastrula, middle gastrula, late gastrula and yolk plug stages were found 5, 8, 9, 11 and 13 hours after fertilization respectively. Hatching occurred within 20±2 hours after fertilization, and larvae were measured 1.60 mm in diameter. After one hour of hatching two melanophore bands were found at the caudal region of the body of the larvae. Eyes were first observed in l 0 hours, pectoral and pelvic fin buds appeared in 22 hours and well developed in 38 hours old larvae. Mouth cleft and brain lobes were visible when the larvae were 34 and 38 hours old respectively. Myomeres partially appeared in 16 hours, which were clearly visible in 74 hours old larvae. Larvae started wandering and searching for food after 56 hours of hatching. The yolk sac was completely absorbed when larvae became 62 hours old.

Chemical changes in skin mucin as an index of early stages of spoilage in fish

Results of a preliminary investigation on the overall chemical nature of fish skin mucin in lung fish, Clarias batrachus, with special reference to water soluble low molecular weight compounds, are presented. Changes observed during room temperature spoilage have been studied with a view to present a new approach towards the assessment of freshness in fish inspection. pH of the mucin was distinctly alkaline (8.2) and remained unchanged during spoilage. Much of the nitrogen was found to be present in the glycoprotein fraction. Free amino acids and purine bases were present in appreciable quantities in the aqueous extracts which registered a significant increase after 10 hrs. Post-mortem increase in total solids was accompanied by a slight rise in protein nitrogen which may indicate tissue breakdown. Increase in TVN was also observed to occur earlier in the outside mucin as compared to the inside muscle. Presence of free sugars or sialic acid could not be confirmed nor was there any indication of cholesterol and lipoid material as stated in earlier literature.

Studies on the toxicological effects of Asulox-40 and Emisan-6 to eggs and early life history stages of Sarotherodon mossambicus

Toxicological effects of Asulox-40 and Emisan-6 to eggs and early life history stages of Sarotherodon mossambicus were reported. 80% of egg hatching occurred in the controls, 1 p.p.m and 5 p.p.m concentrations of Asulox-40. 10 p.p.m. and 50 p.p.m. concentrations of the same toxicants had 70% and 60% hatchings while in Emisan-6 in the same concentrations the hatching were 70% and! 40%. In 100 p.p.m. concentration of both toxicants 20% incomplete hatching occurred. In Emisan-6 Lc 50 and Lc 100 values were recorded at 32 hand 96h respectively in 10 p.p.m. concentrations. In Asulox-40 the same values were recorded in 24h and 40h respectively at 50 p.p.m. concentration. The fish activity during the experimental period showed initial hyper activity. It was established that the Emisan-6 is more harmful to S. mossambicus than Asulox-40. The harmless concentrations of these chemicals were 1.2 p.p.m. for Asulox-40 and 0.6 p.p.m. for Emisan-6.

Physiological and biochemical study of over-ripened oocyte in the cultivated Caspian brown trout (Salmo trutta caspius) and determination of egg quality markers

To determine the best time for egg stripping after ovulation and over-ripened oocyte in the Caspian brown trout (Salmo trutta caspius), the eggs were retained in the parental abdominal cavity for 40 days post-ovulation (DPO) at 7±0.6°C. Eggs were stripped every 10-day interval in 4 treatment and were fertilized with a pool of semen obtained from 8 males. Also, the physiology and biochemistry of the eggs and ovarian fluids were studied. Results showed that the level of eyed eggs and hatched alevins declined with over-ripening time: that is, the expected amounts (90.65 ± 6.28% for eyeing and 86.33 ± 6.82% for hatching) in newly ovulated eggs (0–10 DPO) decreased to 0.67 ± 1.34% and 0.49 ± 0.98%, respectively, in over-ripened eggs (30–40 DPO). However, larval abnormalities remained constant for 30-days after ovulation. During the course of oocyte over-ripening, the pH of the ovarian fluid significantly decreased and the concentration of glucose, protein, calcium, iron, and aspartate aminotransferase activity significantly increased. Moreover, the concentration of protein, triglycerides, and aspartate aminotransferase activity in the eggs also changed. In the newly ovulated egg, the yolk consisted of homogenous tissue and its perivitelline space diameter had no considerable differences. With over-ripening, the yolk became heterogeneous, while chorion diameter and micropyle did not change. The perivitelline space diameter varied among different areas. The present study demonstrated that the best time to take Caspian brown trout eggs after ovulation at 7± 0.6°C was up to 10 DPO. Among the studied parameters of the egg and ovarian fluid, egg quality was related to both ovarian fluid parameters (e.g., pH, protein, aspartate aminotransferase, glucose, cholesterol, triglycerides, calcium, iron) and egg parameters (e.g., cholesterol, triglycerides, iron, aspartate aminotransferase). Thus, these parameters can be used as a egg quality markers in this species.