Culture suitability of two exotic catfishes (Clarias gariepinus and Pangasius hypopthalamus) with an indigenous catfish (Heteropneustes fossilis)

Culture experiment of African catfish ( Clarias gariepinus) and Thai pangas (Pangasius hypopthalamus) with indigenous stinging catfish (Heteropneustes fossilis) was conducted in the laboratory. The study was conducted for two experiments, where C. gariepinus and P. hypopthalamus were used separately with H fossilis for a duration of 2l days with three feeding treatments, viz. Tubificid worms (T1), SABINCO feed (T2), and no supplemental feed (T3). In experiment 1, the initial length and weight of 4.4cm and 0.60g of C. gariepinus became 6.74cm and 2.33g when fed Tubificid worms, 7.07cm and 2.84g when fed SABINCO feed in the treatment without supplemental feed the final length and weight were 3.67cm and 0.3lg at the end of 21 days of trial. The final length and weight of H fossilis reached 4.55cm and 0.53g from the initial 3.3cm and 0.25g under the treatment fed Tubificid worms while those fed SABINCO feed showed a length and weight of 4.37cm and 0.45g respectively. However, both the initial length and weight were reduced to 2.85cm and 0.12g respectively in the treatment without supplemental feed. In experiment 2, the initial length and weight of 4.37cm and 0.57g of P. hypopthalamus became 5.57cm and 0.57g when fed Tubificid worms, 4.85cm and 0.82g when fed SABINCO feed in the treatment without supplemental feed the final length and weight reduced to 3.95cm and 0.34g at the end of 21 days of trial. The final length and weight of H fossilis reached 5.19cm and 0.82g from the initial 3.25cm and 0.20g under treatment fed Tubificid worms while those fed SABINCO feed showed the final length and weight of 4.93cm and 0.70g respectively. And both the initial length and weight were reduced to 3.07cm and 0.04g respectively in the treatment without supplemental feed. No predatory effect of C. gariepinus and P. hypopthalamus on H fossilis was observed in the experiments.

Production of triploid African catfish, Clarias gariepinus (Burchell), using chromosome manipulation techniques

An experiment was conducted to induce triploidy in African catfish, Clarias gariepinus, using heat shock and cold shock techniques. Cold shock at a temperature of 0± 1°C and 5±1°C for a duration of 15, 30, 45 and 60 min and heat shock at a temperature of 40±0.5°C and 41 ±OS C for a duration of 1, 2 and 3 min was given to induce triploidy 5 min after fertilization. Maximum percentage of triploids (91.4%) were obtained in the heat shock at a temperature of 40±0SC for a duration of 1 min whereas cold shock at 0± 1 C for a duration of 60 min yielded 90% of triploids. Chromosome analysis revealed that diploids have 54 chromosomes and triploids have 81 chromosomes. The erythrocyte measurements of the minor axis and major axis were 1.17 times larger in treated fish than in controls. The growth studies showed that the growth rate was not significantly affected in triploids.

Comparison of effectiveness of heat and cold shocks applied in the induction of gynogenesis in Clarias gariepinus (Burchell)

An experiment was conducted to optimize the procedure of gynogenesis in African catfish, Clarias gariepinus by suppressing meiotic and mitotic cell divisions in fertilized eggs. Gynogensis was conducted by fertilizing normal eggs with UV-irradiated sperm followed by either heat or cold shocking Irradiation of spermatozoa was given for a duration of 1 min and the eggs were fertilized in vitro. Cold shock at a temperature of 3± 1°C for a duration of 30 and 60 min and heat shock at a temperature of 39± 1°C for a duration of 1 and 2 min was applied to induce diploidy. Higher percentage of hatching (68.66) was observed for meiotic gynogens at a shock temperature of 39± 1°C for a duration of 1 min, 5 min after fertilization (af). Higher percentage of mitotic gynogenetic induction (15.33) was observed at a temperature shock of 39± 1°C for a duration of 1 min, 30 min af.

Growth performance and nutrient utilization of catfish Clarias gariepinus fed varying inclusion level of fermented unsieved yellow maize

This study was conducted using 150 fish of Clarias gariepinus to investigate the growth performance and nutrient utilization of Clarias gariepinus fed five treatment diets containing varying inclusion level of fermented unsieved maize. The diets were grouped into CT, T1, T2, T3, and T4 with inclusion levels of 25%, 50%, 75%, and 100% of fermented unsieved maize respectively. Highest weight gain was recorded in T4 with value of 10.24 and lowest weight was recorded in CT with 9.17. High FCR were observed in T2 with value of 0.70 and lower value was observed in T4 with value of 0.62. While, T2, T3, and T4 have highest survival rates with values of 90% in each treatment CT and T1 recorded 80% and 70% respectively. There was a significant (p< 0.05) difference between the food conversion ratios treatment T4 with the best value and other treatments. There was a significant (p< 0.05) difference between the levels of fermented unsieved maize inclusion and the specific growth rate of the experimental fish. The highest value of protein level and feed efficiency were observed in T4 at significant difference level (p< 0.05) than other treatments. It was concluded that fermentation of maize in fish feed has positive effects on the nutritional value of the feed. It is recommend that fermented maize can replace raw maize in fish feed diet for growth performance. KEYWORDS: Fermentation, yellow maize, Clarias gariepinus, Fish, Feed.

Growth performance and nutrient utilization of catfish Clarias gariepinus fed varying inclusion level of fermented unsieved yellow maize

This study was conducted using 150 fish of Clarias gariepinus to investigate the growth performance and nutrient utilization of Clarias gariepinus fed five treatment diets containing varying inclusion level of fermented unsieved maize. The diets were grouped into CT, T1, T2, T3, and T4 with inclusion levels of 25%, 50%, 75%, and 100% of fermented unsieved maize respectively. Highest weight gain was recorded in T4 with value of 10.24 and lowest weight was recorded in CT with 9.17. High FCR were observed in T2 with value of 0.70 and lower value was observed in T4 with value of 0.62. While, T2, T3, and T4 have highest survival rates with values of 90% in each treatment CT and T1 recorded 80% and 70% respectively. There was a significant (p< 0.05) difference between the food conversion ratios treatment T4 with the best value and other treatments. There was a significant (p< 0.05) difference between the levels of fermented unsieved maize inclusion and the specific growth rate of the experimental fish. The highest value of protein level and feed efficiency were observed in T4 at significant difference level (p< 0.05) than other treatments. It was concluded that fermentation of maize in fish feed has positive effects on the nutritional value of the feed. It is recommend that fermented maize can replace raw maize in fish feed diet for growth performance.

A survey of pest (Dermestes maculatus) infestation of dried preserved Clarias gariepinus: its effect on nutrient quantity and quality

Studies on the nutrient composition of smoked-dried Clarias gariepinus were carried out, to assess the effects of pest infestation on the dried catfish from two different market (Abakpa and Kpirikpiri market) area in Abakaliki Metropolis, Ebonyi State, Nigeria. Fish samples from the two markets where kept for pest to infest it and it were taken to Biochemistry Laboratory of Food Science and Technology in Ebonyi State University to assess the damage of pest on dried catfish using standard methods. There was a significant different among the mean weight of fish from the two markets (Abakpa and kpirikpiri; 19.13kg and 18.98kg respectively) compared with the mean weight of the control 20.09kg. There was a variation in the proximate composition of the fishes from the two markets compared with the control, the decreases of the proximate composition are: crude protein from 60.07% to 40.27%; fat from 8.35% to 5.90%; moisture content from 11.67% to 10.67; fiber from 12.93 to 11.93; ash from 4.02% to 3.54% and carbohydrate 12.67% to 5.89%. The longer the storage periods of the infested smoked fish the more the tissue was degraded. Proper handling and storage of dried catfish are required to prolong the shelf life and nutrient content of dried catfish.

Heavy metal concentrations in some organs of African catfish (Clarias gariepinus) from Eko-Ende Dam, Ikirun, Nigeria

The contents of the heavy metals Fe, Zn, Cu, Mn, Pb, Cd and As were examined in the liver, heart, gills, kidney and muscles of the fish Clarias gariepinus from Eko-Ende dam in Ikirun, the capital of Ifelodun Local Government of Osun State, Nigeria. C. gariepinus is the fish of choice and the most demanded in the southwest of Nigeria. The highest metal concentrations were in the liver and the gills while the lowest was in the muscles. The general deceasing order of metal accumulation in the organs was Fe > Zn > Cu > Mn > Cd. Lead and arsenic were not detected in any organ. The values were of lower concentrations than found in many other dams and rivers in Nigeria and some other countries. The values were also lower than the FAO/WHO recommended maximum limits in fish samples, making the fish to be safe and not of any hazards for the consumers.

Studies on induced breeding of Clarias gariepinus (Burchell, 1822) in hapa pens

Induced breeding of Clarias gariepinus was conducted monthly in hapa pens, set up in Otamiri river for nineteen months (June 1993 - December 1994). Results of natural fertilization were unsatisfactory as few eggs were fertilized. Mean relative fecundity, percentage fertilization, percentage hatching and percentage fry survival were: 15.86 ± 1.95 x 10', 18.92 ± 5.28%, 13.50 ± 3.8% and 6.42 ± 0.72%. Results from artificial fertilization were as follows: Mean relative fecundity, 13.80 ± 2.85 x 10', percentage fertilization, 81.91 ± 2.28%, percentage hatching, 86.10 ± 2.46% and percentage fry survival, 21.40 ± 1.89% respectively. The success of artificial fertilization depended largely on the latency period of 9-11 hours and this suggests that induced breeding in pens is feasible. The poor results from natural fertilization were attributed to lack of adequate substrate for the male fish to display courtship and subsequent fertilization of eggs.

Production efficiency in catfish (Clarias gariepinus) Burchell, 1822 in Cross River State , Nigeria

In the study, the production efficiency of catfish in Cross River State was determined. Data was obtained from 120 fish farmers were randomly selected from Cross River Agricultural Zones, using a multistage random sampling technique. Multiple regression analysis model was the main tool of data analysis where different functions were tried. The results indicated that Cobb-Douglass production function had the best fit in explaining the relationship between output of catfish and inputs used, the coefficient of multiple determinant (R2 = 0.61) indicates that sixtyone percent of the variability in output of catfish is explained by the independent variables. The results also indicate that farmers’ educational level positively influence their level of efficiency in catfish production in the study area. The F-value of 16.427 indicates the overall significance of the model at 1 percent level, indicating that there is a significant linear relationship between the independent variables taken together and the yield of catfish produced in Cross River State. The marginal value products of fish pond size (farm size), labour and feed (diet) were N67.50, N 178.13 and N 728.00 respectively, while allocative efficiency for (farm size), labour and feed (diet) were (0.09 over utilized, 2.85 under utilized and 0.99 over utilized), respectively, there existed allocative in-efficiency, there is a high potential for catfish farmers to increase their yields and income. Based on the findings of this study, it is recommended that fish farmers should expand fish farms, improving on production efficiency and adopting new technologies. Regular awareness campaign about new technologies in fish farming should be embarked by extension agents to make fish farmers know the importance of adopting new technologies. KEYWORDS: Production efficiency, Catfish, Cobb-Douglass, Production function, Cross River State

Evaluation of processed groundnut (Arachis hypogaea) cake meals in the feeding of Clariid catfish, Clarias gariepinus, fingerlings

Groundnut cake (GNC) meal is an important source of dietary protein for domestic animals with a cost advantage over the conventional animal protein sources used in aquaculture feed production. It would be useful to evaluate the effects of GNC processing methods on the density and nutritional values of processed GNC meals. The use of processed GNC meals in the diets of Clarias gariepinus fingerlings was evaluated. Seven iso-proteic and iso-caloric diets were formulated, replacing fish meal with roasted and boiled GNC meals, each at three inclusion levels of 30%, 35%, and 40%. Diet I is 100% fishmeal, Diet II is 30% roasted GNC meal, Diet III is 35% roasted GNC meal, Diet IV is 40% roasted GNC meal, Diet V is 30% boiled GNC meal, Diet VI is 35% boiled GNC meal and Diet VII is 40% boiled GNC meal. Results showed that the crude protein content of GNC meals was 40.5% and 40.8% in boiled and roasted GNC meals respectively; the lower protein content for processed GNC meals might be due to heat denaturation of the seed protein, with boiled GNC meal being more adversely affected. The mean weight gain of fingerlings fed roasted GNC meals ranged between 5.29 – 5.64 while for boiled GNC meals, it was between 4.60 – 5.22. Generally, fish performed better when fed diets containing roasted GNC meals, than boiled GNC meals, and compared favorably with fish fed fish meal based diet. Body mass increase, total feed increase, protein efficiency ratio and specific growth rate by C. gariepinus fingerlings in all diets, showed no significant differences, suggesting that processed GNC meals could partially replace diets for C. gariepinus fingerlings without adverse consequences. This study showed that processed GNC meals could partially replace fish meal up to 30% without significantly influencing fingerling growth and health. It is recommended that the use of fish meal as the main basal ingredient for fingerlings could be discontinued, since GNC meal was a cheaper alternative, and could replace fish meal up to 35%, without any significant adverse effects on the fingerling performance. KEYWORDS: Clarias gariepinus, Fingerlings, Groundnut cake meal, Nutrient utilization, Performance.

Aspects of the fishery and biology of the African catfish Clarias gariepinus (Burchell, 1822) amidst environmental changes in Lake Wamala (Uganda)

Environmental changes are some of the factors that affect fisheries and biological characteristics of fishes. The African catfish Clarias gariepinus (Burchell, 1822) has biological characteristics that enable it to persist under various stressful environmental conditions. However, few studies have examined how the African catfish responds to conditions created by a changing climate. The study examined some of the fishery and biological characteristics of African catfish in Lake Wamala (Uganda) to provide an understanding of their response to changing climatic conditions using data for the period 1950 - 2013. Temperature around the lake increased by 0.02ºC/year since 1980, commensurate with the regional trend, while rainfall was above average since 1996, except in 2004 and 2008. Lake depth was strongly positively correlated with rainfall (r =0.83, n= 6, p<0.05) up to 2000, after which, lake depth decreased amidst increase in rainfall. The contribution of African catfish increased from 20% to 85% and 17% to 78% respectively to commercial and experimental catches respectively between 1975 and 2013 despite the decrease in lake depth. The modal total length, condition factor, food, and fecundity did not change. Only size at first maturity decreased from 37.5 to 30 cm TL in females and 39.5 to 34.2 cm TL in males between 1999/2000 and 2012/2013. The biological characteristics of the African catfish were comparable with those of the same species in other lakes and remained relatively stable. The results suggested that the African catfish has the capacity to persist and/or adjust appropriately under conditions created by climate variability and change, and if properly managed, can sustain the fisheries of Lake Wamala.

Studies on the optimum protein to energy ratio of African catfish (Clarias batrachus Burchell)

A laboratory trial was conducted to determine the optimum dietary protein to energy (P/E) ratio of African catfish, Clarias gariepinus. The experiment was carried out in a flow-through system for 6 weeks. There were 12 treatments each with two replicates having 10 fish each with a mean initial weight of 1.80 ± 0.02g. Twelve semi-purified diets were formulated with four digestible crude protein levels (23, 26.5, 30 and 33.5%) and three digestible energy levels (2.25, 2.75 and 3.25 Kcal/g). The fish were fed three times daily at satiation level. The results of the study showed that, diet containing 33.5% digestible protein and 2.75 kcal/g digestible energy with a protein to energy ratio of 121 .8 (mg protein/kcal) appeared to be best utilized for growth.

Stock assessment of Lates niloticus (L.), Oreochromis niloticus (L.) and Rastrineobola argentea (Pellegrin) using fishery dependent data from the Tanzanian waters of Lake Victoria

Catch data were collected from three beaches in the Mwanza area of lake Victoria, Tanzania for Oreochromis niloticus (L.), Rastrineobola argentea (Pellegrin) and Lates niloticus (L.). Sampling took place in October 1997 and February, June and September 1998. The CPUE for O. niloticus was 3.9 to 6kg boat super(-1) and for R. argentea from 98 to 282 kg boat super(-1). There was no obvious trend in catch rates for L. niloticus. The modal length for O. niloticus recorded at Chole beach was 34cm TL. In February, fish were larger (41-45 cm) than in the other surveys. Rastrineobola argentea caught in October 1997 had modal length at 65 mm TL with some smaller fish. In February and June prominent length modes occurred at 45 and 58 mm respectively, which may represent the same cohort as the small fish caught in October 1997. In September 1998, there were two length modes at 46 and 60 mm. The 60 mm fish may represent the same cohort seen in previous surveys, suggesting growth from approximately 30 mm to 60 mm in an eleven-month period. Lates niloticus landed at Kayenze beach over the four surveys had a modal length of 46 cm TL. Fish species encountered on the three beaches during the surveys were O. niloticus, R. argentea, Bagrus docmak Forsskall, Clarias gariepinus (Burchell), Protopterus aethiopicus Heckel, Labeo victorians Boulenger, Synodontis afrofischeri Hilgendorf, Synodontis victoriae Boulanger, Schilbe intermedius (L.), Brycinus jacksonii (Boulanger), Mormyrus kannume Forsskall and Haplochromine cichlids

Growth response, feed conversion rate and cost benefits of hybrid catfish fed maggot meal based diets in outdoor tanks

The growth response, feed conversion ratio and cost benefits of hybrid catfish, Heterobranchus longifilis x Clarias gariepinus fed five maggot meal based diets were evaluated for 56 days in outdoor concrete tanks. Twenty-five fingerlings of the hybrid fish were stocked in ten outdoor concrete tanks of dimension 1.2mx0.13mx0.18m and code MM sub(1)-MM sub(5) in relation to their diet name. Five isonitrogenous and isocaloric maggot meal based diets namely MM sub(1)-0% maggot meal, MM sub(2)-25% maggot meal, MM sub(3)-50% maggot meal, MM sub(4-)75% maggot meal and MM sub(5-) 100% maggot meal were used for the experiment. The higher the proportion of maggot in the meal, the higher the ether extract and crude fiber. No significance difference P>0.05 exists between ash content of the experimental diets. Diet MM sub(2) had the best growth performance and highest MGR with a significant difference P<0.05 with other diets fed fish. No significance differences P>0.05 exists between the growth parameters for diets MM sub(1), MM sub(3), and MM sub(4). A positive correlation (r=1.0) exists (P<0.05, 0.25) between the growth parameters for the different experimental diets. Highest correlation r super(2)=0.9981 exists P<0.05 between MGR within the treatments. However, there no significant (P>0.05) difference in expenditure but there is between the profit indices and incidence of cost between the trials. MM sub(2) has the best yield cost and net profit. Without any reservation, inclusion of maggot based meal diet is recommended as feed of hybrid catfish to 75% inclusion for growth and profit incidence

Growth performance of Heteroclarias fed maggot meal at varying inclusion levels

70-day growth trial was conducted with Heteroclarias: Heterobranchus bidorsalis X Clarias gariepinus (mean weight 0.64~c0.006g) fed diets based on various inclusion levels of Maggot Meal. The fishmeal in the control diet was replaced with maggot meals at 25%, 50%, 75% and 100% levels to supply 40% crude protein in the final diets. The trails were conducted in glass tanks (60cmx30cmx30cm). Evaluation of growth parameters and nutrient utilization of the fish was based on weight gains, protein intake, protein efficiency ratio, net protein utilization, feed conversion efficiency and carcass analysis. Best growth and feed conversion efficiency were obtained with the 75% dietary inclusion of maggot meal. There was no significant differences (P>0.055) between the group of fish on 50% and 75% dietary inclusion maggot meal in growth performance and protein efficiency ratio but, there was a significant (P<0.05) difference in the NPU (Net Protein Utilization) and protein gain between the control diet and those fed on maggot meals. There was no marked variation in the survival rate of fish on all diets