Protocols for conducting dolphin capture-release health assessment studies

Marine mammals, such as dolphins, can serve as key indicator species in coastal areas by reflecting the effects of natural and anthropogenic stressors. As such they are often considered sentinels of environmental and ecosystem health (Bossart 2006; Wells et al. 2004; Fair and Becker 2000). The bottlenose dolphin is an apex predator and a key component of many estuarine environments in the southeastern United States (Woodward-Clyde Consultants 1994; SCDNR 2005). Health assessments of dolphins are especially critical in areas where populations are depleted, show signs of epidemic disease and/or high mortality and/or where habitat is being altered or impacted by human activities. Recent assessments of environmental conditions in the Indian River Lagoon, Florida (IRL) and the estuarine waters surrounding Charleston, South Carolina (CHS) highlight the need for studies of the health of local bottlenose dolphins. While the condition of southeastern estuaries was rated as fair in the National Coastal Condition Report (U.S. EPA 2001), it was noted that the IRL was characterized by poorer than expected benthic communities, significant sediment toxicity and increased nutrient concentrations. Similarly, portions of the CHS estuary have sediment concentrations of aliphatic aromatic hydrocarbons, select inorganic metals, and some persistent pesticides far in excess of reported bioeffect levels (Hyland et al. 1998). Long-term trends in water quality monitoring and recent scientific research suggest that waste load assimilation, non-point source runoff impacts, contaminated sediments, and toxic pollutants are key issues in the CHS estuary system. Several ‘hot spots’ with high levels of heavy metals and organic compounds have been identified (Van Dolah et al. 2004). High concentrations of anthropogenic trace metals, polychlorinated biphenyls (PCB’s) and pesticides have been found in the sediments of Charleston Harbor, as well as the Ashley and Cooper Rivers (Long et al. 1998). Two superfund sites are located within the CHS estuary and the key contaminants of concern associated with these sites are: polycyclic aromatic hydrocarbons (PAH), lead, chromium, copper, arsenic, zinc and dioxin. Concerns related to the overall health of IRL dolphins and dermatologic disease observed in many dolphins in the area (Bossart et al. 2003) initiated an investigation of potential factors which may have impacted dolphin health. From May-August 2001, 35 bottlenose dolphins died in the IRL during an unusual mortality event (MMC 2003). Many of these dolphins were diagnosed with a variety of skin lesions including proliferative ulcerative dermatitis due to protozoa and fungi, dolphin pox and a vesicular dermatopathy of unknown etiology (Bossart et al. 2003). Multiple species from fish to dolphins in the IRL system have exhibited skin lesions of various known and unknown etiologies (Kane et al. 2000; Bossart et al. 2003; Reif et al. 2006). On-going photo-identification (photo-ID) studies have documented skin diseases in IRL dolphins (Mazzoil et al. 2005). In addition, up to 70% of green sea turtles in the IRL exhibit fibropapillomas, with the highest rates of occurrence being seen in turtles from the southern IRL (Hirama 2001).

Boundary options for a research area within Gray's Reef National Marine Sanctuary

Gray’s Reef National Marine Sanctuary (GRNMS) is exploring the concept of a research area (RA) within its boundaries. The idea of a research area was first suggested in public scoping meetings held prior to the review of the Gray’s Reef Management Plan. An RA is a region specifically designed for conducting controlled scientific studies in the absence of confounding factors. As a result, a multidisciplinary group gathered by GRNMS was convened to consider the issue. This Research Area Working Group (RAWG) requested that a suite of analyses be conducted to evaluate the issue quantitatively. To meet this need, a novel selection procedure and geographic information system (GIS) was created to find the optimal location for an RA while balancing the needs of research and existing users. This report and its associated GIS files describe the results of the requested analyses and enable further quantitative investigation of this topic by the RAWG and GRNMS.

Studies on the occurrence of coliforms and streptococci in coastal waters of Bombay

The distribution of coliforms and streptococci in Bombay coastal waters was investigated in order to determine the state of pollution in the area. Findings show the incidence of pollution to be of a recurring nature, primarily due to discharge of raw or improperly treated sewage; thus regular monitoring of the incidence of coliforms and streptococci is of importance in determining the public health safety of the beaches and coastal waters.

Studies on selected biological parameters off Mithapur (Gujarat)

The biological characteristics off Mithapur, India, indicated fairly high productivity in terms of macrobenthos and phytoplankton pigment concentration. The area sustained low standing stock of zooplankton. The overall biological productivity of plankton and macrobenthos indicated 30-90% reduction from the premonsoon to postmonsoon period. This decline in the standing stock of plankton and benthos was coinciding with the peak fishing season of the area.

Limnological studies of a freshwater tropical impoundment : Powai Lake. 1: Morphometry and physical features

Powai Lake, an impoundment, came into existence in 1891 when the riverlet Dhanisar was dammed to conserve rainwater for drinking purpose. However, the water was found to be unpotable and the lake was leased out to the Angling Association, Bombay, exclusively for angling and sports. The lake is located about 27 km in the northeast of Bombay city at a height of 55m above MSL. It is rainfed with an average rainfall of 2,400 mm. The maximum waterspread area is 220 ha with a maximum capacity of 8.11 million m super(3) in the peak monsoon period when the water overflows the dam. There is no drawdown from the lake. Fluctuation in the water level is mainly due to evaporation and percolation. Transparency is low mainly due to suspended organic particles. There is hardly any difference in the water temperatures of surface and bottom, hence the annual heat budget is low at 2,818 cal m super(-2).

Hydrological studies of Matatilla Reservoir, Uttar Pradesh

Matatilla Reservoir, located in semi arid region, (Lat. 25 degree 15'N and Long. 78 degree 23'E) has an area (at FRL) of 13,893 ha, volume and shore development 0.663 and 1.65, shoreline 73.6 km. Volume and shore development indicate that greater part of the reservoir is shallow, which is a favourable point for fish productivity. Temperature and dissolved oxygen gradually decreased with the increase in depth. Carbon dioxide was absent from the surface but invariably present in the bottom (3.6 ppm) pH remained alkaline (7.2-8.4 ppm) throughout the year. Alkalinity, chloride, calcium, magnesium, hardness and priductivity was maximum in pre-monsoon and minimum in monsoon except for calcium and manganesium in post-monsoon. Phosphate, nitrogen and ammonical nitrogen were found in traces. These variations may be due to influx and outflow of water and use of reservoir water for multipurpose activities.

Rotifera from Sri Lanka (Ceylon) 2. Further studies on the Eurotatoria including new records

In the first paper of this series, Chengalath and Fernando (1973) dealt with the genus Lecane from Sri Lanka. In all, twenty five species were recorded, of which seventeen species were new records. Two new species were also described. On the present paper the authors deal with the rest of the Eurotatoria. Again they have found many new records. In all 79 species are described in the present paper of which 47 are new records. The present study is based on the examination of over 300 samples from 135 localities including large and small tanks, ponds of various sizes, rice fields, streams and marshes. The collections cover the whole area of Sri Lanka and were taken during different seasons of the year mainly from 1968-1972.

Intertidal transect studies of northern Monterey Bay

In accordance with a contract dated 10/22/71 between the Association of the Monterey Bay Area Governments (AMBAG) and the University of California, Santa Cruz, (UCSC), two permanent intertidal transects with 14 permanent meter-square quadrats were established on the north shore of Monterey Bay during November, 1971. One transect (6 quadrats) was placed on the shore near the Santa Cruz Sanitation outfall, while the second (8 quadrats) was placed near the Eastcliff Sanitation District outfall at Soquel Polnt (Pleasure Point). Animals and plants within the quadrats were listed, their abundance estimated, and representative specimens collected for a reference collection maintained at UCSC. Additional species of animals and plants in the areas of the transects were collected for the reference collection. These collections will serve as a base-line for comparative studies which can follow the magnitude and direction of future changes in these areas.

Studies on morphometry and hydrology of Gandhisagar Reservoir with special reference to its fisheries

Gandhisagar, the second largest reservoir of India is located in Mandsaur district of Madhya Pradesh at latitude 24°44'N and longitude 75°33'E at an altitude of 403.56m MSL in orientation from NE to SE. It has an extensive water spread area of 66000 ha at full reservoir level with a maximum and mean depth of 49.52 and 11.73 m respectively. The maximum length and width of the reservoir are 112 and 16km having a total shore line of 442km. Details of catchment area, bathymetry, standard hydrological data giving water level relation of the basin to water spread area, volume and fish production and the bottom topographical details of 11 experimental fishing stations and 6 fish landing centres are discussed.

Studies on the Tetraclitidae (Cirripedia: Balanomorpha); new species of Tetraclita from the Red Sea

The tetraclitid fauna at Elat, Israel, on the Gulf of Aqaba, Red Sea, consists of three morphologically distinct species. Tetraclita rufotincta Pilsbry, 1916 was reported previously from this region and may have been confounded with T. achituvi n.sp. and T. barnesorum n.sp. Although these species occur sympatrically, according to previous studies, and recent observations, they apparently occupy different levels in the narrow intertidal zone (=50 cm) of this area. Cirral morphology suggests that each differs in the manner by which they capture and manipulate prey. Little is known about the occurrence of these new species elsewhere in the Red Sea and adjoining Arabian Sea.

Studies on distribution, diversity and production of macrobenthic fauna in the Chahbahar Bay

The population density, distribution, diversity and secondry production of macrobenthic fauna of the inner Chahbahar Bay were studied through bi-monthly sampling from April 1995 to March 1996. Samples were collected from water near the bottom and sediment at 14 stations inside the Bay and one reference station located outside at the entrance to the Bay. The environmental parameters Such as temperature, water depth, salinity, pH and dissolved oxygen as well as percentage silt-clay and total organic matter of the sediment were measured. The faunal population density and their distribution is discussed in relation to the environmental changes. results obtained indicated both spatial and temporal heterogeneity in faunal distribution of the Chahbahar Bay. The total of 18 groups of macrofauna were identified in all samples. Amphipods formed the dominant group (21%) followed by polychaetes (19%), gastropods (15.7%) bivalves (10.6%) and all other groups (33.7%). Seasonal changes in faunal density is shown in relation to Indian Ocean southwest monsoon,the result of which indicated lower population density during monsoon (June to September) than that of the premonsoon (February to May) and post monsoon (October to January) periods. The numerical abundance of macrobenthos varied from 10260.m2 before monsoon to 5190 m2 during monsoon season. Three dominant groups of macrofauna including polychaetes, gastropods, and bivalves were identified in all collected samples. Indices of diversity, richness and evenness were calculated for these three dominant groups. The Shannon-Weaver information index was used to describe the spatially and temporally variation in diversity of these three major faunal groups. The results exhibited lower faunal diversity during monsoon period. The annual production of two dominant macrofauna species including a species of bivalve, Nuculana acuta and a species of Cephalochordata, Branchiostoma lanceolatum were measured by using age group determination. Furthermore the mean biomass and total annual production of macrobenthic fauna were estimated for the whole studied area. The potential yield of demersal fishery resources (fish and crustacean) then estimated and worked out to be 15360 tons/year asuming 10% ecological efficiency of hypothetical pyramid from 3rd to 4th marine trophic level. Accordingly the annual exploitable demersal fishery resources for the entire Chahbahar Bay was estimated to be 7600 to 8500 tons/year by taking 50 to 55% of the total estimated potential in to account.

Studies on Mahshahr Creeks macrobenthos with emphasis on their importance in fish feeding

To study the macrobenthic community at Mahshahr creek four Creeks namely Bihad, Doragh, Ghazaleh and Ghanam were chosen. Sampling was conducted on bimonthly basis and carried out from August 1996 to June 1997, 216 sediment samples were collected from 12 stations using 0.1 m^2 Van Veen Grab, The stations were located at the mouth, middle and the end of each Creek. In situ measurements of temperature pH, DO and salinity were done using different sensors. The samples for the measurements of TOM, grain size were collected and analysed in vitro. The results indicate spatial and temporal heterogeneity in the structure of macro faunal assemblages of the creeks. A total of 12 macrofaunal groups were identified within the study area. Amphipods were the most dominant group (43%) followed by polychaetes (42%), copepods (3.5%), tanaids (3.1%) and other groups (8.4%). The range for the numerical abundance of macrobenthos was between 12583 to 3648 individual per m2 and the variation was done to different bottom texture the variable environment conditions governing the different parts of each creek as well as within creeks. Application of diversity indices (Shannon H and Simpson indices) on the dominant macrobenthic assemblages (crustaceans & polychaetes) was varied between 1 to 2.5 being higher in Bihad and Ghanarn and much reduced Shannon H index or a higher Simpson in Ghazaleh. Probably brought about activities in this creek. Gut content analysis of four species of fish showed that the main food items consist of Crab, Shrimps and other crustacean species, The secondary production of macrobenthic fauna and hence a fish production were assessed. To do this first the production of most dominant species Apseudes sp. was computed through Cohort analysis. The total macrobenthic production was estimated and from this fish production was computed. The macrobenthic and fish secondary productions were 24300 tons/year) and 24300 (tons/year) respectively. These values were lower than those with similar areas in the Indian Ocean.