Studies on the chemical control of psychrophilic bacterial spoilage of fish. 4 - The effect of chemical preservatives on the growth of psychrophilic bacteria isolated from marine fish

Dehydroacetic acid and ammonia were found to be very effective in checking the growth of all the cultures at all concentrations tried. The two nitrofuran derivatives namely, semicarbazone and AF-2 were fairly effective, semicarbazone being more effective than AF-2. Sodium nitrite was found to be totally ineffective against the cultures at all concentrations tried.

Experiments with fibre-glass sheathing as a protection against marine wood-boring organisms

Wooden test panels of different species of wood sheathed with fibre-glass reinforced plastic were subjected to immersion tests along with corresponding controls in Cochin Port area and the effect of the protective coating studied.

On the behaviour of marine crustaceans in an electrical field of alternating current

The minimum flow of current essential to produce electro-narcosis and fixation in marine crustaceans, Metapenaeus affinis, Parapenaeopsis stylifera, Panulirus ornatus and Neptunus sanguinofentus in an AC field of specific strength varied with the lengths of animals tested, except in case of the last one where the increase in size of animals showed no significant effect on the effective period for different reactions. M. affinis in interrupted AC required threshold pulses for narcosis and fixation, which varied inversely with the length of the organism. Animals subjected to current of higher effective period required longer time for recovery in interrupted AC of 50 c/s. The period for narcosis and recovery of M. affinis did not vary significantly with repeated stimulations on the same organism.

Behaviour of marine microflora towards antibiotics 1. Sensitivity towards chlortetracycline (CTC)

The sensitivity of 61 cultures of bacteria isolated from fish towards chlortetracycline (CTC) at 5 ppm and 20 ppm levels has been determined on two solid media: sea water agar (SWA) and a distilled water based medium consisting of peptone, beef extract, glucose and NaCI (PBGA). The cultures employed consisted of (i) gram-negative rods of marine origin (Achromobacter, Pseudomonas, Vibrio and Flavobacterium) and (ii) gram positive organisms (Micrococci and Corynebacterium). Depending on the inhibition zone diameter, the order of CTC sensitivity was found to be Pseudomonas

On the reaction of marine fishes in interrupted A.C. of 50 Hz

The reactions of marine fishes in interrupted A. C. off uniform electrical field and constant current density have been studied in order to determine the optimum effective periods of shock: for narcosis and fixation. Higher effective periods were required with gradual decrease in potential difference between head and tail, which in turn depended on the length of fish and its position in electrical field. The body voltage of fish varied directly with the length and inversely with the rise of angle between their body axis and field lines. The fish subjected to higher effective period took longer time for recovery from narcosis. The effect of impulse current was manifested by increased rate of gill movements in Platax tefora, Haetodon ollaris, Crysophus burda, Siganus vermiculatus and Scolopsis leucotaenia, and lowered rate of movements in others when subjected to different effective periods inspective of length.

Designing of a single pack wash primer for aluminium surfaces in a marine environment

Design aspects and comparative performances of different laboratory formulations of wash primers were studied under laboratory and field conditions with reference to scratch hardness, flexibility, stability, resistance to corrosion and adhesiveness. The different formulations of single pack wash primers tested have shown superiority of the formulation prepared out of “mowital” and that it is comparable in performance to double pack wash primer.

Some bacteria isolated from marine plankton and mud

57 bacterial strains isolated from marine plankton and mud from the inshore waters of Cochin, are described. The flora of the plankton was dominated by chromogens while mud sample and failed to produce chromogens. The different strains are divided into five arbitrary groups for the convenience of description. The flora is similar to that of Mandapam (east coast) while it differed from the flora of Calicut (west coast) in the paucity of Bacillus and other spore formers.

Studies on arsenical creosote as a wood preservative for marine structures. Part 1. Incorporation of arsenic in creosote at various temperatures

A detailed study of the fortification of normal creosote and low temperature creosote with As sub(2) O sub(3) at 40°C, 50°C, 60°C, 70°C, 80°C and 90°C was carried out. When compared to normal creosote, low temperature creosote has been found to combine more easily with As sub(2) O sub(3) when temperature was . raised from 40 to 90°C. The incorporated arsenic values obtained shows that low temperature creosote with high phenolic content, retains considerably more As sub(2) O sub(3) and a maximum of 0.2180% w/w can be incorporated in low temperature creosote at 90°C.

Studies on arsenical creosote as a wood preservative for marine structures. Part 2. Observation on leaching, corrosion and resistance to borer attack

A detailed study on arsenical creosote with reference to leaching, corrosion and anti-borer properties was carried out. Results showed that aging had very little effect on the preservative which suggested better fixation of the preservative into the wood. Corrosion of mild steel, galvanised iron, aluminium-magnesium alloy (M57S) and copper panels in the preservative was found to be negligible. Normal creosote and low temperature creosote of Regional Research Laboratory, Hyderabad, both fortified with arsenic trioxide resisted borer damage on wooden panels for a period of over five months in the port of Cochin. The performance of low temperature creosote fortified with arsenic was found to be equally satisfactory when compared to normal creosote fortified in the same manner. A loading of 208.6 Kgs/ml³ for Haldu (Adina cordifolia) and 138 Kgs/m³ for Mango (Mangifera indica) in the case of normal creosote and 177 Kgs/m³ for Mango the case of RRL creosote were found to be sufficient for treating the wood.

A case history on the phenomenal corrosion of marine propellers in fishing boats

Although high tensile brassess of copper-zinc alloys (Manganese bronze) find extensive use in all marine applications, under aggressive sea-water, marine propellers cast out of such an alloy frequently suffer heavy corrosion damages due to dezincification. An interesting case history where a number of propellers have undergone dezincification in the Cochin backwaters is narrated in this paper.

On the importance of ship-bottom fouling by marine organisms: a techno-economic survey

A high attention has been paid for constant research on the preservation of materials in the marine environment. This includes all phases of design, development, applied engineering and economics which may influence the construction and operation of ships and underwater installations.

Occurrence of Vibrio parahaemolyticus in marine prawns and environments

Qualitative studies on the microflora of slime and guts of prawns and of sea water off Nagapattinam showed the presence of Vibrio in the slime and sea water. They were further tested for Vibrio parahaemolyticus types and related bio-types. Evidence of its occurrence is given. This points to the need for further studies on the distribution of this organism in terms of public health significance.

Development of electro-trawl system in marine environment

High voltage pulsed current produced on board a trawler is fed to electrodes distributed along the foot rope of a trawl net through two core TRS cable which builds up a homogeneous electrical field around the net mouth. By comparative fishing tests with the electrified and non-electrified 32 m long wing trawl net, the increase in total catch of shrimps and fishes was found to be 19.8 and 36%, respectively.

Lipid distribution in body and skin of four species of marine fish

The paper presents results of a study on the analytical characteristics of lipids from the skin and muscle of four important species of Gujarat coast fishes. Changes in characteristics like saponification value, iodine value and unsaponifiable matter of these lipids during different months of the year are also reported.

Nucleotide degradation and production of hypoxanthine in some Indian marine and freshwater fishes

Changes in nucleotides and production of hypoxanthine in rohu (Labeo rohita), mrigal (Cihhrina mrigala) and common carp (Cyprinus carpio) during storage at 2-4°C were examined. Differences were observed between common carp and others. Production of hypoxanthine in pomfret (Stromateus argenteus), cat fish (Arius macronotacanthus), shark (Scoliodon spp.), seer fish (Scomberomorus guttatus), ray fish (Dasyatis imbricata) and prawns (Parapenaeopsis stylifera) was examined during storage at 2-4°C and -28°C. At 2-4°C hypoxanthine increased regularly but at -28°C there was no increase during storage over 28 weeks.