Heavy metals pollution in the coastal environment of Karachi

The marine environment near Karachi, particularly the Baba channel, Chari Kundi channel and Manora channel have been found contaminated with industrial effluents discharged by Malir and Lyari rivers, since they carry a high concentration of toxic heavy metals viz. Pb, Zn, Cu and Mn emanating from the industrial area and are received and discharged by the Lyari river. Out of 60 seawater samples collected from the above mentioned areas, Pb was present in 55 samples and Zn in 58 samples. The concentration of Pb was between 0.04 and 59.2ppm and the concentration of Zn was between 0.05 and 1.9ppm. Similarly all the 60 sludge samples collected from Lyari outfall and its adjoining area have been found to contain Pb and Zn in alarmingly high concentratios, which for Pb was between 15.4 and 3209.9ppm while for Zn was between 87 and 111.3ppm. Cu and Mn were also found in all the above samples.

On the selective action of the cod end meshes of a shrimp trawl

Selectivity studies using cod end and cover to determine the optimum cod end mesh size for commercial size groups of shrimps was carried out at Cochin during 1963-64 fishing season. The normality of the result was checked by trouser cod end method. Although the present investigation was mainly aimed to find out suitable cod end mesh size for commercial varieties of shrimps, five commonly occurring species of fishes were also taken. The 50% escape level, co-efficient of selectivity and selection factor for all the species were worked out. From the findings, the authors stress the necessity of increasing the cod end mesh size from the present condition (25.4 to 31.70 mm) to 41.65 mm fabricated mesh size to avoid depletion of the natural population.

On the relative catch efficiency of different shaped otter boards in bottom trawling

Three different types of otter boards varying in shape were tried to find out their relative catch efficiency. They were operated with two types of nets. The results indicated that the curved otter boards gave more catch per unit effort with all the combinations of nets and riggings tried.

The design and construction of flat rectangular otter boards for bottom trawling: a review

The design and construction of the otter board is a subject of great importance for economy in trawling. This review incorporates a historical resume tracing the change and development• of otter boards. The size of the otter board and its relationship with the horse power of the engine and size of the net and the methods of rigging are dealt with. The factors influencing the horizontal spread are discussed. The effect of the angles of attack, heel and tilt and the ground reaction on the force coefficients have been reviewed and discussed with particular reference to flat rectangular otter boards used for bottom trawling. A short account of other designs of otter boards used for improved efficiency is given. Suggestion for improving the efficacy of otter boards based on the work hitherto done has been made. The contributions relating to the various aspects of design and performance of trawl boards carried out till 1969 have been considered.

A Preliminary study of the defensive spines of some Malayan freshwater fishes

The defensive spines of fifteen Malayan freshwater fishes have been studied morphologically. The classification of spines has been slightly modified from the previous work of Fernando and Fernando (1960). They are divided into simple, denticle-bearing and venom-carrying. The simple spines are further sub-divided into single and multiple and the denticle-bearing into Bagriid and Clariid types. The latter agree morphologically with the venom-carrying spines of previously studied forms and may be a degenerate condition. Simple spines occur singly in the Cyprinidae where they are found at the anterior end of the dorsal fin. A spine of similar structure occurs in the catfish Glyptothorax. In the families Anabantidae, Cichlidae and Mastacenbelidae simple spines occur as a series. Denticle-bearing spines occur in the catfishes (Order-Nematognathi). Those having denticles on one face occur in the Bagridae, Siluridae, Sisoridae, and Akysidae. They are referred to as Bagriid type. In the other type denticles occur on the anterior and posterior faces of the spine. They are referred to as Clariid type. None of the Malayan species studied had venom-carrying spines and they are unlikely to be found in the freshwater species. The functioning of the defensive mechanism whose morphological bases are spines is discussed and the relation between the size and habitat on the effectiveness of the spines is mentioned. The evolution of defensive spines is discussed briefly.

Quantitative survey of Sargassum along the south-west coast of Ceylon

Sargassum is a brown seaweed from which alginic acid is extracted. A survey was carried out off the south-west coast of Ceylon in December 1963 and January 1964 with the view to determining the quantity of Sargassum available.

Results of the Austrian-Ceylonese hydrobiological mission 1970 of the 1st Zoological Institute of the University of Vienna (Austria) and the Department of Zoology of the Vidyalankara University of Ceylon, Kelaniya. Pt. 9. Dytiscidae (Coleoptera)

The intensive collecting of Prof. Dr. F. Starmühlner and Prof. Dr. H. H. Costa in Ceylon in 1970 produced among others some Dysticidae. The material turned out to be especially interesting as it comes all together from running waters, in which otherwise collecting is infrequent. From Sri Lanka quite a lot of species of Dytiscidae are already known.

Studies on the ice storage characteristics of blood clam Anadara granosa meat

Ice-storage study of blood clam (Anadara granosa) meat in direct contact and out of contact (in 200 gauge polyethylene bag) with ice was taken up to assess the amenability of the meat to icing. Changes in moisture, total protein, non-protein nitrogen, α amino nitrogen, total volatile base nitrogen, glycogen, free fatty acid, peroxide value, total bacterial count and coliform count were followed every day. The raw and cooked meat were also subjected to organoleptic evaluation. The study showed that the clam meat can be ice-stored in very good condition out of contact with ice in polyethylene packets for 4 days and in direct contact with ice for 2 days.

Notes on the external sex characters of Chanos chanos Forskal spawners

In the present study, no visible differences between the sexes of C. chanos with reference to external features such as colouration, shape of head, snout and operculum, presence of tubercles or nasal pores, length, size and shape as well as any roughness in the various fins, could be found. However, the anal region of the mature milkfish (sabalo) exhibits discernible anatomical differences in the male and female. The male has two main openings visible externally: the anterior anus and the posterior urogenital opening at the tip of the urogenital papilla. The female has three main openings instead of two: the anteriormost anus, followed by the genital pore and the urinary pore located posterior to the genital pore at the tip of the urogenital papilla. Internal examinations were also made on both sexes. In ripe sabalo, it is easier to distinguish the sexes since milk oozes out of the urogenital pore by pressing the abdomen of the ripe male fish. Gravid females are identified by their distended abdomens.

Impacts of fish sanctuaries on the production and diversity of plankton on beels of haor region in Bangladesh

The experiment was carried out to study the impacts of fish sanctuaries on the production and diversity of plankton in beels of haor region at Mithamain Upazila of Kishoreganj district in Bangladesh during July 2004 to June 2005. A total of 75 (60 phyto and 15 zooplankton) and 74 (59 phyto and 15 zooplankton) genera of plankton were recorded in T-1 and T-2 (with sanctuary) respectively while only 50 (39 phyto and 11 zooplankton) genera were obtained in T-3 (control). Chlorophyceae and Copepoda were the most dominant group of phytoplankton and zooplankton respectively in all the treatments. The total phytoplankton numbers were found to range from 5472 to 35,833 cells/l and 5250 to 40,472 cells/l and total zooplankton from 667 to 1722 cells/l and 611 to 1667 cells/l in T-1 and T-2 respectively in sanctuary sites whereas the ranges of phytoplankton and zooplankton in the control site were 1778 to 29,333 cells/l and 56 to 1056 cells/l respectively. The maximum phytoplankton and zooplankton were recorded during winter season in all the treatments. The ranges of total plankton were 6194 to 37,500 cells/l, 6028 to 41,806 cells/l and 1889 to 29,444 cells/l in T-1, T-2 and T-3 respectively. The phytoplankton, zooplankton and total plankton recorded in treatments with sanctuary were significantly higher (p<0.5) than the treatment without sanctuary (control) indicating positive impacts of sanctuaries on the production of plankton. Between two treatments of fish sanctuaries the total plankton populations were comparatively higher in T-2 than T-1.

Assessment of impact of ten days fishing ban in the major spawning grounds of hilsa (Tenualosa ilisha, Fisher and Bianchi, 1984)

A study was undertaken to find out the impact of ten days fishing ban in the major spawning grounds of hilsa during October to December. The study revealed a positive impact of fishing ban during spawning season on reproduction success of hilsa. Proportionate distribution of male and female hilsa also showed a significant level of distribution in and around the spawning grounds where the ratio was 35:65. Percent of oozing hilsa during fishing ban period in the spawning grounds was observed 1.61%. About 5% spent hilsa was observed in the fish landing centers and was compared with the data of Global Environment Facility (GEF) and Bangladesh Fisheries Research Institute (BFRI) studies and was found about 2.80-3.57 times higher than that of the findings of 2002 and 2003. Estimated egg production value showed about 46,800 Kg of eggs could have been produced that indicating a positive impact of 10 days fishing ban in spawning season. Abundance of higher percentages of gravid hilsa was found which were not available in the same quantity and condition in the non-fishing ban period. During the present investigation, fairly higher amount of spent hilsa and juveniles were also observed in the spawning grounds. Along with the jatka fry, spawn and fries of other fishes were also found in higher quantity than the previous years and thus it is assumed that fishing ban also might have positive impact on the successful breeding of other fishes. Overall, the fishing ban was found effective for successful breeding of hilsa.

Study on the growth and survival of Channa striatus (Bloch) postlarvae using live feed

Feeding experiments were conducted on the postlarvae of Channa striatus with two different live feeds - a copepod (Thermocyclops decipiens) and cladocerans (Moina micrura and Ceriodaphnia comuta) individually and in mixture. Food was provided at the rate of (500±50 Ind/L) and the experiments were carried out in 100 litre capacity tanks for 30 days. Results indicated better weight gain (951.85±28.77%) and survival (92.00<%) of postlarvae fed with mixed live food than individual live feed organisms.

Studies on the growth and production of six major and exotic carps in Nasti baor, Jhenaidah, Bangladesh

An investigation on growth, production and fishery of three Indian major carps: rohu, Labeo rohita, catla, Catla catla and mrigal, Cirrhinus mrigala and three exotic carps: silver carp, Hypophthalmichthys molitrix, grass carp, Ctenopharyngodon idella, and common carp, Cyprinus carpio was carried out in Nasti baor during February to April months. In catch per unit effort (CPUE) study the highest catch/day/person (3.13 kg) and catch/day/gear (40.65 kg) was recorded in the month of March for kochal fishing. In komar fishing catch/day/person (15.08 kg) and catch/day/gear (1206 kg) was also found higher in March. Komar fishing was done only in March and April and its CPUE was greater in both the months than that of kochal. The average recovery rate (combination of all six species) was 37.80 considering the stocking from July month of the previous year. The recovery rate of common carp (54.1) was the highest and lowest (13.90) in case of silver carp. When the recovery was calculated on the basis of one year data and stocking, it was 55.6%. Analysis of production model revealed that the present production (54,806 kg/year) is less than both theoretical production (model I- 85,285 kg/year and model II -75,952 kg/year) estimated. Therefore, it may be concluded that the fish production from Nasti baor could still be increased from the present level of production.

Effects of different levels of energy and protein sources on the growth performance, feeding, survival rate and the chemical body composition of juvenile Pacific white shrimp (Litopenaeus vannamei)

The present study was carried out in order to establish an economical effective diet for the pacific white shrimp in the southern part conditions of Iran. With the consideration of three dietary energy levels (E1=262, E2=312, E3=362 kcal 100 g-1 diet) and six ratios of fish meal (FM) to soybean meal (SBM) [(P1=100%FM+0%SBM, P2=80%FM+20%SBM, P3=60%FM+40%SBM, P4=40%FM+60%SBM, P5=20%FM+80%SBM, P6=0%FM+100%SBM)], 18 experimental diets (with 36% crude protein) were prepared. Completely randomized design was used to assign 54 polyethylene 300 litre round tanks provided by aeration and flow through water system and was stocked by 19 juvenile as 3 replicates to each treatment. Shrimps average weight was about 0.77 grams at the start. After 56 days culture period, maximum growth and nutritional performances were observed in the P6E1 treatment (containing 100% soybean meal and 262 kcal 100 g-1 diet) and P5E1 treatment (containing 80% soybean meal and 262 kcal 100 g-1 diet). Also the highest survival rate of the shrimps was observed in the P1E1, P1E2, P3E3 and P5E3 treatments. Additionally interactive effect of different protein ratios and energy levels had significant difference on body protein, fat, fiber and ash contents (P<0.05). Results of the present study suggest the possibility replacement of at least 80% of dietary fish meal by soybean meal in the diet of pacific white shrimp in the conditions of southern part of Iran.

Genetic diversity of Saccostrea cucullata population in the northern coast in Persian Gulf and Oman Sea

Genetic diversity of Saccostrea cucullata in the northern coast lines of the Persian Gulf and the Sea of Oman were determined using DNA extraction and RAPD - PCR. A total of 300 samples were collected from 6 station along the coastline. Two out of six primers showed positive results namely GCG - ATC - CCC - A (Primer 1) and GTC - CAC - ACG - C (Primer 5) which were in accordance with morphometric analysis. The number of bands in the two above - mentioned primers in Khor - Tang and Chabahar station (Province of Sistan and Balouchestan) was significantly different from the number of produced bands in Dayer and Bushehr station (Province of Bushehr) as well as Gheshm and Bandar - Lengeh station (Province of Hormozgan). The cluster analisys was used to confirm the above variations. The results showed that the oyster population can be divided into two separate clusters. The first cluster included Bushehr Dayer Gheshm and Bandar - Lengeh species. The second cluster included Khor - Tang and Chabahar species. The analysis also showed that the first cluster can be divided into two Sub — cluster. Bushehr and Dayer belong to one Sub - cluster whereas Gheshm and Bandar - Lengeh form the other Sub — cluster. The formation of different vluster can be related to Physico - Chemical properties of water and climatic variations in different habitats along the Persion Gulf and the Sea of Oman. Key words: Molecular genetics, Population, RAND, PCR' Saccostrea cucullata