Identification of formalin-preserved eggs of red sea bream (Pagrus major) (Pisces: Sparidae) using monoclonal antibodies

Catches of important commercial fish such as red sea bream, flat fish, and yellowtail are decreasing in Japan. In order to sustain these species it is especially important that their distribution and biomass at all life stages are known. However, information on the early life stages of these species is limited because identifying the eggs and larvae of such fish is sometimes extremely difficult.

A comparative study on the hatching of common carp eggs in hapa and hatchery (model CIFE D-80)

Common carp (Cyprinus carpio) eggs were incubated to study the efficiency of hatching in hapa and hatchery. During incubation the recorded temperature was 21-28 degree C and 20-31 degree C, dissolved oxygen 6-9 ppm. and 3-5 ppm., total alkalinity 180-250 ppm. and 28-62 ppm. respectively in the hatchery (model C.I.F.E. D-80) and hapa. CO sub(2) was totally absent in the hatchery, but recorded 3-10 ppm. in the hapa. The flow of water was maintained at 1.25 l/minute/jar in the hatchery. Under the above environmental conditions the eggs hatched in 42-51 hrs. in the hatchery and 61-81 hrs. in the hapa from egg to spawn thereby establishing the hatchery to be a better hatching system for carp eggs.

Variability in the distribution of planktonic fish eggs and larvae in the nearshore waters off Bombay

Distribution of planktonic fish eggs and larvae in the nearshore waters off Bombay was studied during November 1979 to December 1980. Monthly samples were collected along three transects (Versova, Mahim and Thana) covering eleven stations which represented different environmental conditions. Fish eggs and larvae were common in the area of study with maximum abundance in December 1979 and April/August 1980. Mean density of fish eggs was maximum along the Mahim transect while population of larvae was more in the Versova transect. Total number of families of fish larvae represented in the collections increased from nearshore to offshore area.

Laboratory reared eggs and larval stages of the clupeid fish, Opisthopterus tardoore (Cuvier)

The early embryonic and larval stages of Opisthopterus tardoore are described and discussed. The developing 3 eggs of stages up to 21hrs after collection and larvae up to 118hrs after hatching were reared in the laboratory. Seasonal occurrence of the eggs in the Vellar estuary, Porto Novo (lat. 11 29'N; 79 degree 46'E) was recorded for a period of 2 years (Nov. 1977 to Oct. 1979). Variation in myotomic counts in the larvae is critically reviewed with earlier works.

Seasonal fluctuation in the distribution of eggs and larvae of flat fishes (Pleuronectiformes - Pisces) in the Cochin backwater

Attempts have been made to correlate the abundance of eggs and larvae of flat fishes belonging to families Cynoglossidae and Soleidae with the hydrographical parameters of the environment, particularly the salinity. The eggs and larvae were found to occur in the Cochin backwater during the pre-monsoon period but not during the south west monsoon period. During the south west monsoon period larvae belonging to very early stages were found at the marine end (Fairway Buoy). The continued or prolonged existence of very low salinity appears to restrict the distribution of flat fish larvae. In the post-monsoon period also the eggs and larvae were not found in regions of very low salinity. Larvae of Cynoglossus puncticeps (Richardson), C. brevis Gunther, C. cynoglossus (Ham. Buch), C. lida (Bleeker) and Solea heinii Steindachner were obtained in the collection taken from the estuary. From the data it is seen that each species has specific spawning period.

Effect of combined thyroxine and cortisol treatment on hatching of eggs, post-embryonic growth and survival of larvae of Heteropneustes fossilis

In order to record the effects of thyroxine and cortisol (individual/combined) on hatching, post-embryonic growth and survival of larvae of Heteropneustes fossilis, newly fertilized eggs were given bath immersion treatments of L-thyroxine (T sub(4); 0.05 mg/l), cortisol (0.50 mg/l) and T sub(4)+ cortisol (0.05 mg/l+0.50 mg/l) for 15 days. Hatching of eggs, growth and survival of the larvae improved significantly (P<0.001) in the hormone treated groups as compared to those of control. The frequency of deformities was reduced in the combined hormone treatment group. The present observations suggest that the advanced digestive function probably induced by T sub(4)+cortisol treatment might have resulted in improvement in food utilization during the critical phases of first feeding and promoted vital developmental processes resulting in uniform growth, decreased mortality, better survival and transformation of larvae to juveniles. This combined hormone therapy appears to have practical utility in fish hatchery practice for better success in larval rearing.

A new observation on large scale disintegration of eggs of Indian major carps during development

While carp themselves may destroy carp eggs by sucking from outside the happa, destruction in the absence of carp also occurs. During observations of the effect of electrical field on developing carp eggs it was seen that the egg membrane ruptured prematurely under certain potential differences. In natural waters and mud potential differences occur in the presence of high concentrations of certain salts compared with the internal concentration. Temperature and pH fluctuations following strong sun or heavy rain accelerates the diffusion of ions along side water through the egg membrane. The egg cells expand rapidly and the embryos were rapidly dropped.

Cholesterol content in the eggs of some freshwater teleosts

The authors report the distribution of cholesterol in the ripe, unspawned eggs of 10 spp of teleost (carps, catfish and murrels) together with values for egg diam. The egg, with its relatively abundant yolk, is shown to be fairly rich in cholesterol, though values differ from sp to sp. The lowest value (700 mg/100 g) was recorded from Wallago attu, and the highest (1537 mg/100g) from Heteropneustes fossilis. In general the levels are lowest in carp eggs. No definite relationship could be established between lipid and cholesterol contents, or between egg diam and total cholesterol. The role of cholesterol in physiology of fish is discussed; it is noted that the carnivorous fish tended to have higher levels of cholesterol in their eggs.

Studies on the toxicological effects of Asulox-40 and Emisan-6 to eggs and early life history stages of Sarotherodon mossambicus

Toxicological effects of Asulox-40 and Emisan-6 to eggs and early life history stages of Sarotherodon mossambicus were reported. 80% of egg hatching occurred in the controls, 1 p.p.m and 5 p.p.m concentrations of Asulox-40. 10 p.p.m. and 50 p.p.m. concentrations of the same toxicants had 70% and 60% hatchings while in Emisan-6 in the same concentrations the hatching were 70% and! 40%. In 100 p.p.m. concentration of both toxicants 20% incomplete hatching occurred. In Emisan-6 Lc 50 and Lc 100 values were recorded at 32 hand 96h respectively in 10 p.p.m. concentrations. In Asulox-40 the same values were recorded in 24h and 40h respectively at 50 p.p.m. concentration. The fish activity during the experimental period showed initial hyper activity. It was established that the Emisan-6 is more harmful to S. mossambicus than Asulox-40. The harmless concentrations of these chemicals were 1.2 p.p.m. for Asulox-40 and 0.6 p.p.m. for Emisan-6.

Viability of Penaeus monodon eggs after simulated transport conditions

P. monodon spawners, transported from maturation pens suffer from stress which in turn may lead to lowered spawning rate or fertility. Spawning the females in the maturation site and transporting the eggs to the hatchery site is being considered as an alternative. Egg transport costs may be reduced to a minimum by using eggs from ablated spawners, transported at high density with no aeration. Experiments on higher egg densities as well as on transport of nauplii should, however, be undertaken.

Observations on the artificial fertilization of eggs and larval rearing of the grey mullet, Mugil cephalus L.

The results are presented of attempts to artificially fertilize Mugil cephalus eggs in the Philippines. Embryonic development is outlined and rearing of the larvae described. Mass mortality occurred during week 3 of rearing.

The use of decapsulated brine shrimp eggs as food for shrimp larvae

Decapsulated cysts of Artemia salina were experimentally fed to the larvae of Penaeus monodon, Metapenaeus ensis, M. endeavouri and Macrobrachium rosenbergii and were found to be comparable to freshly hatched brine shrimp nauplii.

In-vitro effect of fungicides on hyphal growth and sporogenesis of Lagenidium sp. isolated from Penaeus monodon larvae and Scylla serrata eggs

The sensitivity of Lagenidium, isolated from Penaeus monodon, Scylla serrata , to 34 antimycotics was determined. Effects on the development of vesicles, zoospores and mycelial growth were evaluated. Although mycoidal levels of the chemicals tested will be ideal for lethal treatment on control of the fungus, the high dose required may be lethal to the host, thus the use of mycostatic concentrations is more practical. Treatments of rearing water containing larvae, adult shrimps or crabs should be done only after preliminary tolerance experiments using at least the mycostatic dose prove to be safe for the hosts. Mycocidal doses can be used for determining disinfection doses of equipment and facilities used in rearing procedures as well as for destroying batches of infected larvae.

Occurrence of milkfish, Chanos chanos (Forsskal) eggs around Panay Island, Philippines

The study documents the occurrence of milkfish, Chanos chanos (Forsskal) eggs around Panay Island, Philippines: in Cuyo East Pass, Panay Gulf, Visayan Sea, Sibuyan Sea and Cagayan Islands. Most of the eggs, 545 out of 551, were obtained from Cuyo East Pass compared to Cagayan Islands and Sibuyan Sea while no milkfish egg was recovered from either Panay Gulf or Visayan Sea.

Results of drift card experiments and considerations on the movement of milkfish eggs and larvae in the northern Sulu Sea

For a period of one year beginning December 1977, drift card experiments were conducted off the western and southern coasts of Panay Island to determine the surface currents in the area. Of a total 2,384 drift cards released during the study, 382 (16.02%) were recovered, 92% of them within 30 days following dispatch. The surface currents in the study area are strongly influenced, in direction and speed, by the prevailing monsoon winds. During the NE monsoon period, the surface currents move away from the coast; during the SW monsoon, toward and/or parallel to the coast. Based on the results, the probable movement and transport of milkfish (Chanos chanos) eggs and larvae from the spawning ground to the fry collection ground are also discussed.