An investigation of survey technologies and modeling techniques for improving deepwater surveys of nonindigenous species in the Northwestern Hawaiian Islands

Nonindigenous species (NIS) are a major threat to marine ecosystems, with possible dramatic effects on biodiversity, biological productivity, habitat structure and fisheries. The Papahānaumokuākea Marine National Monument (PMNM) has taken active steps to mitigate the threats of NIS in Northwestern Hawaiian Islands (NWHI). Of particular concern are the 13 NIS already detected in NWHI and two invasive species found among the main Hawaiian Islands, snowflake coral (Carijoa riseii) and a red alga (Hypnea musciformis). Much of the information regarding NIS in NWHI has been collected or informed by surveys using conventional SCUBA or fishing gear. These technologies have significant drawbacks. SCUBA is generally constrained to depths shallower than 40 m and several NIS of concern have been detected well below this limit (e.g., L. kasmira – 256 m) and fishing gear is highly selective. Consequently, not all habitats or species can be properly represented. Effective management of NIS requires knowledge of their spatial distribution and abundance over their entire range. Surveys which provide this requisite information can be expensive, especially in the marine environment and even more so in deepwater. Technologies which minimize costs, increase the probability of detection and are capable of satisfying multiple objectives simultaneously are desired. This report examines survey technologies, with a focus on towed camera systems (TCSs), and modeling techniques which can increase NIS detection and sampling efficiency in deepwater habitats of NWHI; thus filling a critical data gap in present datasets. A pilot study conducted in 2008 at French Frigate Shoals and Brooks Banks was used to investigate the application of TCSs for surveying NIS in habitats deeper than 40 m. Cost and data quality were assessed. Over 100 hours of video was collected, in which 124 sightings of NIS were made among benthic habitats from 20 to 250 m. Most sightings were of a single cosmopolitan species, Lutjanus kasmira, but Cephalopholis argus, and Lutjanus fulvus, were also detected. The data expand the spatial distributions of observed NIS into deepwater habitats, identify algal plain as an important habitat and complement existing data collected using SCUBA and fishing gear. The technology’s principal drawback was its inability to identify organisms of particular concern, such as Carijoa riseii and Hypnea musciformis due to inadequate camera resolution and inability to thoroughly inspect sites. To solve this issue we recommend incorporating high-resolution cameras into TCSs, or using alternative technologies, such as technical SCUBA diving or remotely operated vehicles, in place of TCSs. We compared several different survey technologies by cost and their ability to detect NIS and these results are summarized in Table 3.

Histological techniques for marine bivalve molluscs: update

This chapter describes the procedures for determining the reproductive stage of oysters, mytilid mussels, and dreissenid mussels collected for NOAA’s National Status and Trends Mussel Watch Project. Analyses are conducted on paraffin-embedded tissues sectioned at a 5-μm thickness and stained using a pentachrome staining procedure. Each slide is examined microscopically to determine the animal’s sex and stage of gonadal development. A semi-quantitative ranking is assigned.

Histological techniques for marine bivalve mollusks and crustaceans, 2nd edition

Investigators at the Cooperative Oxford Laboratory (COL) diagnose and study crustaceans, mollusks, finfish, and a variety of other marine and estuarine invertebrates to assess animal health. This edition updates the Histological Techniques for Marine Bivalve Mollusks manual by Howard and Smith (1983) with additional chapters on molluscan and crustacean techniques. The new edition is intended to serve as a guide for histological processing of shellfish, principally bivalve mollusks and crustaceans. Basically, the techniques included are applicable for histopathological preparation of all marine animals, recognizing however that initial necropsy is unique to each species. Photographs and illustrations are provided for instruction on necropsy of different species to simplify the processing of tissues. Several of the procedures described are adaptations developed by the COL staff. They represent techniques based on principles established for the histopathologic study of mammalian and other vertebrate tissues, but modified for marine and aquatic invertebrates. Although the manual attempts to provide adequate information on techniques, it is also intended to serve as a useful reference source to those interested in the pathology of marine animals. General references and recommended reading listed in the back of the manual will provide histological information on species not addressed in the text.

Atmospheric chlorofluoromethanes as tracers of ocean circulation and mixing: measurement and calibration techniques and studies in the Greenland and Norwegian seas

EXTRACT (SEE PDF FOR FULL ABSTRACT): An analytical system was designed and constructed for the rapid and accurate shipboard measurement of anthropogenic chlorofluoromethanes in seawater and in air, using electron capture gas chrometography. The distribution of these compounds in the marine atmosphere and the water column in the Greenland and Norwegian seas were studied during February and March, 1982. The compounds, dissolved in the ocean from the atmosphere, can be used as tracers of subsurface ocean circulation and mixing processes.

A modern pollen record from the central Gulf of California

As one facet of an effort to tie the pollen record of central Gulf of California deep cores to modern analogs, pollen was analyzed in the uppermost 150-200 years of varved core 7807-1410 taken nearby. Sampling at 2- to 8-year resolution yielded a noncomplacent record, suggesting pollen in these sediments may be a potential high resolution proxy record of short-term climatic events. The pollen spectrum as a whole matches that of upper-most DSDP Site 480 (means of all samples). Lack of a ratio or influx shift following damming of local rivers and a surplus of low-spine Compositae pollen relative to mainland sites support Baumgartner's theory that terrigenous influx to the site is largely aeolian and also suggest that a significant fraction of the pollen influx may come from Baja California.

Progress of modern aquaculture

The development of the aquaculture industry worldwide is discussed in detail with respect to activities conducted by various international organizations, considering in particular the leading role played by the FAO and its regional agencies. A brief examination is also made of the current situation regarding the industry in India and the potential for further development and improvement.

Mechanization of fishing craft and the use of improved fishing gear

Since the year 1925, attention has been focused periodically on the stagnation in the local fishing industry, and those who have studied the subject have been unanimous about the need to introduce modern fishing craft which are capable of working more fishing gear. This report outlines the stages through which the evolution of more effective fishing operations has progressed, both in the gradually increasing use of mechanical propulsion for boats and in the adoption of more modern gear and techniques by local fishermen. No reference is made in this report to the operation of deep-sea trawlers (Sivalingam, 1956) a phase of development which has hitherto not influenced the local fishing industry to any appreciable extent.

Manipulation of chromosomes in fish: review of various techniques and their implications in aquaculture

Human ingenuity has made it possible to advent the chromosome manipulation techniques to produce individuals with differing genomic status in a number of fish using various causal agents such as physical shocks (temperature or hydrostatic pressure), chemical (endomitotics) and anesthetic treatments either to suppress the second meiotic division shortly after fertilization of eggs or to prevent the first mitotic division shortly prior to mitotic cleavage formation. This results in the induction of polyploidy (triploidy and tetraploidy), gynogenesis (both meiotic and mitotic leading to clonal lines) and androgenesis in fish population. The rationale for the induction of such ploidy in fish has been its potential for generating sterile individuals, rapidly inbred lines and masculinized fish, which could be of benefit to fish farming and aquaculture. In this paper, these are critically reviewed and the implication of recently developed chromosome manipulation techniques to various fin fishes is discussed.

Techniques for the transport and maintenance of scleractinian coral

Several small scleractinian coral colonies were collected from a remote reef and transferred [to] the Louisiana Universities Marine Center (LUMCON) for in vitro reproductive and larval studies. The species used here were Porites astreoides and Diploria strigosa. Colony size was ~20 cm in diameter. Colonies were brought to the surface by liftbag and stored in modified ice coolers. They were transported from Freeport, TX to Cocodrie, LA by truck for nearly 15 hours where field conditions were simulated in waiting aquaria. This document describes the techniques and equipment that were used, how to outfit such aquaria, proper handling techniques for coral colonies, and several eventualities that the mariculturist should be prepared for in undertaking this endeavor. It will hopefully prevent many mistakes from being made.

Production of triploid African catfish, Clarias gariepinus (Burchell), using chromosome manipulation techniques

An experiment was conducted to induce triploidy in African catfish, Clarias gariepinus, using heat shock and cold shock techniques. Cold shock at a temperature of 0± 1°C and 5±1°C for a duration of 15, 30, 45 and 60 min and heat shock at a temperature of 40±0.5°C and 41 ±OS C for a duration of 1, 2 and 3 min was given to induce triploidy 5 min after fertilization. Maximum percentage of triploids (91.4%) were obtained in the heat shock at a temperature of 40±0SC for a duration of 1 min whereas cold shock at 0± 1 C for a duration of 60 min yielded 90% of triploids. Chromosome analysis revealed that diploids have 54 chromosomes and triploids have 81 chromosomes. The erythrocyte measurements of the minor axis and major axis were 1.17 times larger in treated fish than in controls. The growth studies showed that the growth rate was not significantly affected in triploids.