Seasonal and spatial distribution of Chlorophyll a in the north Arabian Sea bordering Pakistan

The average integrated chlorophyll a values for a 30-m deep surface layer in the north Arabian Sea bordering Pakistan ranged from negligible amounts to as high as 0.53μg chl. a 1ˉ¹(15.9mg mˉ²) during the period January 20, 1977 to June 4, 1977. The values, in general, decreased offshore except for the westernmost part of the Makran shelf, where unexpectedly high values were recorded over deep water. Seasonal distribution showed very high values in January (northeast monsoon season) which, with a few exceptions, gradually decreased to very low values in May, and then increased in June. The January peak may be related to winter cooling of surface waters resulting in convection and the June peak to the onset of southwest monsoon season in May. Coastal water shallower than 30m showed no seasonality and were often sites of intense phytoplankton blooms.

Evaluation of chemical tests for the quality of prawns

The object of this study was to determine the value of physical, bacteriological and chemical tests used to find out and compare the indices of quality of prawns stored at 0°C and at 18°C. pH value, nature of drip, the total bacterial count, presence or absence of tryptophan, trimethylamine content, glycogen, lactic acid, vitamins such as thiamin, riboflavin and niacin were estimated periodically to evaluate the quality of prawns stored at 0°C, whereas in addition to organoleptic changes, pH, bacterial count, nature of growth in peptone water, soluble protein in salt solution and loss of moisture, glycogen, lactic acid, and changes in vitamin B contents were noted periodically for prawns stored at -l8°C. Riboflavin and niacin were not affected appreciably but the retention of thiamin in prawns was very low.

On the production and nutritive value of fish meal from Silver belly (Leiognathus spp.) landings at Rameswaram

Silver belly (Leiognathus Spp.) forms a major fishery in recent years in the Rameswaram island but fetches for the fishermen very low prices ranging from Rs. 0.03 to 0.12/Kg only, there being practically no demand for the fish. The possibilities of utilizing this cheap fish are discussed and the processing method described. During the glut season the cost of production of Silver belly fish meal works out to competitive prices of Rs. 500 to 700/ton. The silver belly fish meal is of high quality with good protein content averaging 57.71% in commercial samples and 61.90% in laboratory samples and with a high pepsin digestibility of 90.0% to 92.5%. The essential amino acid composition of the Silver belly fish meal compares very favorably with other round fish meals, with high contents of lysine, leucine, arginine, isoleucine, methionine, phenyl alanine, threonine and valine. Since there is good demand for fish meal as poultry and cattle food both in the internal and external markets, there is good scope for large scale production and sale of fish meal.

Quantitative and qualitative studies on the bacterial flora of fresh sardines

Plate counts at R T and 8 C on the skin with muscle and the gut contents of absolutely fresh sardines (Sardinella longiceps) caught off Cochin showed a seasonal variation when sampling was done over a period of 12 months. The counts of the gut contents ran parallel with those of the skin with muscle, but at a higher level of magnitude. Qualitatively, the analysis of 360 strains of bacteria isolated from the skin with muscle and 100 strains from the guts during a year's study revealed a very high preponderance of Gram negative rods, mainly of Achromobacter, Vibrio, and Pseudomonas groups. The percentage of Gram positive organism was very low or nil at times in the ocean fresh sardines.

Seasonal fluctuation in the distribution of eggs and larvae of flat fishes (Pleuronectiformes - Pisces) in the Cochin backwater

Attempts have been made to correlate the abundance of eggs and larvae of flat fishes belonging to families Cynoglossidae and Soleidae with the hydrographical parameters of the environment, particularly the salinity. The eggs and larvae were found to occur in the Cochin backwater during the pre-monsoon period but not during the south west monsoon period. During the south west monsoon period larvae belonging to very early stages were found at the marine end (Fairway Buoy). The continued or prolonged existence of very low salinity appears to restrict the distribution of flat fish larvae. In the post-monsoon period also the eggs and larvae were not found in regions of very low salinity. Larvae of Cynoglossus puncticeps (Richardson), C. brevis Gunther, C. cynoglossus (Ham. Buch), C. lida (Bleeker) and Solea heinii Steindachner were obtained in the collection taken from the estuary. From the data it is seen that each species has specific spawning period.

The 66 k-Da protein identified as a light meromyosin is involved in the setting of surimi

The 66 kilo-Dalton (k-Da) protein split off from the cross linked myosin heavy chain (CMHC) formed due to the setting of Alaska pollack surimi, frozen-storage of Pacific cod flesh, and vinegar-curing of Pacific mackerel mince was identified as a light meromyosin (LMM). Puncture and stress-relaxation tests showed that the actomyosin subunits (AMS) of Alaska pollack surimi, upon setting at 30°C, transformed into gel, although the elasticity of this gel was very low when compared to the gels from surimi or actomyosin (AM). Electrophoretic studies showed that the band due to LMM in the gel from AMS gradually disappeared with the progress of setting but higher molecular weight polymer did not form. The intensity of the bands due to other myosin sub-fragments decreased a little. The findings suggest that at setting temperature, LMM of MHC molecule leads to an unfolding resulting in an intramolecular aggregation through non-covalent interactions, and thus plays a significant role in the crosslinking of MHC.

Effect of decapsulation on viability and hatching performance of Artemia cysts at different salinity levels

Artemia cysts were produced from the traditional solar salt works of Bangladesh through different fertilization treatments were tested for viability and hatching performance in different forms, such as processed and preserved, processed and decapsulated and unprocessed and undecapsulated. Decapsulated cysts performed maximum hatching (86.0%) in 20ppt salinity during 48 hours of incubation. The hatching percentage by the unprocessed and undecapsulated cysts were very low (12.0- 18.7%) in all the tested salinity grades.

Effects of culture treatments on HUFA levels in Artemia cysts

Artemia cysts (of GSL, Utah, USA origin) were produced from the modified traditional solar salt works of Bangladesh during winter months through different feeding/fertilization treatments (T1, T2, T3, T4 ) were analyzed to understand the effects of treatments on their fatty acid profile. Palmitic, Linolenic, Eicosapantaenonic and Docohexaenoic acids (mg/g. DW) were found highest for the cysts in T1 (16.0% ±1 .36), T2 (14.7% ±0.47), T3 (4.7% ±0.40) and T4 (0.7% ±0.06) treatments, respectively. High amount of 18:3(n-3) acids in the cysts of all sources proves to be freshwater type of the cysts. The presence of marine type essential fatty acids in the cysts of all sources were found low for 20:5n-3 (3.7-4.7%) and very low for 22:6n-3 (0.09-0.7%). No significant variation was observed for 16:0 acids within the treatments, but for 18:3(n-3) acid, the variation was found highly significant (P= 0.0052) between T2 and T4 treatments. For 20:5(n-3), only variation between T2 and T4 was found insignificant (P=0.1161), but between other treatments, significant variation was observed between T2 and T4 (P=0.0241), T2 and T4 (P=0.0022) and T1 andT4 (P=0.0161). No significant variation was found in other treatments.

Physiological observations on a diatom Skeletonema costatum (Greville) Cleve

A chain-forming diatom Skeletonema costatum (Greville) Cleve collected from Yatsushiro Sea, Japan was cultured to determine the optimum level of some physico-chemical factors for their growth under laboratory conditions. Filtered and sterilized aged sea water enriched by adding nutrient solution (Provasoli 1968) was used as the culture medium. The plankton could tolerate a wide range of salinities (3-55 ppt). Optimum growth was observed at salinities of 20-35 ppt, temperatures of 20-25°C, light intensities of 80-120µE mˉ² secˉ¹ and pH between 7.5 and 8.0. Growth did not occur at salinities below 3 ppt and at temperatures above 30°C. From the present study, it is concluded that S. costatum was extremely euryhaline and tolerable to very low salinities.

Elevating fishers of Dal Lake in Jammu and Kashmir

The deteriorating condition of one of the most magnificent lakes of India, the Dal Lake, has provoked a lot of research interest from the biological and ecological points of view. However, the impact of deterioration of the ecology on the inhabitant fisher folk around Dal Lake has not been given much importance. In this study, the three major beats of the lake, namely, Hazratbal, Nishat and Nehru Park, were surveyed, randomly taking 10% of the fishermen families. It was observed that the summer mean catch per day (5.18 ± 0.69 kg) was more than the mean winter catch per day (2.85 ± 0.40 kg). However, the total annual catch per person was only 1195.56 ± 211.63 kg. Moreover, marketing through middlemen, especially, during summer, was observed to be very low fetching in terms of mean price per kilogram fish, amounting to Rs 37.50 ± 2.54 for local fish and Rs 22.50 ± 2.88 for the carp. It was also observed that the fisher folk had borrowed a good amount of money for the repair and construction of their houses and boats. Therefore, on the whole, the fishing business around Dal Lake was observed to be under severe stress. In this context, this paper has come out with recommendations to uplift the socio-economic conditions of the fishers.

New evidences on the distribution of predatory pelagic sharks in the tuna grounds of the Indian Ocean

Since the commencement of the exploitation of oceanic tuna resources of the Indian Ocean seventeen years ago, the hooked rates for the tuna species have declined in many areas of the Ocean but there are no evidences of such a trend in the case of the sharks. As a result, the percentage composition of sharks in the longline catches and the percentage of the tuna catch damaged by sharks show an increase. Hence there is an urgent need for innovation of the existing longline gear in order to increase the fishing efficiency for hooking the tuna species with a corresponding reduction in its efficiency for hooking sharks. At the beginning of this fishery, hooked sharks were discarded at sea, at a later stage the liver and fins were taken and the carcass discarded and presently the sharks are also brought along with the tuna catch. Though the shark meat has a very low market value it is brought in order to cover up for the declining tuna catches. Thus it has become very necessary to increase the demand for shark meat by developing products or by-products utilizing shark meat and ensuring the successful continuity of the tuna longline fishery. The pattern of distribution of shark species in the time grounds of the Pacific, Indian and Atlantic Oceans and also the predation of hooked tunas by sharks were discussed earlier (Sivasubranianiam 1963, 1964 and 1966). Some contribution to these studies is made in this paper based on new data become available.

The history and fishery potential of Kisoro Minor Lakes part two: Preliminary survey of Lakes Mutanda and Mulehe fisheries 

The fisheries of Lakes Mutanda and Mulehe during 1998/9 were mainly at subsistence scale and only few fishers operated at irregular intervals. The commercial catch records between 1963 to 1999 showed that Lake Mulehe was landing more fish than Lake Mutanda despite the fact that Lake Mutanda (26.4 km2) was bigger than Lake Mulehe (4.11 cm2). The constant decline of catches was due to irregular restocking and applying low stocking densities of fry. However, restocking should consider using species that withstand low temperature (15-240C) in the district. These include Oreochromis niloticus (Nile tilapia), Macropterus salmoides (Black bass), and Cyprinus carpio (Common carp). Most of these species have either disappeared or declined to very low levels. Due to lack of commercial fish species for harvest, the fishers by 1998/9 resorted to harvesting the haplochromines, Clarias carsoni and edible frogs (Xenopus kigesiensis) as alternative resources. Experimental studies have shown the need and techniques to enhance fish production on these two lakes.

Growth performance, recovery rate and fish yield of GIFT strain at various water depths under rice-fish culture systems

Effect of water depth on recovery rate, growth performance and fish yield of GIFT in the rice-fish production systems was studies in experimental plots of 123 m2 with a pond refuge of I meter deep which covered 10% of the total land area. Mortality rate of fish was very low ranging from 0.81-1.63%. However, at harvest, recovery rate ranged from 76.69-82.93% with the highest recovery at 11-15 em of water depth. Significantly the highest absolute growth (99.97) and specific growth rate (2.42%) were found at 21-25 cm water depth. The same treatment also produced significantly higher fish yield (909.76 kg/ha) although statistically similar to the fish yield (862.60 kg/ha) obtained at ll-15 em of water depth. Results also suggested that higher water depth can produce bigger fish but no significant effects of water depth was found on fish yield in the treatments 11-15 cm and 21-25 cm water depths of this experiment.

Seasonal fluctuation in primary production in relation to the physicochemical parameters of two weed-infested ponds of Kalyani, West Bengal

Kalyani lake (P sub(1)), a weed infested recreational water body and a weed chocked derelict water body (P sub(2)) in the heart of Kalyani city of West Bengal were studied for a period of one year for their primary productivity and other physicochemical parameters. Very low primary productivity (GPP=360-1237mg C m super(-2) d super(-1); NPP=157-787 mg C m super(-2) d super(-1)) was recorded in P sub(2) in spite of having a high concentration of nutrients (PO sub(4)–P=0.052-0.260mg l super(- 1); NO sub(3)-N=0.110-0.412mg l super(-1)). On the other hand, moderate primary productivity (GPP=1687-3195mg C m super(-2) d super(-1); NPP=900-2700mg C m super(-2) d super(-1)) was found in P sub(1) with comparatively low range of nutrients (P0 sub(4)-P =0.010-0.058mg l super(-1); NO sub(3)-N=0.032-0.118mg l super(-1)). After studying the other physicochemical parameters (temperature, transparency, dissolved oxygen, free carbon dioxide, pH, alkalinity and macrophytic biomass), it was found that the overall hydro-biological conditions of the weed-chocked derelict water body (P sub(2)) is not congenial for biological production as compared to Kalyani Lake (P sub(1)). Kalyani Lake may be used for fish culture with proper management practices.

Estimation of micro-flora associated with different stages of Macrobrachium rosenbergii (de Man)

The bacterial flora occurring in muscle, haemolymph, hepatopancreas and gill of brood, juveniles, water, eggs, larvae and rearing water were estimated by selective plate count technique for Entrobacteriaceae, Streptococaceae and Vibrionaceae members. The total viable bacterial count was estimated by total plate count technique on nutrient agar. The total viable counts of bacteria were lowest in water from 6.10x10² CFU/mL) and highest in egg (6.06x10super(8) CFU/g). In brood the total counts were varying from 1.62x10² CFU/g in muscle to 2.20x10super(5) CFU/g in gills. In juveniles, the total plate counts were varying from 2.8x10super(4) CFU/g in muscles to 3.67x10 super(8) CFU/g in hepatopancreas. Selective plate counts show that Enterobacteriaceae members dominate in egg and gills of brood and hepatopancreas of juveniles. Vibrios were found to be dominant in water and larvae of rearing tank. Haemolymph of brood was sterile and did not contain any bacteria while muscle of juvenile was having very low count of total viable bacteria.