Technological aspects of preservation and processing of edible shell fishes V. Cold storage changes in mussels (Mytilus edulis) and clams (Villortia [i.e. Villorita] sp.)

The changes in chemical, bacteriological and organoleptic qualities of mussels and clams during freezing and subsequent frozen storage have been studied in relation to the holding time in ice prior to freezing and the shelf-life of the product is determined.

A review on oil sardine 1. Distribution, preservation and transportation

Oil sardine (Sardinella longiceps) is widely reported from the Indian Ocean and southeast Asia coasts. It is found, with other less important spp of Sardinella, around both coasts of India. Landings have shown wide variations from yr to yr. Figures were 7412 tons in 1956 and 301,641 tons in 1968. Various possible reasons for this are noted. The main fishery is concentrated in coastal waters 12-15 km from shore in waters up to 15 m deep. The gears used are mostly seine nets. Though the fish has a good protein value, its prices do not compare well to other fish, often due to handling and preservation difficulties. Problems encountered during preservation and transportation of the fish are considered. These include bursting and rancidity.

A review on oil sardine 2. Preservation by canning, curing and smoking

Commercial canning of oil sardine (Sardinella longiceps) in India is a relatively new procedure. Although 7 firms are engaged in canning this compares poorly with the abundance of the fish. There are often wide variations in the quality of the canned fish and important chemical and physical variations occur in the product once canned. A description of the canning process is given, and production figures compared to those of other countries. Production figures for 1965 to 1969 are given. These show that production increased from 1.2 to 1.5 million cans, but that there was a peak in 1967 when 3.2 million can s were produced. Exports of canned marine fish by country, and production of caned sardine by country from 1965 to 1970 are tabulated. The types of containers used and the feasibility of exporting canned fish are considered. Finally, the preparation of cured and smoked products is discussed briefly.

Design of refrigerated sea water plant for preservation of fish

To study the feasibility of employing refrigerated sea water on board fishing vessels for the preservation of fish, a pilot model has been designed, the details of which are presented in this paper.

Studies on preservation of fish in refrigerated sea-water

Storage characteristics of oil sardine (Sardinella longiceps), mackerel (Rastrelliger kanagurta) and seer (Scomberomorus guttatus) in refrigerated sea-water (RSW) were studied in comparison with their storage in crushed ice. Oil sardine stored in RSW was found to be comparable to iced ones only during the initial stages (up to 2 days) of storage and on further storage the former was found to be inferior to the latter. RSW can be advantageously employed for preservation of mackerel and seer. Mackerel and seer could be stored in RSW in acceptable condition for 4 to 6 days and 12 to 14 days respectively.

Commercial studies on radiation preservation of fish and shell-fish in Canada

The author describes work being undertaken at the Fisheries Research Board of Canada's Laboratory at Halifax (Nova Scotia). These studies relate particularly to the irradiation of fish and shellfish for storage, and bacterial analysis of irradiated products.

Preservation of Sardinella longiceps in iced and chilled seawater. Part 1 - Changes during storage with particular reference to bacterial load and nitrogenous compounds

Oil sardines in prime condition were subjected to onboard chilling. Two lots were chilled in CSW (samples C and CI), a third lot was chilled in crushed ice (sample I) and a fourth lot left not iced on deck (Sample AI). Upon landing sample AI was iced and sample CI was removed from the CSW and iced. All the four samples were kept in a chilled room for storage studies. The fish chilled and stored in CSW recorded the least, and the fish subjected to delayed icing, the highest values for all the indices of spoilage namely, free amino nitrogen, trimethylamine (TMA) and total volatile base nitrogen (TVBN). The total psychrophilic bacterial number also showed a similar trend. The organoleptic assessment of the cooked samples revealed C I, CI, AI to be the order of preference throughout the storage. This assessment was found to hold good for the rest of the parameters as well. The CSW held fishes were found to be distinctly superior to the iced ones for the first five days of storage. Such a marked prevalence in quality for five days would suffice for the fish to fetch a premium in the market over other landings of the same fish whether chilled or not chilled. Chilling on board in CSW and icing the same after landings, did not show encouraging results.

Preservation of Indian oil sardine (Sardinella longiceps) in iced and chilled seawater. Part 2 - Changes during storage with particular reference to salt penetration and lipid deterioration during CSW holding

Oil sardines in prime condition were chilled on board. Two lots were chilled in CSW (samples C & CI), one lot ice (sample I) and a fourth lot was left un-iced on deck (sample AI). Sample AI was iced after landing and sample CI was taken out of the chilled seawater and. iced. All the four samples were kept in a chilled room for storage studies. Sample C, chilled and stored in CSW, recorded a gradual gain in weight and an increase in salt content of the muscle. Presence of salt did not seem to cause any excessive protein denaturation. Salt extractability decreased at a gradual rate in all cases. Presence of salt seemed to wield no noticeable influence on lipid hydrolysis and subsequent peroxidation. Results of chemical and sensory evaluations highlight this. Holding sardines in CSW gave a product of excellent quality for the first four to five days of storage. Beyond the fifth day of storage quality deteriorated rapidly and there was no noticeable superiority for this sample (sample C) over the on board iced fish. This was evident in the sensory evaluation as well. However, a storage life of five days in a readily acceptable state is sufficient for the fish to be disposed in the market at a premium sale price over other landings of the same species.

The preservation of some Indian freshwater fish

Quality changes in Labeo rohita, Cirrhinus mrigala and Tilapia mossambica were studied during storage in ice and at ambient temperature (29-32°C). After 10h at ambient temperature L. rohita and T. mossambica were completely spoiled, while C. mrigala became unacceptable within 11h. Organoleptic examination, total volatile base nitrogen and total bacterial counts showed that L. rohita (0.5 kg and above) and C. mrigala (0.5 kg and above) were acceptable up to 7 to 8 days of storage in ice in popular container (Bamboo basket) lined with palmyrah mat) at ambient temperature while T. mossambica (30-40 g) was acceptable up to 6 days. Influence of size of fish on shelf life in ice studied with reference to L. rohita showed that fishes below 500 g were acceptable up to 6 days of storage in ice in popular container at ambient temperature while those above 1000 g size were acceptable up to 8 days. Provision of an alkathene lining to the popular container extended the storage life of L. rohita from 6 days to 8 days. Pre-chilled and iced L. rohita had a longer shel flife (9 days) than merely iced sample (7 days).

Methods of harvesting and preservation of the diatom Chaetoceros calcitrans

Diatom culture and larval feeding experiments were conducted to test the viability and acceptability of preserved algal concentrates. C. calcitrans is characterised by the presence of setae which keep them suspended in cultures and make autoflocculation very difficult. Flocculation was induced by the addition of a floc-forming chemical. Using the optimum conditions, it was possible to harvest the algae within 1-h settling time and with about 84% recovery. The viability of frozen Chaetoceros was determined by actual cell reproduction. Preliminary feeding experiments showed that Chaetoceros can be successfully used as a substitute for fresh diatoms as feed for Penaeus monodon larvae. Simple freezing techniques, with or without the use of protectants has been found convenient for preserving algal concentrates in small volumes for both feeding and culture purposes.

A comparative study of various extenders of milkfish, Chanos chanos (Forsskal) sperm preservation

Four chemical extenders in 7 different concentrations (potassium chloride, sodium chloride, glucose, sodium citrate, Ringer s solution, cow serum and milkfish (Chanos chanos) serum) were compared in the preservation of milkfish sperm. Results showed milkfish serum to be the most suitable of the various extenders tested. This may be attributed to suitable osmotic potential and/or presence of proteins which may have directly or indirectly influenced sperm viability. The effects of milkfish serum on the motility and fertilizing capacity of sperm at different durations of storage however need to be investigated.

The effect of artisanal preservation methods on nutritional security of “Mukene” Rastrineobola argentea caught from Lakes Victoria and Kyoga in Uganda

The artisanal fish preservation methods in Uganda are characterized by extreme operating conditions. Consequently, vital nutritional components diminish in value and quantity which renders fish consumer nutritionally insecure. To establish the magnitude of nutritional loss, duplicate samples of Mukene Rastrineobola argentea were collected from Kiyindi landing site on L. Victoria and Moone landing site on L. Kyoga. Each set of duplicate samples was divided into five portions and kept on ice. For each preservation method a portion was processed into respective products at Food Bioscience and Agri-Business Laboratories aside from the control (fresh) sample. Both preserved and control samples were analysed for nutrient loss at Department of Chemistry, Makerere University using AOAC methods. The composition of fatty acids was determined by methanolysis gas chromatography and Mass spectrophotometry of the resultant methyl esters. The results indicate that nutrients of all preserved samples did not vary significantly from the control except for some fatty acids. The Eicosapentaenoic acid (EPA) in fresh samples declined from 6.72% to 1.08% in deep-fried samples constituting 83.93% nutrient loss. The sum ratio w3:w6 as well as EPA: DHA (Docosahexaenoic) ratio in fried samples also varied significantly (p<0.5) lower than 0.668 and 0.20 for the average of either preservation methods and experts recommended ratio respectively. Further research has been recommended to ascertain the causative factor, since Mukene frying is being promoted in the Great lakes region as alternative method to sun-drying. In conclusion, regular consumers of fried Mukene do not benefit much from the nutritional and health attributes of Omega 3 and 6.

The preservation of some East African freshwater fish

Quality changes during storage were investigated for several commercially important East African freshwater fish. Lates, Bagrus, Protopterus, Tilapia esculenta and T. nilotica were examined during storage in ice and at ambient temperature (250•C). After 24 hours at ambient temperature Lates and Bagrus were completely spoilt but Protopterus was still edible. In iced storage most fish were acceptable for at least 20 days. Organoleptic examination showed that T. nilotica was acceptable after 22 days storage in ice and that gutting was only marginally beneficial. Changes in physical appearance, which could form the basis of a fish inspection system, were recorded during storage. Possible chemical quality control indices were also investigated. It was found that total volatile bases and hypoxanthine are unlikely to be useful quality indices for the species studied with the possible exception of Lates. The bacterial counts of the flesh and skin of T. esculenta and T. niloticus were found to be low (a maximum of 10 organisms per sq cm of skin or per g of flesh) after 22 days storage in ice.