Predicting the distribution of Vibrio vulnificus in Chesapeake Bay

Vibrio vulnificus is a gram-negative pathogenic bacterium endemic to coastal waters worldwide, and a leading cause of seafood related mortality. Because of human health concerns, understanding the ecology of the species and potentially predicting its distribution is of great importance. We evaluated and applied a previously published qPCR assay to water samples (n = 235) collected from the main-stem of the Chesapeake Bay (2007 – 2008) by Maryland and Virginia State water quality monitoring programs. Results confirmed strong relationships between the likelihood of Vibrio vulnificus presence and both temperature and salinity that were used to develop a logistic regression model. The habitat model demonstrated a high degree of concordance (93%), and robustness as subsequent bootstrapping (n=1000) did not change model output (P > 0.05). We forced this empirical habitat model with temperature and salinity predictions generated by a regional hydrodynamic modeling system to demonstrate its utility in future pathogen forecasting efforts in the Chesapeake Bay.

Larvae of Dactylopsaron dimorphicum (Perciformes: Percophidae) from oceanic islands in the southeast Pacific

Percophids are a family of small marine benthic fishes common over soft bottoms from inshore to the outer slopes in tropical to temperate regions of the Atlantic and in the Indo-West and southeast Pacific (Reader and Neira, 1998; Okiyama, 2000). Five species belonging to four genera have been recorded around the Salas y Gómez Ridge in the southeast Pacific, all of which are endemic to the area except for Chrionema chryseres, a species which also occurs off the Hawaiian Islands and Japan (Parin, 1985, 1990; Parin et al., 1997). Of these five species, larval stages have been described only for Osopsaron karlik and Chrionema pallidum (Belyanina 1989, 1990).

Some parasitic Copepoda from Sri Lanka (Ceylon) with a synopsis of parasitic Crustacea from Ceylonese freshwater fishes

This report comprises a summary of parasitic copepods from fishes in Ceylon, as isolated from the branchial material of fishes belonging to previous collections. Seven copepod species are described in detail, as well as one species of Branchiura and one species of Isopoda. Caution is advocated to avoid further introductions via parasite-infested fishes, since only four of the above species are endemic.

Results of the Austrian-Ceylonese hydrobiological mission 1970 of the 1st Zoological Institute of the University of Vienna (Austria) and the Department of Zoology of the Vidyalankara University of Ceylon, Kelaniya. Pt. 7. Dryopidae and Elminthidae of Ceylon

This report comprises a summary of parasitic copepods from fishes in Ceylon, as isolated from the branchial material of fishes belonging to previous collections. Seven copepod species are described in detail, as well as one species of Branchiura and one species of Isopoda. Caution is advocated to avoid further introductions via parasite-infested fishes, since only four of the above species are endemic.

An analysis of records of gillnet fishing in Pilkington Bay, Lake Victoria

Among the commercially valuable species occurring in Lake Victoria the two endemic species of Tilapia, T. esculenta (Graham) and T. variabilis (Boulenger) still provide the basis for the fishery in many areas, though in some places the non-cichlid species are more important, for example in the Kagera river area where large numbers of Labeo victorianus and Schilbe mystus migrate seasonally into the river. Although certain species show these habitat preferences. Most of the coastline of Lake Victoria supports a wide variety of species, all of which contribute to the commercial fishery. Over the past ten years the East African Fisheries Research Organisation has fished experimentally a number of stations within a radius of fifty miles from Jinja. These stations have been selected so as to cover a variety of habitats which range from sheltered bays to exposed coastlines. The records discussed in this paper are the result of fishing operations carried out in Pilkington Bay over the period December 1954 to November 1956. This series of fishing operations was carried out in an attempt to assess the composition of the fish populations contained in this area. To this end a variety of nets of differing mesh sizes were used. Pilkington Bay is about ten square miles in extent and is an irregular and deep indentation on the north coast of Buvuma Island. It lies within the sleeping siclmess area where fishing by Africans is prohibited. When selecting an experimental fishing ground, it is important to have some idea of the amount of commercial fishing being carried out in the area. Throughout the period of this experiment a few African fishermen were seen operating illegally in the area, but it is unlikely that their efforts greatly affected the results obtained. Pilkington Bay was fished by E.A.F.R.O. in 1949 and 1950 (Lowe McConnell 1956) but since then till the present experiment, fishing in the area has been infrequent.

The marine linefish resources of Moçambique (status, development and future research)

Marine line-fishing is seen to be of major importance to the social well-being and economy of many coastal people of Mozambique. Aspects of the artisanal and semi-industrial fisheries of Mozambique are described and a recent significant increase in effort is noted. Landings are seen to comprise a high proportion of vulnerable, endemic species, several of which are shared with neighbouring South Africa. Trends in CPUE, sex ratios and yield per recruit suggest that future landings may decline if conservative management is not introduced. Strategies for data collection and biological research on key species are proposed.

A study on the piscifauna of the Idukki Reservoir and catchment area

From the area under report 17 species, 15 endemic and 2 exotic, of freshwater fish have been identified. Of these, 8 species are commonly found in the catches and are of fishery significance. The fact that small fish species which have no fishery importance also support life in other trophic levels of this ecosystem is well exemplified by the interaction of the birds and mammals with these species. A scientific management and monitoring of the reservoir waters as well as the remaining segments of forests are recommended to salvage the wild life and vegetation from a possible rapid deterioration within years.

Vegetation structure and faunal composition of Wathurana wetland ecosystem Tebuwana, Kalutara, Sri Lanka

A study was conducted in Tebuwana Wathurana Wetland ecosystem to understand its vegetation structure and faunal composition in order to assess its conservation needs. As there are no published records on the flora and fauna of Wathurana Wetlands in Tebuwana, it is necessary to understand the ecological and other relevant features in order to develop strategies to conserve this wetland. These objectives were pursued by surveying the vegetation of the wetland and by identifying fish and bird species present. A total of 66 species of flora and 61 species of fauna were identified in the survey. Of the 27 fish species recorded from the Tebuwana Wetland, 9 species were endemic and 17 species belonged to the indigenous category. With regard to the flora in the wetlands, the dominant families were Rubaceae, Fabaceae and Arecaceae. The 66 species belonged to 39 families and 61 genera while 12 species were endemic and 4 species were considered highly threatened. These flora were found in four layers. Of the 22 species of birds recorded, two species were endemic. This study revealed that these Wathurana Wetlands have a high species diversity but that they face many threats including encroachments, extraction of forest products mainly as timber, land filling, mining and occurrence of invasive species. It is essential to minimize the exploitation of natural resources from this wetland in the future and in particular to mark the boundary, conduct awareness programmes and continue research.

Examining the possibilty of multiplication and breeding of (Schizothorav [sic] zarudnyi) in order to restoration of regional aquatics reservations of Hamoon Lake and taking its advantag [sic] for aquaculture

Schizothorax zarudnvi, is an endemic fish of east country waters. (Triple lagoons of Hamoon and relevant water resources) that in the world it is reported in this resource specially. This fish named Hamoon mahi is one of the most economically valuable species in this region. Because of the recent years droughts, Hamoon logoon has been drive since 2000. Also, semi-wells (a semi natural resource) were affected drastically by recent drought years and their volume reduced to nearly one third of their real volume and resulted in changing at growth and reproduction physiology process in Schizothorax zanidnyi, brood stocks. Beginning of this project was done from October 2003. It's field studies begun (brood catching) since November 2001 by two methods including entangling gairs and at semi wells of Sistan that (Beach seine) had maximum rate of preparing qualified brood stocks. Broods transferred to Cyprinidea reproduction work shop of Zahak and after taking primary measures they stored in to the edaphic pools. Increasing the success safety factor (coefficient) for artificial reproduction of Sthizothorax zarudnyi , identifying the appropriate tune for Hormonal acceptance (physiological preparation of broods) is needed , so this important work was done regularly by histological studies and GSI measurements since November. Highest GSI rates of females (%80.51) and highest IV stage abundance of sexual maturity (%l 00) were observed an march. On the base of this date, Hormone therapy was done on broods on march. The used hormones are as follows Hypophysis. extraction, GnRHa and Anti Dopamin at the dozes of 3-6 ml, 20-30kg and 10-15 ml per kg body weight respectively and 2-3 times from 11-12-80 they were injected. Injected broods kept in to two circumstances, flow-through (rounded pool) and stagnant systems. In stagnant system 14 and 19 individuals of female and male (Schizothorax zauiulnri) broods, respectively injected in 11th, 15111, 19th, and 24th of march 1380. Non of the injected broods in 11 and 15 and 19th march (in stagnant Condition) answered to Hormone therapy. After final injection broods had general less activity and a few of them died. Mean temperature of brood pond waters (daily) which were injected. Fluctuated between 10-25-13. 63°c but injected broods on 24th march had different characteristics. They had pale color and had few fecundity. In this stage of injection they hadn't any successful vulation. After injection, Mean daily water temperature was 15, 88-17, 54°c. In Flowing system, 13-16 individual of males and females respectively were injected on 15th, 19th, 22th and 23th march. None of injected producers on 15th and 19th march with mean daily water temperature of 10, 25-12°c were prepared for spawning but injected producers on 22nd an 23th march with mean daily water temperature of 13.5-1 rc responded about 75-100 percent. (Schizothorax zarudnyi) brood stocks were prepared for spawning after 353-428 hours/day from final injection. Diameter of obtained eggs (before fertilization) was between 1.9-2.3 min and of fertilized eggs was 3.8mm. Fertilized eggs of (Schizothorax zarudnyi) were hatched after 6-7 days with mean water temperature of 17.08°c. Mean length of on one day larvae was 9.47 mm. Larvae was 9.47 mm. Larvae adsorbed the whole yolk sac after , 5-6 days at 17- 1°c and were prepared for releasing in to edaphic pools. Because of the lack of necessary and complementary facilities in the region , they had to release them in to veniros and growing them for 8 days. At the end of 18th day , 35000 larvae (at first) released into an edaphic pond with a volume of 150m2. After growing them for one moth , mean length and weight of new hatched larvae was 29.41 mm and 1.12►r , respectively. With respect to results of this investigation , artificial reproduction of (Schizothorax zarudnyi) Can be possible at 14-17°C and flowing water with Hormonal treatment. It -s breeding has increased development than other cultural specious in the region. Due to high economical value of this specious in Sistan and ti-s specialization east waters of Iran and having high resistance and proper growth There is a need of it's development and reproduction and culture in fish culture fanns (edaphic ponds• two-purpose pools) at the region and country.

Geographic and economic setting of Lake Victoria

Lake Victoria, besides being the second largest in the world after Lake Superior, is the largest tropical lake. Its waters are shared by Kenya (6% of the surface area), Uganda (43%), and Tanzania (51%). Before dramatic structural and functional changes manifested in the lake's ecosystem especially in the 1980s, fish life flourished in the lake's entire water column at all times of the year. Currently, the situation is much more different from what it was in the past. The exponential increase in the introduced Nile perch (Lates niloticus) and Nile tilapia (Oreochromis niloticus) stocks, siltation, wetland degradation and eutrophication have characterised the lake ecosystem. The two exotic species and the small native cyprinid (Rastrineobola argentea) form the basis of the commercial fishery that was once dominated by two native tilapiines (Oreochromis esculentus and Oreochromis variabilis) and five other large-bodied endemic fishes. Severe deoxygenation observed at shallow depths (Ochumba 1990; Hecky et al., 1994) indicates that a large volume of the lake is unable to sustain fish life. The Lake Victoria catchment is one of the most densely populated areas in East Africa, encompassing a population of about 30 million people. Widespread poverty resulting from high inflation rates, lack of opportunities and general unemployment have characterised the lakeside communities over much of the last two decades. The biophysical environment in which Lake Victoria exists makes the lake particularly susceptible to changes that occur as a result of human modification to the watershed or the lake itself, thus rendering benefits from the lake unsustainable.

The history of fish communities, biodiversity and environment of Lake Victoria and the lessons learnt

The first fishery survey of Lake Victoria was conducted between 1927 and 1928 (Graham 1929). Atthat time, the lake had a diverse fish fauna and the fishery was dominated by two endemic tilapiine cichlids; Oreochromis esculentus (Graham 1929) and O. variabilis (Boulenger 1906). There were a number of other species such as Protopterus aethiopicus Heckel 1851, Bagrus docmac (Forsk.) 1775, Clarias gariepinus (Burchell), Barbus species, mormyrids, Synodontis spp, Schilbe intermedius (Linn.) 1762 and Rastrineobola argentea Pellegrin, 1904 that were also abundant in the lake most of which made a significant contribution to the fishery (Graham 1929, Worthington 1929, 1932, Kudhongania & Cordone 1974). Haplochromine cichlids were represented by at least 300 species more than 99% of them endemic (Greenwood, 1974; Witte et al., 1992 a & b). The fishery of Lake Victoria was similar to that of lakes Kyoga and Nabugabo (Worthington 1929; Trewavas 1933; Greenwood 1965, 1966; Beadle 1962, 1981). There were also important fisheries on the inflowing rivers of Lake Victoria, the most important of which were Labeo victorianus and Barbus altianalis (Cadwallader 1965). The small sized species notably Rastrineobola argentea and haplochromines cichlids were not originally commercially exploited.

Evolution of the tilapia fishery with specific reference to the Nile tilapia (Oreochromis niloticus Linne)

As a fishery, the immensely large (c. 68,800 km2 ) Lake Victoria is a unique ecosystem which together with a riverine connection to the Lake Kyoga basin share a common endemic "Victorian" fish fauna (Greenwood 1966). Until the 1950s, the single socio economically most important species of fish in these two lakes was the native Oreochromis esculentus Graham (Graham 1929) even though the lake also contained a second native tilapiine, 0reochromis variabilis , and over 300 other fish species (Beauchamp, 1956).

Distribution of endangered native fish fauna and their conservation in Lake Victoria

Lake Victoria in East Africa, supports socio-economically important fisheries for more than 30 million inhabitants in the lake basin. The lake had until the 1970's a diverse fish assemblage dominated by haplochromines species which formed at least 83% of the fish biomass (Kudhongania & Cordone 1974). The more than 500 haplochromine species in Lake Victoria, over 99% of them endemic, exploited virtually all the food sources in the lake (Witte and van Oijen 1990). Each species had its own unique combination of food and habitat preference (Goldschmidt et al., 1990).

The major aquatic systems of the Victoria and Kyoga lake basins

The Victoria and Kyoga lake basins form the major aquatic system of this study (Fig. I). The two lake basins share a common evolutionary history and have similar native fish faunas (Graham 1929, Worthington 1929). The two main lakes have also had similar impacts by introduction of Nile perch Lates niloticus and therefore these two lakes can be considered to be similar for ichiogeographical purposes. These lake basins have many satellite lakes isolated from one another and from the main lakes Victoria and Kyoga by swamps and other barriers. Some of these satellite lakes still possess stocks of endemic fish species which are almost extinct from the main water bodies. It was therefore considered that understanding of these lakes would contribute to the knowledge base required to solve some of the problems experienced in Lake Victoria and Kyoga especially the loss in trophic diversity arising. The study was carried out in these two main water bodies (Kyoga and Victoria) and on other satellite lakes e.g Wamala, Kachera, Mburo, Kayanja and Kayugi in the Victoria lake basin and lakes Nawampasa, Nyaguo, Agu, Gigate, Lemwa and Kawi in the Kyoga lake basin (Figs. 2, 3, 4, 5 & 6).

Trophic interrelationships and food-webs among the fishes in ecosystems of the Victoria and Kyoga lake basin

The Victoria and Kyoga lake basins had a high fish species diversity with many fish species that were found only in these lakes. Two Tilapiines species Oreochromis esculentus and Oreochromis variabilis were the most important commercial species in these lakes and were found nowhere else on earth except in the Victoria and Kyoga lake basins (Graham 1929, Worthington 1929). Lakes Kyoga and Nabugabo also had endemic haplochromine species (Worthington 1929, Trewavas 1933, Greenwood 1965, 1966). As stocks of introduced species increased, stocks of most of the native species declined rapidly or disappeared altogether. The study was carried out on Lakes Victoria and Kyoga, River Nile, some selected satellite lakes from the two basins namely Lakes Mburo, Kachera, Wamala, Kayanja, Kayugi, Nabugabo, Victoria, Victoria nile and River Sio(Victoria lake basin). Lakes Kyoga (Iyingo), Nawampasa, Nakuwa, Gigati, Nyaguo, Agu, Kawi and Lemwa (Kyoga lake basin). Species composillon and relative abundance of fishes were estimated by detennining the overall average total number of each species encountered. A trophic consists of species using the same food category. Shannon-Weaver Index of diversity H (Pielou, 1969) and number of trophic groups, were used to estimate the Trophic diversity of various fish species in the lakes. Food analysis has been done on some fishes in some of the sampled lakes and is still going on, on remaining fishes and in some lakes. Generally fish ingested detritus, Spirulina, Melosira, filamentous algae, Planktolyngbya, Microcysists, Anabaena, Merismopedia, Spirogyra, higher plant material, rotifers, Ostracodes, Chironomid larvae and pupae, Choaborus larvae, Odonata, Povilla, Insect remains, Caridina, fish eggs and fish. Eight trophic groups were identified from thes food items ingestes. These included detritivores, algae eaters, higher plant eaters, zooplanktivores, insectivores, molluscivores, prawn eaters, paedophages and piscivores. Trophic diversity by number of trophic groups was highest in Lake Kyoga (6) followed by lakes Kayugi, Nabugabo, River Nile and Mburo (3) and the lowest number was recorded in kachera (2).