Artificial fertilization of the eggs, and rearing and identification of the larvae of the anchoveta, Cetengraulis mysticetus

ENGLISH: The anchoveta, Cetengraulis mysticetus (Günther), is an important bait fish used to capture tunas in the Eastern Tropical Pacific Ocean. Contributions to the early life history of this species in the Gulf of Panama were made by Simpson (1959), who was able to identify deductively the planktonic egg of the anchoveta from 10 other anchovy eggs concurrently present. He also reared these planktonic eggs in the laboratory and described the resultant larvae to the age of 48 hours after hatching. Because of the lack of differences among the anchovy larvae, this description does not permit the identification of anchoveta larvae from those of other engraulid species. Furthermore, while adult specimens are easily recognized, up to the present it has not been possible to extend the identification of the juvenile anchoveta to specimens smaller than about 25 mm. The purpose of this study, therefore, was to identify anchoveta from the time of hatching to about 25 mm. SPANISH: La anchoveta, Cetengraulis mysticetus (Günther), es un importante pez de carnada que se emplea en la captura de los atunes en el Océano Pacífico Oriental Tropical. Simpson (1959) logró identificar deductivamente el huevo planctónico de la anchoveta al separarlo de otros diez huevos de anchoas que se encuentran al mismo tiempo, contribuyendo de esta manera a conocer los primeros estados de la historia natural de esta especie en el Golfo de Panamá. El también estableció un criadero en el laboratorio con estos huevos planctónicos y describió las larvas resultantes hasta la edad de 48 horas después de la eclosión. Debido a que no hay diferencias entre las larvas de las anchoas, esta descripción no permite identificar las larvas de la anchoveta de las otras especies de engráulidos. Más aun, a pesar de que los especímenes adultos son fácilmente reconocibles, hasta ahora no ha sido posible identificar la anchoveta juvenil de menos de unos 25 mm. Consecuentemente, el propósito del presente estudio ha sido el de identificar al anchoveta desde el momento de la eclosión hasta que tiene unos 25 mm.

Effects of suspended sediments on the development of eggs and larvae of striped bass and white perch

The possible ecological effects of suspended sediments are manifold. Briefly, suspended sediments may cause an increased surface for microorganism growth, fewer temperature fluctuations, chemical adsorption or absorption, blanketing, mechanical-abrasive actions, and light penetration reduction (Cairns, 1968). Sherk and Cronin (1970) have pointed out that the above effects have been little studied in the estuarine environment. The ecological effects of suspended sediments on fish eggs and larvae may be of prime importance t o the C and D Canal area, an important spawning and primary nursery area for a variety of estuary: e species (Johnson,1972). This section discusses the effects of suspended sediment on the eggs and larvae of striped bass and white perch.

Eggs, Larvae and Young of The Striped Bass, Roccus saxatilis

Detailed descriptions of the early development of the striped bass, Roccus saxitilis (Walbaum), with emphasis on variation in size and morphology, sequence of fin formation, changes in body form, and attainment of the full complement of maristic numbers, are presented and illustrated for the first time. The egg is spherical, transparent, non-adhesive and relatively large. It is pelagic and buoyant, although it sinks in quiet fresh water. When unfertilized, it averages 1.3 mm, in diameter, but is 3.4 mm. when fertilized and water-hardened. The granular yolk sac, green when alive and whitish-yellow when preserved, averages 1.2 mm., and the single amber-colored oil globule is about 0.6 mm. in diameter. Newly hatched striped bass prolarvae, which range from 2.9-3.7 mm. in total length, are relatively undeveloped and nearly transparent, with no mouth opening, unpigmented eyes, and a greatly enlarged yolk sac with the large oil globule projecting beyond the head. When 5-6 mm. long the yolk sac and oil globule are assimilated and the postlarvae I show advanced development of the internal anatomy. Although the fish is still transparent, scattered melanophores are found on the head and body and chromatophores in the eyes and the ventro-posterior edge of the body. Postlarvae transform to young between 7 and 10 mm. in length when the finfolds are lost except in the dorsal, anal and caudal regions. The largest fish in this group possess a well-formed skeleton with a full complement of 25 vertebrae. Between 10 and 20 mm. in length all fish are fully transformed, muscular tissue renders most of the internal structure obscure, and the myotomes, which generally correspond in number with the vertebrae, are no longer visible. At fish lengths of 20-30 mm. scales are found on all specimens, and with the exception of the pectoral fin-rays, a full complement of meristic structures is present in all other fins. At this stage the body is pigmented uniformly with small spots. Linear regressions between several dependent variables and the , independent variable of standard length indicate that the rate of development of head, eye. and snout to anus lengths is proportional to the length of the larvae and young. Body depth and standard length are non-linear among newly-hatched larvae. Hatchery-reared striped bass demonstrated a slow rate of growth, and were regarded as "stunted," when compared to growth rates observed in another study and field collections. Observations were also made on abnormal eggs and teratological larvae and young. Blue-sac disease is tentatively identified and described for the first time in larvae and pugnosed larvae and young are also described for the first time in striped bass.

An identification key of the most important German freshwater teleost fishes by means of their eggs [Translation from: Archiv fur Hydrobiologie 83(2), 200-212, 1978]

The fetal and larval development of many freshwater fish is already relatively well covered. Coverage of the morphology of fish-species' eggs is very sparse. For this reason the authors have attempted to prepare a key on fish eggs which covers the bulk of German Teleostei fish. The key also includes a discussion of problems of categorization and terminology.

The life capacity of copepod's eggs passed through the gut of Ladoga lake Coregonus [Translation from: Zool.Journal.Moscow 29-5, 476-477, 1950]

This short paper summarises findings of experiments that were carried out using copepod eggs taken from guts of Coregonus which breeds in various Ural lakes. The study showed that copepod eggs can pass through the gut of Coregonus unharmed.

Observations on the physiological development of trout eggs [Translation from: Roux. Arch. f. Entwicklungsmechanik der Organismen, 114. 771, 1929]

The osmotic pressure of the perivitelline fluid and the yolk of trout (Salmo trutta) eggs were measured separately by the Drucker-Schrein method. The permeability of the egg membrane and the variations in the osmotic pressure of the eggs when placed in salt solutions were also investigated.

Some observations on washout of brown trout (Salmo trutta L.) eggs in Teesdale streams

Brown trout (Salmo trutta) in Teesdale lay their eggs in the streambed gravels in the Autumn, here the eggs slowly develop to emerge as young fry in the Spring. Whilst the eggs are in the gravel they are vulnerable to displacement by high water velocities. Eggs removed in this way are not thought to remain viable since they are very susceptible to death through physical shock - especially in the earlier stages of development. Streams in Teesdale are known to be amongst the most flashy in England and thus are good sites in which to study egg washout. Three field sites were used for the study of egg washout in Teesdale - Great Eggleshope, Thorsgill and Carl becks. This report describes preliminary studies of a varied nature into this subject from which an attempt is made to assess the importance of egg washout to the survival of brown trout in Teesdale.

Aspects of the washout of salmonid eggs. 1. Observations on the physical characteristics of real and artificial eggs, their rate of fall in a water column and their pattern of settlement in an experimental channel

When salmonid redds are disrupted by spates, the displaced eggs will drift downstream. The mean distance of travel, the types of locations in which the eggs resettle and the depth of reburial of displaced eggs are not known. Investigation of these topics under field conditions presents considerable practical problems, though the use of artificial eggs might help to overcome some of them. Attempts to assess the similarities and/or differences in performance between real and artificial eggs are essential before artificial eggs can validly be used to simulate real eggs. The present report first compares the two types of egg in terms of their measurable physical characteristics (e.g. dimensions and density). The rate at which eggs fall in still water will relate to the rate at which they are likely to resettle in flowing water in the field. As the rate of fall will be influenced by a number of additional factors (e.g. shape and surface texture) which are not easily measured directly, the rates of fall of the two types of egg have been compared directly under controlled conditions. Finally, comparisons of the pattern of settlement of the two types of egg in flowing water in an experimental channel have been made. Although the work was primarily aimed at testing the value of artificial eggs as a simulation of real eggs, several side issues more directly concerned with the properties of real eggs and the likely distance of drift in natural streams have also been explored. This is the first of three reports made on this topic by the author in 1984.

Aspects of the washout of salmonid eggs. 3. Observations on the settlement of artifical eggs in a section of natural stream channel at several different discharges

It is of value to know the approximate distance of travel at different stream discharges and/or water velocities, of salmonid eggs which have been displaced from redds by spates. This report describes studies in 20 m of stream channel upstream of the fish trap in Dubby Sike. Observations were made on the relation- ships between discharge and water depth and velocity and also on the relationships between water velocity and the settlement of artificial trout eggs. The main aim was to test the hypothesis that, at any given water velocity, eggs would drift smaller distances in a natural stream than in the experimental channels.

Aspects of the washout of salmonid eggs. 6. Information on gravel composition , redd sites, the dimensions of redds and the depth of egg burial

Sampling was concentrated on the North Moor region and the series of ditches which drained this area to the Bristol Channel. Although most ditches were not deep the mud substratum precluded sampling from within the habitat. All samples were taken with a pond net from the banks. Efforts were made to sample each part of the habitat although in some ditches the macrophyte growth was so intense as to make sampling difficult particularly of the sediments. Organisms were identified on the 10 sampling sites.

Aspects of the washout of salmonid eggs. 5. Attempts to assess the importance of washout as a cause of mortailty

The paper reviews the methodology of attempts to assess the importance of washout as a cause of loss of salmonid eggs and alevins. The results of this study are presented of various small-scale field trials using buried artificial salmonid eggs and tethered table tennis balls. The results suggested that, even when few eggs were actually lost by washout, some downstream movement of the upper layers of gravel and of artificial eggs might have taken place.

Mussel eggs as indicators of mutagen exposure in coastal and estuarine environments

The aim of this study was to develop a short-term genotoxicity assay for monitoring the marine environment for mutagens. Based on the developing eggs and embryos of the marine mussel Mytilus edulis, an important pollution indicator species, the test employs the sensitive sister chromatid exchange (SCE) technique as its end-point, and exploits the potential of mussel eggs to accumulate mutagenic pollutants from the surrounding sea water. Mussel eggs take up to 6 months to develop while in the gonad, which provides scope for DNA damage to be accumulated over an extended time interval; chromosome damage is subsequently visualised as SCEs in 2-cell-stage embryos after these have been spawned in the laboratory. Methods which measure biological responses to pollutant exposure are able to integrate all the factors (internal and external) which contribute to the exposure. The new cytogenetic assay allows the effects of adult exposure to be interpreted in cells destined to become part of the next generation.

Stage-specific vertical distribution of Alaska plaice (Pleuronectes quadrituberculatus) eggs in the eastern Bering Sea

The stage-specific distribution of Alaska plaice (Pleuronectes quadrituberculatus) eggs in the southeastern Bering Sea was examined with collections made in mid-May in 2002, 2003, 2005, and 2006. Eggs in the early stages of development were found primarily offshore of the 40-m isobath. Eggs in the middle and late stages of development were found inshore and offshore of the 40-m isobath. There was some evidence that early-stage eggs occur deeper in the water column than late-stage eggs, although year-to-year variability in that trend was observed. Most eggs were in the later stages of development; therefore the majority of spawning is estimated to have occurred a few weeks before collection—probably April—and may be highly synchronized among local spawning areas. Results indicate that sampling with continuous underway fish egg collectors(CUFES) should be supplemented with sampling of the entire water column to ensure adequate samples of all egg stages of Alaska plaice. Data presented offer new information on the stage-dependent horizontal and vertical distribution of Alaska plaice eggs in the Bering Sea and provide further evidence that the early life history stages of this species are vulnerable to near-surface variations in hydrographical conditions and climate forcing.

Estimating the encounter rate of Atlantic capelin (Mallotus villosus) with fish eggs, based on stomach content analysis

The number of pelagic fish eggs (cod and cunner) found in stomachs of capelin (Mallotus villosus) sampled in coastal Newfoundland was used to estimate the encounter rates between capelin and prey, and thus the effective volume swept by capelin. Fish eggs were found in 4−8% of capelin stomachs, represented an average of 1% of prey by numbers, and their abundance increased as relative stomach fullness decreased. The average number of eggs per stomach doubled for each 5-cm increase in length of capelin. The effective volume swept for eggs by capelin ranged from 0.04 to 0.84 m3/h—a rate that implies either very slow capelin swimming speeds (<1 cm/s) or that fish eggs are not strongly selected as prey. The predation rate estimated from stomach contents was higher than that predicted from laboratory studies of feeding pelagic fish and lower than that predicted by a simple foraging model. It remains uncertain whether capelin play an important regulatory role in the dynamics of early life stages of other fish.

Temporal and spatial distribution and abundance of flathead sole (Hippoglossoides elassodon) eggs and larvae in the western Gulf of Alaska

Data from ichthyoplankton surveys conducted in 1972 and from 1977 to 1999 (no data were collected in 1980) by the Alaska Fisheries Science Center (NOAA, NMFS) in the western Gulf of Alaska were used to examine the timing of spawning, geographic distribution and abundance, and the vertical distribution of eggs and larvae of flathead sole (Hippoglossoides elassodon). In the western Gulf of Alaska, flathead sole spawning began in early April and peaked from early to mid-May on the continental shelf. It progressed in a southwesterly direction along the Alaska Peninsula where three main areas of flathead sole spawning were indentified: near the Kenai Peninsula, in Shelikof Strait, and between the Shumagin Islands and Unimak Island. Flathead sole eggs are pelagic, and their depth distribution may be a function of their developmental stage. Data from MOCNESS tows indicated that eggs sink near time of hatching and the larvae rise to the surface to feed. The geographic distribution of larvae followed a pattern similar to the distribution of eggs, only it shifted about one month later. Larval abundance peaked from early to mid-June in the southern portion of Shelikof Strait. Biological and environmental factors may help to retain flathead sole larvae on the continental shelf near their juvenile nursery areas.