Technical report on the environmental monitoring of the cage area at the Source of the Nile (SON) Fish Farm for quarter 3: July-September 2011

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm requested for technical assistance of NaFIRRI to undertake regular environment monitoring of the cage site as is mandatory under the NEMA conditions. NAFIRRI agreed to undertake quarterly environment surveys in the cage area covering selected physical-chemical factors Like water column depth, water transparency, water column temperature, dissolved oxygen, pH and conductivity; nutrient status, algal and invertebrate communities (microinvertebrates/zooplankton and macro-invertebrates/macro-benthos) as well as fish community. The first quarter survey was undertaken in February 2011; the second in May 2011 and the third quarter survey, which is the subject of this report, in September 2011. Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and aq-uatic biota, including the natural fish community at and around the cage site.

Hydrobiological status of a lesser Himalayan river

It is generally observed that a variety of physical and chemical variables have considerable impact on the biological life in a mountain river which include plankton, benthic algae, benthic invertebrate and finally the fishery resources. They are often subjected to extreme hydrological disturbances particularly during rainy seasons when increased volume of water create 'Wash Off' situation for existing fauna and flora. A case study of a lesser Himalayan river - the Gaula, which drains the south-central part of Kumaon region in Uttaranchal is presented here. Since this river does not get snow-melt water, it mainly depends on steady flow of the groundwater round the year. The climate of the area is characterized with long winters, short summer and good amount of rain during monsoon months. The infiltrated groundwater which seeps into the surface soil layers during monsoon seasons is the chief source of discharge of water during winter and summer season

Phytoplankton communities of a floodplain lake of the Brahmaputra River basin, Upper Assam

Phytoplankton (52 species; Bacillariophyceae>Chlorophyceae>Cyanophyceae> Euglenophyceae=Dinophyceae) of Samuajan beel, a tropical floodplain lake, registered identical mean annual richness (30+4 species) in littoral and limnetic regions and depicted 33.3-77.2% and 31.4-81.1% community similarities respectively. Their abundance ranged between 137+54 n/l in littoral (Bacillariophyceae>Chlorophyceae) and 122 ± 45 n/l (Chlorophyceae>Bacillariophyceae) in limnetic communities, comprised about 46% of net plankton and indicated winter peaks. This study depicted moderate species diversity, high evenness and low dominance of phytoplankton; species diversity showed significant direct correlation with richness and evenness and an inverse relationship with dominance. Phytoplankton showed significant positive relationship with transparency and silicate and negative with water temperature, rainfall, chloride and nitrate. Multiple regression revealed that ten abiotic factors accounted for >80-98% of density variations of phytoplankton and the dominant groups. ANOVA depicted trends of significance in abundance of the biotic communities analysed.

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, September 2013

Source of the Nile Fish farm (SON) is located in northern Lake Victoria close to the headwaters of the River Nile. The proprietors of the farm have established a collaborative agreement with the National Fisheries Resources Research Institute (NaFIRRI) to undertake quarterly environment monitoring surveys of the fish cage site at Bugungu in the Napoleon Gulf. This activity is a mandatory requirement of the National Environment Management Authority (NEMA) of Uganda. Therefore NAFIRRI undertakes monitoring surveys once every quarter covering selected physical‐chemical parameters including water column depth, water transparency, water column temperature, dissolved oxygen, pH, conductivity and nutrient status; algal, zooplankton, macro‐benthos and fish communities. While the first quarter survey of 2013 (January‐March) was missed out due to late decision, the second quarter monitoring survey was dully undertaken in May 2013 and a technical report was compiled and submitted to the client. The present report covers the third quarter survey (July‐September) undertaken in September 2013. Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment quality and selected aquatic biota.

Benthic macro-biota in Gaula River - Kumaon Himalaya

The density of benthic macro-biota in number over weight of biomass at three sampling stations was 29 units m²/0.614 g m² to 171 units m²/11.346 m² at Station I; 22 units m²/0.410 g m² to 155 units m²/8.717 m² at Station II; 15 units m² 10.321 g m² to 122 units m²/6.793 g m² at Station III. The Caddis fly larvae were the most dominant component and contributed 52.41% in the macro-biota. These animals were abundant in Gaula River when benthic algae were abundant; water is well oxygenated, alkaline and contains sufficient nutrients but observed to be less abundant during high velocity of water, high river depth and higher turbid waters.

Early developmental stages of two Secutor species (Family: Leiognathidae) collected from the Bak-khali river estuary of the Bay of Bengal, Bangladesh

Early developmental stages of two Secutor species, Secutor insidiator (Bloch) (11.9-36.0 mm standard length, SL) and Secutor ruconius (Hamilton-Buchanan) (14.0-33.0 mm SL) collected by ichthyoplankton net from the Bak-khali river estuary of the Bay of Bengal, Cox's Bazar, Bangladesh are described and illustrated. All of the fins with supporting spines and rays were present in the smallest collected sizes of both species. With growth of the specimens, significant changes in melanophore patterns were found. S. insidiator is similar to S. ruconius in having upward protracting mouth parts and body colouration, but can be distinguished easily by its more elongate body shape (body depth 38-47% of SL compared with 46-52% of SL in Secutor ruconius). Both the species occurred round the year from August 1998 to July 1999. The surface water temperature and salinity during the study period varied from 22.0-32) C and 10-37 ppt respectively.

Effect of phosphorous supplementation in the formulated fish feed on carcass quality of Nile tilapia Oreochromis niloticus L.

A study was conducted to evaluate the effect of phosphorus supplementation in the formulated fish diet on carcass quality of Nile tilapia in net-cages suspended in fertilized earthen ponds. In the experiment 3% di-calcium phosphate (DCP), 3% triple supper phosphate (TSP) and 7% 16:20 inorganic fertilizer were added as phosphorous sources to three diets containing fish meal as main protein ingredient. Feeding tilapia in net-cages with these diets significantly (pNile tilapia over fish fed with same diet without phosphorous supplementation and fish given no feed. The final body composition and bone phosphorous content of Nile tilapia fed with DCP, TSP and 16:20 grade fertilizer supplemented diets were comparable.

Development of artificial breeding techniques for long-whiskered catfish, Sperata aor and giant river catfish, Sperata seenghala of Bangladesh

Sperata aor and S. seenghala are the two important native catfishes of Bangladesh but commercial farming of these species is not possible due to lack of naturally collected or artificially produced seeds for stocking. Attempts were made to develop techniques for seed production by artificial breeding and nursery-rearing of fries of these catfishes. A total of 60 S. seenghala (750-1,500 g) and 10 S. aor (600-1,000 g) broods were collected from the Brahmaputra river-basin and floodplains in Mymensingh region four months prior to their breeding season. The collected brood fishes were reared in separate earthen ponds with supplementary feeds comprising of rice bran (40%), mustard oil cake (29%), fish meal (30%) and vitamin-premix (1 %). Three experiments were conducted to optimize the hormone dose. A total of nine S. seenghala females weighing from 750 to 1,500 g were given an initial and resolving dose of 12-20 and 16-24 mg PG/kg body weight, respectively. The males weighing from 650-950 g were administered a single dose of 18-26 mg PG/kg body weight at the time of the time of administering the resolving dose to the females. The females ovulated partially and the eggs were examined under a compound microscope, but most of them were found to be less ripe or damaged. Collection of milt by stripping the males was not successful. The testes were taken out and sperm were observed to be non-motile and less developed. In view of stimulating natural propagation of S. seenghala, artificial holes (nests) were constructed in the pond bottom. Each hole was 0.7 m in diameter and 0.3 m in depth. A total of 10 holes were made and then 10 pairs of S. seenghala breeders (800-1,200 g) were stocked in the pond. In mid February, 3,000 fry of S. seenghala with a mean length of 4.60 cm and weight of 0.36 g were collected by repeated netting followed by drying of the pond. The fry were then stocked in a nursery pond and fed with commercial feed (SABINCO starter-1). The average length and weight of the fingerlings were 9.01 cm and 3.95 g, respectively and the estimated survival was 60% after two months of rearing. S. aor did not respond to natural spawning. Further study is essential to develop techniques for their successful artificial and natural breeding.

Quantitative and qualitative assessment of plankton: some ecological aspect and water quality parameters of the river Meghna, Bangladesh

Investigation on the seasonal distribution and abundance of various major taxa of phyto and zooplankton and the corresponding physico-chemical characteristics were carried out in four selected stations between the latitude 22°35.494N N-23°23.987 N and longitude 90°35.793 E- 90°49.061 E of the Meghna river system, Bangladesh. Drop count method was followed for the qualitative and quantitative analysis of both phyto- and zooplankton. A total of 41 phytoplankton genera belonging to 17 families and 13 zooplankton genera belonging to 11 families were recorded. Zooplankton growth cycle was noticeably less (3.0%) than the phytoplankton abundance almost throughout the study period. Quantity of plankton registered to increase chronologically from the upper to lower stretches of the river. During summer investigation the load of phytoplankton was recorded maximum (11,300-51,850 No/1). Ratio-wise quantitative difference between zoo- and phytoplankton in composition of the total standing crop fluctuated between 1.0:5.5 and 1:1037. Among the phytoplanktonic groups, Chlorophyceae was found to be dominating (95.0%) in all sampling stations. Protococcus, a single genus of Chlorophyceae played a unique role during summer, contributing the highest density of about 74.0%. The pattern of qualitative and quantitative difference of plankton standing crop in different sampling sites can be attributed to the existing physico-chemical characteristics, mainly water temperature, pH and hardness.

Effect of salinity on food consumption and growth of juvenile Nile tilapia (Oreochromi niloticus L.)

The effect of salinity (0, lO and 20%o, water temperature 28 ± l oC) on food consumption and growth of juvenile Nile tilapia, Oreochromis niloticus L. (9.94 ± 0.15 g) were investigated by feeding group of 20 fish at 2% body weight day. Individual food consumption was measured using X-radiography. There were no significant differences in growth or white muscle protein concentrations among groups. During feed deprivation, weight loss was similar for fish held at O%o and 10 %o salinity, but after 7 days over 50% of the fish maintained at 20%o salinity developed lesions covering 5-25% of the body. No significant relationships were observed between individual specific growth rates and food consumption rates within the groups. The fish in all salinity groups showed a negative correlation between specific growth rate and food conversion ratio. The coefficient of variation for wet weight specific food consumption and the mean share of meal for each fish were used as a measure of social hierarchy strength. A negative correlation was observed between coefficient of variation in food consumption and mean share of meal. The social hierarchy structure was similar in all salinities; 25% of the fish were dominant (18.29% above an equal share of meal) and 30% were subordinate (16.19% below an equal share of meal) and the remainder 45% fish fed theoretical share of meal (MSM, 5.26%).

Length-weight relationship and relative condition factor in Oreochromis mossambicus (Peters) of Bhima River

Oreochromis mossambicus (Peters), a common freshwater fish of Bhima river, has high economic value and considerable fishery importance. The length-weight relationship in the logarithmic way for this fish can be written as: Log W = - 4.50241627 + 2.884822741 log L. This is close to the cubic law indicating the isometric growth of the fish in its natural habitat. The correlation coefficient (r) was found to be 0.9865 which showed a good relationship between the two parameters. The mean relative condition factor (K sub(n)) was 1.00 suggesting the well-being of the fish.

Microhabitat breadth of fish community along the Vamanapuram River, south Kerala

The microhabitat breadth of Vamanapuram River fish community was studied in detail. The microhabitat variables selected were relative depth, focal point velocity, water column depth, mean water velocity and substrate. Puntius filamentosus had the highest breadth in three dimensions in both stream and river habitats showing a generalistic mode of resource utilization. Garra mullya, Labeo dero and Glossogobius giuris are specialists in the usage of microhabitat variables in the stream habitat while these are G. mullya, Etroplus maculatus and Aplocheilus lineatus in river habitat. Danio aequipinnatus showed extreme variations along focal point velocity variable in both habitats indicating an. opportunistic behaviour.

Benthic diversity of River Gomti in relation to the prevailing environmental conditions in Lucknow

To evaluate the benthic diversity of River Gomti in relation to the prevailing environmental conditions, four stations, viz., Maa Chandrika Devi, Daliganj, Ambedkar Park and Aquaduct, were identified from upstream to downstream along the course of the river in Lucknow. Dissolved oxygen was low on many occasions at all the stations except Maa Chandrika Devi and chemical oxygen demand values were high. There was a gradual increase in mean nitrite and phosphate values from up to downstream. Benthic fauna was dominated by oligochaetes and chironomus larvae. Other groups reported were leeches, nematodes, sponges, crustaceans, pelycypodes, gastropods and fish fry. Population density was exceptionally high at Daliganj (20,135 m sub(-1) ) followed by Ambedkar Park (5,199 m sub(-1)) and Aquaduct (3,287 m sub(-1)), and low at Maa Chandrika Devi (264 m sub(-1)). Oligochaete genera common at all the four stations were Lumbricillus, Limnodrillus, Branchiura, Chaetogaster, Nais and Tubifex. Odonates were reported only from Maa Chandrika Devi while sponges were encountered at Daliganj and Aquaduct. On some occasions, fish fry were also found at Ambedkar Park and Aquaduct. Seasonally, maxima for population density were observed during pre-monsoon and minima during monsoon. The organic pollution indicator benthic species reported were tubificids, chironomids, culicoid larvae, Lamellidens sp., Corbicula sp., Lymnaea sp. and leech. Branchiurans, Tubifex sp. and Chironomus larvae were reported at all the stations. Filthy condition with foul smell throughout the length of the river coupled with poor water quality and appearance of indicator organisms at all the stations indicate that the river is under severe pollution stress due to anthropogenic discharges and it has reached an alarming stage.

Study of fish diversity in Nira River

Fish diversity in Nira River in Pune District has been studied. The study revealed the presence of 24 species of fish belonging to eight orders (Cypriniformes, Siluriformes, Perciformes, Osteoglossiformes, Synbranchiformes, Clupeiformes, Mugiliformes and Aulopiformes). The predominant orders of fishes in this area (Sangavi) are Cypriniformes, Siluriformes and Perciformes. The highest number of ten species was recorded in the order Cypriniformes. The fishes recorded were found to be widely distributed and were present in good numbers in the river.

Morphological overlap of fish community in Vamanapuram River, south Kerala

The morphological characteristics and overlap of fish community in Vamanapuram River have been studied in detail. In the 12 study sites, 19 fish species were encountered. Based on the body shape, four different types are apparent. The elongate bodied fishes (RBD<1.5) like Hemiramphus xanthopterus and Xenentodon cancila are grouped under one category. The deep bodied fishes (RBD>3.5) like Puntius filamentosus, P. ticto, P. vittatus, P. melanampyx, P. sarana, Etroplus maculatus and E. suratensis come under a separate category. Fishes with round to square cross section like Garra mullya and Glossogobius giuris form a separate group. All the other species are grouped as generalized bodied fishes. The morphological overlap studied for the Vamanapuram fish community showed that out of 190 combinations, 30 combinations have high overlaps (≥ 67). P. melanampyx has maximum number (6) of high overlaps. Puntius spp., which constituted 49.5% of the total population, have a mean morphological overlap of 52%. The morphological overlap of fish species in relation to the trophic structure is discussed in detail.