Agiis: a bivalve cultivated as live shrimp feed

Live 'agiis' have been proven to be a good feed for shrimp for the past ten years by polyculture fish farmers in the province of Capiz, in the island of Panay, west central Philippines. A brief account is given of culture and feeding operations. Its cultivation period is short and seeds are readily available. It can reduce dependence on trash fish which is now getting to be scarce; it also seems much cheaper. Perhaps this fast-growing tiny bivalve can be scientifically investigated by students of aquaculture as feed for other commercial aquaculture species. Its scientific identification can be a good start.

Detection of viral diseases in shrimp Litopenaeus vannamei in Iran with emphasis on white spot disease prevention by using marine alga extract

Nowadays, following was expanded shrimp breeding and culture; viral diseases have been main problem which threatened shrimp industry in the country. Therefore, shrimp samples were obtained from different stages of Litopenaeus vannmei life cycle (larval, post larval, juveniles, adults and broodstocks) based on clinical signs in the breeding center and shrimp farming from Bushehr, Khozestan and Sistan and Baluchestan provinces. Viral diseases were detected by PCR (Polymerase Chain Reaction), histopathology and transmission electron microscopy (TEM) methods. Results of the PCR were indicated present white spot virus (WSV) in juveniles, sub adults and adults shrimp with medium intensity from three provinces, but it was not showed in larval and post larval stages. Histopathological sections were indicated hypertrophy and basophilic Cowdry type A formation in nucleus cells of gill, haematopoietic, lymphoid and epithelial's cuticles and intestinal tissues which was associated with small vacuoles increased in B cells of hepatopancreas tissue of infection shrimps. Transmission electronic microscopic studies were demonstrated that the length and diameter virus was detected, respectively, 300 ± 20 nm and 75 ± 5 nm. Considerable, results of the PCR were only displayed IHHNV in juvenile, adult and broodstock shrimps from breeding and farming center of Bushehr province. The main lesion pathology was formed eosinophilic Cowdry type A in nucleus cells of gill, haematopoietic, lymphoid and epithelial's cuticles and intestinal tissues. Whereas penaeid shrimps are lack specific immune system, hence, in the present study was used of marine alga (Lurensia snideria) collected from along costal Persian Gulf of Bushehr province for viral diseases were prevented. Powder alga extract were added with a ratio of 1 % to shrimp diet. Total haemocyte count (THC) and total protein plasma (TPP) were increased after 5 days of oral administration diets. When shrimps were infected by with spot virus experimentally, THC and TPP gradually were increased in both two groups (shrimps fed with diet containing alga extract and without alga extract) after 48h. Nevertheless; THC, TPP and survival of shrimp fed with diet containing alga extract were more than shrimp control in 15 days. So, oral administration Lurensia snideria extract was capable prevention infected L. vannamei via stimulant specific immune system.

Investigation on possibility of survival and growth enhancing by selected seaweeds from Persian Gulf in post larvae shrimp Litopenaeus vannamei

The use of antibiotics in aquaculture has been limited. Scientifics seeking for natural substitutes to prevent of aquatic animals diseases. Considering seaweeds are rich of nutritions and bioactive compounds, the purpose of this study is: investigation the potential and use possibility of native seaweeds from Persian Gulf in shrimp aquculture industry to improve growth, survival of postlarvae and to resistance against pathogens such as vibriosis. For this propose 7 macroalgae species from Bushehr province coast, inclouding: green algae (C. iyengarii), brown algae (S. angutifolium and S. ilicifolium) and red algae (L. snyderiae, K. alvarezii and G. corticata) were collected and identified. Then seaweed extracts abtained by Water, Ethanol, Methanol and Chloroform solvents by soaking method. In vitro antibacterial activity of extracts against Gr+ bacteria (S. aureus and B. subtilis) and Gr- bacteria (V. harveyi, V. alginolyticus and E. coli) was conducted by Agar diffusion, MIC and MBC methods. Antioxidant activity also by DPPH and EC50 methods was investigated. According to results of these two tests four seaweeds species (S. angutifolium, L. snyderiae, K. alvarezii and G. corticata) were selected for use in shrimp postlarvae (PL22) diets by Bio-Encapsulation (Artemia enrichment). Before of enrichment, toxicity effect of extracts to Artemia nauplii were evaluated by determination of LC50 24 h method. From results of this section Ethanol extracts were selected to bioencapsulation. After encapsulation shrimp postlarvae divided to 12 groups in triplicate, namely: C-, C+, S (200), S (400), S (600), L(200), L(400), L(600), G(300), G(600), K(300) and K(600). During 30 days of reared period C- and C+ use of basal diet and unenriched Artemia, but the other groups use of basal diet and enriched Artemia. Except C-, the shrimps in first day of culture put in 107 cfu/ml v. harveyi suspension for 30 minutes, and after water exchange 10 ml of this dose was added to reared aquaria. After 30 days survival percentage, obtained weight and SGR% were investigated. To evaluate vibrio loading, every 10 days 5 postlarvae were sampled randomly for vibrio count. Results showed that vibrio count in C- was less than the others and in C+ was more than the others. In treatments vibrio count in L(200) was the most and L(600) was the less. Survival rate in C- was the most and after that G(600) with 79.4±6.6% and then S(300) and K(600) were 73.3±7.3% and 70.6±6.6% respectively that were significantly compare the other (P < 0.01). Also the C+ was the less with 33.3±6.6% that difference was significant (P< 0.01). In this study growth parameters of all groups that fed by enriched Artemia were better than C+ (P<0.05). After cultre period 10 shrimp of every aquarium disinfected and reared for 10 days like before treatment. After 10 days the shrimps were challenged by 3×108 cfu/ml V. harveyi and mortality was recorded for 7 days. The all of animals in C- were survive but more than 90% of C+ were dead. And survival in all of treatments were better the C+ (P<0.05). The study showed the ethanol extracts of selected seaweed from Persian Gulf is a good source for growth, Survival and disease control in shrimp larviculture.

Protection of white leg shrimp (Litopenaeus vannamei) against white spot disease virus using oral recombinant bioencapsulated VP28

Aquaculture, is perceived as having the greatest potential to meet the growing demand for aquatic food. Crustaceans form one of the main value added components in aquaculture and among them, shrimp aquaculture is the predominant one. Industrial shrimp fanning, in combination with poor management in shrimp aquaculture, has quickly led to severe pollution in shrimp ponds, thereby creating a suitable environment for development of bacterial and virus diseases. White spot disease is one of the most deadly diseases that are caused heavy loss in all Penaeid shrimps family. In Iran during 2002 to 2004 in the Kuzestan province and in 2005 in Bushehr province, the most ponds and farms infected with white spot and the entire industry was facing threat of closure. Owing to the impact of WSSV infection to shrimp aquaculture, there is an urgent need to develop suitable strategies to protect cultured shrimps and make aquaculture more sustainable. Therefore, this study aimed to examine the possibility of protecting shrimp against white spot syndrome virus using bioencapsulated Anemia with E. coil containing the recombinant protein VP28, designed. Virus genome was extracted from naturally infected Litopenaeus vannamei in the Choebdch farms and VP28 gene by designed primers was amplified, extracted, purified and cloned in E. coli TGI. Protein expression evaluated and inactivated bacteria containing recombinant protein encapsulated in Artemia nauplii. White shrimp post larvae stage 5 were fed for 5 days with recombinant nauplii and twice on days 7 and 25 after feeding with Artemia nauplii were challenged with white spot virus. The results of the first experiment revealed that cumulative mortality percent in the group receiving the bacteria containing recombinant plasmid (pMal + VP28) was %14.44±1.11 and the relative percent survival %80.30±1.51. In this group the mortality rates in the various repetitions varied from the 13.33% to 16.66% and relative percent survival of 77.27% to 81.81%. in the Non-recombinant plasmid group (pMal) Mean percent mortality was% 33.33±3.84 and the Relative Percent Survival %54.54±5.24 and in the group that received bacteria contained no recombinant plasmid the Mean cumulative mortality percent was%48.88 ± 5.87 and Relative Percent Survival%33.33± 8.01.

Aquaculture Asia, Vol.13, No.1, pp.1-56, January-March 2008

Developing guidelines for sustainable freshwater aquaculture planning in Vietnam. Aquaculture production, certification and trade: Challenges and opportunities for the small-scale farmer in Asia. The successful development of backyard hatcheries for crustaceans in Thailand. Alternate carp species for diversification in freshwater aquaculture in India. Genetic and reproduction technologies for enhanced aquaculture and fisheries management of Murray cod. Effluent and disease management in traditional practices of shrimp farming: A case study on the west coast of Sabah, Malaysia. Status of sahar domestication and its development in the Himalayan Region of Nepal. Vaccination benefits highlighted as Schering-Plough reinforces commitment to Asian aquaculture. Comparative advantage analysis of shrimp production in Asia. Strategies to improve livelihood of the rural poor: A case study in two small reservoirs in Binh Phuoc Province, Vietnam. Cambodian Government ban on snakehead farming enforced. Marine finfish aquaculture developments at ‘Indonesian Aquaculture 2007’. Production update – marine finfish aquaculture in the Asia-Pacific region. Body size of rotifers from estuaries in North Sulawesi. NACA Newsletter.

Aquaculture Asia, Vol. 10, No. 4, pp.1-42, October-December 2005

*Table of Contents* Sustainable aquaculture Peter Edwards writes on rural aquaculture: Small-scale pond culture in Bangladesh. People in aquaculture Community based aquaculture - issues and challenges H.K. De and G.S. Saha. Aquaculture as an action programme: An exercise in building confidence and self worth. B. Shanthi, V.S. Chandrasekaran, M. Kailasam, M. Muralidar, T. Ravisankar,.C. Saradad and M. Krishnan The STREAM Column: Transforming policy recommendations into pro-poor service provision Graham Haylor. Research & farming techniques. Grow out of juvenile spotted Babylon to marketable size in earthen ponds II: Polyculture with seabass. S. Kritsanapuntu, N. Chaitanawisuti, W. Santhaweesuk and Y. Natsukari Asia-Pacific Marine Finfish Aquaculture Network. Influence of economic conditions of importing nations and unforeseen global events on grouper markets. Sih Yang Sim. Present status of hatchery technology for cobia in Vietnam. Nhu Van Can. Report on grouper hatchery training course in Indonesia. Nguyen Quoc Thai. Aquatic animal health. Biosecured and improved penaeid shrimp production through organic nursery raceway system in India. Felix. S. and M. Samaya Kannan. Management of monogenean parasites in brackishwater finfish. K.P. Jithendran, M. Natarajan and I.S. Azad. Vembanad Lake: A potential spawner bank of the giant freshwater prawn Macrobrachium rosenbergii on the southwest coast of India. Paramaraj Balamurugan, Pitchaimuthu Mariappan & Chellam Balasundaram.

Aquaculture Asia, Vol. 8, No. 2, pp.1-47, April-June 2003

*Table of Contents* Sustainable Aquaculture Peter Edwards writes on rural aquaculture: Peri-urban aquaculture in Kolkata A case of informal shrimp farmers association and its role in sustainable shrimp farming in Tamil Nadu, India M. Kumaran, N. Kalaimani, K. Ponnusamy, V.S. Chandrasekaran, D. Deboral Vimala Diffusion and adoption of shrimp farming technologies M. Kumaran, K. Ponnusamy and N. Kalaimani Farmers as Scientists: Aquaculture education in India - opportunities for global partnership M.C. Nandeesha Information system of fish germplasm resources in China Yang Ningsheng, Ge Chanshui, Ouyang Haiying, Yuan Yongming Status and development needs of freshwater crustacean aquaculture in China Xu Pao Research and Farming Techniques Aquaculture fundamentals: Getting the most out of your feed Part II: The role of macronutrients Simon Wilkinson Fish breeding in captivity - some innovative adaptations of technology by Bengal farmers N.R. Chattopadhyay Scientific guidelines for farmers engaged in freshwater prawn farming in India Vishal Saxena Marine Finfish Section News and publications Status and development of mariculture in Indonesia Ketut Sugama Aquatic Animal Health Use of probiotics in larval rearing of new candidate species Rehana Abidi Advice on aquatic animal health care: Problems in shrimp culture during the wet season (Thai/English languages) Pornlerd Chanratchakool

Marine protected areas and artisanal fisheries in Brazil

Marine protected areas (MPAs) are being promoted around the world as an effective means of protecting marine and coastal resources and biodiversity. However, concerns have been raised about their impact on the livelihoods, culture and survival of small-scale and traditional fishing and coastal communities. Yet, as this study from Brazil shows, it is possible to use MPAs as a tool for livelihood-sensitive conservation. Based on detailed studies of three sites–the Peixe Lagoon National Park in Rio Grande do Sul, and the marine extractive reserves (MERs) of Mandira, São Paulo, and Corumbau, Bahia – the study shows how communities in Brazil have been able to use protected areas to safeguard their livelihoods against development and industrialization projects, like shrimp farms and tourist resorts. (68 pp.)

STREAM Journal, Vol. 1, No. 1, pp. 1-12. January-March 2002

CONTENTS: Learning from each other about conflict, by Ronet Santos. E-learning to support knowledge sharing in aquatic resources, by Robert T. Raab and Jonathan Woods. Livelihood strategies, gender and participation in aquaculture: findings from participatory research in northwestern Sri Lanka, by Lindsay J. Pollock and David C. Little. Farming of giant tiger shrimp in northern central Vietnam, by Jesper Clausen. Interacting with stakeholders and policy-makers, by To Phuc Tuong. The role of nutrition communications in meeting the nutritional challenges of the Asia-Pacific region, by Georgina Cairns. (PDF has 16 pages.)

STREAM Journal, Vol. 3, No. 1, pp 1-18. January-March 2004

CONTENTS: Creating understanding and ownership of collaborative research results through ‘learning by doing,’ by Robert Arthur and Caroline Garaway. Fish culture, farming, markets and promotion: an integrated, sustainable approach to aquaculture and rural development, by Pen Rotha and Brendan Boucher. Fisheries policy reform impact assessment in Cambodia: understanding policy and poor people, by Philip Townsley and Sem Viryak. “Shrimp Hero” Phan The Phuong, by Ngo Minh Khoi. Coral farming in Vietnam, by Nguyen Viet Vinh. The global fisheries market: can rural poor people benefit? Issues raised by STREAM Media Monitoring Reports, by Paul Bulcock.

STREAM Journal, Vol. 5, No. 4, pp 1-18. October-December 2006

CONTENTS: Success story of one-stop aqua shop in Kaipara Village, by Kuddus Ansary. Enhancement of people’s livelihoods in Kompong Kra Sang community fishery, by Chun Sophat. Char livelihoods of the old Brahmaputra River in Bangladesh, by Nesar Ahmed. Fin fish community structure as a measure of ecological degradation in two tropical rivers of India, by D. Chakrabarty and S.K. Das. Penaeid shrimp fisheries of Pakistan, by Razia Sultana. Reviving the shrimp industry in Capiz, by Jessica C. Esmao.

Forecasting Climate Impacts on Future Production of Commercially Exploited Fish and Shellfish

Foreword [pdf, < 0.1 MB] Acknowledgements PHASE 1 [pdf, 0.2 MB] Summary of the PICES/NPRB Workshop on Forecasting Climate Impacts on Future Production of Commercially Exploited Fish and Shellfish (July 19–20, 2007, Seattle, U.S.A.) Background Links to Other Programs Workshop Format Session I. Status of climate change scenarios in the PICES region Session II. What are the expected impacts of climate change on regional oceanography and what are some scenarios for these drivers for the next 10 years? Session III. Recruitment forecasting Session IV. What models are out there? How is climate linked to the model? Session V. Assumptions regarding future fishing scenarios and enhancement activities Session VI Where do we go from here? References Appendix 1.1 List of Participants PHASE 2 [pdf, 0.7 MB] Summary of the PICES/NPRB Workshop on Forecasting Climate Impacts on Future Production of Commercially Exploited Fish and Shellfish (October 30, 2007, Victoria, Canada) Background Workshop Agenda Forecast Feasibility Format of Information Modeling Approaches Coupled bio-physical models Stock assessment projection models Comparative approaches Similarities in Data Requests Opportunities for Coordination with Other PICES Groups and International Efforts BACKGROUND REPORTS PREPARED FOR THE PHASE 2 WORKSHOP Northern California Current (U.S.) groundfish production by Melissa Haltuch Changes in sablefish (Anoplopoma fimbria) recruitment in relation to oceanographic conditions by Michael J. Schirripa Northern California Current (British Columbia) Pacific cod (Gadus macrocephalus) production by Caihong Fu and Richard Beamish Northern California Current (British Columbia) sablefish (Anoplopoma fimbria) production by Richard Beamish Northern California Current (British Columbia) pink (Oncorhynchus gorbuscha) and chum (O. keta) salmon production by Richard Beamish Northern California Current (British Columbia) ocean shrimp (Pandalus jordani) production by Caihong Fu Alaska salmon production by Anne Hollowed U.S. walleye pollock (Theragra chalcogramma) production in the eastern Bering Sea and Gulf of Alaska by Kevin Bailey and Anne Hollowed U.S. groundfish production in the eastern Bering Sea by Tom Wilderbuer U.S. crab production in the eastern Bering Sea by Gordon H. Kruse Forecasting Japanese commercially exploited species by Shin-ichi Ito, Kazuaki Tadokoro and Yasuhiro Yamanka Russian fish production in the Japan/East Sea by Yury Zuenko, Vladimir Nuzhdin and Natalia Dolganova Chum salmon (Oncorhynchus keta) production in Korea by Sukyung Kang, Suam Kim and Hyunju Seo Jack mackerel (Trachurus japonicus) production in Korea by Jae Bong Lee and Chang-Ik Zhang Chub mackerel (Scomber japonicus) production in Korea by Jae Bong Lee, Sukyung Kang, Suam Kim, Chang-Ik Zhang and Jin Yeong Kim References Appendix 2.1 List of Participants PHASE 3 [pdf, < 0.1 MB] Summary of the PICES Workshop on Linking Global Climate Model Output to (a) Trends in Commercial Species Productivity and (b) Changes in Broader Biological Communities in the World’s Oceans (May 18, 2008, Gijón, Spain) Appendix 3.1 List of Participants Appendix 3.2 Workshop Agenda (Document contains 101 pages)

Aquaculture Asia, Vol.13, No.4, pp.1-51, October-December 2008

Editorial An increasingly secure future for wastewater-fed aquaculture in Kolkata, India? by Peter Edwards. First culture-based fisheries growth cycle in Lao PDR is overwhelmingly encouraging, by Sena De Silva. Revival of abandoned shrimp farms in Krishna District of Andhra Pradesh, by National Centre for Sustainable Aquaculture (NaCSA). Growth of forward and backward industries linked with aquaculture in Kolleru Lake area, Andhra Pradesh, India, by A. K. Roy, G. S. Saha, P. Kumaraiah and N. Sarangi Effective marketing strategies for economic viability of prawn farming in Kuttanad, India, by Ranjeet K. and B. Madhusoodana Kurup. Applications of nutritional biotechnology in aquaculture, by S.D. Singh, S.K. Nayak, M. Sekar and B.K. Behera. Some technical and management aspects of catfish hatcheries in Hong Ngu district, Dong Thap province, Vietnam, by H. P. Hung, N. T. T. An, N. V. Trieu, D. T. Yen, U. Na-Nakorn, Thuy T. T. Nguyen. Nodavirus: An emerging threat to freshwater prawn farming, by Biju Sam Kamalam, J., Saravanan, S. and Ajith Stalin, J.L. Asia-Pacific Marine Finfish Aquaculture Network Magazine: Asian seabass farming: Brainstorming workshop and training in India. Comparative study for broodstock management of grey mullet (Mugil cephalus L.) in cages and earthen ponds with hormone treatment, by Nani Gopal Das, Md. Shahadat Hossain, Sushanta Bhattacharjee and Prabal Barua. Cultivation of gilthead sea bream (Sparus auratus L.) in low saline inland water of the southern part of Israel desert, by Samuel Appelbaum and A. Jesu Arockia Raj. Mariculture development opportunities in SE Sulawesi, Indonesia, by La Ode M. Aslan, Hotman Hutauruk, Armen Zulham,Irwan Effendy, Mhummaed Atid, Michael Phillips, Lars Olsen, Brendan Larkin, Sena S De Silva, Geoff Gooley. Improved hatchery and grow-out technology for marine finfish. NACA Newsletter

Mercury in Florida Bay fish: spatial distribution of elevated concentrations and possible linkages to Everglades restoration

Health advisories are now posted in northern Florida Bay, adjacent to the Everglades, warning of high mercury concentrations in some species of gamefish. Highest concentrations of mercury in both forage fish and gamefish have been measured in the northeastern corner of Florida Bay, adjacent to the dominant freshwater inflows from the Everglades. Thirty percent of spotted seatrout (Cynoscion nebulosus Cuvier, 1830) analyzed exceeded Florida’s no consumption level of 1.5 μg g−1 mercury in this area. We hypothesized that freshwater draining the Everglades served as the major source of methylmercury entering the food web supporting gamefish. A lack of correlation between mercury concentrations and salinity did not support this hypothesis, although enhanced bioavailability of methylmercury is possible as freshwater is diluted with estuarine water. Stable isotopes of carbon, nitrogen, and sulfur were measured in fish to elucidate the shared pathways of methylmercury and nutrient elements through the food web. These data support a benthic source of both methylmercury and nutrient elements to gamefish within the eastern bay, as opposed to a dominant watershed source. Ecological characteristics of the eastern bay, including active redox cycling in near-surface sediments without excessive sulfide production are hypothesized to promote methylmercury formation and bioaccumulation in the benthos. Methylmercury may then accumulate in gamefish through a food web supported by benthic microalgae, detritus, pink shrimp (Farfantepenaeus duorarum Burkenroad, 1939), and other epibenthic feeders. Uncertainty remains as to the relative importance of watershed imports of methylmercury from the Everglades and in situ production in the bay, an uncertainty that needs resolution if the effects of Everglades restoration on mercury levels in fish are to be modeled and managed.