HACCP based management system for improving quality of freshwater giant prawn, Macrobrachium rosenbergii

The study was conducted on the present status of HACCP based quality management system of golda, Macrobrachium rosenbergii farms in Fulpur region of Mymensingh. Information was collected on general condition of farms, culture systems and post-harvest quality management. In almost all farms, there is no or inadequate infrastructure facilities such as, road access, electric supply, telecommunications, ice, feed storage facility, vehicle for golda transportation, washing and toilet facilities. The problems associated with sanitation and hygiene was: widespread use of cow dung, poultry manure and construction of open toilet within the vicinity of prawn culture pond. Different grades of commercially available and locally prepared feeds were used for golda culture in the pond. Golda post-larvae (PL) of 40-50 days old were stocked with carp species. The price of golda PL ranged from Tk. 1.00 to Tk. 1.25/piece. The pond size varied from 50 decimal (0.2 ha) to 2.5 acre (1.0 ha) with an average depth of 2-2.5 m. The culture period of golda varied from April-May to November-December and survival rate ranged between 75 and 80%. Production of golda varied from 250-500 kg/acre (625-1,250 kg/ha). Harvested golda were transported to city market within 4 h. Two size grading were generally followed during pricing, e.g. Tk. 500 to 550/kg for >100 g size and Tk. 300/kg for <100 g size. The cost-benefit ratio was found to remain around 1:1.25 depending on availability of PL. Water quality parameters such as, water temperature, pH, dissolved oxygen, total alkalinity and chlorophyll a in five golda farms in Fulpur region were monitored. Water temperature ranged from 29°C to 33°C, dissolved oxygen from 2.28 to 4.13 mg/l, pH between 6.65 and 7.94, alkalinity from 44 to 70 mg/l and chlorophyll a concentration from 61.88 to 102.34 µg/l in the five investigated ponds. The Aerobic Plate Count (APC) of the water sample was within the range of 2.0x10^6 - 2.96x10^7 CFU/ml and of soil samples within the range of 6.9x10^6 - 7.73x10^6 CFU/g. Streptococcus sp., Bacillus sp., Escherichia coli, Staphylococcus sp., Pseudomonas sp. and Salmonella sp. were isolated from pond water and sediment. Different feed samples used for golda was analyzed for proximate composition. Moisture content ranged around 14.14-21.22%, crude protein 20.55-44.1%, lipid 4.67-12.54% and ash 9.7-27.69%. The TVB-N values and peroxide values of feeds used as starter, grower and fish meal were found within the acceptable ranges and samples were free from pathogenic organisms. A training was organized for the golda farmers on HACCP, water quality and post-harvest quality management of prawn.

Demonstration of some selected aquaculture technologies under farming system research in Jessore and Santahar, Bangladesh

BFRI evolved some selected aquaculture technologies viz. polyculture of carps in perennial ponds, monoculture of short cycled fish species (BFRI super strain) in seasonal ponds and prawn seed production through backyard hatchery system have been demonstrated under Farming System Research (FSR) component in Jessore and Santahar regions. Both polyculture of carps and monoculture of short cycled fish species technologies were tested in farmer's ponds in Kaium Kula village near Jessore town. In polyculture trials, seven species comprising of silver carp (Hypophthalmichthys molirrix), catla (Catla catla), rohu (Labeo rohita), grass carp (Ctenopharyngodon idellus), common carp (Cyprinus carpio), mrigal (Cirrhinus cirrhosus) and silver barb (Barbonymus gonionotus) were stocked @ 9,500 (ratio 6:2:4:2:1:5:5); 10,750 (ratio 6:2:4:2:1:5:5) and 12,000 (ratio 6:2:4:2:1:5:4) fish/ha respectively in ponds of T1, T2 and T3 having three replications of each. The mean highest fish production was 3,148 kg/ha in T3, followed by 2,899 kg/ha in T1 and 2,875 kg/ha in T2. Production of T3 was significantly different (P<0.05) than both T1 and T2, while there was no significant differences (P>0.05) between the production of T1 and T2. In case of trial of short cycled fish species, two treatments were tested: T1 (comprising of BFRI super strain of Nile tilapia, silver carp, common carp and silver barb; ratio 3:5:1:1) and T2 (having only BFRI super strain of Nile tilapia). Stocking density in both the treatments were same (20,000 fish/ha). In this trial average production was higher in T1 (2,743 kg/ha) than that of T2 (2,369 kg/ha) but the production figure in these two treatments was not significantly different (P>0.05). Demonstration of backyard prawn hatchery technology was tested at Santahar region of Bogra district, North-west part of Bangladesh. This hatchery consisted of three main components i) bio-filter, ii) rearing tank unit (chari) and iii) air blower/air pump unit. Plastic drum of 200-250 l capacity and cemented chari of 200-250 l capacity were used as bio-filter and larval rearing containers respectively. A 0.5 hp air blower with 6 aquarium air pump were used to operate the aeration system in the hatchery. Diluted sea water (10-12 ppt) made from brine solution (200-250 ppt) collected from salt-bed was used in the backyard hatchery system of hatching of eggs and rearing of larvae. Rearing of first stage zoea-larvae was reared in three rearing tanks following the stocking densities of 40, 50 and 60/l of water respectively. Production of post-larvae were 20±0.82, 22±1.12 and 28±1.63/liter of water in treatments I, II and III respectively in 38, 40 and 39 days rearing period.

Observations on the ingression and distribution of larval prawns in the Nethravati-Gurupur estuary, Mangalore

The spatial and temporal distributions of larval prawns of penaeids and non-penaeids in the estuarine waters of Mangalore were studied. Larvae appear to be passively brought in by the incoming flood tides to the estuary, enjoy a wider distribution throughout the estuarine complex with abundance towards the mouth. The distribution of larval prawns was more in Nethravati than in the Gurupur stretch. The influence of temperature, hydrogen-ion-concentration, salinity and dissolved oxygen on the distribution of larvae in the estuaries is discussed. Inference on spawning seasons of commercially important prawns in the neighbouring waters has been arrived at based on their larval abundance.

Experiments on the induced spawning and larval rearing of the milkfish, Chanos chanos (Forsskal) in 1979

This paper summarizes the results of the experiments on the induced breeding and larval rearing of milkfish (Chanos chanos) during the 1979 season. Milkfish larvae could be reared successfully without the use of trochophore larvae of oysters as feed during the first few days. In order to induce the ovulation of wild adult milkfish a higher dose of human chorionic gonadotropin hormone is required.

Identification and culture of common diatoms as possible feed for Penaeus monodon

Diatoms were collected from Buyuan Bay, and from the hatchery tanks at Tigbauan, to determine the commonly occurring species, the feasibility of culturing these species, and the potential of these selected species as food for larval P. monodon. The commonly occurring diatoms were identified as Chaetoceros calcitrans, Navicula grimmei, Nitzchia seriata, Nitzchia closterium and Amphiprora sp. These diatoms were isolated and unialgal cultures prepared. Protein content analysis using the micro-Kjildahl method gave the following result: C. calcitrans, 11 . 78%; Nitzchia seriata, 25%; Nitzchia closterium, 30 . 5%; Navicula grimmei, 9 . 06% and Amphiprora sp. 8 . 96%. Feeding experiments were conducted to determine acceptability of the different diatom species and percentage survival of larval stages Z SUB-1 -M SUB-2 . Larvae were placed in 4-l capacity plastic containers with a stocking density of 10/l. The results of several feeding trials using the different mass-produced diatoms are summarized. From the data gathered, C. calcitrans appears to be the most promising candidate as feed for zoea and mysis stages of P. monodon. The average percentage survival of C. calcitrans was 63 . 76% for the 3 trials, and as high as 82 . 22% in the third trial. Comparatively high percentage survival of larvae was also recorded when Nitzchia seriata (48 . 17%) and Nitzchia closterium (67 . 6%) were given as feed, while both Amphiprora sp. and Navicula grimmei gave 0% survival. The poor results with Amphiprora sp. and Navicula grimmei may be due to their low protein content (8 . 96% and 9 . 06%, respectively) and the inability of the larvae to ingest them. Navicula and Amphiprora were observed to cling to the appendages of the larvae and to settle down in the medium making them unavailable to the larvae. Low survival was also noted when frozen C. calcitrans was used (14 . 25%). This may be due partly to the effect of the floculating agent (ALSO SUB-4 . 25 g/l) used in concentrating the diatoms. When protein contents of C. calcitrans, N. seriata and N. closterium are compared, the 2 Nitzchia species have relatively higher protein contents than C. calcitrans and, therefore, could be the more desirable feed candidates. However, few feeding trials were made using Nitzchia so that additional investigations will have to be done on this aspect.

Effect of furanace on the development of larval stages of Penaeus monodon Fabricius

Zoea 2(Z SUB-2 ) Mysis 1 (M SUB-1 ) and Postlarva 1 (P SUB-1 ) of P. monodon artificially spawned in closed-system concrete hatchery tanks were bioassayed for their tolerance to the antibiotic furanace. The setup consisted of four 20-liter capacity plastic basins previously conditioned for 15 days with freshwater in full sunlight. During the experiment, each basin was filled with 5 liters of seawater to which was added filtered Chaetoceros and Brachionus to give densities of 5 . 0-7 . 5 x 10 SUP-4 cells/ml and 10-20 individuals/ml, respectively. The following are the properties of the water used throughout the experiments: salinity, 26-32%; pH, 7 . 3-8 . 4; temperature, 25-30 degree C; dissolved oxygen, 4 . 5-8 . 4 ppm; nitrite, 0 . 36-0 . 99 ppm; and ammonia, 0 . 10-0 . 30 ppm. To each basin were added 50 healthy larvae of specific stages of P. monodon. After an initial acclimation of one hour in the medium, preweighed amounts of the antibiotic were added and thoroughly dissolved. The concentrations tested were 1 . 0, 2 . 0 and 3 . 0 ppm. One basin always served as control. After 24 hours of exposure, the surviving population in each basin was counted. The survivors were then examined thoroughly under the microscope for unusual behavior and morphological defects brought about by the exposure. To minimize wide variations in the medium as a result of feeding and other manipulations, the systems were all prepared at 9:00 a.m. each time, and the feeds on two instances, one at 5:00 p.m. and another at 5:00 a.m. Fifteen trials conducted with Z SUB-2 showed survival ranges of 68% to 98% with a mean of 77 . 6% in the controls; 32% to 94% with a mean of 65 . 7% at 1 ppm, and 0% to 56% with a mean of 36 . 5% at 2 ppm. There were no survivors at 3 ppm. Interpolation from the survival-dose curve gave a 24-hr LC SUB-50 of approximately 1 . 6 ppm.

Effects of furanace on Brachionus

Tiger prawn P.monodon) larvae utilize Brachionus a rotifer, as food in the Zoea 3 and mysis stages when they change from an herbivorous to an omnivorous diet. The present work aims to show the effects of furanace on the population growth of Brachionus. Cultures of Brachionus were obtained and fed with Chlorella at a density of 1-2x10 SUP-6 cells/ml. Five liters of the culture water were placed in each of 4 white, circular, 152x304 mm plastic basins. The mean initial densities of the rotifer ranged from 26 . 5 to 38 . 5 individuals/ml. The concentrations of furanace were 0, 1, 2 and 3 mg /l. The cultures were vigorously aerated. Population growth was observed after 3, 6, and 9 hours of exposure. The cultures were thoroughly mixed before samples were taken to ensure an almost equal distribution of the rotifers in the water. To facilitate the counting of the rotifer, one drop of Lugol's solution was added to each sample. This immobilizes the rotifer as well as stops further reproduction. Individuals with only the lorica left or with badly deformed lorica were considered dead. Population counts were done using a Sedgwick-Rafter counting chamber. Among the different durations of exposure, the percentage survival of the populations in the furanace baths were highest after 3 hr. There were slight increases in the control and 2 mg/l and slight decreases in 1 and 3 mg/l. The differences in the mean densities are statistically insignificant at . 01 significance level. After a 6-hr exposure, the control population reached its peak density with a survival of 89%. Populations in furanace baths decreased to 88 . 5% in both 2 and 3 mg /l followed closely by 87% in 1 mg/l. Again, no statistical differences exist among all the levels. The mean percentage survival in 1 and 2 mg/l increased (89% and 91%, respectively) after a 9-hr expsoure, while those in the control and 3 mg/l decreased to 86 . 5% and 88 . 25%, respectively. There were no marked differences in appearance noted among the individuals in furanace baths and those in the control.

Preliminary biological evaluation of some formulated feeds for P. monodon

Rice bran is widely used by fish farmers as supplementary feed while soybean cake is used both as feed and as fertilizer in fishponds. Both fish meal and shrimp head have been found acceptable as feed ingredients. However, not much is known of the acceptability and efficiency of a mixture of these ingredients as feed for Penaeus monodon larvae. Ninety 127-day old P. monodon were measured for length and weight and were randomly divided into nine aquaria each containing 20 liters of water. These were fed 'lampirong' for two months previous to the study. There were three replications for each treatment. Length, weight, and survival rates were used to compare the efficiency of the diets. Weighed amounts of pellets equivalent to 100% of the body weight were fed during the first three days and reduced to 50% thereafter. A stopwatch was used to determine the length of time that elapsed before the shrimps would approach the pellet. Ten shrimps approximately 4 months in age were placed in 10 liters of water in a 25-liter aquarium. Two grams of each pellet type were placed simultaneously on opposite sides of the aquarium. The time that elapsed from the moment the pellets sunk to the bottom up to the time that any one shrimp approached the pellets was recorded. The group fed the imported pellets gained the most. Those fed FP-2s-77 elongated faster than those fed FP-1s-77. Survival rate of those fed FP-2s-77 was 37% while those fed imported pellets was 73%. Both 1s and 2s pellets disintegrated in water easily but the imported pellets were stable even after six hours in water. The attractability test for the pellets showed that the prawns were more readily attracted to the pellets 1s and 2s than to the imported pellets.

Biology and farming of the green mussel Mytilus smaragdinus

Biological investigations were carried out in Sapian Bay, Capiz from November 1975 to December 1976 with samplings conducted fortnightly. Histological studies on the gonad reveal a high percentage of ripe and spent females during the month of April and May, and ripe to near ripe during November to December. However, larval counts were highest on February 25, 1976 with 253 mytilid larvae per haul compared to 0-79 per haul during all other months. The high larval count was followed by the highest spat settlement during the next sampling period two weeks later, with the spat collector set in the water during the February 25 sampling. The four materials tested, blue polypropylene fiber rope, black polypropylene fiber, and coir rope, all had their highest spat counts during this period with an average of 471 spats per standard 10 cm rope piece. The range during the other time periods is 2-283 spats. Of the 4 materials tested, the black fibrillated polypropylene film had the highest larval counts in 15 out of a total of 25 sampling periods. The blue rope was the poorest spat collector. Coconut husk was tested later on and it proved to have a very high catchability, with spats completely enveloping the husk surface. Growth monitored from one cohort in Sapian Bay averaged 10 mm per month. 50-60 mm is considered marketable size. Trial growth experiments with transplanted mussels were also conducted at Igang Bay in Guimaras Island, Makato River in Aklan, and a milkfish pond in Leganes, Iloilo. Survival in Igang was less than 50% after the second week, and the condition of the surviving mussels can be described only as 'watery' with the mantle completely transparent. Mortality was minimal in Makato but the growth rate was only 30% that of Sapian Bay. The pond experiments were terminated due to severe crab predation.

The propagation of the mud crab Scylla serrata (F.) de Haan

The mud crab Scylla serrata is an important commercial species found in many brackish areas in the Philippines. During spawning and hatching, the berried females migrate to the sea. Seeds for pond stocking are obtained from the wild. Because of the unpredictability of seed supply, there is a need to propagate the species artificially. Thus, spawning, larval rearing, maturation, and rematuration of the species are being studied. The first attempts at hatching S. serrata were successful with rates varying between 75% and 90%. Two out of three trials on larval rearing yielded a few megalops. The first zoeal stages were fed diatoms, rotifers, Artemia salina, and bread yeast. Overfeeding programs were implemented during the critical premolting periods to prevent weakening of the larvae and lessen cannibalism. Larval weakening during the premolt makes them susceptible to attacks by fungi like Lagenidium and ciliates like Vorticella. S. serrata larvae survived salinity levels as low as 15 ppt until the 14th day of rearing. Other larvae were able to survive in salinities of 30-32 ppt for 8 to 13 days. Zoeal molting was hastened by lowering the salinity to 25-27 ppt. Artificial broodstocking of juveniles and adult crabs has been made possible using a simple refuge system made of three-compartmented hollow blocks. This system has been helpful in minimizing fighting among crabs. Remarkable growth rates have been observed with feeds like mussel meat and trash fish. Average growth increments of 11 mm carapace length and 20 . 35 g body weight have been observed every fortnight. A newly spent spawner could gain additional weight of 22 . 5 g in only 6 days. Feeding rates of juveniles and adult crabs have been established based on the average body weight from an experiment using mussel meat. Crabs feed more at night. In another experiment, eyestalk ablation was found to be effective in inducing growth and mating. Aside from hastening the molting process, copulation is induced even among the small crabs (average carapace length = 55 mm). Natural mating lasts about 26 hr. A copulation which lasted for seven days with a break in between was observed.

Notes on the induced maturation and spawning in four-month-old Penaeus monodon Fabricius by eyestalk ablation

The ablation technique consisted of making an incision across the eyeball to allow free flow of fluids while holding the prawn under water, squeezing the eyeball contents outwards, and pinching hard the eyestalk tissue. The cut area heals completely in about a week; no application of antibiotics is necessary. Spent spawners were tagged with thin brass rings (Rodriguez, 1976) around the unablated eyestalk for a separate experiment on rematuration. Two spawning yielding approximately 277,000 eggs were obtained three weeks after ablation, followed four days later by two more spawnings with 160,000 eggs; all four spawners weighed more than 100 g. With a hatching rate of 98% and 78% for the first and second batch, respectively, the spawnings produced viable nauplii. Water temperatures as low as 23 degree C due to a delayed cold spell in March depressed molting; weakened larvae had to be discharged at the mysis stage. Although ovarian development continued, no further spawnings were obtained due mainly to the onset of bacterial and fungal disease. Infection is initiated in injured portions of the exoskeleton, sometimes penetrating right through the muscles to the ovarian tissues. The non-flowthrough conditions and mussel meat feeding led to fouling of the culture water resulting in consecutive mortalities caused by disease. Female P.monodon held in maturation pens were ablated at the age of 15 months (Santiago, et al., 1976); they averaged only 16 g body weight after four months growth in ponds. In another experiment, pond-reared P.monodon females ranging from 50 to 80 g were ablated at approximately seven months (Aquacop, 1977). The present results show a minimum age of four months from postlarve that P.monodon is capable of ovarian development and spawning upon ablation. However, maturation is probably affected by size as well as age - the four-month old females weighed an average of 100 g in contrast to the smaller animals in the earlier experiments.

Land-based mass production of prawn (Penaeus monodon Fabricius) spawners

The first spawnings were obtained 12 days after ablation with 4 spawners yielding 784,000 eggs and a harvest of 250,000 P SUB-10 fry. Survival of females after 1 month was approximately 30%. Mortalities were mostly due to handling stress during the regular ovarian samplings as well as disease frm the accumulated excess feeds on the bottom of the tank. Male survival could not be recorded because of transfers to other tanks and addition of new stocks. Development seemed to peak 3 weeks after ablation. The average number of eggs per ablated spawner was 120,000. However, many of the partially spawned females were removed from the spawning tanks the following day so that remaining eggs released in the next 2 to 3 days could not be recorded. Estimate of the average number of eggs per ablated spawner is 120,000-150,000 in contrast to 500,000 per wild spawner. However, the low production cost more than compensates for the difference. Fry reared in the Wet Laboratory were used for experiments, mostly on feeding. Therefore, survival at harvest is not to be taken as a reflection of stock quality. Although fewer in number, larvae from ablated prawns are as healthy in terms of vigor in swimming and feeding as those from wild females. Most mortalities are due to inability to molt caused by lower water temperatures and inadequate feeding.

Methods of harvesting and preservation of the diatom Chaetoceros calcitrans

Diatom culture and larval feeding experiments were conducted to test the viability and acceptability of preserved algal concentrates. C. calcitrans is characterised by the presence of setae which keep them suspended in cultures and make autoflocculation very difficult. Flocculation was induced by the addition of a floc-forming chemical. Using the optimum conditions, it was possible to harvest the algae within 1-h settling time and with about 84% recovery. The viability of frozen Chaetoceros was determined by actual cell reproduction. Preliminary feeding experiments showed that Chaetoceros can be successfully used as a substitute for fresh diatoms as feed for Penaeus monodon larvae. Simple freezing techniques, with or without the use of protectants has been found convenient for preserving algal concentrates in small volumes for both feeding and culture purposes.

A preliminary study on the use of the local variety of ipil-ipil Leucaena leucocephala as a protein source for prawn feed

A study was carried out to determine the effect of 10 or 20% leaves or seeds in the diet of Penaeus monodon , and the extent to which local ipil-ipil (Leucaena leucocephala ) could replace head shrimp meal. A brief description is given of the experimental methodology, and details of composition of the diet, proximate chemical composition of the diets, mean weight gain and survival of Penaeus monodon larvae fed shrimp head meal and ipil-ipil as protein sources, are presented. Mean weight gains for all groups were poor and not statistically significant. Survival rates for those fed 10% ipil-ipil were significantly higher than those fed 20% diets. Wherever the survival rate was high, mean weight gain was low and vice versa. The presence of the toxic alkaloids mimosine in ipil-ipil could have caused the low survival rate.

Relationship between diet composition and growth rate of the zoeal and mysis stages of Penaeus japonicus Bate

Diets containing Chaetoceros gracilis plus Artemia nauplii artificially prepared diet, Diet-B, and two commercial feeds Tapes and mysid meals, were fed to larvae of P. japonicus. Highest survival rate was obtained when larvae were fed with Diet-B. The results show that the early larval stages of P. japonicus can be reared on artificially prepared diets. Since the chemical composition of the diet is known, it can be used as supplemental data for larval feeding development and nutritional requirement studies for the early larval stages of Penaeus japonicus and/or other penaeids. Information is tabulated on feeds and feeding rates used, composition of the artificial diet, fatty acid composition of lipids of the different diets, and of the sterols of the different diets.