Arctic charr from Ennerdale Water: a pilot study

There are three fish species in the north west of England, Arctic charr (Salvelinus alpinus, L.), schelly (Coreqonus lavaretus. L.), and vendace (C. albula, L.), which have been reported as rare and vulnerable and have been identified as requiring the preparation and implementation of a conservation management plan. The presence of Arctic charr in Ennerdale Water has resulted in it being designated as a Site of Special Scientific Interest. These fish and one race of the species in Lake Windermere are the only English populations of charr known to spawn in running water. Associated with Ennerdale charr is the copepod parasite Salmincola edwardsii which has not been recorded from any other charr inhabited waters of the Lake District. However, it has been recorded on charr from four Scottish Lochs (Stack, Lee, Tay and Doon). The unique nature of Ennerdale is further highlighted by the presence of two crustaceans, Mysis relicta and Limnocalanus macrurus. The former has been recorded in Ireland while the latter is not known to exist anywhere else in the British Isles. The aim of this pilot study was to obtain baseline data on charr that spawn in Smithy Beck and the River Liza. This would indicate the current status of the population and help identify areas requiring further investigation. A total of 161 fish (95 males and 66 females) was caught and tagged over the 3 day period, 141 from Smithy Beck and 20 from the Liza. The raw data of the findings is presented in two appendices.

Systematics of the North American menhadens: molecular evolutionary reconstructions in the genus Brevoortia (Clupeiformes: Clupeidae)

Evolutionary associations among the four North American species of menhadens (Brevoortia spp.) have not been thoroughly investigated. In the present study, classifications separating the four species into small-scaled and large-scaled groups were evaluated by using DNA data, and genetic associations within these groups were explored. Specifically, data from the nuclear genome (microsatellites) and the mitochondrial genome (mtDNA sequences) were used to elicit patterns of recent and historical evolutionary associations. Nuclear DNA data indicated limited contemporary gene flow among the species, and also indicated higher relatedness within the small-scaled and large-scaled menhadens than between these groups. Mitochondrial DNA sequences of the large-scaled menhadens indicated the presence of two ancestral lineages, one of which contained members of both species. This result may indicate genetic diver-gence (reproductive isolation) followed by secondary contact (hybridization) between these species. In contrast, a single ancestral lineage indicated incomplete genetic divergence between the small-scaled menhaden. These results are discussed in the context of the biology and demographics of each species.

Life history characteristics for silvergray rockfish (Sebastes brevispinis) in British Columbia waters and the implications for stock assessment and management

We summarize the life history characteristics of silvergray rockfish (Sebastes brevispinis) based on commercial fishery data and biological samples from British Columbia waters. Silvergray rockfish occupy bottom depths of 100−300 m near the edge of the continental shelf. Within that range, they appear to make a seasonal movement from 100−200 m in late summer to 180−280 m in late winter. Maximum observed age in the data set was 81 and 82 years for females and males, respectively. Maximum length and round weight was 73 cm and 5032 g for females and 70 cm and 3430 g for males. The peak period of mating lasted from December to February and parturition was concentrated from May to July. Both sexes are 50% mature by 9 or 10 years and 90% are mature by age 16 for females and age 13 years for males. Fecundity was estimated from one sample of 132 females and ranged from 181,000 to 1,917,000 oocytes and there was no evidence of batch spawning. Infection by the copepod parasite Sarcotaces arcticus appears to be associated with lower fecundity. Sexual maturation appears to precede recruitment to the trawl fishery; thus spawning stock biomass per recruit analysis (SSB/R) indicates that a F50% harvest target would correspond to an F of 0.072, 20% greater than M (0.06). Fishery samples may bias estimates of age at maturity but a published meta-data analysis, in conjunction with fecundity data, independently supports an early age of maturity in relation to recruitment. Although delayed recruitment to the fishery may provide more resilience to exploitation, managers may wish to forego maximizing economic yield from this species. Silvergray rockfish are a relatively minor but unavoidable part of the multiple species trawl catch. Incorrectly “testing” the resilience of one species may cause it to be the weakest member of the specie

Genetic variation of rougheye rockfish (Sebastes aleutianus) and shortraker rockfish (S. borealis) inferred from allozymes

Rougheye rockfish (Sebastes aleutianus) and shortraker rockfish (Sebastes borealis) were collected from the Washington coast, the Gulf of Alaska, the southern Bering Sea, and the eastern Kamchatka coast of Russia (areas encompassing most of their geographic distribution) for population genetic analyses. Using starch gel electrophoresis, we analyzed 1027 rougheye rockfish and 615 shortraker rockfish for variation at 29 proteincoding loci. No genetic heterogeneity was found among shortraker rockfish throughout the sampled regions, although shortraker in the Aleutian Islands region, captured at deeper depths, were found to be significantly smaller in size than the shortraker caught in shallower waters from Southeast Alaska. Genetic analysis of the rougheye rockfish revealed two evolutionary lineages that exist in sympatry with little or no gene f low between them. When analyzed as two distinct species, neither lineage exhibited heterogeneity among regions. Sebastes aleutianus seems to inhabit waters throughout the Gulf of Alaska and more southern waters, whereas S. sp. cf. aleutianus inhabits waters throughout the Gulf of Alaska, Aleutian Islands, and Asia. The distribution of the two rougheye rockfish lineages may be related to depth where they are sympatric. The paler color morph, S. aleutianus, is found more abundantly in shallower waters and the darker color morph, Sebastes sp. cf. aleutianus, inhabits deeper waters. Sebastes sp. cf. aleutianus, also exhibited a significantly higher prevalence of two parasites, N. robusta and T. trituba, than did Sebastes aleutianus, in the 2001 samples, indicating a possible difference in habitat and (or) resource use between the two lineages.

Lethal Parasites in Oysters from Coastal Georgia with Discussion of Disease and Management Implications

Extensive mortalities of oysters, Crassostrea virginica, occurred from 1985 through 1987 in coastal waters of Georgia. Fluid thioglycolate cultures of oysters collected from 16 of 17 locations revealed infections by the apicomplexan parasite Perkinsus marinus. An ascetosporan parasite, Haplosporidium nelsoni, was also observed in histopathological examination of oysters from 4 of the locations. While the range of H. nelsoni currently is recognized as the east coast of the United States from Maine to Florida, this is the first report of the parasite in Georgia waters. This paper documents the occurrence of these two lethal parasites in oysters from coastal waters of Georgia, along with potential disease and management implications. Results of an earlier independent and previously unpublished survey are also discussed which document the presence of P. marinus in Georgia as early as 1966.

A comparative survey of the fish populations of 10 West-Midlands meres of SSSI status

This is a comparative survey of the Fish Populations of 10 West-Midlands Mere of Site of Special Scientific Interest Status. The meres of the Shropshire-Cheshire plain (West Midlands) comprise over 60 water bodies. Water quality in the meres ranges from naturally eutrophic to nutrient limited and some of them have received Site of Special Scientific Interest status (SSSIs). This survey was commissioned in order to obtain quantitative information on the fish fauna of selected SSSIs and evaluate the likely impact of fish on other species within the community. The current survey was concerned with 10 of the West Midlands’ meres, a cross section of sites was selected. Sites were widely distributed from Marton Pool in the south-western part of Shropshire to Tabley moat in Chesire. Meres varied in size, depth and nutrient status. All were to be sampled using the methods outlined. The report is divided into different sections for clarity and ease of reference. The materials and methods section provides an outline of the location and status of each mere and information on known fish species present. The results were considered individually and comparatively, relative abundance of fish, species present, their diet, sex, age, parasite burdens and growth. Inter-site comparisons were made using information derived from echo-soundings, growth rate, diet, and species abundance for each site. The final section will be in the form of a general discussion, comparative information from longer term studies, conclusions and recommendations.

A new species of western Atlantic lizardfish (Teleostei: Synodontidae: Synodus) and resurrection of Synodus bondi Fowler, 1939, as a valid species from the Caribbean with redescriptions of S. bondi, S. foetens (Linnaeus, 1766), and S. intermedius (Agassiz, 1829)

Western Atlantic synodontid species were studied as part of an ongoing effort to reanalyze Caribbean shorefish diversity. A neighbor-joining tree constructed from cytochrome c oxidase I (COI) data revealed 2 highly divergent genetic lineages within both Synodus intermedius (Agassiz, 1829) (Sand Diver) and S. foetens (Linnaeus, 1766) (Inshore Lizardfish). A new species, Synodus macrostigmus, is described for one of the S. intermedius lineages. Synodus macrostigmus and S. intermedius differ in number of lateral-line scales, caudal pigmentation, size of the scapular blotch, and shape of the anterior-nostril flap. Synodus macrostigmus and S. intermedius have overlapping geographic and depth distributions, but S. macrostigmus generally inhabits deeper water (>28 m) than does S. intermedius and is known only from coastal waters of the southeastern United States and the Gulf of Mexico, in contrast to those areas and the Caribbean for S. intermedius. Synodus bondi Fowler, 1939, is resurrected from the synonymy of S. foetens for one of the S. foetens genetic lineages. The 2 species differ in length and shape of the snout, number of anal-fin rays, and shape of the anterior-nostril flap. Synodus bondi and S. foetens co-occur in the central Caribbean, but S. bondi otherwise has a more southerly distribution than does S. foetens. Redescriptions are provided for S. intermedius, S. foetens, and S. bondi. Neotypes are designated for S. intermedius and S. foetens. A revised key to Synodus species in the western Atlantic is presented.

Assessment of contaminant body burdens and histopathology of fish and shellfish species frequently used for subsistence food by Alaskan Native communities

Subsistence food items can be a health concern in rural Alaska because community members often rely on fish and wildlife resources not routinely monitored for persistent bioaccumulative contaminants and pathogens. Subsistence activities are a large part of the traditional culture, as well as a means of providing protein in the diets for Tribal members. In response to the growing concerns among Native communities, contaminant body burden and histopathological condition of chum and sockeye salmon (Oncorhynchus keta and Oncorhynchus nerka) and the shellfish cockles and softshell clams (Clinocardium nuttallii and Mya arenaria) were assessed. In the Spring of 2010, the fish and shellfish were collected from traditional subsistence harvest areas in the vicinity of Nanwalek, Port Graham, and Seldovia, AK, and were analyzed for trace metals and residues of organic contaminants routinely monitored by the NOAA National Status & Trends Program (NS&T). Additionally, the fish and shellfish were histologically characterized for the presence, prevalence and severity of tissue pathology, disease, and parasite infection. The fish and shellfish sampled showed low tissue contamination, and pathologic effects of the parasites and diseases were absent or minimal. Taken together, the results showed that the fish and shellfish were healthy and pose no safety concern for consumption. This study provides reliable chemistry and histopathology information for local resource managers and Alaska Native people regarding subsistence fish and shellfish use and management needs.

The use of parasites in discriminating stocks of Pacific halibut (Hippoglossus stenolepis) in the northeast Pacific

The use of parasites as indicators of the stock structure of Pacific halibut (Hippoglossus stenolepis) in the northeast Pacific was investigated by using 328 adult (>55 cm fork length) halibut from 15 composite localities ranging from northern California to the northern Bering Sea and 96 juvenile (10–55 cm) halibut from five localities ranging from the northern Queen Charlotte Islands to the Bering Sea. Counts of eight selected parasite species (the juvenile acanthocephalans Corynosoma strumosum and C. villosum, the metacestode Nybelinia surmenicola, the digenean metacercaria Otodistomum sp., and the larval nematodes Anisakis simplex, Pseudoterranova decipiens, Contracaecum sp., and Spirurid gen. sp.) that produce infections of long duration, do not multiply in the host, and that have a relatively high abundance in at least one geographic locality were subjected to discriminant function analysis. Juvenile Pacific halibut showed no separation and, even though they were not heavily infected with parasites, the analysis suggested that juveniles could be a mixed stock. Three groups of adults were identified: fish from California to the southern Queen Charlotte Islands, those from the northern Queen Charlotte Islands to the central Bering Sea, and those from the central and north-ern Bering Sea. These groups suggest that the single stock concept be more thoroughly evaluated.

Gill pathology of juvenile carps in nursery ponds

Gill pathology of juveniles of two Indian major carps Labeo rohita and Cirrhinus mrigala were studied for a culture period of three months in a private and a government fish farm pond. Under histopathological observations, only protozoan parasite, Myxobolus sp., was recorded as cyst. These myxosporidian cysts were high in the gills of L. rohita of the government farm pond followed by C. mrigala of the private farm pond. Hypertrophic gill lamellae (primary and secondary) with loss of secondary lamellae were evidenced in C. mrigala of privately operated pond.

A Review of the parasitic copepods of fish recorded from Ceylon with description of additional forms

The beginning of our knowledge of the copepods parasitic on fish from Ceylon is due to Bassett-Smith (1898 a) who, in a paper on "Further New Parasitic Copepods found on Fish in the Indo-Tropical Region", included seven species collected at Trincomalee and Colombo. Later in the same year, in a paper on "Some New or Rare Parasitic Copepods from the Indo-Tropical Region", he (Bassett-Smith, 1898 b) included three more species from Ceylon. Soon after, more of these parasites were obtained from Ceylon during Herdmann's investigation of the Pearl Banks. From this collection, one lot consisting of eleven species was described by Thompson and Scott (1903) and a second lot consisting of seven species was described by Wilson (1906). At that stage the number of species recorded from Ceylon made up to a total of twenty-eight and there the matter rested for another quarter of a century until, quite by chance, while collecting marine animals on a reef, Mr Kirtisinghe came across a newly dead half-beak with a learned parasite projecting from its body. Since then, in a number of occasional papers (Kirtisinghe, 1932-35, 1937, 1950, 1956, 1960) he has described thirty-eight more species of parasitic copepods from Ceylon. However, his collection included many more species which were put aside for later attention. In the present paper, while dealing with those forms in his collection which he has not recorded or described earlier, he has put together all the known forms of parasitic copepods of fish from Ceylon. A list of the host fishes with their respective parasitic copepods is also provided, types of new species, at present in the author's private collection, will be deposited in the Fisheries Department, Colombo, Ceylon.

A Preliminary survey of 21 Ceylon lakes. 3. Parasites and predators, food of fish, and marginal fauna

Three nematodes, two acanthocephalans and three cestodes were collected by dissecting 15 species of fish. Of these parasites only Hedruris sp. a larval nematode is responsible directly for the death of fish, since it is found in predatory species a reduction of these might prove of considerable consequence to fish, production. Bothriocephalus gowleonensis, a cestode probably introduced from China with Chinese carp, was recorded in an indigenous carp Puntius sarana. This parasite causes heavy mortality in grass carp in China and is a potential danger to local species. A single crustacean parasite was recorded. It is likely that they are widespread. No protozoa were recorded but there can be little doubt that they occur in local freshwater species and may be of considerable importance in fisheries. Several predators (insects, fish and other vertebrates) were recorded and are doubtless of considerable importance. However, the role of parasites and of predators (especially fishes) in relation to the productivity of fisheries can hardly be assessed at present and needs further study. A study of the fauna found at the shallow edges of 21 irrigation reservoirs showed an abundance of fauna in the low-country reservoirs whilst the up-country reservoirs had a poor fauna. The fauna showed insects, crustacea and mollusca in this order of abundance. The presence of insect and molluscs feeding fish in our fauna shows that these rich sources of food are being utilised.

Some parasitic Copepoda from Sri Lanka (Ceylon) with a synopsis of parasitic Crustacea from Ceylonese freshwater fishes

This report comprises a summary of parasitic copepods from fishes in Ceylon, as isolated from the branchial material of fishes belonging to previous collections. Seven copepod species are described in detail, as well as one species of Branchiura and one species of Isopoda. Caution is advocated to avoid further introductions via parasite-infested fishes, since only four of the above species are endemic.

Results of the Austrian-Ceylonese hydrobiological mission 1970 of the 1st Zoological Institute of the University of Vienna (Austria) and the Department of Zoology of the Vidyalankara University of Ceylon, Kelaniya. Pt. 7. Dryopidae and Elminthidae of Ceylon

This report comprises a summary of parasitic copepods from fishes in Ceylon, as isolated from the branchial material of fishes belonging to previous collections. Seven copepod species are described in detail, as well as one species of Branchiura and one species of Isopoda. Caution is advocated to avoid further introductions via parasite-infested fishes, since only four of the above species are endemic.

Distribution, prevalence and intensity of protozoan and monogenean parasites of carp fingerlings in selected nursery ponds

An experiment was carried out on the distribution, prevalence and intensity of infestation of protozoan and monogenean parasites of carp fingerlings in two selected areas of Bangladesh. Six hundred and forty fingerlings of seven species viz. Catla calla, Hypophthalmicthys molitrix, Labeo rohita, Ctenophalyngodon idella, Cyprinus carpio, Barbodes gonionotus and Cirrhinus cirrhosus from different nursery ponds of Shambhuganj, Mymensingh, and Santaher, Bogra were examined during this investigation. This study revealed that carp fingerlings carried a large number of protozoan and monogenean parasites. Different protozoan parasites viz Trichodina domerguei, Trichodina reticulata, Myxobolus koi, Chilodonella cyprini and monogenean parasites such as Dactylogyrus extensus, Dactylogyrus catlarius and Dactylogyroides tripathi were identified in the experimental fishes throughout the study period. It has been observed that distribution, prevalence and intensity of parasite in carp fingerlings are species and zone specific. In case of C. cirrhosus, the highest prevalence of protozoan parasite viz Trichodina domerguei were 93.75% and 75.92% in Santhahar and Shambhuganj area respectively. Irrespective of host, the most prevalent ectoparasite was Dactylogyrus extensus, followed by Myxobolus koi, Chilodonella cyprini and Dactylogyroides tripathi. Relatively higher mortality of carp fingerlings was found at younger stage. The highest prevalent parasitic species was identified as Trichodina domerguei followed by Trichodina reticulata and Dactylogyrus catlarius were found 93.75% in Cirrhinus cirrhosus, 68.75% in Cyprinus carpio and 39.44% in Hypophthalmicthys molitrix respectively.