Monoculture of silver barb (Puntius gonionotus Bleeker) in rice floodwaters in Bangladesh

On-station and on-farm trials with silver barb (Puntius gonionotus) monoculture in rice floodwaters in Bangladesh are summarized and discussed in this article.

How can research best serve the needs of aquaculture in subsaharan Africa?

Discussion on whether research is the answer to best serve the needs of aquaculture in SubSaharan Africa is presented. New methods of user-participatory, on-farm research being tested by ICLARM and other agencies in Asia and Africa is discussed.

Making experiment station results more useful to African fish farmers

Rather than using more or less ideal conditions for setting experimental controls, the use of conditions similar to those likely to be encountered by farmers should produce research results which are realistically achievable on the farm. ICLARM has developed an approach to farmer-led experimentation which utilizes a spreadsheet to collate and analyze data collected from participating farmers. The simulation of actual management practices utilized by farmers produced results in replicated on-station trials which were within 11% of net yields on-farm. In addition to giving researchers a tool for comparing farm and station management practices, giving farmers a realistic indication of what yields will be if a technology is adopted should help overcome the problems of disillusionment often encountered when farm results fall below those expected by researchers on the basis of experiment station studies.

Integrating fish and azolla into rice-duck farming in Asia

Several countries in Asia practice integrated rice-duck farming. On-farm resources such as duck manure and feed waste are not adequately used and recycled in the system. This indicates the potential for research to increase the productivity of the rice-duck system. The integration of fish and the nitrogen-fixing aquatic fern azolla show promise for increasing the production potential of the system. Fish, azolla and ducks integrated with rice farming can result in nutrient enhancement, pest control, feed supplementation and biological control. Some of the results of a case study on integrated rice-fish-azolla-duck farming systems conducted in the Philippines are presented in this paper.

Impact of development and dissemination of Integrated Aquaculture-Agriculture (IAA) Technologies in Malawi

Malawi is a small but densely populated country in Southern Africa. Fish is an important part of the nutrition of Malawians, providing essential protein and micronutrients. However, per capita fish consumption has halved over the ten-year period between 1988 to 1998 due to over-fishing in the lakes and doubling of the population since the 1970s, accompanied by an increase in the price of fish. This has worsened access to food insecurity, especially in rural areas, in a country where an estimated 66 per cent of the population consume less than the minimum daily calorie requirement. This paper presents an ex-post impact assessment of the development and dissemination of smallscale integrated aquaculture-agriculture technologies by The WorldFish Center and its national and international partners over more than 15 years in Malawi. The impact study measures the effects of these outputs on the degree of integrated aquaculture-agriculture (IAA) technology adoption and diffusion, the effects on farm income and health of household members, and the welfare effects of increased fish supply on the Malawian economy.

Markets, marketing and production issues for aquaculture in East Africa: the case of Uganda

Aquaculture is currently responsible for an insignificant proportion of total fish production in Uganda. However, given the increasing demand for fresh fish in urban and peri-urban araes, and threats to the supply of fish from natural catch fisheries, the potential exists for a strong market in aquaculture. Small-scale fish farmers located relatively close to markets or all-season roads, and who can supply consistent and high quality produce, will have the widest range of marketing opportunities, and will likely be within the area of operation of potential traders and intermediaries that deliver fish to markets. Fish farmers that are not close to roads, or produce unreliable quantities and variable quality products may face high transaction costs of marketing their product, and decreasing net returns to production. The authors found that significant on-farm labor, and access to input markets are important factors leading to positive net returns to fish production. Areas with high population density and relatively low wages will be well suited to labor intensive aquaculture. The authors concluded that aquaculture development has good potential in certain areas of Uganda and should therefore be pursued as a potential development pathway. However, policy makers should consider the importance of the price of fresh fish relative to the cost of labor, as well as other factors including the importance of smallholder credit and access to extension services, when directing investments in aquaculture technology.

Farmer-scientist research partnerships for integrated agriculture-aquaculture

A realistic alternative to traditional technology development and transfer has been utilized by the International Center for Living Aquatic Resources Management (ICLARM) to integrate pond fish culture into low-input farming systems in Malawi. Resource mapping was used to assess farm resources and constraints and introduce the concept of integrated resource management (IRM), the synergistic movement of resources between and among farm and household enterprises. Farmer-led IRM research projects are conducted on-farm and monitored by researchers through direct observation and on-station simulation of constraints and management practices. Technology-adoption rates by farmers involved in a pilot activity was 65% compared to 0% by farmers exposed only to top-down extension approaches. Within two years of adoption, every participating farmer had transferred the technology to an average of four other farmers without the involvement of the extension services.

Integrated aquaculture-agriculture adoption and impact in rainfed farming systems in Sakata, Malawi

ICLARM introduced integrated aquaculture-agriculture (IAA) in Sakata, Malawi three years ago. Since that time, and without extension support, the number of farmers with ponds increased from 4 in 1993/94 to 12 in 1995/96. To learn why and how IAA is spreading, a study of impact and adoption was conducted in the 1995/96 production season. Interviews were conducted with farmers to discuss lAA and collect data on farm function through the use of bioresource flow diagrams. Motivations given by farmers as to why they adopted IAA were to improve household nutrition and income. Constraints to adoption identified by farmers were availability of labor and capital to purchase inputs

Performance of genetically improved farmed tilapia (GIFT) under mono and mixed cui ture with silver barb (Barbodes gonionotus) in south-west Bangladesh

A participatory on-farm trial was carried out to evaluate the production performance of GIFT (genetically improved farmed tilapia) strain of Oreochmis sp., either alone or with silver barb (Barbodes gonionotus), in six rain-fed freshwater ponds of coastal area. There vvere two treatments; (i) GIFT alone at a stocking density of 24,700/ha (T1) and (ii) 1:1 combination of GIFT and silver barb (T1). Each of the treatments had three replications. A significantly (p

Polyculture of carps using over-wintered fingerlings under different stocking densities

An on-farm trial was undertaken in twelve earthen ponds (1200-1600 m') to evaluate the growth and production of over-wintered fingerlings of rohu (Labeo rohita), catla (Carla catla) and mrigal (Cirrhinus rnrigala) in polyculture at three stocking densities. The stocking densities were 2,250; 3,250 and 4,250 fish/ha in treatment-1, treatment-2 and treatment-3, respectively. Fish in all ponds were fed with rice bran and mustard oil cake at the ratio of 3:1. Fish production obtained in three treatments were 2325±74.75, 2620±49.66 and 2982±171.52 kg/ha. The results demonstrated higher mean growth in T-1 than in T-2 and T-3. However, the highest production as well as net benefit was obtained in treatment-3.

Culture potentials of bata, Labeo bata under semi-intensive management system

Small indigenous fish species (SIS) provide food, nutrition, subsistence and supplemental income to a great majority of the people particularly the poor and disadvantaged people of Bangladesh. To date nobody explored the possibilities of culturing these species in combination with the indigenous major carps viz. catla, Catla catla; rohu, Labeo rohita, and mrigal Cirrhinus cirrhosus and grass carp, Ctenopharyngodon idellus. An experiment on the polyculture of carps with a popular SIS, bata (Labeo bata) was carried out to evaluate the production performance of carp-SIS culture in on-farm condition during 15 March to 15 September 2003. Three treatments each having three replications with different stocking densities of bata were tested keeping the carp species combination and stocking density similar in all the treatments viz. treatment 1 (T1), bata (5,000/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha); treatment 2 (T2), bata (7,500/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha); and treatment 3 (T3), bata (1 0,000/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha). After six months rearing, the production obtained were 2,466±77, 2,395±85 and 2,074±72 kg/ha from T1, T2 and T3, respectively. The highest production was obtained from T1, where the stocking density of bata was the minimum (5,000/ha) while the lowest production was obtained from T1 [sic], where the stocking density of bata was maximum (10,000/ha). Significant difference (P<0.05) exists in the production levels as obtained from different treatments. The contribution of bata to total production was 10.31%, 13.96% and 14.38% in case of T1, T2 and T3, respectively.

Rapid detection of white spot syndrome virus (WSSV) of Penaeus monodon by latex agglutination test using monoclonal antibodies

Latex beads were sensitized with monoclonal antibodies (MAb) rose against VP28 of WSSV. The optimum concentration of MAb required to sensitize the latex beads was 125 µg/ml. The sensitized latex beads were used to detect WSSV from PCR-positive stomach tissue homogenates obtained from infected shrimp. Stomach tissue homogenates from WSSV-infected shrimp agglutinated the sensitized latex beads within 10 minutes, while uninfected samples did not produce any agglutination, although non-specific agglutinations were observed in some samples. The analytical sensitivity, analytical specificity, diagnostic sensitivity and diagnostic specificity of the (LAT) agglutination test were assessed. The analytical sensitivity of the test was 40 ng of purified WSSV (2 µg/ml). The sensitized latex beads did not agglutinate with normal shrimp tissue or MBV-infected tissue homogenate. The test has a diagnostic sensitivity of 70 and 45%, respectively, compared to single-step and nested PCR. The diagnostic specificity of the test was 82%. This test is a simple and rapid on-farm test which can be used to corroborate clinical signs for the detection of WSSV in grow-out ponds.

Seed production and culture techniques of Genetically Improved Farmed Tilapia (GIFT) in brackishwater environment

Effects of different levels of salinity on survival, growth and gonadal development of Genetically Improved Farmed Tilapia (GIFT) were studied under laboratory conditions in glass aquarium, for a period of ten weeks. The initial individual size of the GIFT was 20.23±4.45 and the salinity levels tested were 0, 5, 10, 15 and 20 ppt. The highest survival of 87.5% was found in 0 ppt and the lowest 60.5% in 20 ppt. Though the survival decreased progressively with increased salinity, there were no significant differences (P>0.05) among 0, 5, and 10 ppt. Similar to what has been observed in survival, the specific growth rate (SGR %/day) also decreased as of 1.30, 1.24, 1.08, 0.90 and 0.71, respectively, with the increased salinity of 0, 5, 10, 15 and 20 ppt. The gonadal development was highest in 0 ppt with a GSI value of 3.75 and lowest of 2.01 in 20 ppt. In the second experiment, gonadal development and seed production performance of GIFT in brackishwater condition were investigated for a period of three months. Each of the three fine meshed hapas of 20 square meters made from nylon net was placed in a freshwater (0 ppt) and in a brackish water (10-15 ppt) pond of the Brackishwater Station (BS). GIFT of 65 g average weight from a single cohort were stocked into three hapas at a rate of 2 per m. The male vs female ratio was 1:3. The development of gonad was faster with the higher gonadosomatic index (GSI %) of 3.85 % in freshwater condition than that of 2.73 % in brackish water. Within three months of the study period, a total of 70,510 and 44,250 GIFT fry were produced respectively, in freshwater and brackishwater conditions. Finally under third experiment, a participatory on-farm trial was carried out to evaluate the production performance of GIFT in monoculture and in polyculture with silver barb in coastal freshwater pond conditions. Nine ponds were selected for three treatment combinations of GIFT monoculture (T1), GIFT and silver barb polyculture (T2), and silver barb monoculture (T3). The ponds have been stocked in April, 05 at a density of 25,000 fry per ha. Fishes were fed with rice bran at the rate of 6% bw per day. In one month culture period, GIFT attained an average weight of 16.27 g in monoculture and 17.23 g in polyculture, against an average stocking weight of 0.37 g. Silver barb reached an average weight of 16.62 g in polyculture with GIFT and 10.01 g in monoculture, against an average stocking weight of 3.79 g.

Monoculture of three native air breathing fishes in homestead ditches and plastic barrels

Six on-farm trials were conducted from 1 August to 23 November 2004 in two different environments such as homestead ditches (10 to 17 square meters) and plastic barrels (240 liters) to develop techniques for nonoculture [sic] of climbing perch, Anabas testudineus, stinging catfish, Heteropneustes fossilis and walking catfish, Clarias batrachus for poor and landless people who have no access to pond. Stocking density for ditch was 10 fry/square meter while that for barrel was 20 fry/cubic meter. The fishes were fed with 3-test diets viz. low-cost formulated feed (rice bran 20%, wheat meal 10%, mustered [sic] oil cake 35%, poultry offal 35%), live foods (chopped snails and clams), and a commercial feed (Saudi-Bangla feed, starter 3: first month and grower-1: subsequent two months) and designated as T1, T2 and T3, respectively. Feeding rate was the same in all the treatments viz. 10% of body weight (first two months), 8% (third month) and 6% (fourth month). T1 and T2 had three replications while T3 had two replications. Water temperature was recorded weekly while fish growth was monitored monthly. After 4 months' rearing, H. fossilis and C. batrachus in ditches and barrels attained higher average weight in T2 followed by T3 and T1 while A. testudineus in barrels also attained higher average weight in T2. The variation in net weight gain by A. testudineus in ditch fed test diets T2 and T3 was not significantly different (P>0.05) though the net gain in both T2 and T3 was significantly (P<0.05) higher than that of T1. The yield of climbing perch as obtained from T1, T2 and T3 was 988, 1136 and 1185 kg/ha, respectively while that stinging catfish was 395, 242 and 444 kg/ha and walking catfish was 1605, 2,099 and 1,654 kg/ha respectively. All the three species showed significantly lower growth rate in barrels than in ditches.

Nursery management system of stinging catfish, Heteropneustes fossilis and walking catfish, Clarias batrachus in Bangladesh

A total of four experiments were conducted to develop nursery management system for the two important native catfishes viz. stinging catfish, Heteropneustes fossilis and walking catfish, Clarias batrachus during 2003 and 2004. Two experiments were conducted in on-station condition to determine stocking density efficacy in hapa and in earthen mini ponds for H. fossilis. This was followed by on-farm trial on stocking density in earthen mini ponds. In hapa, the highest survival rate was 60% for H. fosssilis at stocking density of 100/square meter; followed by 54.5 and 50% at stocking densities of 150 and 200/square meter, respectively. Similarly, gain in weight and length was also better in lower stocking densities. Similar survival rate (45 to 55%) was observed in three densities in earthen mini ponds. However, gain in weight and length showed no significant (p<0.05) differences. H. fossilis, C. batrachus showed almost similar survival rates (57.5-59.0%) in all the three stocking densities (100, 150 and 200/square meter) in earthen mini ponds in on-station condition. In terms of gain in weight and length showed no significant (p<0.05) differences in pond conditions. Almost similar result was obtained in terms of growth and survival in on-farm condition.