A Preliminary survey of 21 Ceylon lakes. 3. Parasites and predators, food of fish, and marginal fauna

Three nematodes, two acanthocephalans and three cestodes were collected by dissecting 15 species of fish. Of these parasites only Hedruris sp. a larval nematode is responsible directly for the death of fish, since it is found in predatory species a reduction of these might prove of considerable consequence to fish, production. Bothriocephalus gowleonensis, a cestode probably introduced from China with Chinese carp, was recorded in an indigenous carp Puntius sarana. This parasite causes heavy mortality in grass carp in China and is a potential danger to local species. A single crustacean parasite was recorded. It is likely that they are widespread. No protozoa were recorded but there can be little doubt that they occur in local freshwater species and may be of considerable importance in fisheries. Several predators (insects, fish and other vertebrates) were recorded and are doubtless of considerable importance. However, the role of parasites and of predators (especially fishes) in relation to the productivity of fisheries can hardly be assessed at present and needs further study. A study of the fauna found at the shallow edges of 21 irrigation reservoirs showed an abundance of fauna in the low-country reservoirs whilst the up-country reservoirs had a poor fauna. The fauna showed insects, crustacea and mollusca in this order of abundance. The presence of insect and molluscs feeding fish in our fauna shows that these rich sources of food are being utilised.

Induced spawning of two major species of Chinese carps, Ctenopharyngodon idellus and Aristychthys nobilis in Sri Lanka

Induced spawning of grass carp (Ctenopharyngodon idellus) and bighead carp (Aristychthys nobilis) was carried out successfully in Sri Lanka for the first time in 1977 and 1978, respectively. This article describes the techniques involved in induced spawning in Sri Lanka at the Freshwater Fish Breeding and Experimental Station in Udawalawa.

Polyculture of freshwater prawn, Macrobrachium rosenbergii, de Man with carps: effects of prawn stocking density

Effects of three stocking densities, viz., 35, 50 and 65/decimal (1 decimal = 40.48 m2) of juvenile freshwater prawn (Macrobrachium rosenbergii) on prawn and fish production were tested in a polycuture system with silver carp (Hypophthalmichthys molitrix), catla (Catla catla), Grass carp (Ctenophmyngodon idella) and silver barb (Barbodes gonionotus). The fish stocking density was 19/decimal with the species combination of silver carp-10, catlac-6, grass carp-1 and silver barb-2. In a 8-month culture period, the prawn yield 423 ± 144 kg/ha was significantly lower (P< 0.5) with the prawn stocking density of 35/decimal than that of 548 ± 178 kg/ha and 662 ± 243 kg/ha with 50 and 65/decimal respectively. The fish production (1844-1891 kg/ha) did not differ significantly (p <0.05) among the three treatments indicating that prawn stocking densities had no influence on fish yield. The lower mean harvest weight (62 g) and survival rate (67 g) and higher yield (2.67 kg/decimal) with the highest stocking rate of prawn reveals that as density was increased, prawn survival and individual weight at harvest decreased but total yield increased.

Culture potentials of bata, Labeo bata under semi-intensive management system

Small indigenous fish species (SIS) provide food, nutrition, subsistence and supplemental income to a great majority of the people particularly the poor and disadvantaged people of Bangladesh. To date nobody explored the possibilities of culturing these species in combination with the indigenous major carps viz. catla, Catla catla; rohu, Labeo rohita, and mrigal Cirrhinus cirrhosus and grass carp, Ctenopharyngodon idellus. An experiment on the polyculture of carps with a popular SIS, bata (Labeo bata) was carried out to evaluate the production performance of carp-SIS culture in on-farm condition during 15 March to 15 September 2003. Three treatments each having three replications with different stocking densities of bata were tested keeping the carp species combination and stocking density similar in all the treatments viz. treatment 1 (T1), bata (5,000/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha); treatment 2 (T2), bata (7,500/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha); and treatment 3 (T3), bata (1 0,000/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha). After six months rearing, the production obtained were 2,466±77, 2,395±85 and 2,074±72 kg/ha from T1, T2 and T3, respectively. The highest production was obtained from T1, where the stocking density of bata was the minimum (5,000/ha) while the lowest production was obtained from T1 [sic], where the stocking density of bata was maximum (10,000/ha). Significant difference (P<0.05) exists in the production levels as obtained from different treatments. The contribution of bata to total production was 10.31%, 13.96% and 14.38% in case of T1, T2 and T3, respectively.

On farm trial of Bangladesh Fisheries Research Institute (BFRI) evolved two aquaculture technologies

Two BFRI evolved aquaculture technologies - integrated rice fish farming and carp polyculture with over-wintered fingerlings under different stocking densities were tested during 2003-04. The study was coordinated with two local NGOs namely NICHAITA and JNDP, Muktagacha, Mymensingh. Integrated rice fish farming technology was demonstrated in 9 plots each having an area between 60-100 dec. during boro season. Fifteen days after transplantation of rice seedlings, fingerlings of rajpunti (Barbodes gonionotus) of 7-10 g of individual weight were stocked in the rice fields at the density of 3,000 (T1), 3,750 (T2) and 4,500/ha (T3). The corresponding final weight of fish after three and half months in treatments 1, 2 and 3 were 110±14.21, 101±16.55 and 86±22.28 g, respectively. The mean weight of fish in treatments 1 and 2 was significantly higher than treatment 3. Fish production obtained from treatments 1, 2 and 3 were 218.16±18.29, 239.70±25.11 and 236±24.66 kg/ha, respectively. On-farm demonstrations of carp polyculture using over-wintered fingerlings rohu (25-28 g), catla (24-26 g), mrigal (21-26 g) and grass carp (20-24 g) under different stocking densities were undertaken in nine earthen ponds (1,200-1,600 square meters) for a period of six months at three different stocking densities. The stocking densities of treatment 1 (T1), treatment 2 (T2) and treatment 3 (T3) were 2,000, 3,000 and 4,000/ha, respectively. Fish were fed with rice bran and mustard oil cake (3:1). Soft green grass and banana leaves were provided mainly for grass carp. At harvest, the production obtained in treatments 1, 2 and 3 were 2,325±74.75, 2,620±49.66 and 2,982± 171.52 kg/ha, respectively. The results demonstrated higher growth of fish in treatment 1 than those of treatments 2 and 3. However, treatment 3 contributed relatively higher production than those of treatments 1 and 2, whereas, highest net benefit was received from treatment 2.

Shifting rice farming to fish culture in some selected areas of Mymensingh, Bangladesh: the process, conflicts and impacts

The evolutionary process of converting low-lying paddy fields into fish farms and its impact on agrarian communities in some selected areas of Mymensingh district were studied. This study was conducted through participatory rural appraisal (PRA) covering 12 villages from each of selected upazillas viz. Fulpur and Haluaghat of Mymensing [sic] district. A total of 12 PRA sessions were conducted where 90 farmers participated during 29 July to 26 August 2004. It is seen that the use of low-lying paddy fields was mostly confined to Broadcast Aman (B. Aman) rice production until 1960s. With the introduction of modern rice farming technology, the farmers started to produce Boro rice in Rabi season and B. Aman rice in Kharif season. With the passage of time, aquaculture technologies have been evolved and the farmers realized that fish farming is more profitable than rice cultivation, and then they started to utilize their paddy fields for alternate rice-fish farming and rice-cum-fish farming. Now a days, aquaculture based crop production system is in practice in more than 25% of the low-lying paddy fields. Conversion of rice fields in to fish ponds has brought up a change in the livelihood patterns of the rural farmers. The areas where the farmers involved themselves in the new production systems were fingerling collection, transportation and marketing of fry and fingerlings. During 1960s to 1970s, a few people used to culture fish in the permanent ponds for their own consumption, the species produced were rohu, catla, mrigal, ghainna, long whiskered catfish, freshwater shark (boal), snake head (shol) etc. Small fishes like climbing perch, stinging catfish, walking catfish, barb, minnows etc. were available in the rice fields during monsoon season. In 1980s to mid 1990s, some rice fields were converted into fish ponds and the people started to produce fish for commercial purposes. When rice-fish farming became profitable, a large number of people started converting their rice fields in to rice-fish culture ponds. Culture of some exotic fishes like silver carp, tilapia, grass carp, silver barb etc. also started in the paddy fields. Higher income from fish farming contributed positively in improving the housing, sanitation and education system in the study areas. It is seen that the medium and medium high lands were only used for alternate rice fish farming. The net income was high in any fish based cropping system that motivated the farmers to introduce fish based cropping system in the low-lying inland areas. As a result, the regional as well as communal income disparities occurred. However, the extraction of ground water became common during the dry period as the water was used for both rice and fish farming. Mass conversion of paddy fields into rice-fish culture ponds caused water logging in the study areas. In most cases, the participated farmers mentioned that they could be easily benefited by producing fish with T. Aman or only fish during the monsoon season. They agreed that this was an impressive technology to them and they could generate employment opportunities throughout the year. Finally, the social, economic and technical problems which are acting as constraints to rapid expansion of fish production system were reported from the interviewee.

Demonstration of some selected aquaculture technologies under farming system research in Jessore and Santahar, Bangladesh

BFRI evolved some selected aquaculture technologies viz. polyculture of carps in perennial ponds, monoculture of short cycled fish species (BFRI super strain) in seasonal ponds and prawn seed production through backyard hatchery system have been demonstrated under Farming System Research (FSR) component in Jessore and Santahar regions. Both polyculture of carps and monoculture of short cycled fish species technologies were tested in farmer's ponds in Kaium Kula village near Jessore town. In polyculture trials, seven species comprising of silver carp (Hypophthalmichthys molirrix), catla (Catla catla), rohu (Labeo rohita), grass carp (Ctenopharyngodon idellus), common carp (Cyprinus carpio), mrigal (Cirrhinus cirrhosus) and silver barb (Barbonymus gonionotus) were stocked @ 9,500 (ratio 6:2:4:2:1:5:5); 10,750 (ratio 6:2:4:2:1:5:5) and 12,000 (ratio 6:2:4:2:1:5:4) fish/ha respectively in ponds of T1, T2 and T3 having three replications of each. The mean highest fish production was 3,148 kg/ha in T3, followed by 2,899 kg/ha in T1 and 2,875 kg/ha in T2. Production of T3 was significantly different (P<0.05) than both T1 and T2, while there was no significant differences (P>0.05) between the production of T1 and T2. In case of trial of short cycled fish species, two treatments were tested: T1 (comprising of BFRI super strain of Nile tilapia, silver carp, common carp and silver barb; ratio 3:5:1:1) and T2 (having only BFRI super strain of Nile tilapia). Stocking density in both the treatments were same (20,000 fish/ha). In this trial average production was higher in T1 (2,743 kg/ha) than that of T2 (2,369 kg/ha) but the production figure in these two treatments was not significantly different (P>0.05). Demonstration of backyard prawn hatchery technology was tested at Santahar region of Bogra district, North-west part of Bangladesh. This hatchery consisted of three main components i) bio-filter, ii) rearing tank unit (chari) and iii) air blower/air pump unit. Plastic drum of 200-250 l capacity and cemented chari of 200-250 l capacity were used as bio-filter and larval rearing containers respectively. A 0.5 hp air blower with 6 aquarium air pump were used to operate the aeration system in the hatchery. Diluted sea water (10-12 ppt) made from brine solution (200-250 ppt) collected from salt-bed was used in the backyard hatchery system of hatching of eggs and rearing of larvae. Rearing of first stage zoea-larvae was reared in three rearing tanks following the stocking densities of 40, 50 and 60/l of water respectively. Production of post-larvae were 20±0.82, 22±1.12 and 28±1.63/liter of water in treatments I, II and III respectively in 38, 40 and 39 days rearing period.

Culture potential of Amblypharyngodon mola with carps in polyculture in farmers' pond of northern regions of Bangladesh

To assess the culture potential of mola (Amblypharyngodon mola) along with carps in polyculture systems, an experiment consisted of three treatments each with five replications was conducted for 4 months in two villages of Parbatipur upazilla under Dinajpur district. In the first treatment (SS), catla, rohu, mrigal, grass carp, Thai punti, common carp and a higher density of silver carp (8 per 40m²) were stocked. In the second treatment (SM), stocking density of silver carp was reduced to half and mola was added at a stocking density of 12,500/ha with all other fishes used in SS. In the third treatment (MM), no silver carp was stocked and mola was added at a stocking density of 25,000/ha with all other fishes used in SS. All treatments were subjected to the same regime of feed and fertilizers. The yields of large carps were 2035 kg/ha, 1757 kg/ha and 1326 kg/ha for treatments SS, SM and MM, respectively. Catla, grass carp and carpio showed better growth and production performance in presence of mola at a higher density, while rohu, Thai punti and mrigal showed better performance when stocking density of mola was relatively low. Mola yield was almost two times higher (184 kg/ha) in absence of silver carp (MM) than (62 kg/ha) in presence of silver carp (SM). The result showed that there was a significantly (p<0.01) lower total fish production in treatment MM. But there were no significant difference in total production between treatment SS and SM.

Enhancement of fish production in a reservoir after partitioning by dikes through community participation

A reservoir of 70 acres was portioned by dikes into four manageable big ponds to get more production of fishes at Basurhat, Noakhali, Bangladesh under the supervision of local community through a society of 40 people ownership. Pangus (Pangasius hypophthalmus) @ 20,000/acre, and then fry and fingerlings of different types of fishes such as catla (Catla catla), rohu (Labeo rohita), mrigal (Cirrhina mrigala), grass carp (Ctenophmyngodon idella), bighead (Aristichthys nobili), silver carp (Hypophthalmichthys molitrix), common carp (Cyprinus cmpio) and rajpunti (Puntius gonionatus) @ 500/acre were stocked. Feed containing 25% protein was used two times daily and feed was adjusted fortnightly. After 8 months, all the fishes were weighed 0.80-2.10 kg except rajpunti (150-200 g) and tilapia (150-220 g), and a total of 25 ton of fish was harvested which was five times higher than the previous production under signal ownership. The production of fishes were increased after partitioning the lake with dikes due to proper management and control.

Studies on the growth and production of six major and exotic carps in Nasti baor, Jhenaidah, Bangladesh

An investigation on growth, production and fishery of three Indian major carps: rohu, Labeo rohita, catla, Catla catla and mrigal, Cirrhinus mrigala and three exotic carps: silver carp, Hypophthalmichthys molitrix, grass carp, Ctenopharyngodon idella, and common carp, Cyprinus carpio was carried out in Nasti baor during February to April months. In catch per unit effort (CPUE) study the highest catch/day/person (3.13 kg) and catch/day/gear (40.65 kg) was recorded in the month of March for kochal fishing. In komar fishing catch/day/person (15.08 kg) and catch/day/gear (1206 kg) was also found higher in March. Komar fishing was done only in March and April and its CPUE was greater in both the months than that of kochal. The average recovery rate (combination of all six species) was 37.80 considering the stocking from July month of the previous year. The recovery rate of common carp (54.1) was the highest and lowest (13.90) in case of silver carp. When the recovery was calculated on the basis of one year data and stocking, it was 55.6%. Analysis of production model revealed that the present production (54,806 kg/year) is less than both theoretical production (model I- 85,285 kg/year and model II -75,952 kg/year) estimated. Therefore, it may be concluded that the fish production from Nasti baor could still be increased from the present level of production.

Role of Aeromonas hydrophila in bacterial septicemia of cultured carps in Khouzestan Province and investigation on protectivity of bacterin (s) prepared from acute isolate(s) of it

Motile Aeromonas are the most common bacteria of freshwater in the world that cause disease in fish and other cold-blooded and warm-blooded hosts. Among this group of bacteria, Aeromonas hydrophila is important in causing complications such as fin rot, skin ulcers and lethal hemorrhagic septicemia in fish. Several virulence factors involved in the pathogenesis of Aeromonas hydrophila, including extracellular enzymes (protease, lipase, elastase, gelatinase and nuclease) and toxins. From the exotoxins, hemolysin, aerolysin and cytolytic enterotoxin play an important role in pathogenesis. Detection of virulence markers by PCR as a key component of determining the pathogenesis of the bacteria and using indigenous vaccines for better immunization against this disease is important. In this study, a total of 200 fanned carps (126 common carp. 39 silver carp and 35 of grass carp) with symptoms suspected aeromonas septicemia were isolated from Khouzestan province farms. 125 bacteria belong to Aeromonas genus detected by biochemical and PCR methods. 31 of all isolates recognized as Aeromonas hydrophila with biochemical methods, I6srRNA detection and Lipase genes. Results showed that the role of Aeromonas sp. and Aeromonas hydrophila in fish with disease symptoms were 62.5% and 15.5% respectively. By using specific primers, three virulence genes including hemolysin, aerolysine and cytolytic enterotoxin were detected in these confirmed isolates, that 18 isolates (58/06%) hemolysin positive (hlyA +), 16 isolates (51/61%) aerolysine positive (aerA+) and 23 isolates (74/19%) for cytolytic enterotoxin gene (act+) were positive. The result of present study showed that most of the confirmed isolates genotype was hlyA+ act- with frequency equal to 51/61%. For investigating the protection effect of acut strain of bacteria, UV inactivated bacterin was used.

Spawn production of common carp

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Lessons from the breeding program on common carp in Hungary

Common carp is one of the most important cultured freshwater fish species in the world. Its production in freshwater areas is the second largest in Europe after rainbow trout. Common carp production in Europe was 146,845 t in 2004 (FAO Fishstat Plus 2006). Common carp production is concentrated mainly in Central and Eastern Europe. In Hungary, common carp has been traditionally cultured in earthen ponds since the late 19th century, following the sharp drop in catches from natural waters, due to the regulation of main river systems. Different production technologies and unintentional selection methods resulted in a wide variety of this species. Just before the intensification of rearing technology and the exchange of stocking materials among fish farms (early sixties), “landraces” of carp were collected from practically all Hungarian fish farms into a live gene bank at the Research Institute for Fisheries, Aquaculture and Irrigation (HAKI) at Szarvas (Bakos and Gorda 1995; Bakos and Gorda 2001). In order to provide highly productive hybrids for production purposes starting from 1964, different strains and crosses between Hungarian landraces were created and tested. During the last 40 years, approximately 150 two-, three-, and four-line hybrids were produced. While developing parental lines, methods of individual selection, inbreeding, backcrossing of lines, gynogenesis and sex reversal were used. This breeding program resulted in three outstanding hybrids: “Szarvas 215 mirror” and “Szarvas P31 scaly” for pond production, and “Szarvas P34 scaly” for angling waters. Besides satisfying the needs of industry, the live gene bank helped to conserve the biological diversity of Hungarian carp landraces. Fifteen Hungarian carp landraces are still maintained today in the gene bank. Through exchange programs fifteen foreign carp strains were added to the collection from Central and Eastern Europe, as well as Southeast Asia (Bakos and Gorda 2001). Besides developing the methodology to maintain live specimens in the gene bank, the National Carp Breeding Program has been initiated in cooperation with all the key stakeholders in Hungary, namely the National Association of Fish Producers (HOSZ), the National Institute for Agricultural Quality Control (OMMI), and the Research Institute for Fisheries, Aquaculture and Irrigation (HAKI). In addition, methodologies or technologies for broodstock management and carp performance testing have been developed. This National Carp Breeding Program is being implemented successfully since the mid-1990s.

Induced spawning of African cat fish Clarias lazera (C. and V.) using acetone dried carp pituitary extract, deoxy-corticosterone acetate and human chorionic gonadotropin

A study was conducted to determine the efficacy of carp pituitary extract, deoxycorticosterone acetate, and human chorionic gonadotropin in inducing spawning in Clarias lazera . Results indicate deoxycorticosterone acetate to be more potent than pituitary extract, although the difference is not significant

Investment prospects in the production of the common carp (Cyprinus carpio) fingerlings in freshwater ponds

Since the introduction of Common carp Cyprinus carpio in Oyo State (Nigeria) from Israel in 1964, various local breeding methods have been employed in carp rearing to improve the survival rate at all stages of development during breeding. The physico-chemical parameters of the ponds which were simultaneously investigated for carp rearing in this study includes temperature (t), dissolved oxygen (DO) and hydrogen-ion concentration (pH). However, high rates of water displacement in the breeding ponds were unfavourable to the development of zooplankton which play important role in the food web of C. carpio. The survival rates of 15.88-69.50% and 19.60-33.83% obtained for the egg-hatchling and hatchling-fingerling stages respectively were encouraging. A breeding performance of this magnitude was found to be viable, breaking even in the fourth year. However, an increase in size of this trial project would be more profitable and increase fingerling supply as well as provide employment opportunities. This study thereby provides some baseline information on some local techniques and progress in the propagation of C. carpio and scope for further improvement