Nursery rearing of Macrobrachium rosenbergii (de Man) using hapa-nets: effects of stocking density

An experiment was conducted in two phases for 45 days each to study the effects of six stocking densities (phase-I: 100, 200 and 300 PL/m² and phase-II: 250, 500 and 750 PL/m²) on growth and survival of Macrobrachium rosenbergii postlarvae (PL) in nylon hapa-nets (1.8m x 1.8m x 1.4m) installed in a pond. Stocking densities of 100, 200 and 300 PL 10/m² resulted in similar (P<0.05) body length (47-48 mm) and survival rate (84-88%), while body weight (0.62g) in PL with 300/m² was significantly lower than that (0.70g) in PL with 100 and 200/m². The growth (body length 47mm and weight 0.64g) and survival (84%) of PL stocked at 250 PL/m² density were significantly higher (P<0.05) than that of PL at 500 and 750/m². Besides the variation in growth and survival in PL at six tests stocking densities, a sharp increase in body weight of PL was observed beginning at the 4th week of rearing.

Investigation on bacterial flora of farmed freshwater prawn (Macrobrachium rosenbergii de Man) in Bangladesh

Total bacterial load in the haemolymph of freshwater prawn Macrobrachium rosenbergii varied from 6.2x10⁴ to 1.9x10⁷ CFU/ml whereas in the hepatopancreas, bacterial load varied from 1.9x10³ to 2.9x10⁵ CFU/g. The total bacterial load in the pond water varied from 2.6x10² to 4.1x10⁵ CFU/ml. The isolated bacterial genera in the haemolymph and the hepatopancreas of prawn were Streptococcus, Acinetobacter, Micrococcus, Aeromonas, Vibrio, Flavobacterium, Staphylococcus and Pseudomonas, whereas the detected bacterial genera in pond water were Micrococcus, Streptococcus, Vibrio, Flavobacterium, Staphylococcus, Pseudomonas and Aeromonas. Among the detected genera, Vibrio and Staphylococcus were found to be dominant genera in the haemolymph of the sampled prawn throughout the study period whereas Staphylococcus and Pseudomonas were dominant in pond water.

Development of low-cost feed for culture of giant freshwater prawn (Macrobrachium rosenbergii de Man) in ponds

An experiment was conducted for 105 days in 12 earthen mini ponds of each 30m² size. Five different experimental diets containing 32% protein were formulated and prepared using fishmeal, shrimp meal, soybean meal, mustard oil cake, sesame meal, wheat bran and rice bran. A commercial shrimp diet (SABINCO starter-III) was assigned to treatment six and considered as the control. Prawns were stocked at the rate of 2.5 fry/m² and feed twice daily at the rate of 10% at the beginning and reduced to 8% for the last two months. The results of the experiment showed that prawn fed diets 1, 2, and 6 (control) showed significantly (P<0.05) highest weight gain among the dietary groups, while prawn fed diet 5 showed significantly lowest weight gain. The FCR values of diets ranged between 3.06 to 4.85. Prawns fed diet 1 and 6 showed significantly higher SGR, survival (%) and production among the dietary groups. The survival (%) of the prawns ranged between 46.6 to 66.6% and the production ranged between 304.5 to 563.3 kg/ha/105 days. The result of the study showed that diet containing 30% fishmeal, 5% shrimp meal, 5% soybean meal, 10% mustard oil cake, 10% sesame meal, 20% wheat bran, 18% rice bran, 1% oyster shell and 1% vitamin premix may be recommended for monoculture of M. rosenbergii.

Distribution of Macrobrachium rosenbergii (de Man) in three rivers of Paikgacha, Bangladesh

Distribution of post larvae, juveniles and adults of Macrobrachium rosenbergii was studied in three rivers of Paikgachha, Khulna during November '91 to Apri1 '94. The adults were found to ascend upstream and the matured ones again return to the estuary to breed. Post larvae of M. rosenbergii prefer a salinity range in between 0.5 to 19.0 ppt and juveniles require a salinity range in between 0.0 to 2.0 ppt.

Post-larval production of Macrobrachium rosenbergii using mass-cultured live food organisms

Macrobrachium rosenbergii post-larvae were produced in 1992 and 1993 using Artemia nauplii and cultured zooplankton Brachionus plicatilis (rotifier), Apocyclops dengizicus (copepod) and Moina sp. (cladoceran) supplemented with chopped Tubifex worms. In 1992 (first trial) two experiments were carried out under water temperature range of 24.5 to 28°C and 26.0 to 28.5 °C respectively and corresponding post-larval production was 5.6% and 86.3%. The duration of experiments was 58 and 40 days. During second trial in 1993 water temperature varied between 25.0 to 27.0°C. At the end of 59 days the post-larvae were found to be 44% of the total number of larvae stocked on the first day.

Breeding performance of Macrobrachium rosenbergii in captivity

The key factor in success of a Macrobrachium hatchery is to yield maximum number of larvae with minimum number of brooders. Suitable environmental conditions were provided and the breeding performance of females of M. rosenbergii was observed. A total number of 140 females brought from wild were used for this purpose. Each female was tagged and given a code number which made it possible to record the gestation period, interbreed period, number of breeding and larval yield of individual female during each breeding. Females were found to breed six times during the ten months duration. Of the total females studied, 99.12 % bred once, while only 2.16 % females bred six times in a total span of ten months. The average number of larvae decreased successively from first to fourth breeding. The average interbrood period was minimum between the first and second breeding and maximum between the fourth and fifth breeding. The highest average larval yield of 25,428 was recorded in the first breeding, the lowest of 4,596 in the fourth breeding. The possible reasons for variations in interbrood period and larval yield are discussed.

Substitution of Artemia with Brachionus in different ratios during larval rearing of Macrobrachium rosenbergii and evaluation of their feed efficacy

Larval growth during stage I-VIII was studied in Macrobrachium rosenbergii. Duration in moult periodicity were recorded-during larval development period, larvae were fed with Brachionus (grown on Baker's yeast and also Brachinous raised through organic manuring in outdoor culture containers). The performance of the feed was evaluated through substitution of Brachionus in the feeding protocol, in lieu of Artemia 1st instar. The Artemia, Brachionus substitution ratio of 75:25 was found to be most efficient. The study also indicates that the comparative growth rate of Brachionus plicatilis is higher in manure loaded tanks than with Baker's yeast. Growth rate "Y'' in culture tank being 0.245 and 0.112 and corresponding duplicating time (Td) too was found to be 2.855 and 6.365 respectively in tanks manured/enriched with pig manure.

Estimation of fishing mortality and migration coefficient of the fishery of juvenile Macrobrachium rosenbergii from Kalu River in Maharashtra

The freshwater prawn, Macrobrachium rosenbergii breeds in estuaries and the juveniles after completion of their larval stage start their upward migration towards rivers. It is at this stage fishing of juveniles takes place in river mouths. Kalu River near Titwala, in Maharashtra is estimated based on data presented by Indulkar and Shirgur (1995) for 1991 and 1992 fishing seasons. The fishing mortality was estimated to be 1.50 and 1.28 for a fishing season of 3 months in 1991 and 1992 respectively, while the migration coefficient was computed to be 3.53 during the fishing season. As the average exploitation rate during the study period was only 0.24, the juveniles are not heavily fished and there is a scope for almost doubling the present catch to about 4 million seeds per fishing season.

Ice storage characteristics of cultured Macrobrachium rosenbergii (de Man)

Cultured Macrobrachium rosenbergii (Scampi; 30-40 g) in headless shell-on form was stored in ice (Prawn:ice=1:2) in a plywood box insulated with 2 cm thick expanded polystyrene and subjected to detailed examination of quality by chemical, microbiological and organoleptic evaluation at regular intervals to assess the storage life. The prime quality life of prawns in ice was found to be 8 days followed by a phase of lowered quality, but still acceptable till the end of 13 days.

Frozen storage characteristics of cultured Macrobrachium rosenbergii (de Man)

Cultured Macrobrachium rosenbergii (Scampi, about 30 g each) in headless shell-on form was individually quick frozen in a spiral freezer. The frozen samples were glazed and packed in polythene bags, which were further packed in master carton and stored at -18°C. Samples were drawn at regular intervals and subjected to biochemical, bacteriological and organoleptic analysis to study its storage characteristics. The data on the above parameters showed that the samples were in prime acceptable condition when stored up to 23 weeks. No appreciable change in colour and odour was noticed in the raw muscle. Afterwards, organoleptic evaluation of the cooked muscle revealed slight change in the flavour. Texture also appeared little tougher. These changes in organoleptic characters were well supported by the biochemical bacteriological changes in the muscle.

Polyculture of giant freshwater prawn, Macrobrachium rosenbergii, with Indian major carps: a case study

A study conducted in a 450 m² earthen pond to evaluate the production potential of giant freshwater prawn (stocked at 20,000 juveniles/ha) and Indian major carps, catla and rohu (stocked at 5000 juveniles/ha in 2:1 proportion) revealed that in nine months growing period, catla and rohu attained average sizes of 357 and 746 g, respectively, while prawn weighed 48.32 g. The growth of rohu was much faster than catla as indicated by higher relative and absolute weight gains. The total fish production per hectare was estimated to be 2418 kg and prawn production stood at 780 kg with excellent survival of both the fish (>98%) and prawn (>80%).

Evaluation of some formulated diets for rearing the post-larvae of Macrobrachium rosenbergii

Macrobrachium rosenbergii post-larvae with an average weight of 6.26 ± 0.23 mg and an average length of 10.67 ± 0.13 mm were fed with one of the five experimental diets having graded levels of lipid (5.0, 7.5, 10.0, 12.5 and 15.0%) to satiation two times a day to study their effect on growth, survival and feed utilisation. The highest weight gain was observed in post-larvae fed 7.5% lipid, although there was no significant difference (p>0.05) between groups fed 7.5 and 10.0% lipid. Similar effect was observed in the specific growth rate and protein efficiency ratio of post-larvae fed the diet with 7.5 and 10.0% lipid. The lowest feed conversion ratio was obtained in postlarvae fed the diet with 7.5% lipid. The survival rates of post-larvae fed on various levels of dietary lipid differed significantly (p<0.05) after 15 days of rearing period. Significantly lower survival was observed in the diet with 15.0% lipid level. Dietary lipid did not significantly affect prawn carcass protein, lipid and ash contents. Based on the data analysis (ANOVA – one way analysis), the dietary lipid requirement for 15 days of nursery rearing of Macrobrachium rosenbergii post-larvae was estimated to be 7.5 to 10.0% under experimental conditions in this study.

Effects of physiological doses of vitamin D sub(3) (Cholecalciferol) on induced molting and growth in giant freshwater prawn, Macrobrachium rosenbergii (de Man)

Effects of three different doses of vitamin D sub(3) on molting, growth, and calcium and phosphate composition of tissue and molt during the grow-out of the giant freshwater prawn Macrobrachium rosenbergii (average weight 10.56 ± 0.20 g), obtained from a grow-out pond, were studied. Intramuscular doses of vitamin D sub(3) (100, 500 and 2000 IU/kg body weight) were given on the 1st, 3rd, 5th, 7th, 9th, 11th, 13th, 15th, 20th, 25th and 30th days. All the experimental animals were fed with a basal diet containing fish meal, shrimp meal, wheat flour, groundnut de-oiled cake, soybean meal and wheat bran at 3% of the body weight. The numbers of molts were recorded as 20±0.50, 29±1.16, 51±1.87, and 30±1.60 in control, 100, 500 and 2000 IU/kg body weight physiological doses, respectively. Maximum growth was recorded in prawns given 500 IU/ kg dose. Survival was between 58.33 ± 9.13 and 77.77 ± 8.61%. The ash content and calcium level increased significantly (p<0.05) and recorded the highest values in 500 IU/kg physiological dose. However, the inorganic phosphate (P sub(i)) content recorded the highest values in tissue in 2000 IU/kg dose (p<0.05, F = 50.60613). There is no significant difference in calcium contents (p>0.05) in both tissue and molt at 500 and 2000 IU/kg doses. It was found that a higher physiological dose (2000 IU/kg) of vitamin D sub(3) increased the rate of mortality. Results have shown that vitamin D sub(3) has a positive impact on the growth and survival of M. rosenbergii and it interferes with the metabolism of Ca and P sub(i), in tissue, and alters molting frequency. Results on physiological dose suggest an alternative and effective dietary supplementation method of vitamin D sub(3) in the grow-out phase of M. rosenbergii.

A record of Probopyrus bithynis (Richardson, 1904) in Macrobrachium rosenbergii (De Man) from coastal Andhra Pradesh, India, with special reference to host-parasite relationship

An incidence of bopyrid isopod infestation was observed in giant freshwater prawn, Macrobrachium rosenbergii (de Man) juveniles (40-60 mm/0.9-1.5 g) in a scampi culture farm in East Godavari district of Andhra Pradesh. The presence of parasite was observed by conspicuous boil like swelling of the branchial chamber where the parasite was found lodged on the gills. The infested gill was highly compressed and necrosed. Only one branchial chamber was infested by the parasite while the other gill was normal. The infested prawns were thin and emaciated and showed retarded growth. The parasite was identified as Probopyrus bithynis (Richardson, 1904) which caused inhibition of ventilation due to its permanent lodging in the branchial chamber and impaired the gaseous exchange by gills. It was also observed that this parasite caused parasitic castration in the infested prawns.

Use of crude salt in rearing of freshwater giant prawn, Macrobrachium rosenbergii (de Man) larvae

Three different types of culture media: (i) 100% brine (B 100 ), (ii) 75% brine and 25% crude salt (B 75 CS 25 ), and 50% brine and 50% crude salt (B 50 CS 50) were tested to evaluate the possible use of brackish water reconstituted from the crude salt for the production of M. rosenbergii post-larvae. The production rate of 25.26±0.20 PI/l with a corresponding survival rate of 84.20±0.66% was significantly higher (P<0.05) for the larvae reared on B100 than that of 22.10±0.57 Pl/l with a corresponding survival rate of 73.68±1.89% on B50CS50. Larvae cultured on B75CS25 did not show any significant difference (P<0.05) in production as well as in survival of post-larvae than that on B100. The result shows that, for rearing of prawn larvae, use of brine can be replaced up to 25% without any undue reduction in production of post-larvae. However, the production as well as survival rate of post-larvae with 50% replacement (B50CS50) is also appreciable. It is assumed that the mineral constituents of natural seawater might have some triggering effects on prawn larvae in closing their larval cycle.