Use of stereo camera systems for assessment of rockfish abundance in untrawlable areas and for recording pollock behavior during midwater trawls

We describe the application of two types of stereo camera systems in fisheries research, including the design, calibration, analysis techniques, and precision of the data obtained with these systems. The first is a stereo video system deployed by using a quick-responding winch with a live feed to provide species- and size- composition data adequate to produce acoustically based biomass estimates of rockfish. This system was tested on the eastern Bering Sea slope where rockfish were measured. Rockfish sizes were similar to those sampled with a bottom trawl and the relative error in multiple measurements of the same rockfish in multiple still-frame images was small. Measurement errors of up to 5.5% were found on a calibration target of known size. The second system consisted of a pair of still-image digital cameras mounted inside a midwater trawl. Processing of the stereo images allowed fish length, fish orientation in relation to the camera platform, and relative distance of the fish to the trawl netting to be determined. The video system was useful for surveying fish in Alaska, but it could also be used broadly in other situations where it is difficult to obtain species-composition or size-composition information. Likewise, the still-image system could be used for fisheries research to obtain data on size, position, and orientation of fish.

Statistical inference about the relative efficiency of a new survey protocol, based on paired-tow survey calibration data

Paired-tow calibration studies provide information on changes in survey catchability that may occur because of some necessary change in protocols (e.g., change in vessel or vessel gear) in a fish stock survey. This information is important to ensure the continuity of annual time-series of survey indices of stock size that provide the basis for fish stock assessments. There are several statistical models used to analyze the paired-catch data from calibration studies. Our main contributions are results from simulation experiments designed to measure the accuracy of statistical inferences derived from some of these models. Our results show that a model commonly used to analyze calibration data can provide unreliable statistical results when there is between-tow spatial variation in the stock densities at each paired-tow site. However, a generalized linear mixed-effects model gave very reliable results over a wide range of spatial variations in densities and we recommend it for the analysis of paired-tow survey calibration data. This conclusion also applies if there is between-tow variation in catchability.

Comparison of echogram measurements against data expectations and assumptions for distinguishing seafloor substrates

Defining types of seafloor substrate and relating them to the distribution of fish and invertebrates is an important but difficult goal. An examination of the processing steps of a commercial acoustics analyzing software program, as well as the data values produced by the proprietary first echo measurements, revealed potential benef its and drawbacks for distinguishing acoustically distinct seafloor substrates. The positive aspects were convenient processing steps such as gain adjustment, accurate bottom picking, ease of bad data exclusion, and the ability to average across successive pings in order to increase the signal-to-noise ratio. A noteworthy drawback with the processing was the potential for accidental inclusion of a second echo as if it were part of the first echo. Detailed examination of the echogram measurements quantified the amount of collinearity, revealed the lack of standardization (subtraction of mean, division by standard deviation) before principal components analysis (PCA), and showed correlations of individual echogram measurements with depth and seafloor slope. Despite the facility of the software, these previously unknown processing pitfalls and echogram measurement characteristics may have created data artifacts that generated user-derived substrate classifications, rather than actual seafloor substrate types.

Measurements of total scattering spectra from bocaccio (Sebastes paucispinis)

Marine sportfishing in southern California is a huge industry with annual revenues totaling many billions of dollars. However, the stocks of lingcod and six rockfish species have been declared overfished by the Pacific Fisheries Management Council. As part of a multifaceted fisheries management plan, marine conservation areas, covering many million square nautical miles, have been mandated. To monitor the recovery of the rockfish stocks in these areas, scientists are faced with the following challenges: 1) multiple species of rockfish exist in these areas; 2) the species reside near or on the bottom at depths of 80 to 300 m; and 3) they are low in numerical density. To meet these challenges, multifrequency echosounders, multibeam sonar, and cameras mounted on remotely operated vehicles are frequently used (Reynolds et al., 2001). The accuracy and precision of these echosounder results are largely dependent upon the accuracy of the species classification and target strength estimation (MacLennan and Simmonds, 1992).

Precision and accuracy of fish length measurements obtained with two visual underwater methods

During the VITAL cruise in the Bay of Biscay in summer 2002, two devices for measuring the length of swimming fish were tested: 1) a mechanical crown that emitted a pair of parallel laser beams and that was mounted on the main camera and 2) an underwater auto-focus video camera. The precision and accuracy of these devices were compared and the various sources of measurement errors were estimated by repeatedly measuring fixed and mobile objects and live fish. It was found that fish mobility is the main source of error for these devices because they require that the objects to be measured are perpendicular to the field of vision. The best performance was obtained with the laser method where a video-replay of laser spots (projected on fish bodies) carrying real-time size information was used. The auto-focus system performed poorly because of a delay in obtaining focus and because of some technical problems.

Using bone measurements to estimate the original sizes of bluefish (Pomatomus saltatrix) from digested remains

The ability to estimate the original size of an ingested prey item is an important step in understanding the community and population structure of piscivorous predators (Scharf et al., 1998). More specifically, knowledge of original prey size is essential for deriving important biological information, such as predator consumption rates, biomass of the prey consumed, and selectivity of a predator towards a specific size class of prey (Hansel et al., 1988; Scharf et al., 1997; Radke et al., 2000). To accurately assess the overall “top-down” pressure a predator may exert on prey community structure, prey size is crucial. However, such information is often difficult to collect in the field (Trippel and Beamish, 1987). Stomach-content analyses are the most common methods for examining the diets of piscivorous fish, but the prey items found are often thoroughly digested and sometimes unidentifiable. As a result, obtaining a direct measurement of prey items is frequently impossible.

Using measurements of muscle cell nuclear RNA with flow cytometry to improve assessment of larval condition of Walleye Pollock (Gadus chalcogrammus)

Nuclear RNA and DNA in muscle cell nuclei of laboratory-reared larvae of Walleye Pollock (Gadus chalcogrammus) were simultaneously measured through the use of flow cytometry for cell-cycle analysis during 2009–11. The addition of nuclear RNA as a covariate increased by 4% the classification accuracy of a discriminant analysis model that used cell-cycle, temperature, and standard length to measure larval condition, compared with a model without it. The greatest improvement, a 7% increase in accuracy, was observed for small larvae (<6.00 mm). Nuclear RNA content varied with rearing temperature, increasing as temperature decreased. There was a loss of DNA when larvae were frozen and thawed because the percentage of cells in the DNA synthesis cell-cycle phase decreased, but DNA content was stable during storage of frozen tissue.

Atmospheric chlorofluoromethanes as tracers of ocean circulation and mixing: measurement and calibration techniques and studies in the Greenland and Norwegian seas

EXTRACT (SEE PDF FOR FULL ABSTRACT): An analytical system was designed and constructed for the rapid and accurate shipboard measurement of anthropogenic chlorofluoromethanes in seawater and in air, using electron capture gas chrometography. The distribution of these compounds in the marine atmosphere and the water column in the Greenland and Norwegian seas were studied during February and March, 1982. The compounds, dissolved in the ocean from the atmosphere, can be used as tracers of subsurface ocean circulation and mixing processes.

Trawling pull exerted by a trawler: a method of estimation from propeller dimensions and its comparison with sea measurements

The proper matching of the pull exerted by a trawler and the size of trawl is important for maximizing the catching efficiency. The available pull is more dependent on the propeller and its working conditions than the installed engine power. The normal practice is to directly connect net size to the installed power in the boat by formulae without reference to the prope1ler dimensions or the available trawling pull and this is not adequate to find out the optimum combination. By the method outlined in this paper, the accurate calculation of trawling pull is possible by taking into account only the propeller diameter, pitch and r. p. m. The predictions by the method are compared for trawlers with powers between 30 and 60 hp and agreement is found to be within + 5%. The power absorbed by the propeller in trawling condition can also be calculated by this method for checking whether the engine is being overloaded.

Wadge Bank trawl fishery studies. Pt. 4. An analysis of the length frequency measurements of the sea bream (Lethrinus nebulosus) made in 1949 and 1953 to 1958

Length frequency distributions of the sea bream collected during the period 1953 to 1958 have been analysed. The increase in average sizes of the sea bream with depth suggests a movement to deeper waters with increase in size. By numbers, the sea bream is more abundant between 21 and 30 fathoms than in deeper areas. The recruitment was continuous and regular. There is no sign of entry or progression of a dominant brood throughout the period under study. Length frequency distribution shows three distinct modes. The first mode occurs regularly but does not progress beyond 40cm, recruitment being balanced by natural and fishing mortality. The other two which are not regular are probably the result of fishing outside regular areas. Short sections of “growth” lines which fit into one another when extrapolated, are evident. The larger lines obtained by extrapolation are parallel to one another. These tentative "growth lines" indicate that this species which enters the fishing grounds, when 15 cm or larger in length are exploited by the trawl fishery for a period of three to four years. This species appears to be six months old when it enters the fishing grounds and increases in length by about 37.5 cm in the next 30 months. Later growth slows down. The average size of the specimens sampled continued to get smaller from 1953 till 1957. It is shown that this reduction in size is due to increased fishing effort.

Salinity measurements and use of the Practical Salinity Scale (PSS)

Salinity, temperature and pressure are parameters which govern the oceanographic state of a marine water body and together they make up density of seawater. In this contribution we will focus our interest on one of these parameters, the salinity: accuracy in relation to different purposes as well as observation technique and instrumentation. We will also discuss the definition of salinity. For example most of the Indian Ocean waters are within the salinity range from 34.60-34.80, which emphasize the importance of careful observations and clear definitions of salinity, in such a way that it is possible to define water masses and predict their movements. In coastal waters the salinity usually features much larger variation in time and space and thus less accuracy is sometimes needed. Salinity has been measured and defined in several ways over the past century. While early measurements were based on the amount of salt in a sea water sample, today the salinity of seawater is most often determined from its conductivity. As conductivity is a function of salinity and temperature, determination involves also measurement of the density of seawater is now more precisely estimated and thus the temperature. As a result of this method the Practical Salinity Scale (PSS) was developed. The best determination of salinity from conductivity and the temperature measurements gives salinity with resolution of 0.001 psu, while the accuracy of titration method was about ± 0.02‰. Because of that, even calculation of movements in the ocean is also improved.

Morphometric measurements as an index for estimating yield of meat in shell fishes, 2. Mussel (Perna viridis) and clam (villorita cyprinoides)

Observations (76 nos) on height-length and whole weight-meat weight relations of mussels (Perna viridis), both wild and cultured were made. From the length of mussel the height can be worked out by the equations (logarithmic scale), 1. y = 0.360+0.988 x for wild; 2. y = 0.334+1.011 x for cultured, where x is the length (cm) and y is the height (cms). So also to any height the corresponding meat weight can be obtained by the regression equation. log w=-0.8178+1.9769 log H for wild variety (1) log w=-1.3049+2.8385 log H for culture-variety (2) where w is the meat weight (g) and H is the height (cm) of the mussel. Fourteen observations on size weight measurements of dams were made. The yield varied from 8.9 to 13%. The length-height relationship worked out for clams (Villorita sp) is y=0.485+1.005 x for length x and height y.

Morphometric measurements as an index for estimating yield of meat of shell fishes, 1. Crab (Scylla serrata) Forskal

Sixty one observations on length-breadth and whole weight-meat weight relations of India crab (Scylla serrata) were made. From the length of crab (cm) the whole weight (gm) can be computed by the equation: log W=-0.1708+2.3341 log L. Similarly for any given length (cm) the meat weight (gm) can be found by the relation, log w=-1.5745+3.0148 log L.