Studies on fecundity, sex ratio and crossbreeding in different strains of Guppy

Studies on the fecundity, sex ratio and crossbreeding in different strains of the guppy Poecilia reticulata such as Butterfly I, Butterfly II and Cobra strains was carried out. The highest fecundity among Butterfly I strain was at the 5th breed while it was highest at the 6th and 8th breed among Butterfly II and Cobra strain respectively. The average male percentage was 39.88%, 43.39% and 45.02% among Butterfly I, Butterfly II and Cobra strains respectively. The crossbreeding of strains showed good results among crossbreeds of Butterfly II (♀) X Cobra (♂) and Butterfly I (♀) X Cobra (♂). The crossbreed among the Butterfly I (♀) X Cobra (♂) proved to be excellent from the point of view of more percentage, rapid growth, and brilliant body and finnage colouration.

Breeding performance of Macrobrachium rosenbergii in captivity

The key factor in success of a Macrobrachium hatchery is to yield maximum number of larvae with minimum number of brooders. Suitable environmental conditions were provided and the breeding performance of females of M. rosenbergii was observed. A total number of 140 females brought from wild were used for this purpose. Each female was tagged and given a code number which made it possible to record the gestation period, interbreed period, number of breeding and larval yield of individual female during each breeding. Females were found to breed six times during the ten months duration. Of the total females studied, 99.12 % bred once, while only 2.16 % females bred six times in a total span of ten months. The average number of larvae decreased successively from first to fourth breeding. The average interbrood period was minimum between the first and second breeding and maximum between the fourth and fifth breeding. The highest average larval yield of 25,428 was recorded in the first breeding, the lowest of 4,596 in the fourth breeding. The possible reasons for variations in interbrood period and larval yield are discussed.

Reproductive biology, artificial propagation and larval rearing of two freshwater eels, Monopterus cuchia and Mastacembelus armatus

Studies on reproductive biology and artificial propagation including larval rearing of freshwater mud eel, Monopterus cuchia and spiny eel, Mastacembelus armatus were attempted. The gonadosomatic index (GSI) of mud eel ranged from 0.41 (August) to 5.52 (June) in males and 0.53 (August) to 7.61 (June) in females. In both cases the GSI showed a peak in June. Fecundity ranged from 228 (TL - 396 mm; W - 78g) to 5510 (TL - 865 mm; W - 630 g). In case of spiny eel, the GSI varied from 0.65 (August) to 8.30 (July) in males and 0.70 (August) to 10.46 (July) in females. GSI showed single peak in July. Fecundity ranged from 570 (TL - 240 mm; W - 30 g) to 10870 (TL - 601; W - 350g). Histology of the testes and ovaries of the eels were carried out to investigate the gonadal development stages during the reproductive months (August to November 2003). In case of male M. cuchia, the secondary primordial germ cells, primary spermatogonium, some spermatogonia A and clone of spermatogonium B in testis were observed in September. In October-males different sized lobules having spermatogonia, spermatocytes and spermatids were observed. In the ovary of M. cuchia, polygonal shaped oocytes were seen during September. The oogonia were reduced with dense and irregular shaped during October. Numerous pycnotic cells were visible during November. In male M. armatus numerous broken lobule walls were found in testes during September. In October, abundant primary germ cells, pycnotic nests of degenerating cells, spermatogonia and spermatids were observed. In females, ovaries had distinct yolk vesicles stage and yolk granules stages in August. In September, the follicular cells of the oogonia were ruptured, shrunk forming irregular shaped in October. Oogonia were also shrunk with thin, irregular shaped structure but broken parts of the ruptured follicular cells were scattered in case of M. armatus. Experimental attempts on artificial propagation indicated that both freshwater eels were difficult to breed using inducing agents like pituitary glands (PG) of 10, 20, 50, 100 and 150 mg per kg of body weight. Same doses were used for both sexes with equal sex-ratio. In both cases, brood fish died at higher doses of injection given at 100 and 150 mg PG/kg bodyweight. However, M. cuchia breed naturally in cisterns when provided with water hyacinths and tunnel in muddy bottom. M. cuchia fed with chopped cooked fish attained a mean weight of 18.75 ± 2.3 g and cent percent survival. While in case of M. armatus best growth by weight (12.0 ± 2.48 g) and cent percent survival were achieved using chopped raw fish. Car tyre was observed as best shelter for attaining the mean weight gain 22.53 ± 2.24 g and cent percent survival of M. cuchia. While PVC pipe was found to be the best shelter for M. armatus, where it attained the mean weight of 12.73 ± 1.88 g and cent percent survival.

Maturity, spawning and fecundity of Saurida tumbil (Bloch, 1795) from Mumbai waters

The absolute fecundity of Saurida tumbil ranged between 24160 to 172000 eggs with weight range of 230-670 g. Linear relationship between fecundity and weight was more valid than that of length and ovary weight. The fish breed once a year during November to March. The length at which 50% of the females mature was calculated as 296 mm. The female dominated the commercial catch and overall male to female ratio of population was 1:1.62 throughout the year.

Preliminary success on hormone induced captive breeding of goldspot mullet, Liza parsia (Ham.)

An attempt was made to breed goldspot mullet, Liza parsia in captivity through hormone induction. The fish started spawning 35-36 hours after a single dose of 2ml ova prim per kg body weight. Hatching of fertilized eggs completed within 42-48 hours after spawning. The mean hatching rate (%) was 71.33±12 corresponding to the fertilization rate (%) of 64±12. The larvae started its first external feeding on the third day and attained a length 2.5±0.25 mm. The salinity of both breeding and rearing cisterns was 20‰ and temperature was maintained at 22-23°C.

Effects of dietary vitamin E on the growth and breeding performance of Ompok pabda

A 90 day feeding trail was conducted to investigate the effect of vitamin E on the growth and breeding performance of Ompok pabda. A total of 84 healthy female brood fish (41.10±0.44g) were divided into 4 treatments i.e. treatment T1 T2, T3 and T4 having three replications each. The fish were fed twice a day with a standard feed (40% protein) having 4 doses of vitamin E viz. 0 (served as control), 50, 100 and 150mg vitamin E/kg feed. At the end of the feeding trial, the brood fish were induced to breed with PG extract to observe the effect of vitamin Eon feed. After rearing for 90 days with the experimental feeds, it was found that weight gain and specific growth rate of brood fish fed with 100mg vitamin E/kg feed (treatment T3) was the highest (14.78±0.38g and 2.99±0.11) while 150mg vitamin E/kg feed (treatment T4) fed fish gave the poorest result (2.97±0.89g and 1.21±0.32). There was no significant difference in terms of length gain of brood fish among the different treatments. The brood fish were induced to breed with equal dose of PG extract (18 and 12mg PG/kg body weight for female and male respectively) to observe the dietary effect of vitamin E on breeding performance. The highest ovulation, fertilization and hatching rate of eggs were found to be 81.48±6.41%, 84.04±3.53% and 68.59±5.03% respectively in the brood fish of treatment T1 while the poorest (33.33±00%, 52.35±5.02% and 45.70±7.24% respectively) were found in the brood fish under treatment T4. The results suggest that inclusion of 100mg vitamin E/kg feed is best for enhancing the breeding performance of O. pabda brood fish indicating that vitamin E content has a positive impact on reproduction of fish. The present results also imply that inclusion of higher level of vitamin E exerts an antagonistic effect in terms of growth and breeding performance of this species.

A study of Rastrineobola argentea in the Ugandan lakes

Increased stocks of mukene Rastrineobola argentea and the subsequent interest in its fishery on Lake Victoria has been attributed to the poor performance of the endemic fishery as a result of introductions into this lake of foreign fish species Lates niloticus and Oreochromis niloticus. R. argentea now remains the only endemic fish species of economic importance ranking second to the Nile perch in this lake. Despite this importance, biological information on the species and knowledge of its fishery is scanty. Preliminary observations on the species in the Ugandan waters indicate that R. argentea feeds mainly on zooplankton (copepods) during daylight hours. Small quantities of aquatic insect larvae/pupae (chironomids and chaoborids) are also eaten mainly at night. These fishes breed just after the rainy seasons and the young eventually mature at between 43-44 mm standard length. Growth and population parameters show a rate of growth (K) of 0.92 with L of 64.5 mm S.L. Natural mortality (M) is given at 2.371 and total mortality (2) of 3.594. Two mesh size nets 10 and 5 mm are in use in the lake. The smaller mesh size which is more preferred by the artisanal fishermen however tends to capture many immature fishes. There is therefore need for a unified lakewide data collection on the species and its fishery in order to obtain more reliable biological information necessary proper management of this fast developing fishery.

Aquaculture potential of ornamental fishes of Uganda

Preliminary studies undertaken to investigate the availability of ornamental fish species in Uganda’s natural water systems, revealed significant abundance of coloured fishes in Uganda’s water systems including the Kyoga and Victoria Lake system. These species are able to breed in captivity and to feed on artificial diets in ponds and glass tanks. The species are attractive and are highly marketable. These observations indicate the potential to culture ornamental fishes as away of diversifying the range of aquaculture species, a means to generate income and to improve livelihoods in Uganda.

Relationship between the water levels and the fish catches in lakes Mweru and Mweru wa Ntipa, Zambia

The relationships between water level and catch per effort in two Zambian lakes are compared. In the relatively stable Lake Mweru, a positive correlation exists which can be used, with certain reservations, to predict the state of the fishery two years in advance. The cause of the relationship is probably the effect of water level on the marshy and swampy breeding areas, where at least the most common species in the commercial catch (Tilapia macruchir) has definite limits for the depth of water in which it will breed. For Mweru wa Ntipa, a consistant definite relationship does not exist, probably because the water level and extent of the lake fluctuate widely.