Effects of supplementary feeds on growth and survival of freshwater giant prawn (Macrobrachium resenbergii [i.e. rosenbergii] deMan)

Highest growth of prawn was obtained with Feed B (743 kg/ha) with highest survival rate (60.88%) followed by Feed A where production and survival rate was 659 kg/ha and 53.50%, respectively. Feed A contained 30% dry ground cow viscera, 40% oil cake, 20% rice-bran and 10% heat bran. Feed conversion ratios were found to be 7.60:1 for Feed A and 6.46:1 for Feed B, which indicated that Feed B was more efficiently utilized by the prawn than Feed A. Statistical analysis revealed that the differences in production of prawns among the treatments were highly significant (P< 0.01).

Production of Thai sharpunti (Puntius gonionotus Bleeker) in polyculture with carps using low-cost feed

The effectiveness of duckweed and rice bran as a low cost supplementary feed was compared through a six months production trial of rajpunti (Barbodes gonionotus) with carps. Six earthen ponds of 360 m² each were used for the trial. Three ponds received duckweed, while the other three received rice bran as supplementary feed. Fish biomass after six months of rearing increased to an average of 2,056 kg/ha in ponds which received duckweed and 2,056 kg/ha in rice bran treated ponds. The net profit with duckweed and rice bran worked out to Tk. 69.752 and Tk. 73.480 kg/ha, respectively. This study revealed that duckweed is a low cost supplementary feed, particularly for farmers with limited income.

Effects of artificial feeds on production of fishes in polyculture

A study on the effects of artificial feeds on the growth and production of fishes in polyculture in 6 ponds along with some limnological conditions was conducted. Species of Indian and Chinese major carps (Labeo rohita, Catla catla, Cirrhinus mrigala, Hypophthalmicthys molitrix) and catfishes (Clarias batrachus, Clarias gariepinus) were stocked in 6 ponds. Stocking rate in both cases were 32044 fingerlings per hectare. Ratio of species of Rui:Catla:Mrigal:Silver carp:African Magur:Local Magur=25%:25%:5%:25%:14%:6%. Fertilization and artificial feeds were given in 3 ponds (treatment I) and only fertilization was done in other 3 ponds (treatment II). Average yield/ha/yr was 7.903 m.ton in case of fertilization and artificial feeding application and 3.374 m.ton in case of only fertilization application. Urea, TSP and cow dung were applied fortnightly at the rates of 400 kg/ha/yr, 2000 kg/ha/yr and 4000 kg/ha/yr respectively. Wheat bran, rice bran and mustard oil cake were given daily as an artificial feed in treatment I. Whereas treatment II was conducted without any artificial feed. Ratio of artificial feed was wheat bran:rice bran:oil cake=2:2:1 (by wt). Absence of artificial feed in 3 ponds under treatment II seriously affected the growth and production of fish.

Development of low-cost feed for culture of giant freshwater prawn (Macrobrachium rosenbergii de Man) in ponds

An experiment was conducted for 105 days in 12 earthen mini ponds of each 30m² size. Five different experimental diets containing 32% protein were formulated and prepared using fishmeal, shrimp meal, soybean meal, mustard oil cake, sesame meal, wheat bran and rice bran. A commercial shrimp diet (SABINCO starter-III) was assigned to treatment six and considered as the control. Prawns were stocked at the rate of 2.5 fry/m² and feed twice daily at the rate of 10% at the beginning and reduced to 8% for the last two months. The results of the experiment showed that prawn fed diets 1, 2, and 6 (control) showed significantly (P<0.05) highest weight gain among the dietary groups, while prawn fed diet 5 showed significantly lowest weight gain. The FCR values of diets ranged between 3.06 to 4.85. Prawns fed diet 1 and 6 showed significantly higher SGR, survival (%) and production among the dietary groups. The survival (%) of the prawns ranged between 46.6 to 66.6% and the production ranged between 304.5 to 563.3 kg/ha/105 days. The result of the study showed that diet containing 30% fishmeal, 5% shrimp meal, 5% soybean meal, 10% mustard oil cake, 10% sesame meal, 20% wheat bran, 18% rice bran, 1% oyster shell and 1% vitamin premix may be recommended for monoculture of M. rosenbergii.

On continued culture trials of Lates calcarifer (Bloch) and Oreochromis mossambicus in the ponds of Kharland Research Station, Panvel, Maharashtra

Pond culture trials of Lates calcarifer (Bloch) were initiated in the ponds of Kharland Research Station, Panvel, from 1985 up to 1989 by adopting varying methods of inputs of commonly available organic substances comprising de-oiled cake of groundnut, rice bran and raw cattle dung. Although provision of zooplankton generated under phased fertilisation technique resulted in considerable yield level, subsequent trials in combination with direct feed methods gave higher yield. Trials during 1990-91 adopting similar feed input techniques were continued but by stocking the seed of both Lates and Oreochromis mossambicus with yield percentage around 72.23 for Lates and 27.73 for Oreochromis. Trials were further continued during next three years. In relation to the organic input levels the yields rate for seven months period was in the range of 119.4 kg/ha to 250.4 kg/ha for Lates. In case of Oreochromis the yield rate varied between 28.5 to 153.32 kg/ha indicating the influence of differential stocking rates and size of the seed of two species.

Effects of physiological doses of vitamin D sub(3) (Cholecalciferol) on induced molting and growth in giant freshwater prawn, Macrobrachium rosenbergii (de Man)

Effects of three different doses of vitamin D sub(3) on molting, growth, and calcium and phosphate composition of tissue and molt during the grow-out of the giant freshwater prawn Macrobrachium rosenbergii (average weight 10.56 ± 0.20 g), obtained from a grow-out pond, were studied. Intramuscular doses of vitamin D sub(3) (100, 500 and 2000 IU/kg body weight) were given on the 1st, 3rd, 5th, 7th, 9th, 11th, 13th, 15th, 20th, 25th and 30th days. All the experimental animals were fed with a basal diet containing fish meal, shrimp meal, wheat flour, groundnut de-oiled cake, soybean meal and wheat bran at 3% of the body weight. The numbers of molts were recorded as 20±0.50, 29±1.16, 51±1.87, and 30±1.60 in control, 100, 500 and 2000 IU/kg body weight physiological doses, respectively. Maximum growth was recorded in prawns given 500 IU/ kg dose. Survival was between 58.33 ± 9.13 and 77.77 ± 8.61%. The ash content and calcium level increased significantly (p<0.05) and recorded the highest values in 500 IU/kg physiological dose. However, the inorganic phosphate (P sub(i)) content recorded the highest values in tissue in 2000 IU/kg dose (p<0.05, F = 50.60613). There is no significant difference in calcium contents (p>0.05) in both tissue and molt at 500 and 2000 IU/kg doses. It was found that a higher physiological dose (2000 IU/kg) of vitamin D sub(3) increased the rate of mortality. Results have shown that vitamin D sub(3) has a positive impact on the growth and survival of M. rosenbergii and it interferes with the metabolism of Ca and P sub(i), in tissue, and alters molting frequency. Results on physiological dose suggest an alternative and effective dietary supplementation method of vitamin D sub(3) in the grow-out phase of M. rosenbergii.

Studies on the preparation of fish silage. 3. Dried silage products

An investigation was undertaken on the production of dried products from silver belly (Leiognathus splendens) silage mixed with plant filter materials. Silages produced using hydrochloric acid and/or formic acid when mixed with rice bran or maize meal and dried, yielded powders having an acceptable appearance and a pleasant odour, which are suitable for use in compounded chicken feeds.

Studies on the preparation of fish silage. 4. Economics of production

Economic aspects of producing liquid and dried silage from silver belly (Leiognathus splendens) in Sri Lanka are considered. A discounted cash flow analysis for the production of a dried fish silage/rice bran product suitable for use in compounded poultry feeds shows that the internal rate of return for a 10-year project would be between 34-77% and for a 5-year project between 26-73%. Thus it is concluded that the project would be extremely profitable.

Optimisation of stocking density of Thai silver barb (Barbodes gonionotus Bleeker) in the duckweed-fed four species polyculture system

The optimisation of stocking density of Thai silver barb (Barbodes gonionotus) in the polyculture with Labeo rohita, Catla cat/a and Cyprinus cmpio was investigated in seasonal ponds. Three different stocking densities of Thai silver barb i.e., 5,000, 6,000 and 7,000 fingerlings ha-1 were tested with stocking density of carps fixed at the rate of 10,000 fingerlings ha-1 Duckweed was applied to all ponds supplemented with rice bran and oil cake. There were no significant variations on either water quality parameters or abundance of planktonic organisms due to the different stocking densities of silver barb. A significantly higher fish production (p

Effects of organic manuring (chicken droppings) on growth of Labeo rohita Ham. spawn

An experiment was conducted in six nursery ponds to determine the effect of different doses of organic fertilizer (chicken droppings) on growth and survival of Labeo rohita spawn. Ponds were stocked with four-days old L. rohita spawn at a density of 25 g/decimal and reared for 25 days. Three doses of organic fertilizer viz. 20kg, 10 kg and 5kg per decimal were tried in treatments Tp T2 and T3 respectively with two replication each. Fry were fed twice a day with a mixture of fine mustard oil cake and rice bran at the ratio of 1:1. The highest growth (6.33 em and 3.33 g) and survival rate (72.30%) were observed for the treatment T2. Physico-chemical and biological parameters were found within the productive range for all the treatments.

Effect of farm made feeds on polyculture of shrimp (Penaeus monodon) and three brackishwater finfish species

A study was conducted to evaluate the effects of feed made from locally available ingredients on polyculture of shrimp and three brackishwater finfish species. Hatchery produced post-larvae (PL) of shrimp Penaeus monodon (0.005g) were stocked at the rate of 15,000 PLs/ha. Brackishwater finfish species Liza parsia, Mugil cephalus and Rhinomugil corsula of 0.63-1.4lg collected from local rivers were stocked at the rate 8,000, 1,000 and 2,000/ha, respectively in four treatments. Shrimp and finfishes were fed four different experimental diets composed of fish meal, mustard oil cake, rice bran, oyster shell power and vitamin premixes at the rate of 3-5% estimated crop/day for 195 days. Among four treatments, P. monodon showed comparative better growth in T4 and T3. Finfish L. parsia showed its better performance in treatment T2. Species M. cephalus and R. corsula showed insignificant production. P. monodon showed better growth with diet of fish meal and mustard oil cake@ 28.84 and 33.65%, respectively in T 3 and 19.22 and 43.27%, respectively in treatment T4.

Development of artificial breeding techniques for long-whiskered catfish, Sperata aor and giant river catfish, Sperata seenghala of Bangladesh

Sperata aor and S. seenghala are the two important native catfishes of Bangladesh but commercial farming of these species is not possible due to lack of naturally collected or artificially produced seeds for stocking. Attempts were made to develop techniques for seed production by artificial breeding and nursery-rearing of fries of these catfishes. A total of 60 S. seenghala (750-1,500 g) and 10 S. aor (600-1,000 g) broods were collected from the Brahmaputra river-basin and floodplains in Mymensingh region four months prior to their breeding season. The collected brood fishes were reared in separate earthen ponds with supplementary feeds comprising of rice bran (40%), mustard oil cake (29%), fish meal (30%) and vitamin-premix (1 %). Three experiments were conducted to optimize the hormone dose. A total of nine S. seenghala females weighing from 750 to 1,500 g were given an initial and resolving dose of 12-20 and 16-24 mg PG/kg body weight, respectively. The males weighing from 650-950 g were administered a single dose of 18-26 mg PG/kg body weight at the time of the time of administering the resolving dose to the females. The females ovulated partially and the eggs were examined under a compound microscope, but most of them were found to be less ripe or damaged. Collection of milt by stripping the males was not successful. The testes were taken out and sperm were observed to be non-motile and less developed. In view of stimulating natural propagation of S. seenghala, artificial holes (nests) were constructed in the pond bottom. Each hole was 0.7 m in diameter and 0.3 m in depth. A total of 10 holes were made and then 10 pairs of S. seenghala breeders (800-1,200 g) were stocked in the pond. In mid February, 3,000 fry of S. seenghala with a mean length of 4.60 cm and weight of 0.36 g were collected by repeated netting followed by drying of the pond. The fry were then stocked in a nursery pond and fed with commercial feed (SABINCO starter-1). The average length and weight of the fingerlings were 9.01 cm and 3.95 g, respectively and the estimated survival was 60% after two months of rearing. S. aor did not respond to natural spawning. Further study is essential to develop techniques for their successful artificial and natural breeding.

Influence of stocking density on the culture potential of freshwater catfish Pangasius pangasius in pond

Influence of stocking density on the production of freshwater catfish Pangasius pangasius with formulated feed in ponds was studied. The fish fry were collected from the Meghna river near Chandpur which is a natural breeding ground of this fish. Three stocking densities chosen during this experiment were 5000, 8000 and 11000 fry/ha with an initial weight of 42.57 ±2.51 g. The formulated feed was prepared in the laboratory which contained 45% fish meal, 30% mustard oil cake, 15% wheat bran and 10% rice bran to supply 40.48% protein in feed. The growth of P. pangasius in terms of net weight gain was 409.49, 266.49 and 236.37 g at the 5000/ha, 8000/ha and 11000/ha stocking densities respectively, which was statistically significant (p<0.05). High food conversion ratio (FCR) was also observed during this research study which ranged between 7.06 to 7.72. A production of 2.6 tons/ha at the stocking density of 11000/ha; 2.13 tons/ha at the stocking density of 8000/ha and 2.04 tons/ha at the stocking density of 5000/ha was obtained, while the growth of individual fish at the end of experiment was in opposite order. The result of this experiment indicates that stocking density has significant influence on the culture potential of freshwater catfish P.pangasius.

Culture potentials of mola (Amblypharyngodon mola ), chela (Chela cachius) and punti (Puntius sophore) under monoculture system

To assess the production potentials of small indigenous fish species (SIS) in semi-intensive monoculture system, an experiment was carried out with Amblypharyngadan mala, Chela cachius and Puntius sophore. Three treatments each with three replications were tested with mola, chela and punti individually and the stocking density of each species was 100,000/ha. Organic fertilizer was applied to the ponds at the rate of 1,000 kg/ha at fortnightly and rice bran was supplemented daily at 3% of the total fish biomass. Partial harvesting was made after three months of stocking. Gross production of 805±52.94, 1,120±41.62 and 509±48.81 kg/ha, respectively for mola, chela and punti over a period of six months were obtained. The yield of punti was found to vary significantly (P < 0.05) from that of mola and chela.

Development of low cost feed using local ingredients for culture of freshwater prawn, Macrobrachium rosenbergii in ponds

Two experiments were conducted to formulate and evaluate test diets using locally available ingredients to find out suitable diets for mono and polyculture of Macrobrachium rosenbergii in ponds. The first experiment was conducted from 1 July 2003 to 29 September 2003 in 12 experimental ponds each measuring 30 square meters behind the Fisheries Faculty Building, BAU campus, Mymensingh. Three experimental diets containing 30% protein were formulated using fish meal, meat and bone meal, mustard oilcake, sesame meal and rice bran and assigned to treatments 1, 2 and 3, respectively. A special shrimp feed (Starter-II) from Saudi-Bangla Fish Feed Ltd. was assigned to treatment 4 (Control). Each treatment had three replications. Juveniles of M. rosenbergii (2.90±0.81g) were stocked at the rate of 4/square meter. Prawns were fed three times daily at the rate of 15% of their body weight at the beginning, which was gradually reduced to 10% and 5% for the last two months. There was no significant (P>0.05) difference between the weight gains of prawns fed diets 1 and 4 (control), but they were significantly (P<0.05) higher than those of diets 2 and 3. The FCR values of diets ranged between 2.61 to 3.36 with diets 1 and 4 showing significantly (P<0.05) lower FCR values. The survival rate of prawns ranged between 68 to 78% with prawns fed diets 1 and 4 showing significantly higher survival rate. The production of prawn ranged from 921 to 1,428 kg/ha/90 days and diet 1 gave the highest production. Treatment 1 gave the highest net profit ofTk. 161,980/ha/90 days. The second experiment was conducted from December, 2003 to April, 2004 to see the growth of over wintered M. rosenbergii juveniles in polyculture with indigenous major carps (catla and rohu) in ponds using formulated diets. Three isoenergetic experimental diets formulated using fish meal, meat and bone meal, mustard oil cake, rice bran, wheat bran and molasses, and a shrimp feed 'Golda special feed' from Saudi-Bangla Fish Feed Ltd. were assigned to treatments 1, 2, 3 and 4 (control), respectively, each treatment had two replications. Eight experimental ponds each measuring 80 square meters in the Field Laboratory Complex of the Faculty of Fisheries, BAU campus, Mymensingh were used. The mean initial weights of M. rosenbergii, catla and rohu were 1.60±0.01, 30.0±0.09 and 25.0±0.08 g, respectively. A total of 160 fish and prawn (20,000/ha) were stocked in each pond at the ratio of 2:1:1 (prawn: catla: rohu). Fish were fed at the rate of 3-5% of their body weight. Prawns fed diet 1, 2 and 4 showed higher weight gains compared to diet 3. The weight gain of catla was significantly higher in T1 while in case of rohu was higher in T1 and T4, respectively. There were no significant differences (P>0.05) in the survival rate of fish as obtained from different treatments. The overall total fish production ranged from 2196 to 2679 kg/ha/5 months. The highest production and the highest profit (Tk. 56,531/ha/5 months) was obtained from T1 and the lowest (Tk. 24,932/ha/5 months) from T4.