Successful inoculation of Artemia and production of cysts in the coastal saltpans of Bangladesh II

Bangladesh has no naturally occurring Artemia, and all the growing shrimp hatcheries of the country depend entirely on import of cysts from foreign countries. Following successful inoculation of Artemia and production of cysts for the first time in this country in a coastal saltpan (at Chanua, Banskhali) by the senior author (in 1989-90), a similar second attempt was made under this programme in a saltpan (1000 m super(2)) of Demoshia, Chakaria, Cox's Bazar, Bangladesh between January and April 1992. A total of 1639.9 g (dry weight) of cysts (i.e. 5.46 kg DW/ha/month) have been produced using the Red Jungle Brand, whereas the previous attempt obtained 517 g of cysts (i.e. 2.07 kg DW/ha/month) using the Great Salt Lake Brand.

Standardisation and mass production of shrimp trawlers and other fishing boats in India

This paper deals with the existing fishing crafts, new vessel types, boat building programmes, their standardisation and mass-production in India.

Induction of mitotic and meiotic gynogenesis and production of genetic clones in rohu, Labeo rohita Ham.

Studies were undertaken to produce genetic clones derived from all homozygous mitotic gynogenetic individuals in rohu, Labeo rohita Ham. ln view of this, attempts were made to interfere with the normal functioning of the spindle apparatus during the first mitotic cell division of developing eggs using heat shocks, there by leading to the induction of mitotic gynogenetic diploids in the F1 generation. Afterwards, viable mitotic gynogenetic alevins were reared and a selected mature female fish was used to obtain ovulated eggs which were fertilized later with UV-irradiated milt. Milt was diluted with Cortland’s solution and the sperm concentration was maintained at 10⁸/ml. The UV-irradiation was carried out for 2 minutes at the intensity of 200 to 250 µW/cm² at 28± 1°C. The optimal heat shock of 40°C for 2 minutes applied at 25 to 30 minutes a.f. was used to induce mitotic gynogenesis in first (F1) generation and at 3 to 5 minutes a.f. to induce meiotic gynogenesis in the second (F2) generation. The results obtained are presented and the light they shed on the timing of the mitotic and meiotic cell division in this species is discussed.

Effect of mola (Amblypharyngodon mola Ham.) on the growth and production of carps in polyculture

The effect of introduction of mola (Amblypharyngodon mala) in polyculture with rohu (Labeo rohita), catla (Catla catla) and mirror carp (Cyprinus carpio var. specularis) was studied in semi-intensive culture system in the pond complex of the Fisheries Faculty, Bangladesh Agricultural University, Mymensingh. Mala, a small indigenous fish was found to exert a negative impact on growth and production of carps. After four months' rearing, significant difference (P<0.05) was observed in the net production between the treatments.

Effects of periphyton grown on bamboo substrates on growth and production of Indian major carp, rohu (Label rohita Ham.)

The effects of periphyton, grown on bamboo substrates, on growth and production of Indian major carp, rohu, Labeo rohita (Hamilton), were studied in 10 ponds during July to October '95 at the Bangladesh Agricultural University, Mymensingh. Five ponds were provided with bamboo substrates (treatment I) and the rests without bamboo substrates (treatment II). It was revealed that there had been no discernible difference in the water quality parameters between treatments. A large number of plankton (30 genera) showed periphytic nature and colonized on the bamboo substrates. The growth and production of fish was significantly (p<0.05) higher in the ponds with bamboo substrates as compared to the ponds without substrates. The net production of rohu in treatment I was about 1.7 times higher than that of treatment II. Fish production was as much as 1899 kg/ha over a culture period of 4 months in the periphyton-based production system.

Growth and production of Nile tilapia (Oreochromis niloticus Lin.) in irrigated Boro rice under floodplain environment

The experiment was conducted at BRRI Regional Station, Habiganj during 1994-95 to evaluate the growth and economic performance of Nile tilapia, Oreochromis niloticus, fish reared in the field of irrigated boro rice with different fertilizer levels. Grain yield of rice was not affected by fish culture. It was observed that 50% of recommended fertilizer was enough to produce increased rice yield (8-10 t/ha) at floodplain environment and additional yield was obtained with the increasing fertilizer rates. Results further indicated that O. niloticus could successfully be reared in the field of irrigated boro rice with recommended fertilizer level. Larger size of fingerlings at release had improved recovery percent, body weight gain and higher fish yield. Results also revealed that rice + fish production system produced higher net return than the system with rice alone.

Effect of different feeds on growth, survival and production of African catfish (Clarias gariepinus Burchell)

An experiment was conducted to study the effect of different feeds on growth survival and production of African catfish (C. gariepinus) in six cemented tanks (3m² each) over a period of 120 days. Three different feeds namely Feed A (Saudi-Bangla fish feed, 33.43% protein), Feed B (formulated feed, 40.12% protein) and Feed C (chicken raw intestine, 59.58% protein) were applied to treatments I, II and III respectively. Each of the tanks was stocked with 24 fry with mean initial body weight of 2.56±0.06 g. Feeds were supplied to the fish ad-libitum daily in two instalments. Significantly highest weight gain was obtained in treatment III, however, survival rate was low compared to other treatments. The feed conversion ratio (FCR) values ranged from 2.52-6.4. Survival rate of fish varied between 83 and 96%. Treatment II yielded the highest (5000 kg/ha/120 days) production with the highest survival rate of fish. On the basis of survival rate and production, it is suggested that the formulated feed (Feed B) is suitable for the culture of C. gariepinus in cemented tanks.

Effect of Lates calcarifer seed stocking on the survival and production of Indian major carps reared in a freshwater coastal pond in Konkan region

The experiment indicated feasibility of culturing Indian major carps seed with Lates calcarifer, if the size of Indian major carps is larger. In the present investigation the average growths of Catla catla, Labeo rohita and Cirrhinus mrigala are recorded as 1193, 1120 and 821 g, respectively, during the year 1997-98, 1998-99 and 1999-2000 in 7.5 months. Complete harvesting of L. calcarifer is essential before stocking of new seed. The average growth of L. calcarifer was recorded as about 670 g during the above period. About 5000 numbers of Indian major carps fry per hectare can be stocked under the present culture system

Effect of different feeds and water salinities on the cyst production of brine shrimp, Artemia sp.

Effect of different feeds and salinities on cyst production in Artemia sp. was studied. Among the 12 different feeds used, ragi powder suspension showed the best growth and cyst production. A wide range of salinity from 90 to 210‰ was used to study its effect on cyst production. Among all these treatments, 130‰ salinity was found suitable for induction of oviparity and cyst production in the San Francisco Bay strain of Artemia sp.

Cryopreservation of sperm of common carp, Cyprinus carpio and silver barb, Barbonymus gonionotus for genetically improved seed production

Experiments were conducted to develop and standardize the protocols for cryopreservation of sperm of common carp, Cyprinus carpio and also for using the cryopreserved sperm for fertilization of eggs. Nine extender solutions as Alsever's solution, kurokura-1, kurokura-2, urea egg-yolk, egg-yolk citrate, 0.6% glucose, 0.9% NaCl, Ma and Mb, and five cryoprotectants namely ethanol, methanol, dimethylsulfoxide (DMSO), dimethylamine (DMA) and glycerol were tested. The cryoprotectants were mixed at 10% concentration of the extenders (v/v) to make the cryodiluents. Milt and cryodiluents were mixed at a ratio of 1:9 for Alsever's solution, kurokura-1, kurokura-2, 0.6% glucose and 0.9% NaCl, 1:4 for urea egg-yolk, egg-yolk citrate, Ma and Mb. Among the cryodiluents Alsever's solution mixed with either ethanol or methanol was found to be suitable and it produced more than 90% and 80% spermatozoan motility at equilibrium and post-thaw periods, respectively. Kurokura-1 and kurokura-2 when mixed with the same cryoprotectants showed good spermatozoan motility at equilibrium period (80-90%) but the motility was reduced (30-55%) at post-thaw state. Other extenders did not produce acceptable sperm-motility and in some cases the frozen milt became clotted. Different dilution ratios (1:1, 1:2, 1:4, 1:5, 1:7, 1:9, 1:12, 1:15, 1:20) were formulated for obtaining a suitable milt dilution, the dilution ratio of 1: 9 (milt : cryodiluent) demonstrated the highest post-thaw spermatozoan motility (80%) in Alserver's solution. The optimum concentration of cryoprotectants in the cryodiluents was determined, 10% concentration level was found to be effective to produce the highest number of spermatozoan motility in comparison to the other concentrations (5%, 15%, 20% 30%). Sperm preserved with the cryodiluent Alsever's solution along with either methanol or ethanol was found to be effective to fertilize eggs and produce hatchlings. The hatching rates ranged between 1.48% and 14.76%, compare to control. The fish produced through use of cryopreserved sperm and normal sperm were found to grow well and no significant (P<0.05) growth difference was observed between them. In case of silver barb, Barbonymus gonionotus, sperm tested against six extenders such as egg-yolk citrate, urea-egg-yolk, kurokura-1, kurokura-2, 0.9% NaCl and modified fish ringer (MFR) solution. Cryoprotectants used were the same as those of C. carpio. Milt was diluted with the cryodiluent at a ratio of 1:4 for egg-yolk citrate and urea-egg-yolk, 1:5 for kurokura-1 and 1:9 for 0.9% NaCl, MFR and kurokura-2. The cryoprotectant concentration was maintained at 10% of the extender (v/v) in all the cases. Among the extenders, egg-yolk citrate and urea-egg-yolk mixed with 10% DMSO, methanol and ethanol produced 50% post-thaw spermatozoan motility, whereas DMA and glycerol provided only 10% motility. Trials on milt dilution ratio and cryoprotectant concentration are being conducted. Fertilization trials are also underway.