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The rotifer Brachionus plicatilis plays an important role in prawn hatcheries. It tolerates a wide range of salinities. The present experiments were conducted to find the optimum salinity for its mass culture. Experiments conducted on various ranges of salinities starting from 0 to 40 ppt at an interval of 5 ppt revealed that Brachionus plicatilis did not survive at salinities 0 and 40 ppt. Optimum salinity studies conducted at 5-15 ppt with an interval of 1 ppt showed that the production of 70 individuals/ml was highest at 10 ppt salinity and the doubling time ranged from 1.728 to 1.317 days.