Cultural methods of detection for microorganisms: recent advances and successes

Most microbiological methods require culture to allow organisms to recover or to selectively increase, and target organisms are identified by growth on specific agar media. Many cultural methods take several days to complete and even then the results require confirmation. Alternative techniques include the use of chromogenic and fluorogenic substances to identify bacteria as they are growing, selective capture using antibodies after short periods of growth, molecular techniques, and direct staining with or without flow cytometry for enumeration and identification. Future microbiologists may not use culture but depend on the use of specific probes and sophisticated detection systems.

History of the Bay Scallop, Argopecten irradians, Fisheries and Habitats in eastern North America, Massachusetts through northeastern Mexico

This is a broad historical overview of the bay scallop, Argopecten irradians, fishery on the East and Gulf Coasts of North America (Fig. 1). For a little over a century, from about the mid 1870’s to the mid 1980’s, bay scallops supported large commercial fisheries mainly in the U.S. states of Massachusetts, New York, and North Carolina and on smaller scales in the states in between and in western Florida. In these states, the annual harvests and dollar value of bay scallops were far smaller than those of the other important commercial mollusks, the eastern oysters, Crassostrea virginica, and northern quahogs, Mercenaria mercenaria, but they were higher than those of softshell clams, Mya arenaria (Table 1). The fishery had considerable economic importance in the states’ coastal towns, because bay scallops are a high-value product and the fishery was active during the winter months when the economies in most towns were otherwise slow. The scallops also had cultural importance as a special food, an ornament owing to its pretty shell design, and an interesting biological component of

Economics and Hawaii's Marine Fisheries

This paper reviews economic research conducted on Hawaii's marine fisheries over the past ten years. The fisheries development and fisheries management context for this research is also considered. The paper finds that new approaches are required for marine fisheries research in Hawaii: A wider scope to include other marine resource and coastal zone issues, and increased and closer collaboration between researchers and the fishing community.

An ecological characterization of the Stellwagen Bank National Marine Sanctuary Region: oceanographic, biogeographic, and contaminants assessment

The mission of NOAA’s National Marine Sanctuary Program (NMSP) is to serve as the trustee for a system of marine protected areas, to conserve, protect, and enhance their biodiversity, ecological integrity, and cultural legacy while facilitating compatible uses. Since 1972, thirteen National Marine Sanctuaries, representing a wide variety of ocean environments, have been established, each with management goals tuned to their unique diversity. Extending from Cape Ann to Cape Cod across the mouth of Massachusetts Bay, Stellwagen Bank National Marine Sanctuary (NMS) encompasses 2,181 square kilometers of highly productive, diverse, and culturally unique Federal waters. As a result of its varied seafloor topography, oceanographic conditions, and high primary productivity, Stellwagen Bank NMS is utilized by diverse assemblages of seabirds, marine mammals, invertebrates, and fish species, as well as containing a number of maritime heritage resources. Furthermore, it is a region of cultural significance, highlighted by the recent discovery of several historic shipwrecks. Officially designated in 1992, Stellwagen Bank became the Nation’s twelfth National Marine Sanctuary in order to protect these and other unique biological, geological, oceanographic, and cultural features of the region. The Stellwagen Bank NMS is in the midst of its first management plan review since designation. The management plan review process, required by law, is designed to evaluate, enhance, and guide the development of future research efforts, education and outreach, and the management approaches used by Sanctuaries. Given the ecological and physical complexity of Stellwagen Bank NMS, burgeoning anthropogenic impacts to the region, and competing human and biological uses, the review process was challenged to assimilate and analyze the wealth of existing scientific knowledge in a framework which could enhance management decision-making. Unquestionably, the Gulf of Maine, Massachusetts Bay, and Stellwagen Bank-proper are extremely well studied systems, and in many regards, the scientific information available greatly exceeds that which is available for other Sanctuaries. However, the propensity of scientific information reinforces the need to utilize a comprehensive analytical approach to synthesize and explore linkages between disparate information on physical, biological, and chemical processes, while identifying topics needing further study. Given this requirement, a partnership was established between NOAA’s National Marine Sanctuary Program (NMSP) and the National Centers for Coastal Ocean Science (NCCOS) so as to leverage existing NOAA technical expertise to assist the Sanctuary in developing additional ecological assessment products which would benefit the management plan review process.

Bibliography of synthesis documents on selected coastal ocean topics

This compilation of references to works which synthesize information on coastal topics is intended to be useful to resource managers in decision making processes. However, the utility must be understand in terms of its limited coverage. The bibliography is not inclusive of all the published materials on the topics selected. Coverage is clearly defined in the following paragraph. The time span of the bibliography is limited to references that were published from I983 to 1993, except for a last-minute addition of a few 1994 publications. All searches were done in mid- to late-1993. The bibliography was compiled from searches done on the following DIALOG electronic databases: Aquatic Sciences and Fisheries Abstracts, BlOSlS Previews, Dissertation Abstracts Online, Life Sciences Collection, NTlS (National Technical lnformation Service), Oceanic Abstracts, Pollution Abstracts, SciSearch, and Water Resources Abstracts. In addition, two NOAA electronic datases were searched: the NOAA Library and lnformation Catalog and the NOAA Sea Grant Depository Database. Synthesis of information is not an ubiquitous term used in database development. In order to locate syntheses of required coastal topics, 89 search terms were used in combinations which required 10 searches from each file. From the nearly 6,000 citations which resulted from the electronic searches, the most appropriate were selected to produce this bibliography. The document was edited and indexed using Wordperfect software. When available, an abstract has been included. Every abstract was edited. The bibliography is subdivided into four main topics or sections: ecosystems, coastal water body conditions, natural disasters, and resource management. In the ecosystems section, emphasis is placed on organisms in their environment on the major coastlines of the U.S. In the second section, coastal water body conditions, the environment itself is emphasized. References were found for the Alaskan coast, but none were found for Hawaii. The third section, on natural disasters, emphasizes environmental impacts resulting from natural phenomena. Guidelines, planning and management reports, modelling documents, strategic and restoration plans, and environmental economics related to sustainability are included in the fourth section, resource management. Author, geographic, and subject indices indices are provided. The authors would like to thank Victor Omelczenko and Terry Seldon of the NOAA Sea Grant Office for access to and training on the NOAA Sea Grant Depository Database. We are grateful also to Dorothy Anderson, Philip Keavey, and Elizabeth Petersen who reviewed the draft document.

Sediment quality triad assessment in Kachemak Bay: characterization of soft bottom benthic habitats and contaminant bioeffects assessment condensed. A NOAA/NOS/NCCOS/CCMA Special Report

A baseline environmental characterization of the inner Kachemak Bay, Alaska was conducted using standardized National Status and Trends Bioeffects Program methods. Three sites near the village of Port Graham were also sampled for comparison. Concentrations of over 120 organic and metallic contaminants were analyzed. Ambient toxicity was assessed using two bioassays. A detailed benthic community condition assessment was performed. Habitat parameters (e.g. depth, salinity, temperature, dissolved oxygen, sediment grain size, and organic carbon content) that influence species and contaminant distribution were also measured at each sampling site. The following is the synopsis of findings • Sediments were mostly mixed silt and sand with pockets of muddy zones. Organic compounds (PAHs, DDTs, PCBs, chlorinated pesticides) were detected throughout the bay but at relatively low concentrations. With some exceptions, metals concentrations were relatively low and probably reflect the input of glacial runoff. • Homer Harbor had elevated concentrations of metallic and organic contaminants. Concentrations of organic contaminants measured were five to ten times higher in the harbor sites than in the open bay sites. Tributyltin was elevated in Homer Harbor relative to the other areas. • There was no evidence of residual PAHs attributable to oil spills, outside of local input in the confines of the harbor. • The benthic community is very diverse. Specific community assemblages were distributed based on depth and water clarity. Species richness and diversity was lower in the eastern end of the bay in the vicinity of the Fox River input. Abundance was also generally lower in the eastern portion of the study area, and in the intertidal areas near Homer. The eastern portions of the bay are stressed by the sediment load from glacial meltwater. • Significant toxicity was virtually absent. • The benthic fauna at Port Graham contained a significant number of species not found in Kachemak Bay. • Selected metal concentrations were elevated at Port Graham relative to Kachemak Bay, probably due to local geology. Organic contaminants were elevated at a site south of the village.

Biodiversity in floodplains with special reference to artificial stocking

A five years investigation on fish biodiversity in connection with artificial stocking was conducted in three south-western floodplains of Bangladesh from 1992 to 1996. The ten top most available and ten rarest fish species were identified. Puntius sp., Channa punctatus, Mystus sp., Anabus testudinius, Ambasis sp., Colisha sp. and Macrobrachium sp. etc. were the most common available species. On the other hand, Mystus aor, Notopterus chitala, Clupisoma garua, Aplocheilus panchax, Ctenophmyngodon idella etc. were the rarest species. However, the most abundant and the rarest fish species behaved differently in different floodplains in different years. Shannon diversity index was used to assess the extent of diversity in different years. The study revealed that the artificial stocking programme, to some extent, influenced the biodiversity in floodplains.

Standardization of polyphosphate treatment for tiny prawns (Parapenaeopsis stylifera) with special reference to its rehydration capacity and organoleptic quality

In the present study, an attempt was made to explore the benefits of polyphosphate for enhancement of dried prawn (Parapenaeopsis stylifera) quality commercially known as "sode" in Maharashtra coast. Dip treatment in polyphosphate solution at different concentrations (viz., 3, 5, 7 and 10%) was given to pealed and no deveined P. stylifera for different durations (viz., 1, 2, 3, 4 and 5 min). Treated prawns were dried and subjected to rehydration capacity test and organoleptic evaluation. Among the different treatments, rehydration capacity was found to increase with the increased duration and concentration of treatment. Tiny prawns treated with sodium tripolyphosphate solution at the rate of 5% concentration for 5 minutes showed an increase in pH from acidic to alkaline, and had better quality with respect to rehydration capacity and textural attributes as compared to other concentrations and durations of polyphosphate treatment.

The role of social,economic and cultural factors in the management of satellite lake fisheries in Uganda

The fisheries of small lakes are important for producing fish for local populations not clear the larger lakes.The satelite support important fisheries and other economic activities like fishing water for domestic purpose and tourism besides socio-cultural functions.

A record of Probopyrus bithynis (Richardson, 1904) in Macrobrachium rosenbergii (De Man) from coastal Andhra Pradesh, India, with special reference to host-parasite relationship

An incidence of bopyrid isopod infestation was observed in giant freshwater prawn, Macrobrachium rosenbergii (de Man) juveniles (40-60 mm/0.9-1.5 g) in a scampi culture farm in East Godavari district of Andhra Pradesh. The presence of parasite was observed by conspicuous boil like swelling of the branchial chamber where the parasite was found lodged on the gills. The infested gill was highly compressed and necrosed. Only one branchial chamber was infested by the parasite while the other gill was normal. The infested prawns were thin and emaciated and showed retarded growth. The parasite was identified as Probopyrus bithynis (Richardson, 1904) which caused inhibition of ventilation due to its permanent lodging in the branchial chamber and impaired the gaseous exchange by gills. It was also observed that this parasite caused parasitic castration in the infested prawns.

Input-output relationship and economics of pangas monoculture and carp-pangas polyculture in two districts of Bangladesh

An attempt was made to study the input-output relationships and economics of pangas monoculture and carp-pangas polyculture in Bangladesh. By analyzing the data collected from 50 pangas farms and 55 carp-pangas farms, the study has investigated the production systems of two technologies and the effects of fingerling stocking and applications of feed and fertilizer on fisheries income. The data were collected from the fishermen of Trishal and Bhaluka of Mymensingh district, and Kahaloo and Adamdighee of Bogra district during 2001-02. For pangas monoculture, the stocking density was 31,561 per ha while it was 55,017 per ha in carp-pangas polyculture. Most of the farmers used urea, TSP and lime before stocking. Rice and wheat bran happened to be the most common feed ingredients for both types of culture in general. Other important ingredients used were mustard oil-cakes, rice polish, wheat flour, fish meal, bone meal, soybean meal and poultry litter. In terms of quantities, rice bran and wheat bran dominated the farmers list. Rice and wheat bran together constituted about 60% of all studied feeds. Feed cost constituted 59.13% of total costs for pangas monoculture and 67.44% for carp-pangas polyculture. Per ha productions of pangas and carp-pangas in a single culture cycle were 15,508 kg and 19,745 kg, respectively. Per ha gross profits were estimated to be Tk 310,311 and Tk 464,418 for pangas monoculture and carp-pangas polyculture, respectively. Net profit appeared to be Tk 264,216 per ha for pangas monoculture and Tk 416,509 per ha for carp-pangas polyculture. The BCRs calculated were 1.46 and 1.68 for monoculture and polyculture, respectively. The break-even costs per kg of fish were estimated at Tk 36.93 for pangas and Tk 30.93 for mixed species which was much lower than the prices the producers received. Break-even productions were estimated at 10,702 kg per ha for pangas monoculture and 11,784 kg per ha for carp-pangas polyculture. Fingerling and feed cost, and pond size significantly explained the variation of income from pangas monoculture. These factors have significantly influenced the income from the crop. Functional analysis shows that 1% increase in the feed cost might increase 0.51% of pangas income and 0.41% in carp-pangas income. No other inputs had shown this much of responses to increasing income from a fish.

Year-round and rotational freshwater prawn, Macrobrachium rosenbergii and paddy farming: soil quality, production and economics

A study was carried out at five upazillas (Dumuria, Fakirhat, Pirojpur sadar, Gopalgonj sadar and Kalia) of five southern districts of Khulna, Bagerhat, Pirojpur, Gopalgonj and Narail to understand the comparative production performance and any effect on soil quality in case of year-round golda (Macrobrachium rosenbergii), alternate prawn-paddy and year-round paddy farming. A direct interview of 55 farmers was taken, using pretested questionnaire, and soil samples were taken from selected farms before and after each crop. Among the five upazillas, farmers in Gopalgonj sadar and Kalia are not practicing year-round golda. Rotational golda-paddy farming has been recorded to result in maximum profit, with the highest of Tk. 310,912/ha/year in 2003 at Dumuria. Only paddy farming is less profitable than other two cropping patterns, irrespective of study sites. The nutrient status of soil in rotational golda and paddy farming has been found improved, compared to that of only golda or paddy farming.

Effects of different types of feeds on growth and production of tiger shrimp, Penaeus monodon at Bagerhat region, Bangladesh

An experiment was carried out in farmers' gher (shrimp farm) at Bagerhat sadar upazilla, Bagerhat to ascertain the effects of three different types of feeds on the production and economics of brackishwater shrimp, Penaeus monodon for a period of 120 days. There were three treatments such as T1 (BFRI dough feed containing of 30% fish meal, 10% protein conc., 10% soya meal, 15% mustard oil cake, 18% rice bran, 5% maize, 10% wheat flour, 1% oyster shell powder and 1% vitamin premix), T2 (Commercial diet Saudi-Bangla grower) and T3 (Saudi-Bangla special feed). Each treatment had two replicates and the stocking of shrimp in each gher was 3 nos/m². Water quality parameters did not differ significantly among the treatments except water depth. Average production and net return of shrimp in different treatments varied from 404.0 to 509.0 kg/ha and Tk. 56,493.99-Tk. 84,209.60, respectively. T2 showed significantly (p<0.05) the highest production and economic return. The result of the study implied that T2 is more suitable and economically viable than that of other treatments for shrimp farming.

The socio-economic and cultural structures of the fisherfolk communities

Fishing communities that have exploited the resource for generations constitute the main stakeholder groups in the fisheries of Lake Victoria. Several studies have examined Uganda's Lake Victoria fishing communities and characterised key stakeholders at community level over the last decade (SEDAWOG 1999a and b; Geheb 1997; FeSEP 1997; Kitakule 1991). The communities are made up of scattered settlements at the shores and on islands. The categories of people living in these communities include fishers who consist primarily of large numbers of male youths who provide labour to boat and gear owners. There are resident and non-resident fish traders who after securing their supplies at the beaches, depart for their market destinations. In addition, there are fish processors, mostly operating traditional and improved smoking kilns. Many other people, dealing in provisions and supplies also stay at the beaches, their activities depending on the level of fish catch. The fishing communities of Lake Victoria, Uganda, include auxiliary livelihood activities such as boat building, net repairing and transportation; bait supply and beachside kiosks, video halls and retail shop business. Other economic activities are brick making, charcoal burning/wood trade, farming and livestock keeping.

Socio-economics of fishing communities on Uganda water bodies

Control and management of Uganda fishery resources has been hindered by among other factors the multispecies nature of the resource and the characteristic behaviour of the fishing communities. Fishermen have both genuine and uncompromising attitudes as to why they carry out certain fishing technologies.All fishing activities aim at maximizing the catches or profits while others may fish on a small scale for subsistence. Sensitizing the fisherfolk on the appropriate fishing technologies, importance of a well regulated fishery exploitation and their participation in control and management of the resource would enhance or lead to increased and sustainable fish production. Socio-economics of fishing technologies were therefore examined using prepared questionnaires and reasons why the fishing communities behave the way they do established