Changes of enzymatic activity during larval development of carp, Catla catla (Ham.)

Quantitative assays of trypsin, amylase and alkaline phosphatases were made in relation to age and food during the larval development of the Indian major carp Catla catla. The responses of all the test enzymes to age and food were identical. No enzymes were detected from the fertilized eggs. Detectable amount of enzymes were first observed in the first day old hatchlings. All the test enzymes in the group fed normal feed tended to rise gradually with advancement of age till day 22 after which an asymptotic level was attained. Absence of food throughout the rearing period caused the enzymatic activity of the larva to remain at the lowest level throughout. When starvation was followed by feeding, enzymatic activity in the former group was consistently higher than that of latter, suggesting that feeding activity was primarily responsible in maintaining the enzymatic activity of carp larva. The enzymatic activity of zooplankton was significantly higher than carp larva till day 6 to 12 after which the latter exceeded the former implying that carp larva during development utilizes the exogenous enzymes of zooplankton.

Post-larval production of Macrobrachium rosenbergii using mass-cultured live food organisms

Macrobrachium rosenbergii post-larvae were produced in 1992 and 1993 using Artemia nauplii and cultured zooplankton Brachionus plicatilis (rotifier), Apocyclops dengizicus (copepod) and Moina sp. (cladoceran) supplemented with chopped Tubifex worms. In 1992 (first trial) two experiments were carried out under water temperature range of 24.5 to 28°C and 26.0 to 28.5 °C respectively and corresponding post-larval production was 5.6% and 86.3%. The duration of experiments was 58 and 40 days. During second trial in 1993 water temperature varied between 25.0 to 27.0°C. At the end of 59 days the post-larvae were found to be 44% of the total number of larvae stocked on the first day.

Substitution of Artemia with Brachionus in different ratios during larval rearing of Macrobrachium rosenbergii and evaluation of their feed efficacy

Larval growth during stage I-VIII was studied in Macrobrachium rosenbergii. Duration in moult periodicity were recorded-during larval development period, larvae were fed with Brachionus (grown on Baker's yeast and also Brachinous raised through organic manuring in outdoor culture containers). The performance of the feed was evaluated through substitution of Brachionus in the feeding protocol, in lieu of Artemia 1st instar. The Artemia, Brachionus substitution ratio of 75:25 was found to be most efficient. The study also indicates that the comparative growth rate of Brachionus plicatilis is higher in manure loaded tanks than with Baker's yeast. Growth rate "Y'' in culture tank being 0.245 and 0.112 and corresponding duplicating time (Td) too was found to be 2.855 and 6.365 respectively in tanks manured/enriched with pig manure.

Effect of Lates calcarifer seed stocking on the survival and production of Indian major carps reared in a freshwater coastal pond in Konkan region

The experiment indicated feasibility of culturing Indian major carps seed with Lates calcarifer, if the size of Indian major carps is larger. In the present investigation the average growths of Catla catla, Labeo rohita and Cirrhinus mrigala are recorded as 1193, 1120 and 821 g, respectively, during the year 1997-98, 1998-99 and 1999-2000 in 7.5 months. Complete harvesting of L. calcarifer is essential before stocking of new seed. The average growth of L. calcarifer was recorded as about 670 g during the above period. About 5000 numbers of Indian major carps fry per hectare can be stocked under the present culture system

Survival of Staphylococci on frozen fish

Using Staphylococcus aureus as the test culture it has been shown that cell injury occurs in two phases during freezing and storage at temperatures below freezing. Certain constituents of fish muscle appear to protect the cells during both phases of injury. The survival of bacteria on fish muscle is not influenced by the rate at which the fish muscle was frozen prior to inoculation. There was no significant difference between growth of bacteria on quick frozen and slow frozen fish muscle after thawing. However there appeared to be a slight tendency for the lag phase of growth to be extended on thawed quick frozen fish muscle when compared with thawed slow frozen muscle.

Use of crude salt in rearing of freshwater giant prawn, Macrobrachium rosenbergii (de Man) larvae

Three different types of culture media: (i) 100% brine (B 100 ), (ii) 75% brine and 25% crude salt (B 75 CS 25 ), and 50% brine and 50% crude salt (B 50 CS 50) were tested to evaluate the possible use of brackish water reconstituted from the crude salt for the production of M. rosenbergii post-larvae. The production rate of 25.26±0.20 PI/l with a corresponding survival rate of 84.20±0.66% was significantly higher (P<0.05) for the larvae reared on B100 than that of 22.10±0.57 Pl/l with a corresponding survival rate of 73.68±1.89% on B50CS50. Larvae cultured on B75CS25 did not show any significant difference (P<0.05) in production as well as in survival of post-larvae than that on B100. The result shows that, for rearing of prawn larvae, use of brine can be replaced up to 25% without any undue reduction in production of post-larvae. However, the production as well as survival rate of post-larvae with 50% replacement (B50CS50) is also appreciable. It is assumed that the mineral constituents of natural seawater might have some triggering effects on prawn larvae in closing their larval cycle.

Effect of pond aeration on growth and survival of Penaeus monodon Fab.

The effect of paddle wheel aeration on shrimp growth and survival were studied at a commercial farm at Chandipur coast of Orissa, India, at different stocking densities of Penaeus monodon. Four different aeration patterns were adopted and evaluated. Influence of individual aeration pattern on average survival rate was not highly significant (p<0.05) at different stocking densities, while different aeration patterns had significant influence (psurvival rate of P. monodon. It was also estimated that 1.77 hp (aerator) is needed for every 1000 kg shrimp biomass which corresponds to 1 hp/565 kg biomass of shrimp. Higher growth rate was mostly observed during 63-98 days of culture, when six 2 hp aerators were in use. Size variation in growth was higher during initial stage of rearing, while it was reduced to significant level towards the last phase of rearing as number of aerator and hour of operation increased.

Larval development of Micippa platipes Ruppell, 1930, reared under laboratory conditions (Crustacea, Decapoda, Majidae)

The ovigerous female of Micippa platipes Ruppell, 1830, captured from Buleji (Karachi, Pakistan) on February 7, 1993 and was kept under the laboratory conditions. On February 27, 1993 larvae were hatched in prezoeal stage. The presoeal stage of M. platipes passed through two zoeal stages within three to five days at room temperature (17-20C). The larvae are described, illustrated and compared with the larval account of Micippa thalia (Herbst, 1803) given by Kurata, 1969.

Larval development of a semi terrestrial mangrove sesarmine crab Chasmagnathus convexus (Crustacea: Decapoda: Brachyura: Grapsidae) reared in laboratory

Four zoeal stages and one megalopal stage were identified in laboratory reared semiterrestrial mangrove sesarmine crab Chasmagnathus convexus. At an average salinity and temperature of 20±1% and 19.2±0.2°C, the megalopa was attained 24 days after hatching. Morphologically, the first zoae of C. convexz1s is very similar to those of other species of the genus Chasmagnathus as well as species of the genus Helice, in that view all share the following characteristics: lateral spine on the carapace, three pairs of setae on the posterior margin of the telson furca, one plus five setae on the endopod of the maxillule, and two plus two setae on the endopod of the maxilla. The differences between the first zoea and megalopa of and those of its congeners are discussed.

Pond production of Artemia in Pakistan

Currently our government and the private sectors are very much interested in the establishment of marine aquaculture. For the successful operation in aquaculture of finfishes and shellfishes, the basic requirement is the suitable diet, apart from proper environment. For the larvae, juveniles and adult stages of the culturing organisms the live Artemia is the ideal food. The aquaculturists the worldover are using live food for their culturing organisms, as the live food played an important role in the dietary management of aquaculture of finfishes and shellfishes (Sorgeloos and Kulasekarapandian, 1984), particularly during larval stages. The live nauplii of Artemia are used in aquaculture of finfishes and shellfishes due to being nutritionally balanced, non polluting, economically bearable, viable and readily acceptable to the culturing species. The adult Artemia is also used for feeding the aquarium fishes particularly so when there is a clear abundance of this resource which is cheaper and can economically compete with alternative artificial diet. By the use of Artemia the aquaculturists may obtain optimum growth and survival rate of the organisms. The life cycle of Artemia is very short, which is completed within two weeks especially during dry season in highly saline waters, the two weeks old Artemia starts producing cysts. These cysts become ready to harvest within a week.

Substitution of live feed by formulated diet: effect on the growth and survival of Betta splendens (Regan) fry

The present work evaluates the effectiveness of partial or total replacement of live feed (LF) (Tubifex) together with formulated diet (FD) for Betta splendens. Three hundred Betta splendens fry of uniform size (mean weight 0.19±0.01g) were equally distributed in five treatment groups with three replicates in glass aquaria of 351itre capacity. Fishes were given diets at different ratio of LF and FD viz. T1(C) 100% LF; T2 75% LF, 25% FD; T3 50% LF, 50% FD; T4 25% LF, 75% FD and T5 100% FD and the experiment continued for 105 days. T2 group registered highest (P<0.05) % body weight gain (125.61±0.26) and specific growth rate (2.34±0.02), which was similar to T1 and T3 groups. Lowest FCR was recorded in T2 (2.40±0.11) group, which was similar to Tl, T3 and T4 groups. Highest (P<0.05) PER was observed in T4 (1.00±0.03) group, which was similar to T3 and T5 groups. At the end of experiment, highest % survival was recoded in T1, T2 and T3 groups (96.67±1.67), which was similar to T4 group. From the study, it is concluded that LF can be successfully replaced up to 75% by FD without any adverse effect on the growth and survival of Betta splendens.

Salinity and survival of Martesia striata (Linn) in Cochin harbour

The effects of salinity variations on the survival of Martesia striata from Cochin harbour are presented. It is observed that at least a few of the animals survive the low saline conditions during monsoon. Laboratory experiments showed the lethal salinity as 6‰ when animals acclimatised in 34‰ were subjected to abrupt changes in salinity. But acclimatisation to 17‰ salinity showed a downward shift in the lethal salinity to 4‰. The present observations indicate that M. striata is euryhaline arid the extent of tolerance to lower salinities depends on the degree of acclimatisation.

Observations on the ingression and distribution of larval prawns in the Nethravati-Gurupur estuary, Mangalore

The spatial and temporal distributions of larval prawns of penaeids and non-penaeids in the estuarine waters of Mangalore were studied. Larvae appear to be passively brought in by the incoming flood tides to the estuary, enjoy a wider distribution throughout the estuarine complex with abundance towards the mouth. The distribution of larval prawns was more in Nethravati than in the Gurupur stretch. The influence of temperature, hydrogen-ion-concentration, salinity and dissolved oxygen on the distribution of larvae in the estuaries is discussed. Inference on spawning seasons of commercially important prawns in the neighbouring waters has been arrived at based on their larval abundance.

Experiments on the induced spawning and larval rearing of the milkfish, Chanos chanos (Forsskal) in 1979

This paper summarizes the results of the experiments on the induced breeding and larval rearing of milkfish (Chanos chanos) during the 1979 season. Milkfish larvae could be reared successfully without the use of trochophore larvae of oysters as feed during the first few days. In order to induce the ovulation of wild adult milkfish a higher dose of human chorionic gonadotropin hormone is required.