Postmortem changes in hilsa fish. Pt. 1. Studies on rigor-mortis, typical yields and protein composition of dark and white muscle of hilsa fish (Tenualosa ilisha Ham.)

Hilsa (Hilsa ilisha) caught by gill net were immediately killed by cranial spiking. Three fish were kept in ice (0°C) and three other at room temperature (33°C) to follow development of rigor mortis and changes in muscle pH. The rest were frozen stored at -20°C. Rigor started 15 minutes after death in all fish and reached full rigor (100%) state in 2 and 4 hours respectively in fish kept at 33° and 0°C. The fish at 33°C deteriorated 16 hours after while in full rigor but those at 0°C lasted 26 hours of death without deterioration. Freshly caught hilsa had a muscle pH around 7 which decreased with time rapidly at 33°C and slowly at 0°C. The relative proportion of protein fraction in white and dark muscle of fish stored at 0°C and -20°C were also studied. The proportion of dark muscle was 30.34% of the white muscle. White muscle in fish at 0°C was found to contain 32.0% sarcoplasmic, 57.6% myofibrilla, 9.4% alkali-soluble and 1.1% stroma protein whereas these proteins in dark muscle were 29.9%, 58.4%, 9.8% and 1.9% respectively. The protein fractions of white muscle in frozen-fish were found 27.6% sarcoplasmic, 64.7% myofibrilla, 6.0% alkali-soluble and 1.7% of stroma protein whereas they were 30.6%, 58.6%, 8.9 and 1.9% for dark muscle. Some changes occurred in protein composition during frozen storage. The relative amounts of sarcoplasmic, alkali soluble and stroma protein fractions decreased while myofibrilla fraction increased in frozen condition. This may be attributed to drip loss of soluble protein during thawing.

Study on fishing gears, species selectivity toward gears and catch composition of BSKB beel, Khulna, Bangladesh

An investigation on the types of fishing gear used and their species selectivity and effects on fishes of BSKB beel in Khulna was conducted from June '95 to January '96. Fishermen were found to follow 6 fishing techniques viz., netting, trapping, angling, spearing, dewatering and hand picking. Among them 23 types of the fishing gear was recorded to be used by the fishermen of which 7, 8, 4 and 4 are nets, traps, hooks and lines, and hand harpoon respectively. A total of 47 species of fish were identified in the catches of different gears used by the fishermen in BSKB beel. Particulars, mode of operation, fishing season and catch composition of different fishing gears were determined. Seine, cast and lift net, traps (charo, arinda and ghuni), and hooks and lines (dhawn and nol broshi) were recorded as nonselective gear considering the fish species caught. However, gill nets (punti, koi and fash jal), clasp nets (bhuti jal), some traps (khadom, tubo), hooks and lines (chip borshi, chasra) and all spears were used as more or less selective gear. With respect to species and its size fash jal, bhuti jal, trap (khadom, ramani), and koach, juti and jhupi among spears were regarded to be more or less large-species-gear. But punti jal, koi jal, trap (koi dughair, charo, tubo, arinda and ghuni), nol borshi and spear (ful-kuchi) were small-species-gear. Among all gears seine net, cast net, lift net, koi dughair and ramani were recorded deleterious for carps especially for stocked fingerlings. For relatively small sized wild fishes koi jal, punti jal and ghuni traps were identified as detrimental gear.

Nutrient digestibility coefficients of diets with varying energy to protein ratio for Japanese flounder, Paralichthys olivaceus

A laboratory trial was conducted in a sea water recirculatory system to study the nutrient digestibility coefficients of diets with varying energy to protein ratios in Japanese flounder Paralicthys olivaceus. Six different experimental diets with two protein levels (45 and 55%) having six different energy to protein ratio of 87, 90, 94, 107, 110 and 114 were formulated using white fish meal and casein as protein sources. The results of the study showed that the apparent protein digestibility (APD) value ranged between 90.59 to 91.61% and there were no significant differences (P>0.05) between the APD values of diets 1, 2, 3, 4 and 6. The apparent lipid digestibility (ALD) values of diets ranged between 88.24 to 90.18%. The apparent energy digestibility (AED) values ranged between 80.55 to 87.52% with diet 3 producing significantly the highest AED value. In general, except in diet 1 the ALD and AED values increased with the increase of dietary lipid at both protein levels. The results of the present investigation indicated that Japanese flounder can efficiently digest the dietary nutrients at varying energy to protein ratios.

Effect of mixed feeding schedule on growth and nutrient utilisation in Rohu, Labeo rohita (Ham.) fingerlings

Studies on nutrient utilisation and growth of rohu (Labeo rohita) fingerlings (3.13-4.09 g) raised on seven feeding schedules under laboratory conditions (26.3-33.5°C) showed that two days regular feeding on Diet B (high protein, 38.90%) resulted in maximum growth and protein retention efficiency. While regular feeding on low protein diet (Diet A) resulted in poor nutrient utilisation and growth of fish, high protein diet (Diet B) did not show any significant difference in growth from the mixed 2A-3B schedules but exhibited comparatively low protein retention efficiency. The other feeding schedules were 1A-2B, 1A-3B, 2A-2B and 2A-4B, where the numerical value refers to the number of days for continuous feeding of a particular diet. The diets A and B served as the controls which contained 3.40 and 3.67 kcal/g gross energy respectively. The 2A-3B feeding schedule was also found to be highly economic as an expenditure of Rs.11/- only was required for raising the body weight by one kilo as against Rs.17/- with high protein diet (Diet B) as calculated

Species composition and abundance of different Macrobrachium species in a natural habitat of Kalu River near Titvala, Maharashtra State

Juveniles of freshwater prawns caught by the traditional cylindrical bamboo traps operated by the local fishermen in a natural habitat of Kalu River near Titvala were sampled every week during September to December 1991 and 1992, to study the composition and relative abundance of different species and their relationship with hydrobiological parameters. The juvenile catch comprised Macrobrachium rosenbergii, M. idella, M. scabriculum and M. bombayensis. Among these M. rosenbergii was more abundant followed by M. idella. It is also observed that high tide catches are more productive than those of low tides.

Wadge Bank trawl fishery studies. Pt. 3. Nature and composition of the resident population

The demersal stock of the Wadge Bank is known to be made up of two major groups, namely the resident and migrant stocks (Sivalingam 1966b). It is necessary to analyse the two groups separately in order to correctly interpret the changes in relative abundance of the demersal fish stocks of the Bank. The object of this paper is to present the nature of the resident stock and discuss its changes in composition from 1945 to 1960. That of the migrant population will be presented later. The significance of this analysis has been discussed earlier (Sivalingam 1966a).

Fluctuations in species composition of Penaeid prawns in estuaries

During a study of the distribution of penaeid prawns in Sri Lanka waters a difference was noticed in the species composition from one estuary to another. Moreover, a marked monthly fluctuation in the relative abundance of the different penaied species was also observed. This indicated migratory behaviour. In order to study this phenomenon in the different species of prawns, regular samples of prawns were collected from two estuaries on the south-west coast of Sri Lanka, namely at Moratuwa/Panadura and at Negombo. The investigation was spread over a period of three years from 1957-1959.

Physical variables, nutrient status and primary productivity of Kyoga Basin lakes of Lemwa, Omunuo, Gawa, Adois and Owapet

The review report on Kyoga basin lakes (NAFIRRI 2007) described Kyoga basin lakes as important natural resource for the communities within the basin and the surrounding areas. Fisheries of the basin provide a source of protein, income, and employment to generally poor communities in the area. The lakes also generate revenue to the local Governments within the catchment. This indicates that the fisheries of Kyoga basin lakes are a key instrument in poverty eradication and food security. The lakes also act as a source of water for domestic, agricultural and transport purposes. Some of the Kyoga small lakes harbour fish species, which have disappeared from the main lakes Victoria and Kyoga and are therefore important for biodiversity conservation

The freshwater zooplankton of Sri Lanka, with a discussion of tropical freshwater zooplankton composition

Based on the study of over 500 zooplankton samples collected in Sri Lanka during 1965-1974, the species composition from different habitats is analysed. The zooplankton assemblage is typically tropical with relatively few species of Cladocera and Copepoda. The Rotifera include a large number of species of the genus Brachionus. The limnetic zooplankton resembles the pond zooplankton closely in that all the eurytopic species found in the different types of habitats, including ponds; also occur in the limnetic zooplankton. The large Cladocera belonging to the genus Daphnia are very rare. In general, large zooplankters are absent. The size composition of the zooplankton has a smaller range than in temperate regions. This is due to the absence of large-sized zooplankton species. The reasons for the differences in species variety and size composition between zooplankton of temperate and tropical regions is perhaps due to a number of factors. These include the effects of high and uniform temperatures, food availability and predation by fish and invertebrates.

The morphometrics and the proximate composition of the edible fresh water mussel Lamellidens lamellatus (Lea) from Bathalagoda tank, a man made reservoir in Sri Lanka

Morphometric characteristics of Lamellidens lamellatus were studied using a random sample of 138 individuals collected from Bathalagoda reservoir in Kurunegala district. The largest number of individuals in the collection was between 40-50 mm in length. They had a body weight between 60-80 gm. The most abundant bio-chemical component in the adductor and in the foot was protein. Carbohydrate and lipid quantities were almost equal while ash showed the least value. The total protein content ranged from 48.8% to 73.4%; the carbohydrate content from 12.64% to 23.8%; and lipid content from 12.0% to 28.1% of their dry weight. It was found that there were significant relationships between the length and the body weight; and between length and volume.

A comparative study of the morphometrics and the proximate composition of two edible molluscs Crassostrea cuculata (Born) and Perna perna (L.)

The morphometrics and the bio-chemical characteristics of Crassostrea cuculata and Perna perna were studied. In the oyster, the length was found to be positively correlated with breadth and total body weight. There was also a positive relationship between the total body weight and the total muscle weight. In the mytilid the total body weight was positively correlated with length and total muscle weight. The muscle weight was also found to be highly correlated with the length. In both animals the relationship between the length and the total body weight was curvilinear. In the oyster, the protein content was found to increase with the total body weight and the total muscle weight. In the mytilid the protein content increased not only with the total body weight but also with the length. In both animals, the relative content of protein is higher than that of carbohydrates, lipids and ash.

Effect of lykamin on growth and biochemical composition of long whiskered catfish, Mystus gulio (Hamilton) fingerlings under laboratory conditions

A growth trial was conducted to test the effect of Lykamin (dietary supplement) on growth, survival and feed conversion in Mystus gulio. Four isoproteic diets were formulated by incorporating the growth promoter, Lykamin at the levels of 0, 0.25, 0.50 and 0.75% diet. The crude protein content was 30% in each diet. The diets were fed to triplicate groups of catfish fingerlings twice daily at a level of 5% of the total body weight for 120 days. Regular fortnightly sampling was done to evaluate the growth performance of the experimental animals. Water quality parameters during the experimental period were maintained within the range suitable for catfish. The results showed better specific growth rate, weight gain, protein efficiency ratio and feed conversion ratio in the group which was given 0.75% Lykamin diet.

Impact of dead and sunken water hyacinth on biotic communities, the aquatic environment and socio-economic activities

The mobile water hyacinth, which was produced in growth zones, especially Murchison Bay, was mainly exported to three sheltered storage bays (Thruston, Hannington and Waiya). Between 1996 and May 1998, the mobile form of water hyacinth occupied about 800 ha in Thruston Bay, 750 ha in Hannington Bay and 140 ha in Waiya Bay). Biological control weevils and other factors, including localised nutrient depletion, weakened the weed that was confined to the bays and it sunk around October 1998. The settling to the bottom of such huge quantities of organic matter its subsequent decomposition and the debris from this mass was likely to have environmental impacts on biotic communities (e.g. fish and invertebrate), physico-chemical conditions (water quality), and on socio-economic activities (e.g. at fish landings, water abstraction, and hydro-power generation points). Sunken water. hyacinth debris could also affect nutrient levels in the water column and lead to reduction in the content of dissolved oxygen. The changes in nutrient dynamics and oxygen levels could affect algal productivity, invertebrate composition and fish communities. Socio-economic impacts of dead sunken weed were expected from debris deposited along the shoreline especially at fish landings, water abstraction and hydropower generation points. Therefore, environmental impact assessment studies were carried out between 1998 and 2002 in selected representative zones of Lake Victoria to identify the effects of the sunken water hyacinth biomass

Effects of iso-nitrogenous fertilizers as nutrient sources on carp polyculture in Bangladesh

A 120-day long experiment was conducted to find out the effects of urea plus triple super phosphate (UT), cow manure (CM) and poultry manure (PM) having iso-nil:rogen content on pond productivity and fish yield. Three fertilizer treatments, with three replicates each, were randomly assigned into nine earthen ponds of 100 m2 each. The stocking fish were rohu (Labeo ruhita), catla ( Catla catla) and mrigal ( Cirrhinus mrigala) in each treatment pond at the rate of 10000/ha with the ratio of 1:1:1. All ponds were fertilized fortnightly at the rate of 125 kg/ha urea plus 100 kg TSP/ha, 7000 kg/ha cow manure and 3500 kg/ha poultry manure for the treatment of UT, CM and PM, respectively, having an iso-nitrogen content of 56 kg in each. Though the physicochemical water quality parameters were more or less similar in all treatment ponds, the chlorophyll-a content and abundance of total plankton were significantly higher (P < 0.05) in the ponds receiving the treatment PM. Final growth as well as per unit production of fish was significantly higher (p

Effects of iso-nitrogenous and iso-phosphorus fertilizers as nutrient sources on carp polyculture in Bangladesh

A 120 day long experiment was conducted to find out the effects of cow manure with urea and triple super phosphate (CUT), poultry manure with urea and triple super phosphate (PUT) and cow manure with poultry manure (CP) having similar quantities of nitrogen and phosphorus on pond productivity and fish yield. The stocking fish were rohu (Labeo rohita), catla ( Catla catla) and mrigal ( Cirrhinus mrigala) in each treatment pond at the rate of 10000/ha. All ponds were fertilized fortnightly at the rate of 4000 kg/ha cow manure with 62 kg/ha urea and 65 kg/ha TSP, 2700 kg/ha poultry manure with 62 kg/ha urea and 16 kg/ha TSP, and 4000kg/ha cow manure with 2700 kg/ha poultry manure for the treatment CUT, PUT and CP respectively. Each treatment contained an iso-nitrogen and iso-phosphorus of 56 kg and 46 kg respectively. Though the physico-chemical parameters were more or less similar in all ponds, the chlorophyll-a content and abundance of total plankton were significantly higher (P< 0.05) in the ponds receiving the fertilizer treatment of PUT than those of other treatments. Final growth as well as per unit production of fish of treatment PUT (1773 kg/ha) was significantly higher (P< 0.05) than that of treatment CP (1528 kg/ha) followed by that of treatment CUT (1336 kg/ha). The over all results showed that poultry manure with urea and triple super phosphate proved to be superior to cow manure with urea and triple super phosphate, and poultry manure with cow manure, even when nitrogen and phosphorus content was similar, in carp polyculture system under prevailing conditions.