Influence of formulated diets with varying protein levels on growth and ammonia excretion in the fry of Indian major carp, Cirrhina mrigala

The influence of formulated isocaloric diets of different protein levels (30, 35, 40, 45 and 50%) on the growth and ammonia excretion of the Indian major carp Cirrhina mrigala fry was studied for a rearing period of four weeks in the laboratory. Fishmeal, groundnut oilcake and silkworm pupae formed the source of protein in all the diets. As the dietary protein level increased from 30 to 40%, the growth and conversion efficiency increased significantly. Further increase in the protein level resulted in decrease in growth and conversion efficiency. Growth rate, weight gain (%), and gross and net feed conversion efficiencies were maximum at 40% dietary protein level. Ammonia excretion was directly proportional to the level of protein in the diet.

Dynamics of parasite population and its histopathological and histophysiological effects in the stomach of a freshwater fish

The caryophyllaeid cestode Lytocestoides fossilis infects the freshwater catfish Heteropneustes fossilis. The study was conducted for two consecutive years (2004-06) to record the bio-statistical data of the parasite. The incidence, intensity, density and index of infection of the parasite have been recorded. The infection was more during June to September, moderate during February to May and low during October to January. The parasite brought about severe histopathological changes in the stomach of infected fish. The changes observed in the stomach of fish included structural damage of the villi, inflammation, and fibrosis associated with hyperplasia and metaplasia. The hypertrophy of mucous layer led to vacuolation and necrosis. Histochemical changes were noticed with enhanced carbohydrate, protein and lipid contents. The enhanced substrate content in the infected organ might be due to the disfunctioning of the digestive tract, which results in the accumulation of various metabolites. Mucus secretion was triggered as a protective interaction against parasitic invasion. The parasitic infection affects the general metabolic state of the host and as the result, the fish becomes sluggish and moribund.