Development of partially hydrolysed and deodourised fish flour

A process developed for preparation of partially hydrolysed and deodorised (PHD) fish flour, without solvent extraction is described. The PHD fish flour prepared from four varieties of trash fish had creamy white colour and contained 87-92% protein and 7-8% available lysine. The products had shelf life of more than a year at room temperature, and caused no grittiness when incorporated in snacks and bakery products at 10% and 7.5% level respectively. The flour prepared from Nemipterus spp. had a PER 2.32 compared to 2.68 of raw fish meat and 2.5 of standard casein.

Haltbarkeit gefriergelagerter küchenfertiger Miesmuscheln

The aim of the project is to determine the shelf life of ready mussel products when frozen and stored at –20 ± 2 °C. Two kinds of flour were used (plain and spiced). The products were stored on styrofoam tablets and covered by stretch film at –20 ± 2 °C. During storage physical, sensorical and chemical analyses were made once a month. We found that the shelf life of the ready mussel product is 120 days dependent on sensorical, physical and chemical parameters.

Feeding and waste management strategies in the culture of Clarias gariepinus

Nutrient digestibility and amino acid availability were assessed in sharp-toothed catfish, Clarias gariepinus, fingerlings fed diets containing soyabean flour (SF) - Poultry meat meal (PMM) blends (25:75. 50:50, and 75:25) and 0.5 of 1.0%, Cr sub(2)0 sub(3). There was agreement between the pattern of overall protein digestibility and average amino acid availability despite the variability in individual amino acid availability the best dry matter, lipid and protein digestibility coefficients, and amino acid availability values were obtained with diets containing 0.5% Cr sub(2)0 sub(3). Chromic Oxide inclusion level appeared to affect nutrient availability. Increased marker level resulted into decreased nutrient digestibility coefficients. Similarly, these diets generated lower fecal crude protein than those with 1.0% Cr sub(2)0 sub(3). However, the latter group recorded higher protein retention efficiency. Dry mailer and lipid of diets containing more soyabean flour seemed to be more digestible than those of poultry meat meal. Similar trend was observed for the apparent availability of the amino acids. This investigation has indicated that low level of marker was better in digestibility study. Utilization of more SF than PMM in the diets of this catfish was more beneficial and should be encouraged in the feed industries producing catfish diets towards a better feed and waste management strategies in this aquaculture operation

Étude préliminaire sur l'incorporation de liants dans un aliment composé pour poisson d'élevage en Côte d'Ivoire

Artificial feeding constitutes a very important aspect for intensive fish culture. Then, the physical properties, particularly water stability of feed, wider influence the fish production. By trying to find some answers to these problems, a preliminary study on water stability has been conducted at the LAYO aquaculture research station. This work consisted in incorporating different types of binders available in tropical countries in fish pellets of 3.5 mm and 6.0 mm diameters in order to identify an efficient binder allowing the improvement of the water stability of feed. From this experiment, it comes out that the use of wheat flour (A1) gives excellent quality pellets followed by cassava flour residue (A3). Moreover, this study shows that it exists an influence of the diameter on the crumbling of the pellets.

Effect of dietary protein level on the growth of Deccan mahseer fry Tor khudree Sykes

Four feeds having different protein levels were separately tested on Tor khudree fry having an average length of 23.5 mm and weight 55 mg. The best average growth of 15.66 mg and 0.456 mm/day was obtained with Feed IV which comprised rice bran, ground nut oil cake, Acetes, wheat flour and mineral mix at the ratio of 1:1:1:0.7143:0.01428. These constitute 35.29% of proteins. The Feed II which comprised R.B + G.O.C. + prawn shell + wheat flour and mineral mix at a ratio of 1:1:1:0.7143:0.01428 contained 32.61% crude proteins. It provided a growth rate of 14.83 mg and 0.440 mm per day. The conversion rates were 38.258 and 37.776 for feeds IV and II respectively. Since Feed II is cheaper than Feed IV and provides almost equal growth rate, it can be used in the nurseries for intensive rearing of T. khudree fry.

Functional properties of enzymatically modified protein from fish waste

Fish flour from dried waste consisting of head, tail, fins and entrails was enzimatically hydrolysed using various proteases and the hydrolysate was spray dried. The functional properties such as water-fat absorption ratio, foaming and solubility index of the hydrolysates and fish flour revealed that some of the products might find significant uses in the food and/or cosmetics industry. Electrophoretic separation of the proteins from the fish flour and of the hydrolysates indicated that almost all the flour proteins are susceptible to proteolytic cleavage with the exception of one or two. The extent of degree of hydrolysis from 43-70.3% with a simultaneous decrease in unpleasant smell suggest an economical tool for minimizing odour pollution due to fish industry waste deterioration.

Impact of stocking density on growth and survival rate of mud crab (Scylla serrata Froskal)

A 10-weeks culture trial of mud crab, Scylla serrata in brackish water earthen pond was conducted in different stocking densities. The aim of the experiment was to identify a suitable stocking density for optimum production. There were three treatment as 5000 crab lings/ha, 10000 crab lings/ha and 15000 crab lings/ha of each with three replications. The initial mean weight of crab lings were same (5.5 ± 0.13 g). The experimental month was June '95 to August '95. The size of each pond was 500 m². To maintain good water quality water was exchanged in every spring tide. The salinity during the experiment were 2-18ppt. Prepared feed of about 32% protein consisting fish meal, MOC rice bran and wheat flour was used at 5% of their body weight. In terms of production, survival rate, growth and carapace width, the stocking density having 10000/ha showed the best (P<O.O5) performance followed by 5000/ha and 15000/ha.

Effects of cowdung application on the production of mud crab (Scylla serrata Forskal) in brackishwater pond

A culture experiment of mud crab for 84 days was conducted in earthen pond at Brackishwater Station, Bangladesh Fisheries Research Institute, Paikgacha, Khulna. The aim of the experiment was to study the effects of cowdung application on crab production and water quality. There were three treatments as without cowdung (T1), 500 kg cowdung/ha/fortnight (T2) and 750 kg cowdung/ha/fortnight (T3) with three replicates for each. The result was evaluated on the basis of growth, production, survival rate etc. The experimental months were mid-April '96 to mid-July '96. To maintain a good water quality, water was exchanged in every spring tide. The range of salinity during the experiment was 8-19 ppt. Trash fish and fresh shrimp head were used as feed on raw basis in every alternate week at a rate of 8% body weight of crab at the same time rice bran and wheat flour were used at a rate of 2% body weight as a source of carbohydrate. The production of T1, T2 and T3, were 720.35 kg, 862.16 kg and 669.19 kg/ha respectively. Though the effects of cowdung on production of crab is insignificant but in terms of production, survival rate and growth, the study suggest that the application of cowdung in addition to feed can be recommended for mud crab culture at a rate of 500 kg/ha/fortnight.

Effects of physiological doses of vitamin D sub(3) (Cholecalciferol) on induced molting and growth in giant freshwater prawn, Macrobrachium rosenbergii (de Man)

Effects of three different doses of vitamin D sub(3) on molting, growth, and calcium and phosphate composition of tissue and molt during the grow-out of the giant freshwater prawn Macrobrachium rosenbergii (average weight 10.56 ± 0.20 g), obtained from a grow-out pond, were studied. Intramuscular doses of vitamin D sub(3) (100, 500 and 2000 IU/kg body weight) were given on the 1st, 3rd, 5th, 7th, 9th, 11th, 13th, 15th, 20th, 25th and 30th days. All the experimental animals were fed with a basal diet containing fish meal, shrimp meal, wheat flour, groundnut de-oiled cake, soybean meal and wheat bran at 3% of the body weight. The numbers of molts were recorded as 20±0.50, 29±1.16, 51±1.87, and 30±1.60 in control, 100, 500 and 2000 IU/kg body weight physiological doses, respectively. Maximum growth was recorded in prawns given 500 IU/ kg dose. Survival was between 58.33 ± 9.13 and 77.77 ± 8.61%. The ash content and calcium level increased significantly (p<0.05) and recorded the highest values in 500 IU/kg physiological dose. However, the inorganic phosphate (P sub(i)) content recorded the highest values in tissue in 2000 IU/kg dose (p<0.05, F = 50.60613). There is no significant difference in calcium contents (p>0.05) in both tissue and molt at 500 and 2000 IU/kg doses. It was found that a higher physiological dose (2000 IU/kg) of vitamin D sub(3) increased the rate of mortality. Results have shown that vitamin D sub(3) has a positive impact on the growth and survival of M. rosenbergii and it interferes with the metabolism of Ca and P sub(i), in tissue, and alters molting frequency. Results on physiological dose suggest an alternative and effective dietary supplementation method of vitamin D sub(3) in the grow-out phase of M. rosenbergii.

Standardization and frozen storage of fish cutlet from bleached and unbleached mackerel mincemeat

In the present study, fish cutlets were prepared from bleached and unbleached mackerel mincemeat. Fish cutlets prepared from bleached meat had scored higher values for taste, flavour and overall acceptability as compared to those from unbleached mincemeat. Fish cutlets prepared with corn flour at the rate of 15% of fish mincemeat had scored higher values for all attributes as compared to other levels. Between the bleached and unbleached mincemeat, the scores for cutlet prepared with bleached mincemeat had higher score than that for the latter. There were no cracks in cutlets prepared with 15% and above corn flour levels as compared to those with lower levels. Fish cutlets prepared from bleached and unbleached mincemeat with spice mixture at 20 and 30% of the fish mince, respectively, had higher scores for taste, flavour, texture and overall acceptability as compared to those with other levels. Organoleptic quality of cutlet prepared from bleached and unbleached mackerel mince did not show changes in the appearance, colour and texture during storage. Changes were more prominent in flavour, taste and overall acceptability. Fish cutlets prepared from bleached mincemeat were acceptable for two months and those from unbleached mincemeat were acceptable up to one month from the point of view of organoleptic and biochemical qualities.

Preparation of an extruded fish snack using twin screw extruder and the storage characteristics of the product

A value-added extruded fish product was prepared with corn flour (80%) and fish (sciaenid) powder (20%), using a twin-screw extruder. The effect of different parameters like moisture, temperature, fish powder concentration, speed of the extruder and die-diameter on expansion ratio and crisp texture were studied. The storage characteristics of the final product were studied using three different types of packaging under nitrogen flushing. The study revealed that aluminum foil is the best packaging material to keep the product acceptable for more than three months.

Input-output relationship and economics of pangas monoculture and carp-pangas polyculture in two districts of Bangladesh

An attempt was made to study the input-output relationships and economics of pangas monoculture and carp-pangas polyculture in Bangladesh. By analyzing the data collected from 50 pangas farms and 55 carp-pangas farms, the study has investigated the production systems of two technologies and the effects of fingerling stocking and applications of feed and fertilizer on fisheries income. The data were collected from the fishermen of Trishal and Bhaluka of Mymensingh district, and Kahaloo and Adamdighee of Bogra district during 2001-02. For pangas monoculture, the stocking density was 31,561 per ha while it was 55,017 per ha in carp-pangas polyculture. Most of the farmers used urea, TSP and lime before stocking. Rice and wheat bran happened to be the most common feed ingredients for both types of culture in general. Other important ingredients used were mustard oil-cakes, rice polish, wheat flour, fish meal, bone meal, soybean meal and poultry litter. In terms of quantities, rice bran and wheat bran dominated the farmers list. Rice and wheat bran together constituted about 60% of all studied feeds. Feed cost constituted 59.13% of total costs for pangas monoculture and 67.44% for carp-pangas polyculture. Per ha productions of pangas and carp-pangas in a single culture cycle were 15,508 kg and 19,745 kg, respectively. Per ha gross profits were estimated to be Tk 310,311 and Tk 464,418 for pangas monoculture and carp-pangas polyculture, respectively. Net profit appeared to be Tk 264,216 per ha for pangas monoculture and Tk 416,509 per ha for carp-pangas polyculture. The BCRs calculated were 1.46 and 1.68 for monoculture and polyculture, respectively. The break-even costs per kg of fish were estimated at Tk 36.93 for pangas and Tk 30.93 for mixed species which was much lower than the prices the producers received. Break-even productions were estimated at 10,702 kg per ha for pangas monoculture and 11,784 kg per ha for carp-pangas polyculture. Fingerling and feed cost, and pond size significantly explained the variation of income from pangas monoculture. These factors have significantly influenced the income from the crop. Functional analysis shows that 1% increase in the feed cost might increase 0.51% of pangas income and 0.41% in carp-pangas income. No other inputs had shown this much of responses to increasing income from a fish.

Preliminary chemical and physical evaluation of some formulated feeds for P. monodon

The culture of Penaeus monodon has explicitly defined the need for diet formulations or supplementary feeds that would promote optimum growth and survival of the animal. A total of 28 feed combinations were developed for P. monodon. Fish meal, shrimp head meal, squid head meal, Ascetes spp. rice bran, and soybean cake were used as primary ingredients in these feeds. The commercial vitamin mix No. 22 was added to the dry ingredients. Gelatinized corn starch and wheat flour were used as binders. The pellets were extruded using a portable kitchen grinder with a diameter of 4 mm. The products were either sun-dried for 8 hours or oven-dried overnight at 50 degree C to stabilize moisture at 8-10%. The pellets were then kept in covered glass bottles and stored in the laboratory at room temperature. The cost of the feeds excluding labour were also computed. The pellets were analyzed for protein, fat, carbohydrate, crude fiber, ash, and moisture contents using standard procedures. They were also analyzed for water stability. To test the stability of pellets in water, 2-g samples were placed in plankton nets (mesh #40) and suspended in water for two, and six hours. The undissolved samples were then vacuum-dried and the moisture determined. Cost of the feeds ranged from P1.10 to P2.60 per kg depending on the feed ingredient. Squid and Ascetes spp. were rather expensive for use as basic ingredients. Proximate analysis of dry weight showed percentage protein content ranged from 20-63 g; fat, 8-20 g; carbohydrate (by difference), 11-36 g; ash, 8-28 g; moisture, 6-11 g; and crude fiber, 5 . 13 g. Stability tests showed that after two hours, 35-88% of solids remained intact and after 6 hours, 20-55% of the pellets remained undissolved. When a pellet disintegrates easily, pollution of the water occurs. Chances for the shrimp to feed on the pellet is minimized when the pellet is unstable. Thus, the search for a more compact feed pellet has to be continued.

The effect of different binders on the water stability of feeds for prawn

Corn starch, gelatin, sago palm starch, agar, and bread flour were tested for their binding capacity in pelleted diets for Penaeus monodon . Agar was found to be good binder, but it costs too much, while bread flour was also good but as it's commonly used for human comsumption its use for animal feed should be minimized. The use of 20% bread flour, or a combination of 5% sago palm starch or corn starch with 15% bread flour is recommended, depending on the cost and availability. Basic composition of the formulated diet is tabulated, as is water stability of 2 and 4 mm diameter steamed pellets after 2, 6 and 18 h.

The water stability of shrimp diets with various polysaccharides as a binding agent

The water stability of shrimp (Penaeus monodon) diets with various polysaccharides as binding agents were tested without steaming. The diet with alginate showed the best water stability and did not completely disintegrate in 24 hours. However, the use of alginate is dependent on cost and availability, so alternate choices may be a combination of sago palm starch and wheat flour or glutinous rice flour.