1 resultado para real power loss
em National Center for Biotechnology Information - NCBI
Filtro por publicador
- Academic Archive On-line (Stockholm University; Sweden) (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (9)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
- Archive of European Integration (6)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (7)
- Aston University Research Archive (36)
- Biblioteca Digital | Sistema Integrado de Documentación | UNCuyo - UNCUYO. UNIVERSIDAD NACIONAL DE CUYO. (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (12)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (4)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (17)
- Boston University Digital Common (1)
- Brock University, Canada (3)
- Bucknell University Digital Commons - Pensilvania - USA (3)
- Bulgarian Digital Mathematics Library at IMI-BAS (5)
- CaltechTHESIS (7)
- Cambridge University Engineering Department Publications Database (33)
- CentAUR: Central Archive University of Reading - UK (29)
- Central European University - Research Support Scheme (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (24)
- Cochin University of Science & Technology (CUSAT), India (4)
- Coffee Science - Universidade Federal de Lavras (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (2)
- CORA - Cork Open Research Archive - University College Cork - Ireland (5)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (2)
- CUNY Academic Works (2)
- Deakin Research Online - Australia (57)
- Digital Commons - Michigan Tech (6)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (19)
- Digital Peer Publishing (1)
- DigitalCommons@University of Nebraska - Lincoln (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (4)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (6)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (2)
- Greenwich Academic Literature Archive - UK (2)
- Harvard University (3)
- Helda - Digital Repository of University of Helsinki (4)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (76)
- INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES (IPEN) - Repositório Digital da Produção Técnico Científica - BibliotecaTerezine Arantes Ferra (1)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (41)
- Iowa Publications Online (IPO) - State Library, State of Iowa (Iowa), United States (1)
- Memoria Académica - FaHCE, UNLP - Argentina (6)
- National Center for Biotechnology Information - NCBI (1)
- Nottingham eTheses (1)
- Publishing Network for Geoscientific & Environmental Data (1)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (89)
- Queensland University of Technology - ePrints Archive (120)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (2)
- Repositório Científico da Universidade de Évora - Portugal (4)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (4)
- Repositorio de la Universidad de Cuenca (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (8)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositorio Institucional de la Universidad Pública de Navarra - Espanha (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (106)
- Research Open Access Repository of the University of East London. (2)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- The Scholarly Commons | School of Hotel Administration; Cornell University Research (1)
- Universidad de Alicante (4)
- Universidad del Rosario, Colombia (9)
- Universidad Politécnica de Madrid (58)
- Universidade Complutense de Madrid (1)
- Universidade de Lisboa - Repositório Aberto (2)
- Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) (1)
- Universidade Federal do Pará (2)
- Universidade Federal do Rio Grande do Norte (UFRN) (3)
- Universidade Metodista de São Paulo (4)
- Universitat de Girona, Spain (3)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (1)
- Université de Montréal, Canada (2)
- University of Canberra Research Repository - Australia (1)
- University of Connecticut - USA (2)
- University of Michigan (2)
- University of Queensland eSpace - Australia (14)
- University of Southampton, United Kingdom (3)
- University of Washington (2)
- WestminsterResearch - UK (1)
Resumo:
A hybrid protein, tPA/GFP, consisting of rat tissue plasminogen activator (tPA) and green fluorescent protein (GFP) was expressed in PC12 cells and used to study the distribution, secretory behavior, and dynamics of secretory granules containing tPA in living cells with a neuronal phenotype. High-resolution images demonstrate that tPA/GFP has a growth cone-biased distribution in differentiated cells and that tPA/GFP is transported in granules of the regulated secretory pathway that colocalize with granules containing secretogranin II. Time-lapse images of secretion reveal that secretagogues induce substantial loss of cellular tPA/GFP fluorescence, most importantly from growth cones. Time-lapse images of the axonal transport of granules containing tPA/GFP reveal a surprising complexity to granule dynamics. Some granules undergo canonical fast axonal transport; others move somewhat more slowly, especially in highly fluorescent neurites. Most strikingly, granules traffic bidirectionally along neurites to an extent that depends on granule accumulation, and individual granules can reverse their direction of motion. The retrograde component of this bidirectional transport may help to maintain cellular homeostasis by transporting excess tPA/GFP back toward the cell body. The results presented here provide a novel view of the axonal transport of secretory granules. In addition, the results suggest that tPA is targeted for regulated secretion from growth cones of differentiated cells, strategically positioning tPA to degrade extracellular barriers or to activate other barrier-degrading proteases during axonal elongation.