1 resultado para Non-competitive labor markets
em National Center for Biotechnology Information - NCBI
Filtro por publicador
- KUPS-Datenbank - Universität zu Köln - Kölner UniversitätsPublikationsServer (2)
- Aberdeen University (1)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- Academic Research Repository at Institute of Developing Economies (28)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (12)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Andina Digital - Repositorio UASB-Digital - Universidade Andina Simón Bolívar (1)
- Aquatic Commons (2)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (5)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (51)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (6)
- Aston University Research Archive (25)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (5)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (1)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (2)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (17)
- Boston University Digital Common (2)
- Brock University, Canada (8)
- Cambridge University Engineering Department Publications Database (1)
- CentAUR: Central Archive University of Reading - UK (36)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (5)
- Cochin University of Science & Technology (CUSAT), India (1)
- Coffee Science - Universidade Federal de Lavras (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (22)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (7)
- Dalarna University College Electronic Archive (5)
- Deakin Research Online - Australia (47)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (3)
- Digital Archives@Colby (1)
- Digital Commons - Michigan Tech (2)
- Digital Commons - Montana Tech (1)
- Digital Commons at Florida International University (15)
- Digital Peer Publishing (1)
- DigitalCommons@The Texas Medical Center (7)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- DRUM (Digital Repository at the University of Maryland) (4)
- Duke University (3)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (2)
- Fachlicher Dokumentenserver Paedagogik/Erziehungswissenschaften (1)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Greenwich Academic Literature Archive - UK (6)
- Helda - Digital Repository of University of Helsinki (8)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (10)
- Instituto Politécnico do Porto, Portugal (43)
- Memoria Académica - FaHCE, UNLP - Argentina (15)
- Memorial University Research Repository (1)
- National Center for Biotechnology Information - NCBI (1)
- Portal de Revistas Científicas Complutenses - Espanha (4)
- Publishing Network for Geoscientific & Environmental Data (7)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (8)
- Queensland University of Technology - ePrints Archive (44)
- Repositorio Academico Digital UANL (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório Científico da Universidade de Évora - Portugal (4)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (4)
- Repositorio de la Universidad de Cuenca (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (34)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositório do Centro Hospitalar de Lisboa Central, EPE - Centro Hospitalar de Lisboa Central, EPE, Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (2)
- Repositório Institucional da Universidade de Brasília (1)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (1)
- Repositório Institucional da Universidade Federal do Rio Grande do Norte (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (26)
- Repositorio Institucional Universidad EAFIT - Medelin - Colombia (2)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (5)
- SerWisS - Server für Wissenschaftliche Schriften der Fachhochschule Hannover (2)
- Universidad de Alicante (3)
- Universidad del Rosario, Colombia (32)
- Universidad Politécnica de Madrid (8)
- Universidade Complutense de Madrid (3)
- Universidade Federal do Pará (2)
- Universidade Federal do Rio Grande do Norte (UFRN) (6)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (3)
- Université de Lausanne, Switzerland (2)
- Université de Montréal (2)
- Université de Montréal, Canada (31)
- Université Laval Mémoires et thèses électroniques (2)
- University of Connecticut - USA (7)
- University of Michigan (22)
- University of Queensland eSpace - Australia (10)
- University of Washington (5)
- WestminsterResearch - UK (1)
- Worcester Research and Publications - Worcester Research and Publications - UK (2)
Resumo:
The recent demonstration of the occurrence in rat brain and other nonpancreatic tissues of carboxypeptidase A (CPA) gene transcripts without associated catalytic activity could be ascribed to the presence of a soluble endogenous protein inhibitor. This tissue carboxypeptidase inhibitor (TCI), detected by the inhibition of added bovine pancreatic CPA, was purified from rat brain. Peptides were obtained by partial proteolysis of purified TCI, a protein of approximately 30 kDa, and starting from their sequences, a full-length cDNA encoding a 223-amino acid protein containing three potential phosphorylation sites was cloned from a cDNA library. Its identity with TCI was shown by expression in Escherichia coli of a recombinant protein recognized by antibodies raised against native TCI and display characteristic CPA-inhibiting activity. TCI appears as a hardly reversible, non-competitive, and potent inhibitor of CPA1 and CPA2 (Ki approximately 3 nM) and mast-cell CPA (Ki = 16 nM) and inactive on various other proteases. This pattern of selectivity might be attributable to a limited homology of a 11-amino acid sequence with sequences within the activation segments of CPA and CPB known to interact with residues within their active sites. The widespread expression of TCI in a number of tissues (e.g., brain, lung, or digestive tract) and its apparently cytosolic localization point to a rather general functional role, e.g., in the control of cytosolic protein degradation.