1 resultado para Media and minorities
em National Center for Biotechnology Information - NCBI
Filtro por publicador
- JISC Information Environment Repository (1)
- Repository Napier (2)
- Aberdeen University (2)
- Aberystwyth University Repository - Reino Unido (1)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- Adam Mickiewicz University Repository (2)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (8)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Aquatic Commons (1)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (2)
- Archive of European Integration (16)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (1)
- Aston University Research Archive (20)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (5)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (3)
- Biblioteca Digital de la Universidad Católica Argentina (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (33)
- Brock University, Canada (7)
- Cambridge University Engineering Department Publications Database (7)
- CentAUR: Central Archive University of Reading - UK (33)
- Center for Jewish History Digital Collections (1)
- Central European University - Research Support Scheme (2)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (8)
- Cochin University of Science & Technology (CUSAT), India (4)
- Coffee Science - Universidade Federal de Lavras (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (2)
- CORA - Cork Open Research Archive - University College Cork - Ireland (1)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (1)
- CUNY Academic Works (1)
- Dalarna University College Electronic Archive (4)
- Deakin Research Online - Australia (162)
- Digital Commons - Michigan Tech (4)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (1)
- Digital Peer Publishing (2)
- DigitalCommons@The Texas Medical Center (6)
- DigitalCommons@University of Nebraska - Lincoln (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- Duke University (3)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (3)
- Glasgow Theses Service (1)
- Greenwich Academic Literature Archive - UK (2)
- Helda - Digital Repository of University of Helsinki (20)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (2)
- Indian Institute of Science - Bangalore - Índia (6)
- Instituto Politécnico do Porto, Portugal (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- National Center for Biotechnology Information - NCBI (1)
- Nottingham eTheses (1)
- Open Access Repository of Association for Learning Technology (ALT) (1)
- Open University Netherlands (2)
- Portal de Revistas Científicas Complutenses - Espanha (3)
- Publishing Network for Geoscientific & Environmental Data (1)
- QSpace: Queen's University - Canada (2)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (39)
- Queensland University of Technology - ePrints Archive (354)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (6)
- Repositório Institucional da Universidade de Aveiro - Portugal (3)
- Repositorio Institucional de la Universidad de Málaga (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (33)
- Repositorio Institucional Universidad de Medellín (1)
- Research Open Access Repository of the University of East London. (2)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Universidad de Alicante (5)
- Universidad del Rosario, Colombia (1)
- Universidad Politécnica de Madrid (10)
- Universidade de Lisboa - Repositório Aberto (3)
- Universidade Federal do Pará (1)
- Universidade Metodista de São Paulo (2)
- Universitat de Girona, Spain (1)
- Université de Lausanne, Switzerland (1)
- Université de Montréal, Canada (1)
- University of Michigan (18)
- University of Queensland eSpace - Australia (41)
- University of Southampton, United Kingdom (2)
- University of Washington (2)
- WestminsterResearch - UK (16)
- Worcester Research and Publications - Worcester Research and Publications - UK (3)
Resumo:
Bacterial shape usually is dictated by the peptidoglycan layer of the cell wall. In this paper, we show that the morphology of the Lyme disease spirochete Borrelia burgdorferi is the result of a complex interaction between the cell cylinder and the internal periplasmic flagella. B. burgdorferi has a bundle of 7–11 helically shaped periplasmic flagella attached at each end of the cell cylinder and has a flat-wave cell morphology. Backward moving, propagating waves enable these bacteria to swim in both low viscosity media and highly viscous gel-like media. Using targeted mutagenesis, we inactivated the gene encoding the major periplasmic flagellar filament protein FlaB. The resulting flaB mutants not only were nonmotile, but were rod-shaped. Western blot analysis indicated that FlaB was no longer synthesized, and electron microscopy revealed that the mutants were completely deficient in periplasmic flagella. Wild-type cells poisoned with the protonophore carbonyl cyanide-m-chlorophenylhydrazone retained their flat-wave morphology, indicating that the periplasmic flagella do not need to be energized for the cell to maintain this shape. Our results indicate that the periplasmic flagella of B. burgdorferi have a skeletal function. These organelles dynamically interact with the rod-shaped cell cylinder to enable the cell to swim, and to confer in part its flat-wave morphology.