1 resultado para Knowledge construction
em National Center for Biotechnology Information - NCBI
Filtro por publicador
- Abertay Research Collections - Abertay University’s repository (1)
- Aberystwyth University Repository - Reino Unido (2)
- Academic Archive On-line (Mid Sweden University; Sweden) (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (2)
- Archive of European Integration (1)
- Aston University Research Archive (13)
- B-Digital - Universidade Fernando Pessoa - Portugal (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (1)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (10)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (7)
- Brock University, Canada (2)
- Bulgarian Digital Mathematics Library at IMI-BAS (5)
- Cambridge University Engineering Department Publications Database (3)
- CentAUR: Central Archive University of Reading - UK (69)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (3)
- CORA - Cork Open Research Archive - University College Cork - Ireland (2)
- Dalarna University College Electronic Archive (3)
- Deakin Research Online - Australia (81)
- Department of Computer Science E-Repository - King's College London, Strand, London (1)
- Digital Archives@Colby (1)
- Digital Commons - Michigan Tech (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (4)
- Digital Peer Publishing (2)
- DigitalCommons@The Texas Medical Center (2)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (1)
- Escola Superior de Educação de Paula Frassinetti (3)
- Greenwich Academic Literature Archive - UK (1)
- Helda - Digital Repository of University of Helsinki (2)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (2)
- Instituto de Engenharia Nuclear, Brazil - Carpe dIEN (1)
- Instituto Politécnico de Santarém (2)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (4)
- Massachusetts Institute of Technology (1)
- Memoria Académica - FaHCE, UNLP - Argentina (10)
- Ministerio de Cultura, Spain (3)
- National Center for Biotechnology Information - NCBI (1)
- Open University Netherlands (2)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (22)
- Queensland University of Technology - ePrints Archive (528)
- RCAAP - Repositório Científico de Acesso Aberto de Portugal (1)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositório Aberto da Universidade Aberta de Portugal (1)
- Repositorio Académico de la Universidad Nacional de Costa Rica (2)
- Repositório Científico da Universidade de Évora - Portugal (2)
- Repositório Científico do Instituto Politécnico de Santarém - Portugal (2)
- Repositório digital da Fundação Getúlio Vargas - FGV (2)
- Repositório Institucional da Universidade de Aveiro - Portugal (4)
- Repositório Institucional da Universidade de Brasília (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (43)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Savoirs UdeS : plateforme de diffusion de la production intellectuelle de l’Université de Sherbrooke - Canada (1)
- SerWisS - Server für Wissenschaftliche Schriften der Fachhochschule Hannover (1)
- The Scholarly Commons | School of Hotel Administration; Cornell University Research (1)
- Universidad de Alicante (4)
- Universidad del Rosario, Colombia (1)
- Universidad Politécnica de Madrid (8)
- Universidade de Lisboa - Repositório Aberto (4)
- Universidade Federal do Pará (6)
- Universidade Federal do Rio Grande do Norte (UFRN) (10)
- Universidade Metodista de São Paulo (5)
- Universitat de Girona, Spain (3)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (1)
- Université de Lausanne, Switzerland (1)
- Université de Montréal (1)
- Université de Montréal, Canada (9)
- University of Michigan (4)
- University of Queensland eSpace - Australia (5)
- University of Washington (5)
- WestminsterResearch - UK (2)
Resumo:
We have genetically replaced the native receptor binding domain of diphtheria toxin with an extended form of substance P (SP): SP-glycine (SP-Gly). The resulting fusion protein, DAB389SP-Gly, is composed of the catalytic and transmembrane domains of diphtheria toxin genetically coupled to SP-Gly. Because native SP requires a C-terminal amide moiety to bind with high affinity to the SP receptor, the precursor form of the fusion toxin, DAB389SP-Gly, was converted to DAB389SP by treatment with peptidylglycine-alpha-amidating monooxygenase. We demonstrate that following conversion, DAB389SP is selectively cytotoxic for cell lines that express either the rat or the human SP receptor. We also demonstrate that the cytotoxic action of DAB389SP is mediated via the SP receptor and dependent upon passage through an acidic compartment. To our knowledge, this is the first reported use of a neuropeptide as the targeting ligand for a fusion toxin; and the first instance in which an inactive precursor form of a fusion toxin is converted to the active form by a posttranslational modification.