1 resultado para Building blocks in elastomer composite fabrication
em National Center for Biotechnology Information - NCBI
Filtro por publicador
- Aberystwyth University Repository - Reino Unido (1)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- Adam Mickiewicz University Repository (2)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (9)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (7)
- Aquatic Commons (2)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (13)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (2)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (5)
- Aston University Research Archive (10)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (9)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (36)
- Boston University Digital Common (2)
- Brock University, Canada (13)
- Bucknell University Digital Commons - Pensilvania - USA (2)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- CaltechTHESIS (13)
- Cambridge University Engineering Department Publications Database (47)
- CentAUR: Central Archive University of Reading - UK (38)
- Center for Jewish History Digital Collections (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (79)
- Cochin University of Science & Technology (CUSAT), India (22)
- Coffee Science - Universidade Federal de Lavras (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (4)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (1)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (57)
- Department of Computer Science E-Repository - King's College London, Strand, London (1)
- Digital Commons - Michigan Tech (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (9)
- DigitalCommons@The Texas Medical Center (1)
- Diposit Digital de la UB - Universidade de Barcelona (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (2)
- DRUM (Digital Repository at the University of Maryland) (3)
- Duke University (3)
- Düsseldorfer Dokumenten- und Publikationsservice (1)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (5)
- Glasgow Theses Service (2)
- Helda - Digital Repository of University of Helsinki (16)
- Indian Institute of Science - Bangalore - Índia (112)
- INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES (IPEN) - Repositório Digital da Produção Técnico Científica - BibliotecaTerezine Arantes Ferra (1)
- Instituto Politécnico de Bragança (1)
- Instituto Politécnico do Porto, Portugal (1)
- Iowa Publications Online (IPO) - State Library, State of Iowa (Iowa), United States (1)
- Massachusetts Institute of Technology (3)
- Ministerio de Cultura, Spain (7)
- National Aerospace Laboratory (NLR) Reports Repository (1)
- National Center for Biotechnology Information - NCBI (1)
- Nottingham eTheses (2)
- Portal de Revistas Científicas Complutenses - Espanha (2)
- Publishing Network for Geoscientific & Environmental Data (7)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (109)
- Queensland University of Technology - ePrints Archive (180)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (2)
- Repositório digital da Fundação Getúlio Vargas - FGV (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (3)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (17)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (6)
- Scientific Open-access Literature Archive and Repository (1)
- Universidad de Alicante (3)
- Universidad del Rosario, Colombia (1)
- Universidad Politécnica de Madrid (13)
- Universidade de Lisboa - Repositório Aberto (2)
- Universidade Federal do Pará (1)
- Universidade Federal do Rio Grande do Norte (UFRN) (1)
- Universita di Parma (2)
- Universitat de Girona, Spain (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (5)
- Université de Montréal, Canada (11)
- Université Laval Mémoires et thèses électroniques (1)
- University of Canberra Research Repository - Australia (1)
- University of Michigan (17)
- University of Queensland eSpace - Australia (8)
- University of Washington (5)
- WestminsterResearch - UK (4)
Resumo:
To create a universal system for the control of gene expression, we have studied methods for the construction of novel polydactyl zinc finger proteins that recognize extended DNA sequences. Elsewhere we have described the generation of zinc finger domains recognizing sequences of the 5′-GNN-3′ subset of a 64-member zinc finger alphabet. Here we report on the use of these domains as modular building blocks for the construction of polydactyl proteins specifically recognizing 9- or 18-bp sequences. A rapid PCR assembly method was developed that, together with this predefined set of zinc finger domains, provides ready access to 17 million novel proteins that bind the 5′-(GNN)6-3′ family of 18-bp DNA sites. To examine the efficacy of this strategy in gene control, the human erbB-2 gene was chosen as a model. A polydactyl protein specifically recognizing an 18-bp sequence in the 5′-untranslated region of this gene was converted into a transcriptional repressor by fusion with Krüppel-associated box (KRAB), ERD, or SID repressor domains. Transcriptional activators were generated by fusion with the herpes simplex VP16 activation domain or with a tetrameric repeat of VP16’s minimal activation domain, termed VP64. We demonstrate that both gene repression and activation can be achieved by targeting designed proteins to a single site within the transcribed region of a gene. We anticipate that gene-specific transcriptional regulators of the type described here will find diverse applications in gene therapy, functional genomics, and the generation of transgenic organisms.