16 resultados para preference ordering
Filtro por publicador
- Aberdeen University (1)
- Aberystwyth University Repository - Reino Unido (2)
- Academic Research Repository at Institute of Developing Economies (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (2)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (4)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Aquatic Commons (11)
- Archive of European Integration (2)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (3)
- Aston University Research Archive (15)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (4)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (5)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (45)
- Brock University, Canada (9)
- Bucknell University Digital Commons - Pensilvania - USA (4)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- CaltechTHESIS (1)
- Cambridge University Engineering Department Publications Database (16)
- CentAUR: Central Archive University of Reading - UK (42)
- Central European University - Research Support Scheme (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (37)
- Cochin University of Science & Technology (CUSAT), India (2)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (6)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (6)
- Deakin Research Online - Australia (70)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons @ Winthrop University (1)
- Digital Commons at Florida International University (4)
- DigitalCommons@The Texas Medical Center (1)
- DigitalCommons@University of Nebraska - Lincoln (1)
- Digitale Sammlungen - Goethe-Universität Frankfurt am Main (1)
- Diposit Digital de la UB - Universidade de Barcelona (4)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (1)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (14)
- Greenwich Academic Literature Archive - UK (2)
- Helda - Digital Repository of University of Helsinki (8)
- Indian Institute of Science - Bangalore - Índia (86)
- Instituto Gulbenkian de Ciência (1)
- Instituto Politécnico do Porto, Portugal (3)
- Massachusetts Institute of Technology (3)
- Memorial University Research Repository (2)
- National Center for Biotechnology Information - NCBI (16)
- Nottingham eTheses (1)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (1)
- Publishing Network for Geoscientific & Environmental Data (17)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (53)
- Queensland University of Technology - ePrints Archive (317)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (4)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (54)
- Research Open Access Repository of the University of East London. (1)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- School of Medicine, Washington University, United States (6)
- Universidad de Alicante (2)
- Universidad Politécnica de Madrid (4)
- Universidade Complutense de Madrid (1)
- Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) (1)
- Universidade Federal do Pará (1)
- Universidade Federal do Rio Grande do Norte (UFRN) (2)
- Universidade Técnica de Lisboa (1)
- Universitat de Girona, Spain (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (2)
- Université de Montréal, Canada (7)
- University of Connecticut - USA (2)
- University of Michigan (16)
- University of Queensland eSpace - Australia (19)
- University of Southampton, United Kingdom (1)
- University of Washington (1)
- WestminsterResearch - UK (1)
- Worcester Research and Publications - Worcester Research and Publications - UK (1)
Resumo:
Temperature-sensitive alleles in four genes (slu7-1, prp16-2, prp17-1, and prp18-1) are known to confer a specific block to the second chemical step of pre-mRNA splicing in vivo in the yeast Saccharomyces cerevisiae. Previous studies showed that Prp16p and Prp18p are required solely for the second step in vitro. The RNA-dependent ATPase, Prp16p, functions at a stage in splicing when ATP is required, whereas Prp18p functions at an ATP-independent stage. Here we use immunodepletion to show that the roles of Slu7p and Prp17p are also confined to the second step of splicing. We find that extracts depleted of Prp17p require both Prp17p and ATP for slicing complementation, whereas extracts depleted of Slu7p require only the addition of Slu7p. These different ATP requirements suggest that Prp16p and Prp17p function before Prp18p and Slu7p. Although SLU7 encodes an essential gene product, we find that a null allele of prp17 is temperature-sensitive for growth and has a partial splicing defect in vitro. Finally, high-copy suppression experiments indicate functional interactions between PRP16 and PRP17, PRP16 and SLU7, and SLU7 and PRP18. Taken together, the results suggest that these four factors may function within a multi-component complex that has both an ATP-dependent and an ATP-independent role in the second step of pre-mRNA splicing.